HepyC.17901.b

Peptide chain release factor 2 (RF-2)

CENTER ID: HepyC.17901.b
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17901.b.B1.GE41106 full length 1 363
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.177
UniProt: B5Z9U4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDNYTYSELL KSLQNKCDNI ALIIKPEKIK QELERIEKEQ EDPNFWQDVL KARDTNKEKV RLNRLLETYQ KTKNSLDESV ELFELAQNDN DETTLSLLYE EAPTLEHSVQ KVEIEIMLSG ENDASNAIIT IQPGAGGTES QDWASILYRM YLRWAERRGF KSEILDYQDG EEAGIKGVAF IIKGENAYGY LKNENGVHRL VRISPFDANA KRHTSFASVQ ISPELDDDID IEIDEKDVRY DYYRASGAGG QHVNKTESAV RITHFPTGIV VQCQNDRSQH KNKASALKML KSKLYELELE KQQSSAKNEE KSEIGWGHQI RSYVLAPYQQ VKDARSNIAY SNVEAILDGD IDAILEGVLI AKA
NT Sequence
gtggataact acacctatag cgaattgtta aaaagcttgc aaaacaaatg cgataatatc gctttaatca tcaagcctga aaagatcaag caagaattag aacgcattga aaaagagcaa gaagacccta atttttggca agatgtctta aaggctagag acaccaataa agaaaaagtg cgcctaaacc gcttgctaga aacctatcaa aaaaccaaaa actctttaga tgaaagcgtg gaattgtttg aactcgctca aaacgataac gatgagacta ctttatcctt gctctatgaa gaggctccta ctttagaaca cagcgtccaa aaagtagaaa ttgaaatcat gttaagcggt gaaaatgacg cttcaaacgc tattattacc attcagcctg gagcgggggg gactgaaagc caggattggg cgagtatttt gtatcgcatg tatttgaggt gggcggaaag aagaggtttt aaaagcgaga ttttagatta tcaagatggc gaagaagcgg gcattaaagg ggtcgccttt atcattaagg gcgaaaacgc ttatggctat ttgaaaaatg aaaacggggt gcataggctt gtaaggatct cgccctttga tgcgaacgcc aaacggcaca cgagttttgc gagcgtgcaa attagccctg aattagacga tgatattgat atagaaattg atgaaaaaga tgtccgttat gattattaca gagccagtgg ggcaggcggt cagcatgtca ataaaacaga aagcgcggtt agaatcacgc atttccctac cggcattgtg gtgcaatgcc aaaacgacag gagtcagcat aaaaacaaag cgagcgcgtt aaaaatgctt aaatccaagc tctatgaatt ggaattagaa aagcagcaaa gcagcgccaa aaatgaagaa aaaagcgaga ttggttgggg gcatcaaata agaagctatg tcctagcccc ctaccagcaa gtcaaagacg cgcgctccaa tattgcttat agcaatgtgg aagcgatact agatggcgat attgatgcga ttttagaggg cgttttgatt gctaaagctt aa
Details for HepyC.17901.b.B1.GE41106
HARVESTED ON: data unavailable
SEQUENCED ON: 2/13/2017
EXPECTED MW: 42kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV DNYTYSELLK SLQNKCDNIA LIIKPEKIKQ ELERIEKEQE DPNFWQDVLK ARDTNKEKVR LNRLLETYQK TKNSLDESVE LFELAQNDND ETTLSLLYEE APTLEHSVQK VEIEIMLSGE NDASNAIITI QPGAGGTESQ DWASILYRMY LRWAERRGFK SEILDYQDGE EAGIKGVAFI IKGENAYGYL KNENGVHRLV RISPFDANAK RHTSFASVQI SPELDDDIDI EIDEKDVRYD YYRASGAGGQ HVNKTESAVR ITHFPTGIVV QCQNDRSQHK NKASALKMLK SKLYELELEK QQSSAKNEEK SEIGWGHQIR SYVLAPYQQV KDARSNIAYS NVEAILDGDI DAIL
Validated NT Sequence
ctaaaatcgc atcaatatcg ccatctagta tcgcttccac attgctataa gcaatattgg agcgcgcgtc tttgacttgc tggtaggggg ctaggacata gcttcttatt tgatgccccc aaccaatctc gcttttttct tcatttttgg cgctgctttg ctgcttttct aattccaatt catagagctt ggatttaagc atttttaacg cgctcgcttt gtttttatgc tgactcctgt cgttttggca ttgcaccaca atgccggtag ggaaatgcgt gattctaacc gcgctttctg ttttattgac atgctgaccg cctgccccac tggctctgta ataatcataa cggacatctt tttcatcaat ttctatatca atatcatcgt ctaattcagg gctaatttgc acgctcgcaa aactcgtgtg ccgtttggcg ttcgcatcaa agggcgagat ccttacaagc ctatgcaccc cgttttcatt tttcaaatag ccataagcgt tttcgccctt aatgataaag gcgacccctt taatgcccgc ttcttcgcca tcttgataat ctaaaatctc gcttttaaaa cctcttcttt ccgcccacct caaatacatg cgatacaaaa tactcgccca atcctggctt tcagtccccc ccgctccagg ctgaatggta ataatagcgt ttgaagcgtc attttcaccg cttaacatga tttcaatttc tactttttgg acgctgtgtt ctaaagtagg agcctcttca tagagcaagg ataaagtagt ctcatcgtta tcgttttgag cgagttcaaa caattccacg ctttcatcta aagagttttt ggttttttga taggtttcta gcaagcggtt taggcgcact ttttctttat tggtgtctct agcctttaag acatcttgcc aaaaattagg gtcttcttgc tctttttcaa tgcgttctaa ttcttgcttg atcttttcag gcttgatgat taaagcgata ttatcgcatt tgttttgcaa gctttttaac aattcgctat aggtgtagtt atccaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMD NYTYSELLKS LQNKCDNIAL IIKPEKIKQE LERIEKEQED PNFWQDVLKA RDTNKEKVRL NRLLETYQKT KNSLDESVEL FELAQNDNDE TTLSLLYEEA PTLEHSVQKV EIEIMLSGEN DASNAIITIQ PGAGGTESQD WASILYRMYL RWAERRGFKS EILDYQDGEE AGIKGVAFII KGENAYGYLK NENGVHRLVR ISPFDANAKR HTSFASVQIS PELDDDIDIE IDEKDVRYDY YRASGAGGQH VNKTESAVRI THFPTGIVVQ CQNDRSQHKN KASALKMLKS KLYELELEKQ QSSAKNEEKS EIGWGHQIRS YVLAPYQQVK DARSNIAYSN VEAILDGDID AILEGVLIAK A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggata actacaccta tagcgaattg ttaaaaagct tgcaaaacaa atgcgataat atcgctttaa tcatcaagcc tgaaaagatc aagcaagaat tagaacgcat tgaaaaagag caagaagacc ctaatttttg gcaagatgtc ttaaaggcta gagacaccaa taaagaaaaa gtgcgcctaa accgcttgct agaaacctat caaaaaacca aaaactcttt agatgaaagc gtggaattgt ttgaactcgc tcaaaacgat aacgatgaga ctactttatc cttgctctat gaagaggctc ctactttaga acacagcgtc caaaaagtag aaattgaaat catgttaagc ggtgaaaatg acgcttcaaa cgctattatt accattcagc ctggagcggg ggggactgaa agccaggatt gggcgagtat tttgtatcgc atgtatttga ggtgggcgga aagaagaggt tttaaaagcg agattttaga ttatcaagat ggcgaagaag cgggcattaa aggggtcgcc tttatcatta agggcgaaaa cgcttatggc tatttgaaaa atgaaaacgg ggtgcatagg cttgtaagga tctcgccctt tgatgcgaac gccaaacggc acacgagttt tgcgagcgtg caaattagcc ctgaattaga cgatgatatt gatatagaaa ttgatgaaaa agatgtccgt tatgattatt acagagccag tggggcaggc ggtcagcatg tcaataaaac agaaagcgcg gttagaatca cgcatttccc taccggcatt gtggtgcaat gccaaaacga caggagtcag cataaaaaca aagcgagcgc gttaaaaatg cttaaatcca agctctatga attggaatta gaaaagcagc aaagcagcgc caaaaatgaa gaaaaaagcg agattggttg ggggcatcaa ataagaagct atgtcctagc cccctaccag caagtcaaag acgcgcgctc caatattgct tatagcaatg tggaagcgat actagatggc gatattgatg cgattttaga gggcgttttg attgctaaag cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat