HepyC.18004.a
Fumarate reductase. flavo protein subunit
| CENTER ID: | HepyC.18004.a |
| ORGANISM: | Helicobacter pylori G27 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.18004.a.B1.GE41200 | full length | 1 | 714 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.18004.a.B1.PS38425 | full length | 1 | 714 |
Structures
6N56
DEPOSITED: 11/21/2018
DETERMINATION: XRay
CLONE: HepyC.18004.a.B1.GE41200
PROTEIN: HepyC.18004.a.B1.PS38425
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|563041.6.peg.199 |
| UniProt: | B5Z9W3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKITYCDALI IGGGLAGLRA SIACKQKGLN TIVLSLVPVR RSHSAAAQGG MQASLANAKK SEGDNEDLHF LDTVKGSDWG CDQQVARMFV TTAPKAIREL ASWGVPWTRI KKGDRPAVVN GEHVIITERD DRHGYILSRD FGGTKKWRTC FTADATGHTM LYAVANEALH HKVDIQDRKD MLAFIHHDNK CYGAVVRDLI TGEISAYVSK GTLLATGGYG RVYKHTTNAV ICDGAGAASA LETGVAKLGN MEAVQFHPTA LVPSGILMTE GCRGDGGVLR DKFGRRFMPA YEPEKKELAS RDVVSRRILE HIQKGYGAKS PYGDHVWLDI AILGRNHVEK NLRDVRDIAM TFAGIDPADS EEQTKDNMQG MPANEPEYGQ AMAKQKGWIP IKPMQHYSMG GVRTNPKGET HLKGLFCAGE AACWDLHGFN RLGGNSVSEA VVAGMIIGDY FASHCLEAQI EINTQKVEAF IKESQDYMHF LLHNEGKEDV YEIRERMKEV MDEKVGVFRE GKKLEEALKE LQELYARSKN ICVKNKVLHN NPELEDAYRT KKMLKLALCI TQGALLRTES RGAHTRIDYP KRDDEKWLNR TLASWPSTEQ DMPTIEYEEL DVMKMEISPD FRGYGKKGNF IPHPKKEERD AEILKTILEL EKLGKDRIEV QHALMPFELQ EKYKARNMRL EDEEVRARGE HLYSFNVHDL LDQHNANLKG EHHE
NT Sequence
atgaaaataa catattgtga tgcgttgatt attggaggcg gattggccgg gttaagagcc agtatcgcat gcaaacaaaa gggtttaaac accatcgttt taagcctagt gcctgtcagg cgttcgcatt ctgcggccgc tcaagggggc atgcaagcga gccttgcgaa cgctaaaaaa agcgagggcg ataatgaaga tttgcacttt ttagacacgg ttaaggggag cgattggggg tgtgatcagc aagtggctag gatgtttgta accactgccc ctaaagccat tagggaattg gccagttggg gggtgccttg gactaggatt aaaaagggcg ataggcctgc ggtcgtcaat ggtgagcatg ttatcattac cgaaagagac gataggcatg gttatatctt aagccgtgat tttggcggca ctaaaaaatg gcgcacatgt tttacggctg atgctacagg gcataccatg ctttatgcgg tcgctaatga agccttacac cacaaagtgg atattcaaga cagaaaggac atgctcgctt tcattcatca tgataataaa tgctatgggg cggtggtaag ggatttgatc acaggcgaaa tttcagcgta tgtttctaaa ggcacgcttt tagctactgg aggttatgga cgcgtgtata aacacaccac taacgccgtg atttgcgatg gagccggggc ggcgagcgct ttagaaaccg gcgtggctaa attaggcaac atggaagcgg tgcaattcca ccctaccgct ttagtgccaa gcgggatttt aatgaccgaa ggttgcaggg gcgatggcgg ggttttaaga gacaagtttg gcagacgctt catgcccgct tatgagccgg agaaaaaaga gcttgcgagt agggatgtgg tctcaaggcg gattttagag catatccaaa aaggctatgg agccaaatcg ccctatgggg atcatgtgtg gctggatatt gctattttag ggcgtaacca tgtggaaaaa aatttaaggg atgtgcgcga tattgccatg acttttgcgg gcattgatcc ggctgatagc gaggaacaaa ccaaagacaa catgcaagga atgcccgcaa atgagcctga atacgggcaa gcgatggcta agcaaaaagg ttggatcccc ataaaaccca tgcaacacta ttctatgggt ggggttagga caaaccctaa aggcgaaacc catttaaaag gcttgttttg cgcgggtgaa gcagcatgct gggatttgca tgggtttaac cgcttggggg gaaattctgt gagtgaagcg gtggtcgctg gcatgattat aggggattat tttgcgtcgc attgtttgga agcgcaaatt gaaatcaaca cgcaaaaggt tgaagccttc attaaagaaa gccaggatta tatgcatttt ttattgcaca atgaaggcaa ggaagacgtg tatgaaatca gagaacgcat gaaagaagtc atggatgaaa aagtgggcgt ttttagagaa ggcaaaaaat tagaagaagc ccttaaagaa ttgcaagagc tttatgcacg ctccaaaaac atttgcgtga aaaacaaggt tttgcacaat aaccctgaat tagaagacgc ttaccgcacc aaaaaaatgc tcaaactcgc gctttgcatc actcaaggag cgttactgcg cactgaaagc agaggggctc acacaaggat tgattaccct aaaagagacg atgaaaaatg gcttaatcgg actttagcga gctggcctag cactgagcaa gacatgccca cgattgaata cgaagaatta gatgtgatga aaatggaaat cagccctgat tttaggggct atggcaaaaa gggcaatttc attcctcacc ccaaaaaaga agagcgcgac gctgagattt tgaaaacgat tttagaatta gaaaagcttg gaaaagacag gatagaagtc caacatgcgc tcatgccttt tgaattgcaa gaaaaataca aagccaggaa catgcgttta gaagatgagg aagtcagggc taggggggaa catttgtatt ctttcaacgt ccatgattta ttagaccaac acaacgctaa cctaaaagga gaacaccatg agtga
Details for HepyC.18004.a.B1.GE41200
| HARVESTED ON: | 3/1/2017 |
| SEQUENCED ON: | 3/2/2017 |
| EXPECTED MW: | 81kDa |
| OBSERVED MW: | 81kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK ITYCDALIIG GGLAGLRASI ACKQKGLNTI VLSLVPVRRS HSAAAQGGMQ ASLANAKKSE GDNEDLHFLD TVKGSDWGCD QQVARMFVTT APKAIRELAS WGVPWTRIKK GDRPAVVNGE HVIITERDDR HGYILSRDFG GTKKWRTCFT ADATGHTMLY AVANEALHHK VDIQDRKDML AFIHHDNKCY GAVVRDLITG EISAYVSKGT LLATGGYGRV YKHTTNAVIC DGAGAASALE TGVAKLGNME AVQFHPTALV PSGILMTEGC RGDGGVLRDK FGRRFMPAYE PEKKELASRD VVSRRILEHI QKGYGAKSPY GDHVWLDIAI LGRNHVEKNL RDVRDIAMTF AGIDPADSEE QTKDNMQGMP ANEPEYGQAM AKQKGWIPIK PMQHYSMGGV RTNPKGETHL KGLFCAGEAA CWDLHGFNRL GGNSVSEAVV AGMIIGDYFA SHCLEAQIEI NTQKVEAFIK ESQDYMHFLL HNEGKEDVYE IRERMKEVMD EKVGVFREGK KLEEALKELQ ELYARSKNIC VKNKVLHNNP ELEDAYRTKK MLKLALCITQ GALLRTESRG AHTRIDYPKR DDEKWLNRTL ASWPSTEQDM PTIEYEELDV MKMEISPDFR GYGKKGNFIP HPKKEERDAE ILKTILELEK LGKDRIEVQH ALMPFELQEK YKARNMRLED EEVRARGEHL YSFNVHDLLD QHNANLKGEH HE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa taacatattg tgatgcgttg attattggag gcggattggc cgggttaaga gccagtatcg catgcaaaca aaagggttta aacaccatcg ttttaagcct agtgcctgtc aggcgttcgc attctgcggc cgctcaaggg ggcatgcaag cgagccttgc gaacgctaaa aaaagcgagg gcgataatga agatttgcac tttttagaca cggttaaggg gagcgattgg gggtgtgatc agcaagtggc taggatgttt gtaaccactg cccctaaagc cattagggaa ttggccagtt ggggggtgcc ttggactagg attaaaaagg gcgataggcc tgcggtcgtc aatggtgagc atgttatcat taccgaaaga gacgataggc atggttatat cttaagccgt gattttggcg gcactaaaaa atggcgcaca tgttttacgg ctgatgctac agggcatacc atgctttatg cggtcgctaa tgaagcctta caccacaaag tggatattca agacagaaag gacatgctcg ctttcattca tcatgataat aaatgctatg gggcggtggt aagggatttg atcacaggcg aaatttcagc gtatgtttct aaaggcacgc ttttagctac tggaggttat ggacgcgtgt ataaacacac cactaacgcc gtgatttgcg atggagccgg ggcggcgagc gctttagaaa ccggcgtggc taaattaggc aacatggaag cggtgcaatt ccaccctacc gctttagtgc caagcgggat tttaatgacc gaaggttgca ggggcgatgg cggggtttta agagacaagt ttggcagacg cttcatgccc gcttatgagc cggagaaaaa agagcttgcg agtagggatg tggtctcaag gcggatttta gagcatatcc aaaaaggcta tggagccaaa tcgccctatg gggatcatgt gtggctggat attgctattt tagggcgtaa ccatgtggaa aaaaatttaa gggatgtgcg cgatattgcc atgacttttg cgggcattga tccggctgat agcgaggaac aaaccaaaga caacatgcaa ggaatgcccg caaatgagcc tgaatacggg caagcgatgg ctaagcaaaa aggttggatc cccataaaac ccatgcaaca ctattctatg ggtggggtta ggacaaaccc taaaggcgaa acccatttaa aaggcttgtt ttgcgcgggt gaagcagcat gctgggattt gcatgggttt aaccgcttgg ggggaaattc tgtgagtgaa gcggtggtcg ctggcatgat tataggggat tattttgcgt cgcattgttt ggaagcgcaa attgaaatca acacgcaaaa ggttgaagcc ttcattaaag aaagccagga ttatatgcat tttttattgc acaatgaagg caaggaagac gtgtatgaaa tcagagaacg catgaaagaa gtcatggatg aaaaagtggg cgtttttaga gaaggcaaaa aattagaaga agcccttaaa gaattgcaag agctttatgc acgctccaaa aacatttgcg tgaaaaacaa ggttttgcac aataaccctg aattagaaga cgcttaccgc accaaaaaaa tgctcaaact cgcgctttgc atcactcaag gagcgttact gcgcactgaa agcagagggg ctcacacaag gattgattac cctaaaagag acgatgaaaa atggcttaat cggactttag cgagctggcc tagcactgag caagacatgc ccacgattga atacgaagaa ttagatgtga tgaaaatgga aatcagccct gattttaggg gctatggcaa aaagggcaat ttcattcctc accccaaaaa agaagagcgc gacgctgaga ttttgaaaac gattttagaa ttagaaaagc ttggaaaaga caggatagaa gtccaacatg cgctcatgcc ttttgaattg caagaaaaat acaaagccag gaacatgcgt ttagaagatg aggaagtcag ggctaggggg gaacatttgt attctttcaa cgtccatgat ttattagacc aacacaacgc taacctaaaa ggagaacacc atgagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for HepyC.18004.a.B1.PS38425
| PURIFICATION DATe: | 3/13/2018 |
| CONCENTRATION: | 31.4mg/ml |
| OBSERVED MW: | 90kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK ITYCDALIIG GGLAGLRASI ACKQKGLNTI VLSLVPVRRS HSAAAQGGMQ ASLANAKKSE GDNEDLHFLD TVKGSDWGCD QQVARMFVTT APKAIRELAS WGVPWTRIKK GDRPAVVNGE HVIITERDDR HGYILSRDFG GTKKWRTCFT ADATGHTMLY AVANEALHHK VDIQDRKDML AFIHHDNKCY GAVVRDLITG EISAYVSKGT LLATGGYGRV YKHTTNAVIC DGAGAASALE TGVAKLGNME AVQFHPTALV PSGILMTEGC RGDGGVLRDK FGRRFMPAYE PEKKELASRD VVSRRILEHI QKGYGAKSPY GDHVWLDIAI LGRNHVEKNL RDVRDIAMTF AGIDPADSEE QTKDNMQGMP ANEPEYGQAM AKQKGWIPIK PMQHYSMGGV RTNPKGETHL KGLFCAGEAA CWDLHGFNRL GGNSVSEAVV AGMIIGDYFA SHCLEAQIEI NTQKVEAFIK ESQDYMHFLL HNEGKEDVYE IRERMKEVMD EKVGVFREGK KLEEALKELQ ELYARSKNIC VKNKVLHNNP ELEDAYRTKK MLKLALCITQ GALLRTESRG AHTRIDYPKR DDEKWLNRTL ASWPSTEQDM PTIEYEELDV MKMEISPDFR GYGKKGNFIP HPKKEERDAE ILKTILELEK LGKDRIEVQH ALMPFELQEK YKARNMRLED EEVRARGEHL YSFNVHDLLD QHNANLKGEH HE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA TAACATATTG TGATGCGTTG ATTATTGGAG GCGGATTGGC CGGGTTAAGA GCCAGTATCG CATGCAAACA AAAGGGTTTA AACACCATCG TTTTAAGCCT AGTGCCTGTC AGGCGTTCGC ATTCTGCGGC CGCTCAAGGG GGCATGCAAG CGAGCCTTGC GAACGCTAAA AAAAGCGAGG GCGATAATGA AGATTTGCAC TTTTTAGACA CGGTTAAGGG GAGCGATTGG GGGTGTGATC AGCAAGTGGC TAGGATGTTT GTAACCACTG CCCCTAAAGC CATTAGGGAA TTGGCCAGTT GGGGGGTGCC TTGGACTAGG ATTAAAAAGG GCGATAGGCC TGCGGTCGTC AATGGTGAGC ATGTTATCAT TACCGAAAGA GACGATAGGC ATGGTTATAT CTTAAGCCGT GATTTTGGCG GCACTAAAAA ATGGCGCACA TGTTTTACGG CTGATGCTAC AGGGCATACC ATGCTTTATG CGGTCGCTAA TGAAGCCTTA CACCACAAAG TGGATATTCA AGACAGAAAG GACATGCTCG CTTTCATTCA TCATGATAAT AAATGCTATG GGGCGGTGGT AAGGGATTTG ATCACAGGCG AAATTTCAGC GTATGTTTCT AAAGGCACGC TTTTAGCTAC TGGAGGTTAT GGACGCGTGT ATAAACACAC CACTAACGCC GTGATTTGCG ATGGAGCCGG GGCGGCGAGC GCTTTAGAAA CCGGCGTGGC TAAATTAGGC AACATGGAAG CGGTGCAATT CCACCCTACC GCTTTAGTGC CAAGCGGGAT TTTAATGACC GAAGGTTGCA GGGGCGATGG CGGGGTTTTA AGAGACAAGT TTGGCAGACG CTTCATGCCC GCTTATGAGC CGGAGAAAAA AGAGCTTGCG AGTAGGGATG TGGTCTCAAG GCGGATTTTA GAGCATATCC AAAAAGGCTA TGGAGCCAAA TCGCCCTATG GGGATCATGT GTGGCTGGAT ATTGCTATTT TAGGGCGTAA CCATGTGGAA AAAAATTTAA GGGATGTGCG CGATATTGCC ATGACTTTTG CGGGCATTGA TCCGGCTGAT AGCGAGGAAC AAACCAAAGA CAACATGCAA GGAATGCCCG CAAATGAGCC TGAATACGGG CAAGCGATGG CTAAGCAAAA AGGTTGGATC CCCATAAAAC CCATGCAACA CTATTCTATG GGTGGGGTTA GGACAAACCC TAAAGGCGAA ACCCATTTAA AAGGCTTGTT TTGCGCGGGT GAAGCAGCAT GCTGGGATTT GCATGGGTTT AACCGCTTGG GGGGAAATTC TGTGAGTGAA GCGGTGGTCG CTGGCATGAT TATAGGGGAT TATTTTGCGT CGCATTGTTT GGAAGCGCAA ATTGAAATCA ACACGCAAAA GGTTGAAGCC TTCATTAAAG AAAGCCAGGA TTATATGCAT TTTTTATTGC ACAATGAAGG CAAGGAAGAC GTGTATGAAA TCAGAGAACG CATGAAAGAA GTCATGGATG AAAAAGTGGG CGTTTTTAGA GAAGGCAAAA AATTAGAAGA AGCCCTTAAA GAATTGCAAG AGCTTTATGC ACGCTCCAAA AACATTTGCG TGAAAAACAA GGTTTTGCAC AATAACCCTG AATTAGAAGA CGCTTACCGC ACCAAAAAAA TGCTCAAACT CGCGCTTTGC ATCACTCAAG GAGCGTTACT GCGCACTGAA AGCAGAGGGG CTCACACAAG GATTGATTAC CCTAAAAGAG ACGATGAAAA ATGGCTTAAT CGGACTTTAG CGAGCTGGCC TAGCACTGAG CAAGACATGC CCACGATTGA ATACGAAGAA TTAGATGTGA TGAAAATGGA AATCAGCCCT GATTTTAGGG GCTATGGCAA AAAGGGCAAT TTCATTCCTC ACCCCAAAAA AGAAGAGCGC GACGCTGAGA TTTTGAAAAC GATTTTAGAA TTAGAAAAGC TTGGAAAAGA CAGGATAGAA GTCCAACATG CGCTCATGCC TTTTGAATTG CAAGAAAAAT ACAAAGCCAG GAACATGCGT TTAGAAGATG AGGAAGTCAG GGCTAGGGGG GAACATTTGT ATTCTTTCAA CGTCCATGAT TTATTAGACC AACACAACGC TAACCTAAAA GGAGAACACC ATGAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT