HepyC.17926.a

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) (D-fructose-6-phosphate amidotransferase) (GFAT) (Glucosamine-6-phosphate synthase) (Hexosephosphate aminotransferase) (L-glutamine--D-fructose-6-phosphate amidotransferase)

CENTER ID: HepyC.17926.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17926.a.B1.GE41109 full length 1 597
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1579
UniProt: B5Z9E6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCGIVGYIGD SEKKSVLLEG LKELEYRGYD SAGLAVLSND RLEVFKTQGK LENLKSELKN KEFLDFGVSI AHTRWATHGK PSSANAHPHF TENLALVHNG IIENYASLKK ELENKGHAFL SQTDTEVIAH LLEETLKSES DLLKAFEKSI SLLKGSYAIL MLHKRAKESL FYAKSSSPLV VGKGKEGVFF ASSLSVLAPK VDQFVILEEN SVGRISLENF KDLNNIENMK DYAFEDKDYS KGNFRNYLEK EIYEQHSSLL ECLEGRLEAL NVYCEIDPEF LENVNEITLC SCGSSYHASL ASVYLFERLA KIRARAILAS EYRYAHFKSN PNELFIAISQ SGETADTLEA LKLAKAQGLK TISLCNAPFS MMSRISDHTL LIRAGVERSV ASTKAFSSQV MLLWLLSVYI GKQLGTISKE EERIQAKNML NSVKAMKVEP KLHEKIKRLS KRYLHGHGFF YIGRDVFYPL ALEGALKLKE ISYLHAEGYA SAEMKHGPIA LVDSNLFTIA LLSKHLLFDK TKSNIEELSA RDSTICVLSS EILEIADDFI QLEESESYME EFFRMNLAVQ LLALEIAMRL NHDVDHPRNL AKSVTVE
NT Sequence
atgtgtggga ttgtaggtta tataggggat agcgagaaaa aatccgttct tttagagggc ttaaaggaat tagaatacag aggctatgac agcgcgggtt tagccgtatt gagcaatgat cgtttggaag tgtttaaaac tcaagggaaa ttagaaaacc ttaaatcaga gcttaaaaat aaagagtttt tggattttgg cgtgagtatc gctcacacca gatgggcaac gcatggcaag ccaagcagcg cgaacgccca cccgcatttt acagaaaatt tagccttagt gcataatggc atcattgaaa attacgcgag tttgaaaaag gaattggaaa acaaagggca tgcgtttttg agccaaacgg acacggaagt gattgcacat ttattagaag aaacgcttaa aagcgagagc gatttattga aagcttttga aaaaagcatc agccttttaa aagggagtta tgcgatttta atgctccata aaagggctaa agagagctta ttttacgcta aatcttcttc gcctttagtt gtgggtaagg gcaaagaagg ggtgtttttt gcgtccagtt tgagcgtgtt agcccctaaa gtggatcaat ttgtcatttt agaagaaaac agcgtggggc ggatttcttt agaaaatttt aaagatttaa acaatattga aaacatgaaa gattacgctt ttgaggataa ggattattct aaaggcaatt ttaggaatta tttagaaaaa gagatttatg agcagcatag cagtttgtta gagtgtttag aggggcgctt ggaagccttg aatgtgtatt gcgagatcga tcctgaattt ttagagaatg tgaatgaaat cacgctgtgt tcttgcggga gcagttacca tgcgagtttg gcgagcgtgt atttgtttga aaggttagcc aaaataagag cgagagccat tttagcgagc gaataccgct acgcccattt taaaagcaac cctaacgagc tttttatagc gatttctcaa agcggcgaaa ccgctgatac tttggaggct ttaaaattag ccaaagccca agggcttaaa accattagct tgtgtaacgc cccttttagc atgatgagcc gcatcagcga tcacacgctt ttgattagag cgggggtaga aaggagcgtg gcatccacta aggcgttttc ttcgcaagtg atgcttttat ggcttttgag cgtgtatata ggcaaacagc tagggaccat ctctaaagaa gaagaaagaa tccaggctaa aaacatgctc aatagcgtga aggcgatgaa agtagagcct aaattgcatg aaaagatcaa gcgtttatcc aaacgctact tgcatgggca tggctttttt tatatcggcc gcgatgtgtt ttacccgctc gctttagaag gggcgctaaa acttaaagaa atcagctact tgcacgctga ggggtatgcg agcgcggaga tgaagcatgg acctattgcg ttagtggatt ctaacctttt taccattgct ttattgtcta agcacttgtt atttgataaa accaaaagca atattgaaga attgagcgct agggattcta cgatttgcgt gttaagctct gaaattttag aaattgctga tgattttatc caattagaag agagcgaaag ctacatggaa gaatttttcc gcatgaattt agcggtgcag cttttagcct tagaaatcgc tatgcgttta aatcatgatg tggatcaccc aagaaactta gctaaaagcg ttaccgtgga ataa
Details for HepyC.17926.a.B1.GE41109
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMC GIVGYIGDSE KKSVLLEGLK ELEYRGYDSA GLAVLSNDRL EVFKTQGKLE NLKSELKNKE FLDFGVSIAH TRWATHGKPS SANAHPHFTE NLALVHNGII ENYASLKKEL ENKGHAFLSQ TDTEVIAHLL EETLKSESDL LKAFEKSISL LKGSYAILML HKRAKESLFY AKSSSPLVVG KGKEGVFFAS SLSVLAPKVD QFVILEENSV GRISLENFKD LNNIENMKDY AFEDKXYSKG NFRNYLEKEI XXQHXSLLEX XXXRLXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXLXXXXAKI RXXXXXXXXX XXAHFKSNPN ELFIAISQSX ETADTLEALK LAKAQGLKTI SLCNAPFSMM SRISDHTLLI RAGVERSVAS TKAFSSQVML LWLLSVYIGK QLGTISKEEE RIQAKNMLNS VKAMKVEPKL HEKIKRLSKR YLHGHGFFYI GRDVFYPLAL EGALKLKEIS YLHAEGYASA EMKHGPIALV DSNLFTIALL SKHLLFDKTK SNIEELSARD STICVLSSEI LEIADDFIQL EESESYMEEF FRMNLAVQLL ALEIAMRLNH DVDHPRN
Validated NT Sequence
aagtttcttg ggtgatccac atcatgattt aaacgcatag cgatttctaa ggctaaaagc tgcaccgcta aattcatgcg gaaaaattct tccatgtagc tttcgctctc ttctaattgg ataaaatcat cagcaatttc taaaatttca gagcttaaca cgcaaatcgt agaatcccta gcgctcaatt cttcaatatt gcttttggtt ttatcaaata acaagtgctt agacaataaa gcaatggtaa aaaggttaga atccactaac gcaataggtc catgcttcat ctccgcgctc gcatacccct cagcgtgcaa gtagctgatt tctttaagtt ttagcgcccc ttctaaagcg agcgggtaaa acacatcgcg gccgatataa aaaaagccat gcccatgcaa gtagcgtttg gataaacgct tgatcttttc atgcaattta ggctctactt tcatcgcctt cacgctattg agcatgtttt tagcctggat tctttcttct tctttagaga tggtccctag ctgtttgcct atatacacgc tcaaaagcca taaaagcatc acttgcgaag aaaacgcctt agtggatgcc acgctccttt ctacccccgc tctaatcaaa agcgtgtgat cgctgatgcg gctcatcatg ctaaaagggg cgttacacaa gctaatggtt ttaagccctt gggctttggc taattttaaa gcctccaaag tatcagcggt ttcgncgctt tgagaaatcg ctataaaaag ctcgttaggg ttgcttttaa aatgggcgtn nnnnnnnnnn nnngnnnnnn nnnnnctnnn tcttattttg gcnnnnnnnn nnaacaaann nnnnnnnnnn nnnnnnnnnn nnnnnctgnn nncnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcagnan cnnnnnnnnn nnncnnnnnn nnnnnnnnca agcgccnnnn nnnncactct aacaaactnn natgctgctn ntaaatctct ttttctaaat aattcctaaa attgccttta gaatantcct tatcctcaaa agcgtaatct ttcatgtttt caatattgtt taaatcttta aaattttcta aagaaatccg ccccacgctg ttttcttcta aaatgacaaa ttgatccact ttaggggcta acacgctcaa actggacgca aaaaacaccc cttctttgcc cttacccaca actaaaggcg aagaagattt agcgtaaaat aagctctctt tagccctttt atggagcatt aaaatcgcat aactcccttt taaaaggctg atgctttttt caaaagcttt caataaatcg ctctcgcttt taagcgtttc ttctaataaa tgtgcaatca cttccgtgtc cgtttggctc aaaaacgcat gccctttgtt ttccaattcc tttttcaaac tcgcgtaatt ttcaatgatg ccattatgca ctaaggctaa attttctgta aaatgcgggt gggcgttcgc gctgcttggc ttgccatgcg ttgcccatct ggtgtgagcg atactcacgc caaaatccaa aaactcttta tttttaagct ctgatttaag gttttctaat ttcccttgag ttttaaacac ttccaaacga tcattgctca atacggctaa acccgcgctg tcatagcctc tgtattctaa ttcctttaag ccctctaaaa gaacggattt tttctcgcta tcccctatat aacctacaat cccacacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMC GIVGYIGDSE KKSVLLEGLK ELEYRGYDSA GLAVLSNDRL EVFKTQGKLE NLKSELKNKE FLDFGVSIAH TRWATHGKPS SANAHPHFTE NLALVHNGII ENYASLKKEL ENKGHAFLSQ TDTEVIAHLL EETLKSESDL LKAFEKSISL LKGSYAILML HKRAKESLFY AKSSSPLVVG KGKEGVFFAS SLSVLAPKVD QFVILEENSV GRISLENFKD LNNIENMKDY AFEDKDYSKG NFRNYLEKEI YEQHSSLLEC LEGRLEALNV YCEIDPEFLE NVNEITLCSC GSSYHASLAS VYLFERLAKI RARAILASEY RYAHFKSNPN ELFIAISQSG ETADTLEALK LAKAQGLKTI SLCNAPFSMM SRISDHTLLI RAGVERSVAS TKAFSSQVML LWLLSVYIGK QLGTISKEEE RIQAKNMLNS VKAMKVEPKL HEKIKRLSKR YLHGHGFFYI GRDVFYPLAL EGALKLKEIS YLHAEGYASA EMKHGPIALV DSNLFTIALL SKHLLFDKTK SNIEELSARD STICVLSSEI LEIADDFIQL EESESYMEEF FRMNLAVQLL ALEIAMRLNH DVDHPRNLAK SVTVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtg ggattgtagg ttatataggg gatagcgaga aaaaatccgt tcttttagag ggcttaaagg aattagaata cagaggctat gacagcgcgg gtttagccgt attgagcaat gatcgtttgg aagtgtttaa aactcaaggg aaattagaaa accttaaatc agagcttaaa aataaagagt ttttggattt tggcgtgagt atcgctcaca ccagatgggc aacgcatggc aagccaagca gcgcgaacgc ccacccgcat tttacagaaa atttagcctt agtgcataat ggcatcattg aaaattacgc gagtttgaaa aaggaattgg aaaacaaagg gcatgcgttt ttgagccaaa cggacacgga agtgattgca catttattag aagaaacgct taaaagcgag agcgatttat tgaaagcttt tgaaaaaagc atcagccttt taaaagggag ttatgcgatt ttaatgctcc ataaaagggc taaagagagc ttattttacg ctaaatcttc ttcgccttta gttgtgggta agggcaaaga aggggtgttt tttgcgtcca gtttgagcgt gttagcccct aaagtggatc aatttgtcat tttagaagaa aacagcgtgg ggcggatttc tttagaaaat tttaaagatt taaacaatat tgaaaacatg aaagattacg cttttgagga taaggattat tctaaaggca attttaggaa ttatttagaa aaagagattt atgagcagca tagcagtttg ttagagtgtt tagaggggcg cttggaagcc ttgaatgtgt attgcgagat cgatcctgaa tttttagaga atgtgaatga aatcacgctg tgttcttgcg ggagcagtta ccatgcgagt ttggcgagcg tgtatttgtt tgaaaggtta gccaaaataa gagcgagagc cattttagcg agcgaatacc gctacgccca ttttaaaagc aaccctaacg agctttttat agcgatttct caaagcggcg aaaccgctga tactttggag gctttaaaat tagccaaagc ccaagggctt aaaaccatta gcttgtgtaa cgcccctttt agcatgatga gccgcatcag cgatcacacg cttttgatta gagcgggggt agaaaggagc gtggcatcca ctaaggcgtt ttcttcgcaa gtgatgcttt tatggctttt gagcgtgtat ataggcaaac agctagggac catctctaaa gaagaagaaa gaatccaggc taaaaacatg ctcaatagcg tgaaggcgat gaaagtagag cctaaattgc atgaaaagat caagcgttta tccaaacgct acttgcatgg gcatggcttt ttttatatcg gccgcgatgt gttttacccg ctcgctttag aaggggcgct aaaacttaaa gaaatcagct acttgcacgc tgaggggtat gcgagcgcgg agatgaagca tggacctatt gcgttagtgg attctaacct ttttaccatt gctttattgt ctaagcactt gttatttgat aaaaccaaaa gcaatattga agaattgagc gctagggatt ctacgatttg cgtgttaagc tctgaaattt tagaaattgc tgatgatttt atccaattag aagagagcga aagctacatg gaagaatttt tccgcatgaa tttagcggtg cagcttttag ccttagaaat cgctatgcgt ttaaatcatg atgtggatca cccaagaaac ttagctaaaa gcgttaccgt ggaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat