HepyC.17943.a

Leucine--tRNA ligase (EC 6.1.1.4) (Leucyl-tRNA synthetase)

CENTER ID: HepyC.17943.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17943.a.B1.GE41185 full length 1 806
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17943.a.B1.PW38402 full length 1 806

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1611
UniProt: B5Z9H8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFINIEKKW QEFWWKNKSF EPKDDFNLPK KYILSMLPYP SGEIHMGHVR NYTIGDALAR YYRLHHYNVL HPMGFDSFGM PAENAAIKHG IHPKTWTYEN IEAMQKEFEA LGFSFSKNRE FATSDPDYTK FEQQFFIDLW EKGLIYRKKA MLNWCPNDKT VLANEQVIDG RCWRCDTEVI QKELYQYYLK ITNYAEELLK DLETLENHWP SQVLIMQKNW IGKSSGLQFG FKIADECLKA CNGIQEIEVF TTRADTIYGV TYIAIAPEHP LVEHAIKRVS QEDSKIIKAI LNTTQRERAL EKKGAFLGVY AIHPLTKQKI PVWVANFALA NYGSGALMGV PACDERDFEF ANLYHIPIKV ITQSPQNLPH TKEEVLKNSG EWSDLSSSVA REKIIAYFEK ENLGKRVINY RLQDWGVSRQ RYWGAPIPMI HCKHCGIVPE TQLPVTLPED IVIDGEGNPL EKHASWKFAQ CPKCHKDALR ETDTMDTFIQ SSWYFLRYTT PKNQRENQAF DQNYLKYFMP VDTYIGGIEH AILHLLYARF FTKALRDLGY IHLDEPFKQL ITQGMVLKNG TKMSKSKGNV VSPKEILKKY GADAARLFIL FAAPPAKELE WNDSALEGAH RFIKRLYDKA NAITPTTSKP EFKGVSLNEA QKLARKKVYE ALKKSHEIFN KAESAYAFNT LIASCMEALN ALNAQNNERI LCEGYFVLLQ ILEPIIPHTA WELSERLFKR ENFKPIAIDE SALIEESMTL GLTINGKRRA ELKVNINASK EEIIVLAKKE LEKYLEKASV KKEIYVPNKL VNFVIA
NT Sequence
atggatttta tcaatataga aaaaaaatgg caagaatttt ggtggaaaaa taagagtttt gaacctaaag acgattttaa tctccctaaa aaatacattt taagcatgct gccttatcct agtggggaaa tccatatggg gcatgtgcgc aattacacca ttggcgatgc gttggcgcgt tattatcgtt tgcaccatta caatgtgtta caccctatgg ggtttgattc ttttgggatg cctgcagaaa atgcagccat taagcatggt atccacccta aaacctggac ttatgaaaac attgaagcca tgcaaaaaga gtttgaagct ttagggttct ctttttctaa aaacagggaa tttgccactt cagatccgga ttacacgaaa tttgaacagc aatttttcat tgatttgtgg gaaaaggggc taatttatcg caagaaagcc atgctcaatt ggtgtcctaa cgacaagacc gttttagcta atgagcaagt cattgatggg aggtgttggc gttgcgacac agaagttatc caaaaagaac tttatcaata ttatttgaag atcacaaact acgctgaaga attgctaaaa gacttagaga ctttagaaaa ccattggcct tctcaagtcc taatcatgca aaaaaactgg atagggaaat ctagcgggtt gcaatttggt tttaaaatcg ctgatgagtg cttgaaagct tgcaacggca ttcaagaaat tgaagttttt accacaagag cggatactat ttatggcgta acttacattg caatcgcccc agaacaccct ttagtagagc atgccattaa gcgagtgagc caagaagatt caaagatcat taaagcgatt ttaaacacga ctcaaagaga aagagcttta gagaaaaaag gggcgttttt aggcgtttat gctatccacc ctttaacgaa gcaaaaaatc cctgtttggg tggctaattt cgccctagcc aattatggct ctggggcgtt aatgggcgtg ccagcctgcg atgaaaggga ttttgaattc gctaatctgt atcatatccc tattaaagtg atcactcaaa gccctcaaaa tttgccccac accaaagaag aggttttaaa aaatagtggg gagtggagcg atctttctag ctcagtggcc agagaaaaaa ttatcgctta ttttgaaaaa gaaaacctcg gtaaaagggt gatcaactac cgcttgcaag attggggagt gagccgtcaa aggtattggg gagcgcccat tcctatgatt cattgcaaac attgtgggat tgtgcctgaa acccaactgc cggtaacttt acctgaagac attgtgattg atggggaagg caatccgtta gaaaagcatg cgagttggaa attcgctcaa tgccccaaat gccacaaaga cgctttaaga gaaacagaca ccatggatac tttcatccag tctagttggt atttcttgcg ctacaccacc cccaaaaatc agcgtgaaaa tcaagcgttt gatcaaaatt acttgaagta tttcatgcca gtggatactt atattggcgg cattgaacat gcgattttgc acttgttata cgcgcgcttt ttcactaagg ctttaaggga tttgggctat attcatttag atgagccttt caaacagctt atcactcaag gcatggtctt aaaaaatggc actaagatga gcaaatctaa aggtaatgtc gttagcccta aagaaatact caaaaaatac ggggccgatg ccgcaaggct ttttatcctt tttgctgccc caccggctaa agagttagaa tggaatgaca gcgctttaga gggcgcgcac cggtttatca agcgcttata cgataaagcg aacgccatta cccctaccac ttctaagcct gaatttaaag gagtcagcct gaatgaagcg caaaaactag ctcgtaaaaa agtctatgaa gcgttgaaaa aatcgcatga aattttcaat aaggctgaaa gcgcttacgc gtttaacact ttgatcgcaa gctgcatgga ggctttaaac gctttgaatg cgcaaaataa tgagcggatt ttatgcgagg gttattttgt gttgttgcaa attttagagc ctattatccc acacaccgca tgggagttga gcgagaggct ttttaaaaga gagaatttta agcctatagc gatcgatgaa agtgctttga tagaagaatc tatgacttta gggcttacca ttaatggcaa aaggcgcgcg gaattgaaag tcaatattaa cgccagtaaa gaagaaatta ttgttttggc taaaaaagaa ttagagaaat atttagaaaa ggcgagcgtt aaaaaagaaa tttatgtgcc taataagctc gttaattttg ttatcgcatg a
Details for HepyC.17943.a.B1.GE41185
HARVESTED ON: 3/1/2017
SEQUENCED ON: 3/2/2017
EXPECTED MW: 94kDa
OBSERVED MW: 94kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMD FINIEKKWQE FWWKNKSFEP KDDFNLPKKY ILSMLPYPSG EIHMGHVRNY TIGDALARYY RLHHYNVLHP MGFDSFGMPA ENAAIKHGIH PKTWTYENIE AMQKEFEALG FSFSKNREFA TSDPDYTKFE QQFFIDLWEK GLIYRKKAML NWCPNDKTVL ANEQVIDGRC WRCDTEVIQK ELYQYYLKIT NYAEELLKDL ETLENHWPSQ VLIMQKNWIG KSSGLQFGFK IADECLKACN GIQEIEVFTT RADTIYGVTY IAIAPEHPLV EHAIKRVSQE DSKIIKAILN TTQRERALEK KGAFLGVYAI HPLTKQKIPV WVANFALANY GSGALMGVPA CDERDFEFAN LYHIPIKVIT QSPQNLPHTK EEVLKNSGEW SDLSSSVARE KIIAYFEKEN LGKRVINYRL QDWGVSRQRY WGAPIPMIHC KHCGIVPETQ LPVTLPEDIV IDGEGNPLEK HASWKFAQCP KCHKDALRET DTMDTFIQSS WYFLRYTTPK NQRENQAFDQ NYLKYFMPVD TYIGGIEHAI LHLLYARFFT KALRDLGYIH LDEPFKQLIT QGMVLKNGTK MSKSKGNVVS PKEILKKYGA DAARLFILFA APPAKELEWN DSALEGAHRF IKRLYDKANA ITPTTSKPEF KGVSLNEAQK LARKKVYEAL KKSHEIFNKA ESAYAFNTLI ASCMEALNAL NAQNNERILC EGYFVLLQIL EPIIPHTAWE LSERLFKREN FKPIAIDESA LIEESMTLGL TINGKRRAEL KVNINASKEE IIVLAKKELE KYLEKASVKK EIYVPNKLVN FVIA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt ttatcaatat agaaaaaaaa tggcaagaat tttggtggaa aaataagagt tttgaaccta aagacgattt taatctccct aaaaaataca ttttaagcat gctgccttat cctagtgggg aaatccatat ggggcatgtg cgcaattaca ccattggcga tgcgttggcg cgttattatc gtttgcacca ttacaatgtg ttacacccta tggggtttga ttcttttggg atgcctgcag aaaatgcagc cattaagcat ggtatccacc ctaaaacctg gacttatgaa aacattgaag ccatgcaaaa agagtttgaa gctttagggt tctctttttc taaaaacagg gaatttgcca cttcagatcc ggattacacg aaatttgaac agcaattttt cattgatttg tgggaaaagg ggctaattta tcgcaagaaa gccatgctca attggtgtcc taacgacaag accgttttag ctaatgagca agtcattgat gggaggtgtt ggcgttgcga cacagaagtt atccaaaaag aactttatca atattatttg aagatcacaa actacgctga agaattgcta aaagacttag agactttaga aaaccattgg ccttctcaag tcctaatcat gcaaaaaaac tggataggga aatctagcgg gttgcaattt ggttttaaaa tcgctgatga gtgcttgaaa gcttgcaacg gcattcaaga aattgaagtt tttaccacaa gagcggatac tatttatggc gtaacttaca ttgcaatcgc cccagaacac cctttagtag agcatgccat taagcgagtg agccaagaag attcaaagat cattaaagcg attttaaaca cgactcaaag agaaagagct ttagagaaaa aaggggcgtt tttaggcgtt tatgctatcc accctttaac gaagcaaaaa atccctgttt gggtggctaa tttcgcccta gccaattatg gctctggggc gttaatgggc gtgccagcct gcgatgaaag ggattttgaa ttcgctaatc tgtatcatat ccctattaaa gtgatcactc aaagccctca aaatttgccc cacaccaaag aagaggtttt aaaaaatagt ggggagtgga gcgatctttc tagctcagtg gccagagaaa aaattatcgc ttattttgaa aaagaaaacc tcggtaaaag ggtgatcaac taccgcttgc aagattgggg agtgagccgt caaaggtatt ggggagcgcc cattcctatg attcattgca aacattgtgg gattgtgcct gaaacccaac tgccggtaac tttacctgaa gacattgtga ttgatgggga aggcaatccg ttagaaaagc atgcgagttg gaaattcgct caatgcccca aatgccacaa agacgcttta agagaaacag acaccatgga tactttcatc cagtctagtt ggtatttctt gcgctacacc acccccaaaa atcagcgtga aaatcaagcg tttgatcaaa attacttgaa gtatttcatg ccagtggata cttatattgg cggcattgaa catgcgattt tgcacttgtt atacgcgcgc tttttcacta aggctttaag ggatttgggc tatattcatt tagatgagcc tttcaaacag cttatcactc aaggcatggt cttaaaaaat ggcactaaga tgagcaaatc taaaggtaat gtcgttagcc ctaaagaaat actcaaaaaa tacggggccg atgccgcaag gctttttatc ctttttgctg ccccaccggc taaagagtta gaatggaatg acagcgcttt agagggcgcg caccggttta tcaagcgctt atacgataaa gcgaacgcca ttacccctac cacttctaag cctgaattta aaggagtcag cctgaatgaa gcgcaaaaac tagctcgtaa aaaagtctat gaagcgttga aaaaatcgca tgaaattttc aataaggctg aaagcgctta cgcgtttaac actttgatcg caagctgcat ggaggcttta aacgctttga atgcgcaaaa taatgagcgg attttatgcg agggttattt tgtgttgttg caaattttag agcctattat cccacacacc gcatgggagt tgagcgagag gctttttaaa agagagaatt ttaagcctat agcgatcgat gaaagtgctt tgatagaaga atctatgact ttagggctta ccattaatgg caaaaggcgc gcggaattga aagtcaatat taacgccagt aaagaagaaa ttattgtttt ggctaaaaaa gaattagaga aatatttaga aaaggcgagc gttaaaaaag aaatttatgt gcctaataag ctcgttaatt ttgttatcgc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for HepyC.17943.a.B1.PW38402
PURIFICATION DATe: 12/5/2017
CONCENTRATION: 17.72mg/ml
OBSERVED MW: 93kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
Two pellet purification LSEID 42966 and 43161. Added 5mM Leu and ATP to lysis buffer
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMD FINIEKKWQE FWWKNKSFEP KDDFNLPKKY ILSMLPYPSG EIHMGHVRNY TIGDALARYY RLHHYNVLHP MGFDSFGMPA ENAAIKHGIH PKTWTYENIE AMQKEFEALG FSFSKNREFA TSDPDYTKFE QQFFIDLWEK GLIYRKKAML NWCPNDKTVL ANEQVIDGRC WRCDTEVIQK ELYQYYLKIT NYAEELLKDL ETLENHWPSQ VLIMQKNWIG KSSGLQFGFK IADECLKACN GIQEIEVFTT RADTIYGVTY IAIAPEHPLV EHAIKRVSQE DSKIIKAILN TTQRERALEK KGAFLGVYAI HPLTKQKIPV WVANFALANY GSGALMGVPA CDERDFEFAN LYHIPIKVIT QSPQNLPHTK EEVLKNSGEW SDLSSSVARE KIIAYFEKEN LGKRVINYRL QDWGVSRQRY WGAPIPMIHC KHCGIVPETQ LPVTLPEDIV IDGEGNPLEK HASWKFAQCP KCHKDALRET DTMDTFIQSS WYFLRYTTPK NQRENQAFDQ NYLKYFMPVD TYIGGIEHAI LHLLYARFFT KALRDLGYIH LDEPFKQLIT QGMVLKNGTK MSKSKGNVVS PKEILKKYGA DAARLFILFA APPAKELEWN DSALEGAHRF IKRLYDKANA ITPTTSKPEF KGVSLNEAQK LARKKVYEAL KKSHEIFNKA ESAYAFNTLI ASCMEALNAL NAQNNERILC EGYFVLLQIL EPIIPHTAWE LSERLFKREN FKPIAIDESA LIEESMTLGL TINGKRRAEL KVNINASKEE IIVLAKKELE KYLEKASVKK EIYVPNKLVN FVIA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATT TTATCAATAT AGAAAAAAAA TGGCAAGAAT TTTGGTGGAA AAATAAGAGT TTTGAACCTA AAGACGATTT TAATCTCCCT AAAAAATACA TTTTAAGCAT GCTGCCTTAT CCTAGTGGGG AAATCCATAT GGGGCATGTG CGCAATTACA CCATTGGCGA TGCGTTGGCG CGTTATTATC GTTTGCACCA TTACAATGTG TTACACCCTA TGGGGTTTGA TTCTTTTGGG ATGCCTGCAG AAAATGCAGC CATTAAGCAT GGTATCCACC CTAAAACCTG GACTTATGAA AACATTGAAG CCATGCAAAA AGAGTTTGAA GCTTTAGGGT TCTCTTTTTC TAAAAACAGG GAATTTGCCA CTTCAGATCC GGATTACACG AAATTTGAAC AGCAATTTTT CATTGATTTG TGGGAAAAGG GGCTAATTTA TCGCAAGAAA GCCATGCTCA ATTGGTGTCC TAACGACAAG ACCGTTTTAG CTAATGAGCA AGTCATTGAT GGGAGGTGTT GGCGTTGCGA CACAGAAGTT ATCCAAAAAG AACTTTATCA ATATTATTTG AAGATCACAA ACTACGCTGA AGAATTGCTA AAAGACTTAG AGACTTTAGA AAACCATTGG CCTTCTCAAG TCCTAATCAT GCAAAAAAAC TGGATAGGGA AATCTAGCGG GTTGCAATTT GGTTTTAAAA TCGCTGATGA GTGCTTGAAA GCTTGCAACG GCATTCAAGA AATTGAAGTT TTTACCACAA GAGCGGATAC TATTTATGGC GTAACTTACA TTGCAATCGC CCCAGAACAC CCTTTAGTAG AGCATGCCAT TAAGCGAGTG AGCCAAGAAG ATTCAAAGAT CATTAAAGCG ATTTTAAACA CGACTCAAAG AGAAAGAGCT TTAGAGAAAA AAGGGGCGTT TTTAGGCGTT TATGCTATCC ACCCTTTAAC GAAGCAAAAA ATCCCTGTTT GGGTGGCTAA TTTCGCCCTA GCCAATTATG GCTCTGGGGC GTTAATGGGC GTGCCAGCCT GCGATGAAAG GGATTTTGAA TTCGCTAATC TGTATCATAT CCCTATTAAA GTGATCACTC AAAGCCCTCA AAATTTGCCC CACACCAAAG AAGAGGTTTT AAAAAATAGT GGGGAGTGGA GCGATCTTTC TAGCTCAGTG GCCAGAGAAA AAATTATCGC TTATTTTGAA AAAGAAAACC TCGGTAAAAG GGTGATCAAC TACCGCTTGC AAGATTGGGG AGTGAGCCGT CAAAGGTATT GGGGAGCGCC CATTCCTATG ATTCATTGCA AACATTGTGG GATTGTGCCT GAAACCCAAC TGCCGGTAAC TTTACCTGAA GACATTGTGA TTGATGGGGA AGGCAATCCG TTAGAAAAGC ATGCGAGTTG GAAATTCGCT CAATGCCCCA AATGCCACAA AGACGCTTTA AGAGAAACAG ACACCATGGA TACTTTCATC CAGTCTAGTT GGTATTTCTT GCGCTACACC ACCCCCAAAA ATCAGCGTGA AAATCAAGCG TTTGATCAAA ATTACTTGAA GTATTTCATG CCAGTGGATA CTTATATTGG CGGCATTGAA CATGCGATTT TGCACTTGTT ATACGCGCGC TTTTTCACTA AGGCTTTAAG GGATTTGGGC TATATTCATT TAGATGAGCC TTTCAAACAG CTTATCACTC AAGGCATGGT CTTAAAAAAT GGCACTAAGA TGAGCAAATC TAAAGGTAAT GTCGTTAGCC CTAAAGAAAT ACTCAAAAAA TACGGGGCCG ATGCCGCAAG GCTTTTTATC CTTTTTGCTG CCCCACCGGC TAAAGAGTTA GAATGGAATG ACAGCGCTTT AGAGGGCGCG CACCGGTTTA TCAAGCGCTT ATACGATAAA GCGAACGCCA TTACCCCTAC CACTTCTAAG CCTGAATTTA AAGGAGTCAG CCTGAATGAA GCGCAAAAAC TAGCTCGTAA AAAAGTCTAT GAAGCGTTGA AAAAATCGCA TGAAATTTTC AATAAGGCTG AAAGCGCTTA CGCGTTTAAC ACTTTGATCG CAAGCTGCAT GGAGGCTTTA AACGCTTTGA ATGCGCAAAA TAATGAGCGG ATTTTATGCG AGGGTTATTT TGTGTTGTTG CAAATTTTAG AGCCTATTAT CCCACACACC GCATGGGAGT TGAGCGAGAG GCTTTTTAAA AGAGAGAATT TTAAGCCTAT AGCGATCGAT GAAAGTGCTT TGATAGAAGA ATCTATGACT TTAGGGCTTA CCATTAATGG CAAAAGGCGC GCGGAATTGA AAGTCAATAT TAACGCCAGT AAAGAAGAAA TTATTGTTTT GGCTAAAAAA GAATTAGAGA AATATTTAGA AAAGGCGAGC GTTAAAAAAG AAATTTATGT GCCTAATAAG CTCGTTAATT TTGTTATCGC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT