HepyC.18123.a

Biotin carboxylase

CENTER ID: HepyC.18123.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.18123.a.B1.GE41202 full length 1 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1118
UniProt: B5Z881

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKRNKKVEK KELSRILIAN RGEIALRAIQ TIQEMGKESI AIYSIADKDA HYLNTASAKV CIGGAKSSES YLNIPAIISA AELFEADAIF PGYGFLSENQ NFVEICSHHS LEFIGPSAKV MALMSDKSKA KSVMKEAGMP VIEGSEGLLK SYQEAEEIAD KIGYPVIIKA AAGGGGRGMR VVEDKSKLKN LYLAAETEAL SAFGDGSVYL EKFINKPKHI EVQILADKHG NVIHVGERDC SVQRRQQKLI EETPAVVLEE GVRERLLETA IKAAKYIGYV GAGTFEFLLD SNMKDFYFME MNTRLQVEHT ISEMVSGLNL IEWMIKIAQG EELPKQESFS LKGHAIECRI TAEDPKKFYP SPGKITEWIA PGGVNVRLDS HAHANYVVPT HYDSMIGKLI VWGENRERAI AKMKRALKEF KVEGIKTTIP FHLEMLENAD FRQAKIHTKY LEENF
NT Sequence
atgaataaaa gaaataaaaa ggtagaaaaa aaagagcttt cacgcatttt gatcgctaat agaggcgaga tcgctttaag agcgatccaa accattcaag aaatgggtaa agaatccata gccatttatt ctatcgctga caaagacgcc cactacctca atacggctag tgcaaaagtg tgtatagggg gggccaaatc cagcgagagt tacttgaata tccctgcgat cattagcgcg gcggaattgt ttgaagcgga tgcgattttc cccgggtatg ggtttttgag tgaaaaccag aattttgtag agatttgctc gcaccattct ttggaattta ttggtccgag cgcaaaagtc atggctttaa tgagcgataa atccaaagcc aaaagcgtga tgaaagaagc cggcatgcct gtgattgagg gcagtgaagg gttgcttaaa agctatcaag aagctgaaga aatcgctgat aaaatcggct accctgttat cattaaagcg gccgctggtg ggggcggaag ggggatgcgt gtcgtagaag ataaatccaa gcttaaaaac ctttatttag ccgcagaaac ggaagctttg agcgcgtttg gcgatgggag cgtgtattta gaaaaattca tcaacaagcc taagcacatt gaagtccaaa ttctagccga taagcatggc aatgtcattc atgtgggcga aagggattgc tccgtgcaaa gacgccagca aaagctcatt gaagaaaccc cggcggtggt tttagaagag ggcgtgcgtg agcgtttgct agaaacagcg attaaggccg ctaaatatat cggctatgtg ggggctggga cttttgaatt tttgcttgat tctaacatga aagattttta tttcatggag atgaacactc gtttgcaagt ggaacacacc attagcgaaa tggtgagcgg gttaaacctc attgaatgga tgattaaaat cgctcaaggc gaagaactgc ccaagcaaga aagcttttcc ctcaaagggc atgcgataga atgccgaatc actgcagaag atcctaaaaa attctaccca agcccgggca aaatcaccga atggatcgcc cctggtgggg tgaatgtgcg ccttgattcg cacgcgcatg ccaattatgt cgtgcctacg cactatgatt ctatgattgg caagctcatt gtgtggggtg aaaacagaga aagagcgatc gctaaaatga aaagggcttt aaaggaattt aaagtagaag gtattaaaac gaccattcct ttccaccttg aaatgcttga aaatgcggat ttcaggcaag caaaaatcca cacgaagtat ttagaagaaa atttttaa
Details for HepyC.18123.a.B1.GE41202
HARVESTED ON: data unavailable
SEQUENCED ON: 3/2/2017
EXPECTED MW: 52kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN KRNKKVEKKE LSRILIANRG EIALRAIQTI QEMGKESIAI YSIADKDAHY LNTASAKVCI GGAKSSESYL NIPAIISAAE LFEADAIFPG YGFLSENQNF VEICSHHSLE FIGPSAKVMA LMSDKSKAKS VMKEAGMPVI EGSEGLLKSY QEAEEIADKI GYPVIIKAAA GGGGRGMRVV EDKSKLKNLY LAAETEALSA FGDGSVYLEK FINKPKHIEV QILADKHGNV IHVGERDCSV QRRQQKLIEE TPAVVLEEGV RERLLETAIK AAKYIGYVGA GTFEFLLDSN MKDFYFMEMN TRLQVEHTIS EMVSGLNLIE WMIKIAQGEE LPKQESFSLK GHAIECRITA EDPKKFYPSP GKITEWIAPG GVNVRLDSHA HANYVVPTHY DSMIGKLIVW GENRERAIAK MKRALKEFKV EGIKTTIPFH LEMLENADFR QAKIHTKYLE ENF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata aaagaaataa aaaggtagaa aaaaaagagc tttcacgcat tttgatcgct aatagaggcg agatcgcttt aagagcgatc caaaccattc aagaaatggg taaagaatcc atagccattt attctatcgc tgacaaagac gcccactacc tcaatacggc tagtgcaaaa gtgtgtatag ggggggccaa atccagcgag agttacttga atatccctgc gatcattagc gcggcggaat tgtttgaagc ggatgcgatt ttccccgggt atgggttttt gagtgaaaac cagaattttg tagagatttg ctcgcaccat tctttggaat ttattggtcc gagcgcaaaa gtcatggctt taatgagcga taaatccaaa gccaaaagcg tgatgaaaga agccggcatg cctgtgattg agggcagtga agggttgctt aaaagctatc aagaagctga agaaatcgct gataaaatcg gctaccctgt tatcattaaa gcggccgctg gtgggggcgg aagggggatg cgtgtcgtag aagataaatc caagcttaaa aacctttatt tagccgcaga aacggaagct ttgagcgcgt ttggcgatgg gagcgtgtat ttagaaaaat tcatcaacaa gcctaagcac attgaagtcc aaattctagc cgataagcat ggcaatgtca ttcatgtggg cgaaagggat tgctccgtgc aaagacgcca gcaaaagctc attgaagaaa ccccggcggt ggttttagaa gagggcgtgc gtgagcgttt gctagaaaca gcgattaagg ccgctaaata tatcggctat gtgggggctg ggacttttga atttttgctt gattctaaca tgaaagattt ttatttcatg gagatgaaca ctcgtttgca agtggaacac accattagcg aaatggtgag cgggttaaac ctcattgaat ggatgattaa aatcgctcaa ggcgaagaac tgcccaagca agaaagcttt tccctcaaag ggcatgcgat agaatgccga atcactgcag aagatcctaa aaaattctac ccaagcccgg gcaaaatcac cgaatggatc gcccctggtg gggtgaatgt gcgccttgat tcgcacgcgc atgccaatta tgtcgtgcct acgcactatg attctatgat tggcaagctc attgtgtggg gtgaaaacag agaaagagcg atcgctaaaa tgaaaagggc tttaaaggaa tttaaagtag aaggtattaa aacgaccatt cctttccacc ttgaaatgct tgaaaatgcg gatttcaggc aagcaaaaat ccacacgaag tatttagaag aaaatttttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat