SSGCID: Target Page

HepyC.18062.a

DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I)

CENTER ID:	HepyC.18062.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.18062.a.B1.GE41201	full length		1	736

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.18062.a.B1.PS38285	full length		1	736

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.122
UniProt:	B5Z9P7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKHLIIVESP AKAKTIKNFL DKNYEVIASK GHVRDLSKFA LGIKIDETGF TPNYVVDKDH KELVKQIIEL SKKASITYIA TDEDREGEAI GYHVACLIGG KLESYPRIVF HEITQNAILN ALKTPRKIDM FKVNAQQARR FLDRIVGFKL SSLISSKITK GLSAGRVQSA ALKLVIDRER EIKAFKPLTY FTLDAYFESH LEAQLISYKG NKLKAQELID EKKAQEIKNE LEKESYTISS IVKKSKKSPT PPPFMTSTLQ QSASSLLGFS PTKTMSIAQK LYEGVATPQG VMGVITYMRT DSLNIAKEAL EEARAKILKD YGKDYLPPKA KVYSSKNKNA QEAHEAIRPT SIILEPNALK DYLKPEELRL YTLIYKRFLA SQMQDALFES QSVVVACEKG EFKASGRKLL FDGYYKILGN DDKDKLLPNL KENDPIKLEK LESNAHVTEP PARYSEASLI KVLESLGIGR PSTYAPTISL LQNRDYIKVE KKQISALESA FKVIEILEKH FEEIVDSKFS ASLEEELDNI AQNKADYQQV LKDFYYPFMD KIEAGKKNII SQKVHEKTGQ SCPKCGGELV KKNSRYGEFI ACNNYPKCKY VKQTENTNDG ANQELCEKCG GEMVQKFSRN GAFLACNNYP ECKNTKSLKN TPNAKEIIEG VKCPECGGDI ALKRSKKGSF YGCNNYPKCN FLSNHKPINK RCEKCHYLMS ERIYRKKKAH ECIKCKECMF LEEDNG

NT Sequence

atgaagcacc ttattattgt agaatcccca gcaaaagcca aaaccattaa aaattttttg gataaaaatt acgaagtcat cgcctctaaa gggcatgtta gggatttatc caaattcgct ttaggcatta agattgatga aacaggcttt acccctaatt atgtcgtgga taaagaccat aaagagcttg tcaaacagat catagagctt tctaaaaaag catctattac ttatatcgct accgatgaag acagagaggg ggaagcgata ggttatcatg tggcatgttt gattgggggg aaattggaga gctatcctag gattgttttt catgagatca cgcaaaatgc gattttaaac gctctaaaaa ccccacgaaa aattgacatg tttaaggtca atgcccaaca agccaggcgt tttttagatc gaatcgtggg ttttaagctc agttcgttga tttcatcaaa gatcactaaa ggcttgagcg ctgggcgggt gcaaagcgcg gctttaaagc ttgtgattga tagagaaagg gaaatcaaag cctttaagcc tttaacctac ttcacgctag acgcttattt tgagtcgcat ttagaagcgc aactcattag ctataagggc aacaaactca aagcccaaga gcttattgat gaaaaaaaag cccaagagat taaaaatgaa ctggaaaaag aaagctacac tatctctagt atcgttaaaa agtctaaaaa atcccccaca ccgccccctt tcatgacttc tactttacag caaagcgctt ccagtctttt aggcttttcg cccacaaaaa ccatgagtat cgctcaaaaa ttatatgaag gcgtagccac cccacaaggg gttatggggg tgatcactta catgagaacc gatagcttga atatcgctaa agaggcttta gaagaagcga gagctaagat tttaaaagac tatggcaaag actacctacc ccctaaagcc aaagtctatt ccagcaagaa taaaaacgcc caagaagccc atgaagcgat caggcccact tctattattt tagagccaaa cgctttaaaa gactacctta agcctgaaga attaaggctc tatactttaa tttacaaacg ctttttagct tctcaaatgc aagacgctct ttttgaaagc caaagcgtgg ttgtggcttg cgaaaaaggc gagtttaagg ctagtgggag aaagctcctt tttgatggct attataaaat tttaggcaat gacgataagg acaaattgct ccccaatttg aaagaaaatg accccattaa attagaaaaa ctagagagca acgcccatgt tacagaacct ccagcacgct attcagaagc gagcttgatt aaagttttag aaagtttagg cataggcagg cccagcacct acgccccaac gatttccctc ttgcaaaaca gagactatat taaggtagaa aaaaagcaaa tcagcgcttt agagagcgcg tttaaggtga tagaaatttt agaaaagcat tttgaagaaa tcgtggattc caaattcagc gcttctttag aagaggaatt ggataatatc gctcaaaata aagccgacta ccagcaagtc ttaaaggact tttactaccc ttttatggat aaaattgaag ccgggaaaaa gaatatcatc tctcaaaaag tgcatgaaaa aaccggccaa tcatgcccta aatgcggtgg ggaattagtc aaaaaaaata gccgttatgg ggagtttatc gcttgcaaca attaccctaa atgcaaatac gtcaaacaga ctgaaaatac caatgatgga gccaatcaag aattgtgcga aaaatgcgga ggggaaatgg tgcaaaaatt cagcagaaac ggggcgtttt tagcttgcaa caattaccct gaatgcaaaa acaccaaatc gctaaagaac acccctaacg caaaagaaat aatagaaggc gtgaaatgcc cagaatgcgg gggggatatt gccttaaaaa ggagtaagaa aggctcgttt tatggctgca acaattaccc taaatgcaat tttttatcca accataagcc catcaacaag cgttgcgaga aatgccatta tttgatgagt gaaagaatct atcgcaaaaa aaaggcgcat gaatgcatta aatgcaaaga gtgcatgttt ttagaggaag ataatggcta a

Details for HepyC.18062.a.B1.GE41201

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	84kDa
OBSERVED MW:	84kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK HLIIVESPAK AKTIKNFLDK NYEVIASKGH VRDLSKFALG IKIDETGFTP NYVVDKDHKE LVKQIIELSK KASITYIATD EDREGEAIGY HVACLIGGKL ESYPRIVFHE ITQNAILNAL KTPRKIDMFK VNAQQARRFL DRIVGFKLSS LISSKITKGL SAGRVQSAAL KLVIDREREI KAFKPLTYFT LDAYFESHLE AQLISYKGNK LKAQELIDEK KAQEIKNELE KESYTISSIV KKSKKSPTPP PFMTSTLQQS ASSLLGFSPT KTMSIAQKLY EGVATPQGVM GVITYMRTDS LNIAKEALEE ARAKILKDYG KDYLPPKAKV YSSKNKNAQE AHEAIRPTSI ILEPNALKDY LKPEELRLYT LIYKRFLASQ MQDALFESQS VVVACEKGEF KASGRKLLFD GYYKILGNDD KDKLLPNLKE NDPIKLEKLE SNAHVTEPPA RYSEASLIKV LESLGIGRPS TYAPTISLLQ NRDYIKVEKK QISALESAFK VIEILEKHFE EIVDSKFSAS LEEELDNIAQ NKADYQQVLK DFYYPFMDKI EAGKKNIISQ KVHEKTGQSC PKCGGELVKK NSRYGEFIAC NNYPKCKYVK QTENTNDGAN QELCEKCGGE MVQKFSRNGA FLACNNYPEC KNTKSLKNTP NAKEIIEGVK CPECGGDIAL KRSKKGSFYG CNNYPKCNFL SNHKPINKRC EKCHYLMSER IYRKKKAHEC IKCKECMFLE EDNG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc accttattat tgtagaatcc ccagcaaaag ccaaaaccat taaaaatttt ttggataaaa attacgaagt catcgcctct aaagggcatg ttagggattt atccaaattc gctttaggca ttaagattga tgaaacaggc tttaccccta attatgtcgt ggataaagac cataaagagc ttgtcaaaca gatcatagag ctttctaaaa aagcatctat tacttatatc gctaccgatg aagacagaga gggggaagcg ataggttatc atgtggcatg tttgattggg gggaaattgg agagctatcc taggattgtt tttcatgaga tcacgcaaaa tgcgatttta aacgctctaa aaaccccacg aaaaattgac atgtttaagg tcaatgccca acaagccagg cgttttttag atcgaatcgt gggttttaag ctcagttcgt tgatttcatc aaagatcact aaaggcttga gcgctgggcg ggtgcaaagc gcggctttaa agcttgtgat tgatagagaa agggaaatca aagcctttaa gcctttaacc tacttcacgc tagacgctta ttttgagtcg catttagaag cgcaactcat tagctataag ggcaacaaac tcaaagccca agagcttatt gatgaaaaaa aagcccaaga gattaaaaat gaactggaaa aagaaagcta cactatctct agtatcgtta aaaagtctaa aaaatccccc acaccgcccc ctttcatgac ttctacttta cagcaaagcg cttccagtct tttaggcttt tcgcccacaa aaaccatgag tatcgctcaa aaattatatg aaggcgtagc caccccacaa ggggttatgg gggtgatcac ttacatgaga accgatagct tgaatatcgc taaagaggct ttagaagaag cgagagctaa gattttaaaa gactatggca aagactacct accccctaaa gccaaagtct attccagcaa gaataaaaac gcccaagaag cccatgaagc gatcaggccc acttctatta ttttagagcc aaacgcttta aaagactacc ttaagcctga agaattaagg ctctatactt taatttacaa acgcttttta gcttctcaaa tgcaagacgc tctttttgaa agccaaagcg tggttgtggc ttgcgaaaaa ggcgagttta aggctagtgg gagaaagctc ctttttgatg gctattataa aattttaggc aatgacgata aggacaaatt gctccccaat ttgaaagaaa atgaccccat taaattagaa aaactagaga gcaacgccca tgttacagaa cctccagcac gctattcaga agcgagcttg attaaagttt tagaaagttt aggcataggc aggcccagca cctacgcccc aacgatttcc ctcttgcaaa acagagacta tattaaggta gaaaaaaagc aaatcagcgc tttagagagc gcgtttaagg tgatagaaat tttagaaaag cattttgaag aaatcgtgga ttccaaattc agcgcttctt tagaagagga attggataat atcgctcaaa ataaagccga ctaccagcaa gtcttaaagg acttttacta cccttttatg gataaaattg aagccgggaa aaagaatatc atctctcaaa aagtgcatga aaaaaccggc caatcatgcc ctaaatgcgg tggggaatta gtcaaaaaaa atagccgtta tggggagttt atcgcttgca acaattaccc taaatgcaaa tacgtcaaac agactgaaaa taccaatgat ggagccaatc aagaattgtg cgaaaaatgc ggaggggaaa tggtgcaaaa attcagcaga aacggggcgt ttttagcttg caacaattac cctgaatgca aaaacaccaa atcgctaaag aacaccccta acgcaaaaga aataatagaa ggcgtgaaat gcccagaatg cgggggggat attgccttaa aaaggagtaa gaaaggctcg ttttatggct gcaacaatta ccctaaatgc aattttttat ccaaccataa gcccatcaac aagcgttgcg agaaatgcca ttatttgatg agtgaaagaa tctatcgcaa aaaaaaggcg catgaatgca ttaaatgcaa agagtgcatg tttttagagg aagataatgg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for HepyC.18062.a.B1.PS38285

PURIFICATION DATe:	5/23/2017
CONCENTRATION:	27.1mg/ml
OBSERVED MW:	98kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC ACCTTATTAT TGTAGAATCC CCAGCAAAAG CCAAAACCAT TAAAAATTTT TTGGATAAAA ATTACGAAGT CATCGCCTCT AAAGGGCATG TTAGGGATTT ATCCAAATTC GCTTTAGGCA TTAAGATTGA TGAAACAGGC TTTACCCCTA ATTATGTCGT GGATAAAGAC CATAAAGAGC TTGTCAAACA GATCATAGAG CTTTCTAAAA AAGCATCTAT TACTTATATC GCTACCGATG AAGACAGAGA GGGGGAAGCG ATAGGTTATC ATGTGGCATG TTTGATTGGG GGGAAATTGG AGAGCTATCC TAGGATTGTT TTTCATGAGA TCACGCAAAA TGCGATTTTA AACGCTCTAA AAACCCCACG AAAAATTGAC ATGTTTAAGG TCAATGCCCA ACAAGCCAGG CGTTTTTTAG ATCGAATCGT GGGTTTTAAG CTCAGTTCGT TGATTTCATC AAAGATCACT AAAGGCTTGA GCGCTGGGCG GGTGCAAAGC GCGGCTTTAA AGCTTGTGAT TGATAGAGAA AGGGAAATCA AAGCCTTTAA GCCTTTAACC TACTTCACGC TAGACGCTTA TTTTGAGTCG CATTTAGAAG CGCAACTCAT TAGCTATAAG GGCAACAAAC TCAAAGCCCA AGAGCTTATT GATGAAAAAA AAGCCCAAGA GATTAAAAAT GAACTGGAAA AAGAAAGCTA CACTATCTCT AGTATCGTTA AAAAGTCTAA AAAATCCCCC ACACCGCCCC CTTTCATGAC TTCTACTTTA CAGCAAAGCG CTTCCAGTCT TTTAGGCTTT TCGCCCACAA AAACCATGAG TATCGCTCAA AAATTATATG AAGGCGTAGC CACCCCACAA GGGGTTATGG GGGTGATCAC TTACATGAGA ACCGATAGCT TGAATATCGC TAAAGAGGCT TTAGAAGAAG CGAGAGCTAA GATTTTAAAA GACTATGGCA AAGACTACCT ACCCCCTAAA GCCAAAGTCT ATTCCAGCAA GAATAAAAAC GCCCAAGAAG CCCATGAAGC GATCAGGCCC ACTTCTATTA TTTTAGAGCC AAACGCTTTA AAAGACTACC TTAAGCCTGA AGAATTAAGG CTCTATACTT TAATTTACAA ACGCTTTTTA GCTTCTCAAA TGCAAGACGC TCTTTTTGAA AGCCAAAGCG TGGTTGTGGC TTGCGAAAAA GGCGAGTTTA AGGCTAGTGG GAGAAAGCTC CTTTTTGATG GCTATTATAA AATTTTAGGC AATGACGATA AGGACAAATT GCTCCCCAAT TTGAAAGAAA ATGACCCCAT TAAATTAGAA AAACTAGAGA GCAACGCCCA TGTTACAGAA CCTCCAGCAC GCTATTCAGA AGCGAGCTTG ATTAAAGTTT TAGAAAGTTT AGGCATAGGC AGGCCCAGCA CCTACGCCCC AACGATTTCC CTCTTGCAAA ACAGAGACTA TATTAAGGTA GAAAAAAAGC AAATCAGCGC TTTAGAGAGC GCGTTTAAGG TGATAGAAAT TTTAGAAAAG CATTTTGAAG AAATCGTGGA TTCCAAATTC AGCGCTTCTT TAGAAGAGGA ATTGGATAAT ATCGCTCAAA ATAAAGCCGA CTACCAGCAA GTCTTAAAGG ACTTTTACTA CCCTTTTATG GATAAAATTG AAGCCGGGAA AAAGAATATC ATCTCTCAAA AAGTGCATGA AAAAACCGGC CAATCATGCC CTAAATGCGG TGGGGAATTA GTCAAAAAAA ATAGCCGTTA TGGGGAGTTT ATCGCTTGCA ACAATTACCC TAAATGCAAA TACGTCAAAC AGACTGAAAA TACCAATGAT GGAGCCAATC AAGAATTGTG CGAAAAATGC GGAGGGGAAA TGGTGCAAAA ATTCAGCAGA AACGGGGCGT TTTTAGCTTG CAACAATTAC CCTGAATGCA AAAACACCAA ATCGCTAAAG AACACCCCTA ACGCAAAAGA AATAATAGAA GGCGTGAAAT GCCCAGAATG CGGGGGGGAT ATTGCCTTAA AAAGGAGTAA GAAAGGCTCG TTTTATGGCT GCAACAATTA CCCTAAATGC AATTTTTTAT CCAACCATAA GCCCATCAAC AAGCGTTGCG AGAAATGCCA TTATTTGATG AGTGAAAGAA TCTATCGCAA AAAAAAGGCG CATGAATGCA TTAAATGCAA AGAGTGCATG TTTTTAGAGG AAGATAATGG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT