SSGCID: Target Page

HepyC.17938.a

UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) (D-glutamic acid-adding enzyme) (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase)

CENTER ID:	HepyC.17938.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.17938.a.B2.GE41114	soluble domain #1		1	237

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.492
UniProt:	B5Z6L9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKISLLGHGK TTLALARFFK KNHNEVKFFD DQFTAFHKDE EGFLCYPSKD FNPNDSQLEV VSPGISFMHP LVIKAKHLVS EYDYINSLFD LVFTPTIISI SGTNGKTTTT EMLTALLEDF KAVSGGNIGT PLIELFEKQS PLWVLETSSF SLHYTNKAYP LIYLLINVEA DHLTWHCNFE NYLNAKLKVL TLMPKTSLAI LPLKFKEHPI IQNSQAQKIF FDHSEEVLER LKIPSNALFF KGAFLLDAAL ALLVYEQFLK IKNLKWQDYR ENALKRLNAF KIGSHKMEEF RDKEGRLWVD DSKATNIDAT LQALKTFKNQ KIHLILGGDI KGVNLTPLFE EFKNYKISLY AIGSSASIIQ ALALEFNISC QVCLKLEKAV QEIKSVLLQN EIALLSPSAA SLDQFSSYKE RGEKFKAFVL KD

NT Sequence

atgaaaattt ctttattggg gcatggtaaa accactctag ccctagcgcg tttttttaaa aaaaaccata acgaagtcaa attttttgat gatcaattca ctgcatttca taaggatgaa gagggttttc tttgctaccc tagtaaggat tttaacccta atgattccca actagaggta gtcagccctg gcattagttt catgcaccct ttagtcataa aagccaagca tttagtgagc gaatacgatt atattaatag cttgtttgat ttggttttca cgcctactat aatcagtatt agcggcacta acgggaaaac caccacgacc gaaatgctca ctgcgctttt agaagatttt aaggctgtga gtggggggaa tatcggcacg cccttgattg aattgtttga aaagcaatcg cccttgtggg tgttagaaac aagctccttt tctttgcatt acacgaataa ggcttaccct ttaatctact tgcttatcaa tgtggaagct gatcatttaa cctggcattg caattttgaa aattatttga acgctaaact caaggttcta acattgatgc ctaaaacttc gctcgctatc ctccctttaa aattcaaaga acaccccatt attcaaaact cacaagcgca aaaaatcttt tttgatcaca gcgaagaagt tttagaacgt ttaaaaatcc cttctaacgc tctttttttt aagggagcgt ttttattgga cgctgcctta gcccttttag tttatgagca atttttaaaa ataaagaatt taaaatggca agattatcga gaaaacgccc ttaaaagatt aaacgctttt aaaattggct cgcataaaat ggaagaattt agggataaag aagggcgttt gtgggtagat gacagcaagg ccacgaatat tgatgccacc ttgcaagccc taaaaacctt taaaaaccaa aaaatccatt tgattttagg gggcgatatt aaaggggtca atttaacccc cctttttgaa gagtttaaaa actataaaat aagcctttat gccataggat caagcgcttc tatcatccaa gctttagcgt tagaatttaa tatttcttgt caggtttgtt taaagttaga aaaagcggtt caagaaatta aaagcgtttt attgcaaaat gaaattgctt tgctttcacc cagtgcggcc agtttggatc aattttcttc gtataaagaa aggggtgaaa aatttaaagc gtttgtttta aaggattaa

Details for HepyC.17938.a.B2.GE41114

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK ISLLGHGKTT LALARFFKKN HNEVKFFDDQ FTAFHKDEEG FLCYPSKDFN PNDSQLEVVS PGISFMHPLV IKAKHLVSEY DYINSLFDLV FTPTIISISG TNGKTTTTEM LTALLEDFKA VSGGNIGTPL IELFEKQSPL WVLETSSFSL HYTNKAYPLI YLLINVEADH LTWHCNFENY LNAKLKVLTL MPKTSLAILP LKFKEHPIIQ NSQAQKIFFD HSEEVLERLK IPSNA

Validated NT Sequence

atcttactca cttaagcgtt agaagggatt tttaaacgtt ctaaaacttc ttcgctgtga tcaaaaaaga ttttttgcgc ttgtgagttt tgaataatgg ggtgttcttt gaattttaaa gggaggatag cgagcgaagt tttaggcatc aatgttagaa ccttgagttt agcgttcaaa taattttcaa aattgcaatg ccaggttaaa tgatcagctt ccacattgat aagcaagtag attaaagggt aagccttatt cgtgtaatgc aaagaaaagg agcttgtttc taacacccac aagggcgatt gcttttcaaa caattcaatc aagggcgtgc cgatattccc cccactcaca gccttaaaat cttctaaaag cgcagtgagc atttcggtcg tggtggtttt cccgttagtg ccgctaatac tgattatagt aggcgtgaaa accaaatcaa acaagctatt aatataatcg tattcgctca ctaaatgctt ggcttttatg actaaagggt gcatgaaact aatgccaggg ctgactacct ctagttggga atcattaggg ttaaaatcct tactagggta gcaaagaaaa ccctcttcat ccttatgaaa tgcagtgaat tgatcatcaa aaaatttgac ttcgttatgg ttttttttaa aaaaacgcgc tagggctaga gtggttttac catgccccaa taaagaaatt ttcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tttctttatt ggggcatggt aaaaccactc tagccctagc gcgttttttt aaaaaaaacc ataacgaagt caaatttttt gatgatcaat tcactgcatt tcataaggat gaagagggtt ttctttgcta ccctagtaag gattttaacc ctaatgattc ccaactagag gtagtcagcc ctggcattag tttcatgcac cctttagtca taaaagccaa gcatttagtg agcgaatacg attatattaa tagcttgttt gatttggttt tcacgcctac tataatcagt attagcggca ctaacgggaa aaccaccacg accgaaatgc tcactgcgct tttagaagat tttaaggctg tgagtggggg gaatatcggc acgcccttga ttgaattgtt tgaaaagcaa tcgcccttgt gggtgttaga aacaagctcc ttttctttgc attacacgaa taaggcttac cctttaatct acttgcttat caatgtggaa gctgatcatt taacctggca ttgcaatttt gaaaattatt tgaacgctaa actcaaggtt ctaacattga tgcctaaaac ttcgctcgct atcctccctt taaaattcaa agaacacccc attattcaaa actcacaagc gcaaaaaatc ttttttgatc acagcgaaga agttttagaa cgtttaaaaa tcccttctaa cgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat