SSGCID: Target Page

HepyC.17901.a

Peptide chain release factor 1 (RF-1)

CENTER ID:	HepyC.17901.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.17901.a.B1.GE41105	full length		1	352

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.17901.a.B1.PW38405	full length		1	352

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.83
UniProt:	B5Z680

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSILAEKLSS ILKRYDELTA LLSSAEVISD IKKLTELSKE QSSIEEISIA SKEYLSVLED IKENKELLED KELSELAKEE LKILETKKSD LETAIKQLLI PKDPNDDKNI YLELRAGTGG DEAGIFVGDL FKAYCRYADL KKWKVEIVSS SENSVGGYKE IIALIKGKGV YSRLKFEAGT HRVQRVPETE SQGRIHTSAI TVAIMPEVDD VEVSINPSDL KIEVFRAGGH GGQCVNTTDS AVRITHLPTN ISVSMQDEKS QHKNKDKALK ILKARLYEKQ IEEQQLANAK DRKEQVGSGD RSERIRTYNY PQNRLSEHRI NLTLYSLEEI MLSGNLDEVI NPLIAHAQSQ FE

NT Sequence

atgtctattc tagctgaaaa gctttcttcc attctcaaac gatacgacga actcacagcg ttgctttcta gcgctgaagt gattagcgat attaaaaaac tcaccgaatt gagcaaagag caaagctcca ttgaagaaat ctctatagcg agtaaagaat atttgagcgt tttagaggat atcaaagaaa ataaagagct tttagaagac aaggaattga gcgaactggc taaagaagag ttaaaaattt tagaaactaa aaaaagcgat ctagaaaccg ccattaagca actccttatc cccaaagacc ctaacgacga taaaaacatc tatttagagt taagggccgg cacggggggc gatgaagcgg gcatttttgt aggggatttg tttaaagcgt attgccgtta tgcggatttg aaaaaatgga aagtagagat agtaagctct agcgaaaaca gcgtaggggg ctataaagaa atcatcgctt tgattaaggg caagggcgtg tattcaaggc tcaaatttga agcaggcacg catcgagtcc aaagagtccc tgaaacagaa tctcaagggc gcatccacac ttccgctatt acagtagcga tcatgcctga agtggatgat gtggaagtct ctatcaaccc tagcgattta aagattgaag tgtttcgtgc tggcgggcat ggggggcaat gcgtcaatac cacagactct gcggtgcgca tcacgcacct ccccaccaat atcagcgtga gcatgcaaga tgaaaaatcc cagcataaaa acaaggataa agctctaaaa atcctaaaag cgcgccttta tgaaaaacaa attgaagagc aacagctcgc taacgccaaa gaccgaaagg agcaagtggg tagtggggat aggagcgaaa ggatccgcac ttataattac ccgcaaaacc gcttgagcga acatagaatc aatttaactc tgtatagttt agaagaaatc atgctttcag ggaatttaga tgaagtgatc aaccctttaa tcgctcatgc tcaaagccag tttgaataa

Details for HepyC.17901.a.B1.GE41105

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS ILAEKLSSIL KRYDELTALL SSAEVISDIK KLTELSKEQS SIEEISIASK EYLSVLEDIK ENKELLEDKE LSELAKEELK ILETKKSDLE TAIKQLLIPK DPNDDKNIYL ELRAGTGGDE AGIFVGDLFK AYCRYADLKK WKVEIVSSSE NSVGGYKEII ALIKGKGVYS RLKFEAGTHR VQRVPETESQ GRIHTSAITV AIMPEVDDVE VSINPSDLKI EVFRAGGHGG QCVNTTDSAV RITHLPTNIS VSMQDEKSQH KNKDKALKIL KARLYEKQIE EQQLANAKDR KEQVGSGDRS ERIRTYNYPQ NRLSEHRINL TLYSLEEIML SGNLDEVINP LI

Validated NT Sequence

gcgattaaag ggttgatcac ttcatctaaa ttccctgaaa gcatgatttc ttctaaacta tacagagtta aattgattct atgttcgctc aagcggtttt gcgggtaatt ataagtgcgg atcctttcgc tcctatcccc actacccact tgctcctttc ggtctttggc gttagcgagc tgttgctctt caatttgttt ttcataaagg cgcgctttta ggatttttag agctttatcc ttgtttttat gctgggattt ttcatcttgc atgctcacgc tgatattggt ggggaggtgc gtgatgcgca ccgcagagtc tgtggtattg acgcattgcc ccccatgccc gccagcacga aacacttcaa tctttaaatc gctagggttg atagagactt ccacatcatc cacttcaggc atgatcgcta ctgtaatagc ggaagtgtgg atgcgccctt gagattctgt ttcagggact ctttggactc gatgcgtgcc tgcttcaaat ttgagccttg aatacacgcc cttgccctta atcaaagcga tgatttcttt atagccccct acgctgtttt cgctagagct tactatctct actttccatt ttttcaaatc cgcataacgg caatacgctt taaacaaatc ccctacaaaa atgcccgctt catcgccccc cgtgccggcc cttaactcta aatagatgtt tttatcgtcg ttagggtctt tggggataag gagttgctta atggcggttt ctagatcgct ttttttagtt tctaaaattt ttaactcttc tttagccagt tcgctcaatt ccttgtcttc taaaagctct ttattttctt tgatatcctc taaaacgctc aaatattctt tactcgctat agagatttct tcaatggagc tttgctcttt gctcaattcg gtgagttttt taatatcgct aatcacttca gcgctagaaa gcaacgctgt gagttcgtcg tatcgtttga gaatggaaga aagcttttca gctagaatag acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta ttctagctga aaagctttct tccattctca aacgatacga cgaactcaca gcgttgcttt ctagcgctga agtgattagc gatattaaaa aactcaccga attgagcaaa gagcaaagct ccattgaaga aatctctata gcgagtaaag aatatttgag cgttttagag gatatcaaag aaaataaaga gcttttagaa gacaaggaat tgagcgaact ggctaaagaa gagttaaaaa ttttagaaac taaaaaaagc gatctagaaa ccgccattaa gcaactcctt atccccaaag accctaacga cgataaaaac atctatttag agttaagggc cggcacgggg ggcgatgaag cgggcatttt tgtaggggat ttgtttaaag cgtattgccg ttatgcggat ttgaaaaaat ggaaagtaga gatagtaagc tctagcgaaa acagcgtagg gggctataaa gaaatcatcg ctttgattaa gggcaagggc gtgtattcaa ggctcaaatt tgaagcaggc acgcatcgag tccaaagagt ccctgaaaca gaatctcaag ggcgcatcca cacttccgct attacagtag cgatcatgcc tgaagtggat gatgtggaag tctctatcaa ccctagcgat ttaaagattg aagtgtttcg tgctggcggg catggggggc aatgcgtcaa taccacagac tctgcggtgc gcatcacgca cctccccacc aatatcagcg tgagcatgca agatgaaaaa tcccagcata aaaacaagga taaagctcta aaaatcctaa aagcgcgcct ttatgaaaaa caaattgaag agcaacagct cgctaacgcc aaagaccgaa aggagcaagt gggtagtggg gataggagcg aaaggatccg cacttataat tacccgcaaa accgcttgag cgaacataga atcaatttaa ctctgtatag tttagaagaa atcatgcttt cagggaattt agatgaagtg atcaaccctt taatcgctca tgctcaaagc cagtttgaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for HepyC.17901.a.B1.PW38405

PURIFICATION DATe:	12/11/2017
CONCENTRATION:	45.67mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTA TTCTAGCTGA AAAGCTTTCT TCCATTCTCA AACGATACGA CGAACTCACA GCGTTGCTTT CTAGCGCTGA AGTGATTAGC GATATTAAAA AACTCACCGA ATTGAGCAAA GAGCAAAGCT CCATTGAAGA AATCTCTATA GCGAGTAAAG AATATTTGAG CGTTTTAGAG GATATCAAAG AAAATAAAGA GCTTTTAGAA GACAAGGAAT TGAGCGAACT GGCTAAAGAA GAGTTAAAAA TTTTAGAAAC TAAAAAAAGC GATCTAGAAA CCGCCATTAA GCAACTCCTT ATCCCCAAAG ACCCTAACGA CGATAAAAAC ATCTATTTAG AGTTAAGGGC CGGCACGGGG GGCGATGAAG CGGGCATTTT TGTAGGGGAT TTGTTTAAAG CGTATTGCCG TTATGCGGAT TTGAAAAAAT GGAAAGTAGA GATAGTAAGC TCTAGCGAAA ACAGCGTAGG GGGCTATAAA GAAATCATCG CTTTGATTAA GGGCAAGGGC GTGTATTCAA GGCTCAAATT TGAAGCAGGC ACGCATCGAG TCCAAAGAGT CCCTGAAACA GAATCTCAAG GGCGCATCCA CACTTCCGCT ATTACAGTAG CGATCATGCC TGAAGTGGAT GATGTGGAAG TCTCTATCAA CCCTAGCGAT TTAAAGATTG AAGTGTTTCG TGCTGGCGGG CATGGGGGGC AATGCGTCAA TACCACAGAC TCTGCGGTGC GCATCACGCA CCTCCCCACC AATATCAGCG TGAGCATGCA AGATGAAAAA TCCCAGCATA AAAACAAGGA TAAAGCTCTA AAAATCCTAA AAGCGCGCCT TTATGAAAAA CAAATTGAAG AGCAACAGCT CGCTAACGCC AAAGACCGAA AGGAGCAAGT GGGTAGTGGG GATAGGAGCG AAAGGATCCG CACTTATAAT TACCCGCAAA ACCGCTTGAG CGAACATAGA ATCAATTTAA CTCTGTATAG TTTAGAAGAA ATCATGCTTT CAGGGAATTT AGATGAAGTG ATCAACCCTT TAATCGCTCA TGCTCAAAGC CAGTTTGAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT