HepyC.17928.a

Glycine--tRNA ligase beta subunit (EC 6.1.1.14) (Glycyl-tRNA synthetase beta subunit) (GlyRS)

CENTER ID: HepyC.17928.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17928.a.B1.GE41110 full length 1 701
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17928.a.B1.PW38376 full length 1 701

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.995
UniProt: B5Z7X4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHSDELLVEI LVEELPAQAL LNEYKEMPKK LHALFNKRAL EVGNIEIFYT PRRLCLLIKD FPLLTQETKE EFFGPPVKIA CNNEDKTQGL NALGLGFYQK LGLKDHQHFQ TAFKNNKEVL YHAKIHEKEP TKDLIMPIVL EFLEDLNFGK SMRWGNVEKS FIRPIHNICV LFNGENFNDI EVKEYGFKTK QATKVHRQES FDFIEVDSPK AYFEVLEKNH VILDPKKREA KILQEIKELE TEHHISIEID RDLLDEVVAI TEYPSALLGE FDKAFLKLPS EIITTSMKEN QRYFATFCQK SQEESPTLHN GFIVVSNAIN KDKQKIILGN QKVLKARLSD AVFFYENDLK KPLDNAPLES VVFVQGLGTL KDKMERESII AQYLTQKYAP SLNMPLEKAL ELVKRAVQIA KADLLSEVVY EFSELQGIMG YYYALKQNEN ELVALSLKEQ YLPASENAPL PSSVFSAIVA LSLKLDSLFS LFSVGKIPSG SKDPFALRRL SFGLLKIIAH YGLEFDLKAD LKSLFEKVGV YQSFDLEILE KFLLERFNNL IDCNPSIIRS VLNTNERDIV KIIQKVKALK RFLDDPKNAQ KKELLFSAFK RLANINKDRN PNESSEFSIS LFKESQEHAL FEAFNAIKTS AFEGLDSKIE AYFGLHAPLE EYFKSVLVMD KDIEIQKNRK NFLWSVYQSF LEIGDIKEIA I
NT Sequence
ttgcattcag atgaattgtt agtagagatt ttagttgaag aattgcccgc gcaagcgtta ttgaatgaat ataaagaaat gcctaaaaaa ctccatgctc tttttaataa gcgcgcttta gaagtgggaa atatagagat tttttacacc cctaggcgtt tgtgtttgct cattaaagat tttcctcttt taacccaaga aaccaaagag gaattttttg ggcctcccgt taaaatcgca tgcaacaatg aagataaaac gcaagggttg aacgcactag gtttagggtt ttatcaaaaa ttagggttaa aagatcacca acatttccaa acagcgttta aaaacaataa agaagtgctt tatcacgcta aaatccatga aaaagagcct acaaaagact taatcatgcc cattgtgtta gagtttttag aggatttgaa tttcgggaag tctatgcgtt ggggcaatgt ggaaaaaagc tttatcagac ccattcataa tatttgcgtg ttgtttaatg gggaaaattt taacgacatt gaagttaaag agtatggttt taaaaccaag caagccacga aagtgcaccg acaagagagt tttgatttta ttgaagtgga tagccccaaa gcgtattttg aagttttaga aaaaaaccat gtcattctag accctaaaaa gcgcgaagct aaaatcttac aagaaattaa agagctagaa acagagcatc acattagcat agaaatagat agggatttat tagatgaggt tgtagcgatc acggaatacc ccagcgcgct tttaggggag tttgacaagg cgtttttaaa attacccagt gaaatcatca ccacttccat gaaagaaaac caacgctatt ttgcaacctt ttgtcaaaaa agccaagaag aaagcccaac attacacaac ggctttattg tggtgagtaa cgctatcaat aaagacaagc aaaaaatcat tttaggcaat caaaaggttt taaaagcccg cttgagcgat gcggttttct tttatgaaaa cgatctcaaa aagcctttag ataacgcccc tttagagagc gtggtttttg tgcaaggttt agggacttta aaagataaaa tggagcgaga atcaatcatc gcccaatatt tgacgcaaaa atacgctcca tctttaaaca tgcctttaga aaaagccctt gagttggtta aaagagccgt tcaaatcgct aaggcggatt tactcagtga agtggtgtat gaatttagcg agcttcaagg gatcatgggc tattactacg ctttgaaaca aaacgaaaac gaattggtcg ccttgagctt gaaagagcag tatttgcccg caagcgaaaa cgctcccttg ccctctagcg tttttagtgc gatcgtggct ttgagcttga aattagacag cctgttttct ctttttagcg ttggtaaaat ccctagcgga tctaaagatc cttttgcttt aaggcgtttg agttttgggc tattgaaaat catcgcgcat tacgggttag aatttgattt gaaagcggat ttgaaaagcc tctttgaaaa agtgggtgtt tatcaaagct ttgatttaga gattttagag aagtttttac tggagcgctt taataattta atagattgta acccctctat tataaggagc gtgttaaaca ccaatgagcg agatattgtt aaaatcattc aaaaagtcaa agccttaaaa cgctttttag acgatcccaa gaacgctcaa aaaaaagagt tgctttttag cgctttcaaa cgcctagcca atatcaataa agacagaaac cctaacgaat caagcgagtt ttctataagt cttttcaaag aatcgcaaga gcatgccctt tttgaagcgt tcaatgcgat caaaacaagc gcttttgagg gtttggatag caaaatagag gcttattttg gtttgcatgc gcctttagaa gagtatttta aaagcgtgct ggtgatggat aaagacatag aaatccaaaa aaatcgtaaa aatttcttgt ggagtgtgta tcaaagtttc ttagaaattg gggatattaa agaaattgcg atttaa
Details for HepyC.17928.a.B1.GE41110
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 82kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMH SDELLVEILV EELPAQALLN EYKEMPKKLH ALFNKRALEV GNIEIFYTPR RLCLLIKDFP LLTQETKEEF FGPPVKIACN NEDKTQGLNA LGLGFYQKLG LKDHQHFQTA FKNNKEVLYH AKIHEKEPTK DLIMPIVLEF LEDLNFGKSM RWGNVEKSFI RPIHNICVLF NGENFNDIEV KEYGFKTKQA TKVHRQESFD FIEVDSPKAY FEVLEKNHVI LDPKKREAKI LQEIKELETE HHISIEIDRD LLDEVVAITE YPSALLGEFD KAFLKLPSEI ITTSMKENQR YFATFCQKSQ EESPTLHNGF IVVSNAINKD KQKIILGNQK VLKARLSDAV FFYENDLKKP LDNAPLESVV FVQGLGTLKD KMERESIIAQ YLTQKYAPSL NMPLEKALEL VKRAVQIAKA DLLSEVVYEF SELQGIMGYY YALKQNENEL VALSLKEQYL PASENAPLPS SVFSAIVALS LKLDSLFSLF SVGKIPSGSK DPFALRRLSF GLLKIIAHYG LEFDLKADLK SLFEKVGVYQ SFDLEILEKF LLERFNNLID CNPSIIRSVL NTNERDIVKI IQKVKALKRF LDDPKNAQKK ELLFSAFKRL ANINKDRNPN ESSEFSISLF KESQEHALFE AFNAIKTSAF EGLDSKIEAY FGLHAPLEEY FKSVLVMDKD IEIQKNRKNF LWSVYQSFLE IGDIKEIAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcatt cagatgaatt gttagtagag attttagttg aagaattgcc cgcgcaagcg ttattgaatg aatataaaga aatgcctaaa aaactccatg ctctttttaa taagcgcgct ttagaagtgg gaaatataga gattttttac acccctaggc gtttgtgttt gctcattaaa gattttcctc ttttaaccca agaaaccaaa gaggaatttt ttgggcctcc cgttaaaatc gcatgcaaca atgaagataa aacgcaaggg ttgaacgcac taggtttagg gttttatcaa aaattagggt taaaagatca ccaacatttc caaacagcgt ttaaaaacaa taaagaagtg ctttatcacg ctaaaatcca tgaaaaagag cctacaaaag acttaatcat gcccattgtg ttagagtttt tagaggattt gaatttcggg aagtctatgc gttggggcaa tgtggaaaaa agctttatca gacccattca taatatttgc gtgttgttta atggggaaaa ttttaacgac attgaagtta aagagtatgg ttttaaaacc aagcaagcca cgaaagtgca ccgacaagag agttttgatt ttattgaagt ggatagcccc aaagcgtatt ttgaagtttt agaaaaaaac catgtcattc tagaccctaa aaagcgcgaa gctaaaatct tacaagaaat taaagagcta gaaacagagc atcacattag catagaaata gatagggatt tattagatga ggttgtagcg atcacggaat accccagcgc gcttttaggg gagtttgaca aggcgttttt aaaattaccc agtgaaatca tcaccacttc catgaaagaa aaccaacgct attttgcaac cttttgtcaa aaaagccaag aagaaagccc aacattacac aacggcttta ttgtggtgag taacgctatc aataaagaca agcaaaaaat cattttaggc aatcaaaagg ttttaaaagc ccgcttgagc gatgcggttt tcttttatga aaacgatctc aaaaagcctt tagataacgc ccctttagag agcgtggttt ttgtgcaagg tttagggact ttaaaagata aaatggagcg agaatcaatc atcgcccaat atttgacgca aaaatacgct ccatctttaa acatgccttt agaaaaagcc cttgagttgg ttaaaagagc cgttcaaatc gctaaggcgg atttactcag tgaagtggtg tatgaattta gcgagcttca agggatcatg ggctattact acgctttgaa acaaaacgaa aacgaattgg tcgccttgag cttgaaagag cagtatttgc ccgcaagcga aaacgctccc ttgccctcta gcgtttttag tgcgatcgtg gctttgagct tgaaattaga cagcctgttt tctcttttta gcgttggtaa aatccctagc ggatctaaag atccttttgc tttaaggcgt ttgagttttg ggctattgaa aatcatcgcg cattacgggt tagaatttga tttgaaagcg gatttgaaaa gcctctttga aaaagtgggt gtttatcaaa gctttgattt agagatttta gagaagtttt tactggagcg ctttaataat ttaatagatt gtaacccctc tattataagg agcgtgttaa acaccaatga gcgagatatt gttaaaatca ttcaaaaagt caaagcctta aaacgctttt tagacgatcc caagaacgct caaaaaaaag agttgctttt tagcgctttc aaacgcctag ccaatatcaa taaagacaga aaccctaacg aatcaagcga gttttctata agtcttttca aagaatcgca agagcatgcc ctttttgaag cgttcaatgc gatcaaaaca agcgcttttg agggtttgga tagcaaaata gaggcttatt ttggtttgca tgcgccttta gaagagtatt ttaaaagcgt gctggtgatg gataaagaca tagaaatcca aaaaaatcgt aaaaatttct tgtggagtgt gtatcaaagt ttcttagaaa ttggggatat taaagaaatt gcgatttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for HepyC.17928.a.B1.PW38376
PURIFICATION DATe: 10/23/2017
CONCENTRATION: 25mg/ml
OBSERVED MW: 76kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
Two pellet combined purification IMAC->SEC
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMH SDELLVEILV EELPAQALLN EYKEMPKKLH ALFNKRALEV GNIEIFYTPR RLCLLIKDFP LLTQETKEEF FGPPVKIACN NEDKTQGLNA LGLGFYQKLG LKDHQHFQTA FKNNKEVLYH AKIHEKEPTK DLIMPIVLEF LEDLNFGKSM RWGNVEKSFI RPIHNICVLF NGENFNDIEV KEYGFKTKQA TKVHRQESFD FIEVDSPKAY FEVLEKNHVI LDPKKREAKI LQEIKELETE HHISIEIDRD LLDEVVAITE YPSALLGEFD KAFLKLPSEI ITTSMKENQR YFATFCQKSQ EESPTLHNGF IVVSNAINKD KQKIILGNQK VLKARLSDAV FFYENDLKKP LDNAPLESVV FVQGLGTLKD KMERESIIAQ YLTQKYAPSL NMPLEKALEL VKRAVQIAKA DLLSEVVYEF SELQGIMGYY YALKQNENEL VALSLKEQYL PASENAPLPS SVFSAIVALS LKLDSLFSLF SVGKIPSGSK DPFALRRLSF GLLKIIAHYG LEFDLKADLK SLFEKVGVYQ SFDLEILEKF LLERFNNLID CNPSIIRSVL NTNERDIVKI IQKVKALKRF LDDPKNAQKK ELLFSAFKRL ANINKDRNPN ESSEFSISLF KESQEHALFE AFNAIKTSAF EGLDSKIEAY FGLHAPLEEY FKSVLVMDKD IEIQKNRKNF LWSVYQSFLE IGDIKEIAI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGCATT CAGATGAATT GTTAGTAGAG ATTTTAGTTG AAGAATTGCC CGCGCAAGCG TTATTGAATG AATATAAAGA AATGCCTAAA AAACTCCATG CTCTTTTTAA TAAGCGCGCT TTAGAAGTGG GAAATATAGA GATTTTTTAC ACCCCTAGGC GTTTGTGTTT GCTCATTAAA GATTTTCCTC TTTTAACCCA AGAAACCAAA GAGGAATTTT TTGGGCCTCC CGTTAAAATC GCATGCAACA ATGAAGATAA AACGCAAGGG TTGAACGCAC TAGGTTTAGG GTTTTATCAA AAATTAGGGT TAAAAGATCA CCAACATTTC CAAACAGCGT TTAAAAACAA TAAAGAAGTG CTTTATCACG CTAAAATCCA TGAAAAAGAG CCTACAAAAG ACTTAATCAT GCCCATTGTG TTAGAGTTTT TAGAGGATTT GAATTTCGGG AAGTCTATGC GTTGGGGCAA TGTGGAAAAA AGCTTTATCA GACCCATTCA TAATATTTGC GTGTTGTTTA ATGGGGAAAA TTTTAACGAC ATTGAAGTTA AAGAGTATGG TTTTAAAACC AAGCAAGCCA CGAAAGTGCA CCGACAAGAG AGTTTTGATT TTATTGAAGT GGATAGCCCC AAAGCGTATT TTGAAGTTTT AGAAAAAAAC CATGTCATTC TAGACCCTAA AAAGCGCGAA GCTAAAATCT TACAAGAAAT TAAAGAGCTA GAAACAGAGC ATCACATTAG CATAGAAATA GATAGGGATT TATTAGATGA GGTTGTAGCG ATCACGGAAT ACCCCAGCGC GCTTTTAGGG GAGTTTGACA AGGCGTTTTT AAAATTACCC AGTGAAATCA TCACCACTTC CATGAAAGAA AACCAACGCT ATTTTGCAAC CTTTTGTCAA AAAAGCCAAG AAGAAAGCCC AACATTACAC AACGGCTTTA TTGTGGTGAG TAACGCTATC AATAAAGACA AGCAAAAAAT CATTTTAGGC AATCAAAAGG TTTTAAAAGC CCGCTTGAGC GATGCGGTTT TCTTTTATGA AAACGATCTC AAAAAGCCTT TAGATAACGC CCCTTTAGAG AGCGTGGTTT TTGTGCAAGG TTTAGGGACT TTAAAAGATA AAATGGAGCG AGAATCAATC ATCGCCCAAT ATTTGACGCA AAAATACGCT CCATCTTTAA ACATGCCTTT AGAAAAAGCC CTTGAGTTGG TTAAAAGAGC CGTTCAAATC GCTAAGGCGG ATTTACTCAG TGAAGTGGTG TATGAATTTA GCGAGCTTCA AGGGATCATG GGCTATTACT ACGCTTTGAA ACAAAACGAA AACGAATTGG TCGCCTTGAG CTTGAAAGAG CAGTATTTGC CCGCAAGCGA AAACGCTCCC TTGCCCTCTA GCGTTTTTAG TGCGATCGTG GCTTTGAGCT TGAAATTAGA CAGCCTGTTT TCTCTTTTTA GCGTTGGTAA AATCCCTAGC GGATCTAAAG ATCCTTTTGC TTTAAGGCGT TTGAGTTTTG GGCTATTGAA AATCATCGCG CATTACGGGT TAGAATTTGA TTTGAAAGCG GATTTGAAAA GCCTCTTTGA AAAAGTGGGT GTTTATCAAA GCTTTGATTT AGAGATTTTA GAGAAGTTTT TACTGGAGCG CTTTAATAAT TTAATAGATT GTAACCCCTC TATTATAAGG AGCGTGTTAA ACACCAATGA GCGAGATATT GTTAAAATCA TTCAAAAAGT CAAAGCCTTA AAACGCTTTT TAGACGATCC CAAGAACGCT CAAAAAAAAG AGTTGCTTTT TAGCGCTTTC AAACGCCTAG CCAATATCAA TAAAGACAGA AACCCTAACG AATCAAGCGA GTTTTCTATA AGTCTTTTCA AAGAATCGCA AGAGCATGCC CTTTTTGAAG CGTTCAATGC GATCAAAACA AGCGCTTTTG AGGGTTTGGA TAGCAAAATA GAGGCTTATT TTGGTTTGCA TGCGCCTTTA GAAGAGTATT TTAAAAGCGT GCTGGTGATG GATAAAGACA TAGAAATCCA AAAAAATCGT AAAAATTTCT TGTGGAGTGT GTATCAAAGT TTCTTAGAAA TTGGGGATAT TAAAGAAATT GCGATTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T