HepyC.17922.a

50S ribosomal protein L2

CENTER ID: HepyC.17922.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17922.a.B1.GE41108 full length 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1371
UniProt: B5Z8W4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIKTYKPYT PSRRFMSVLD SKDITAKSSV KGLLTKLKAT AGRNNNGRIT SRHKERGAKK LYRIIDFKRN KYNIEGKVAA IEYDPYRNAR IALVVYPDGD KRYILQPSGL KVGDSVIAAE GGLDIKVGFA MKLKNIPIGT VVHNIEMHPG AGGQLARSAG MSAQIMGREN KYTILRMPSS EMRYILSECM ASVGVVGNED FINVSIGKAG RNRHRGIRPQ TRGSAMNPVD HPHGGGEGKT GTSGHPVSPW GTPAKGYKTR KKKASDKLII SRKKHK
NT Sequence
atggcgatta aaacttataa gccctacacc ccaagcagac gcttcatgtc ggtgttggac tctaaagaca ttaccgcaaa aagcagtgtc aaaggcttac tcactaagct taaagcgaca gcagggagga acaataatgg gcgaatcacc agccgccaca aagaaagagg ggctaaaaaa ctctatcgca ttattgattt caaacgcaac aaatacaaca ttgaagggaa agtggctgcg attgagtatg atccttacag aaacgcgcgt atcgctcttg tggtctatcc tgatggggac aaacgctata ttttacagcc aagcggtttg aaagtgggcg atagcgttat cgctgctgaa ggcggtttgg atattaaagt gggctttgcg atgaagttaa aaaatatccc tataggaacg gtggtgcata acattgaaat gcatccgggg gctggcgggc aattagccag aagcgcgggg atgagcgctc aaatcatggg tagggaaaat aaatacacta tccttagaat gccaagctct gaaatgcgct acattttaag cgaatgcatg gcgagtgttg gcgtggtagg gaatgaggat tttatcaatg tctctatcgg taaggcaggg cgtaaccgcc atagaggcat tcgcccacaa actcgtggga gcgcgatgaa cccagtggat cacccgcatg gtgggggtga gggtaaaaca gggacaagcg gtcatcctgt atcgccttgg ggcactccag ctaagggcta taaaacgaga aagaaaaaag ctagcgacaa gctcatcatt tccagaaaga aacataaata a
Details for HepyC.17922.a.B1.GE41108
HARVESTED ON: data unavailable
SEQUENCED ON: 2/13/2017
EXPECTED MW: 31kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA IKTYKPYTPS RRFMSVLDSK DITAKSSVKG LLTKLKATAG RNNNGRITSR HKERGAKKLY RIIDFKRNKY NIEGKVAAIE YDPYRNARIA LVVYPDGDKR YILQPSGLKV GDSVIAAEGG LDIKVGFAMK LKNIPIGTVV HNIEMHPGAG GQLARSAGMS AQIMGRENKY TILRMPSSEM RYILSECMAS VGVVGNEDFI NVSIGKAGRN RHRGIRPQTR GSAMNPVDHP HGGGEGKTGT SGHPVSPWGT PAKGYKTRKK KASDKLIXXE RNINK
Validated NT Sequence
atcttactca cttatttatg tttctttcnn nnatgatgag cttgtcgcta gcttttttct ttctcgtttt atagccctta gctggagtgc cccaaggcga tacaggatga ccgcttgtcc ctgttttacc ctcaccccca ccatgcgggt gatccactgg gttcatcgcg ctcccacgag tttgtgggcg aatgcctcta tggcggttac gccctgcctt accgatagag acattgataa aatcctcatt ccctaccacg ccaacactcg ccatgcattc gcttaaaatg tagcgcattt cagagcttgg cattctaagg atagtgtatt tattttccct acccatgatt tgagcgctca tccccgcgct tctggctaat tgcccgccag cccccggatg catttcaatg ttatgcacca ccgttcctat agggatattt tttaacttca tcgcaaagcc cactttaata tccaaaccgc cttcagcagc gataacgcta tcgcccactt tcaaaccgct tggctgtaaa atatagcgtt tgtccccatc aggatagacc acaagagcga tacgcgcgtt tctgtaagga tcatactcaa tcgcagccac tttcccttca atgttgtatt tgttgcgttt gaaatcaata atgcgataga gttttttagc ccctctttct ttgtggcggc tggtgattcg cccattattg ttcctccctg ctgtcgcttt aagcttagtg agtaagcctt tgacactgct ttttgcggta atgtctttag agtccaacac cgacatgaag cgtctgcttg gggtgtaggg cttataagtt ttaatcgcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMA IKTYKPYTPS RRFMSVLDSK DITAKSSVKG LLTKLKATAG RNNNGRITSR HKERGAKKLY RIIDFKRNKY NIEGKVAAIE YDPYRNARIA LVVYPDGDKR YILQPSGLKV GDSVIAAEGG LDIKVGFAMK LKNIPIGTVV HNIEMHPGAG GQLARSAGMS AQIMGRENKY TILRMPSSEM RYILSECMAS VGVVGNEDFI NVSIGKAGRN RHRGIRPQTR GSAMNPVDHP HGGGEGKTGT SGHPVSPWGT PAKGYKTRKK KASDKLIISR KKHK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga ttaaaactta taagccctac accccaagca gacgcttcat gtcggtgttg gactctaaag acattaccgc aaaaagcagt gtcaaaggct tactcactaa gcttaaagcg acagcaggga ggaacaataa tgggcgaatc accagccgcc acaaagaaag aggggctaaa aaactctatc gcattattga tttcaaacgc aacaaataca acattgaagg gaaagtggct gcgattgagt atgatcctta cagaaacgcg cgtatcgctc ttgtggtcta tcctgatggg gacaaacgct atattttaca gccaagcggt ttgaaagtgg gcgatagcgt tatcgctgct gaaggcggtt tggatattaa agtgggcttt gcgatgaagt taaaaaatat ccctatagga acggtggtgc ataacattga aatgcatccg ggggctggcg ggcaattagc cagaagcgcg gggatgagcg ctcaaatcat gggtagggaa aataaataca ctatccttag aatgccaagc tctgaaatgc gctacatttt aagcgaatgc atggcgagtg ttggcgtggt agggaatgag gattttatca atgtctctat cggtaaggca gggcgtaacc gccatagagg cattcgccca caaactcgtg ggagcgcgat gaacccagtg gatcacccgc atggtggggg tgagggtaaa acagggacaa gcggtcatcc tgtatcgcct tggggcactc cagctaaggg ctataaaacg agaaagaaaa aagctagcga caagctcatc atttccagaa agaaacataa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat