SSGCID: Target Page

HepyC.17894.a

30S ribosomal protein S2

CENTER ID:	HepyC.17894.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.17894.a.B1.GE41104	full length		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1618
UniProt:	B5Z9I5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKDLLECGVH FGHQTRRWNP KTKKFIFGVR KNIHIIDLQK TLRYFRYTYN IVRDASAQGK SIMFVGTKKQ ANETLKEFAE SIQVPYVNYR WLGGMLTNFS TIRKSVRKLE IIEEMENSGQ IDLLTKKEKL MILRKKEKLD KYLGGVRHMK KIPDMIFVID VAKEKIAVAE ARKLHIPIVA PLDTNCDPDL VDYPIPGNDD AIRSIRLFCK EMSEAILEGR ELMQEEIVHA DENSEEIEFV SNEEKEEMLA EIQKEITQGA E

NT Sequence

atgaaagatt tattagaatg cggtgtgcat tttggacacc aaacaaggcg ttggaaccct aaaaccaaaa aattcatttt tggcgttagg aaaaatatcc atattattga tttgcaaaaa accttgcgct attttagata cacttataat atcgtgcgcg atgcgagcgc tcaaggcaaa agcatcatgt ttgtaggcac taaaaaacaa gccaatgaga ctttgaaaga atttgctgaa agcattcaag tcccttatgt caattaccgc tggcttggtg gcatgctgac taattttagc accattagaa aatcggttag gaaattagaa atcattgaag aaatggaaaa tagcggtcaa attgatttat tgactaaaaa agaaaagctc atgattttaa ggaaaaaaga aaagctggat aaatatcttg gcggggtgcg ccacatgaaa aaaatccctg atatgatttt tgtgattgat gtggctaaag aaaaaatcgc tgtcgctgaa gcaagaaaac tccatattcc tatcgtggct cccttggaca ctaactgcga tcctgatctg gtggattacc ccattcctgg aaatgacgat gcgatccgct ctatcaggct attttgtaaa gaaatgagcg aagcgatttt agaggggcga gaactcatgc aagaagaaat cgtccatgcg gatgaaaata gcgaagagat agagtttgtg agcaatgaag aaaaagaaga aatgctcgct gaaatccaaa aagaaatcac tcaaggagct gaataa

Details for HepyC.17894.a.B1.GE41104

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK DLLECGVHFG HQTRRWNPKT KKFIFGVRKN IHIIDLQKTL RYFRYTYNIV RDASAQGKSI MFVGTKKQAN ETLKEFAESI QVPYVNYRWL GGMLTNFSTI RKSVRKLEII EEMENSGQID LLTKKEKLMI LRKKEKLDKY LGGVRHMKKI PDMIFVIDVA KEKIAVAEAR KLHIPIVAPL DTNCDPDLVD YPIPGNDDAI RSIRLFCKEM SEAILEGREL MQEEIVHADE NSEEIEFVSN EEKEEMLAEI QKEITQGAE

Validated NT Sequence

atcttactca cttattcagc tccttgagtg atttcttttt ggatttcagc gagcatttct tctttttctt cattgctcac aaactctatc tcttcgctat tttcatccgc atggacgatt tcttcttgca tgagttctcg cccctctaaa atcgcttcgc tcatttcttt acaaaatagc ctgatagagc ggatcgcatc gtcatttcca ggaatggggt aatccaccag atcaggatcg cagttagtgt ccaagggagc cacgatagga atatggagtt ttcttgcttc agcgacagcg attttttctt tagccacatc aatcacaaaa atcatatcag ggattttttt catgtggcgc accccgccaa gatatttatc cagcttttct tttttcctta aaatcatgag cttttctttt ttagtcaata aatcaatttg accgctattt tccatttctt caatgatttc taatttccta accgattttc taatggtgct aaaattagtc agcatgccac caagccagcg gtaattgaca taagggactt gaatgctttc agcaaattct ttcaaagtct cattggcttg ttttttagtg cctacaaaca tgatgctttt gccttgagcg ctcgcatcgc gcacgatatt ataagtgtat ctaaaatagc gcaaggtttt ttgcaaatca ataatatgga tatttttcct aacgccaaaa atgaattttt tggttttagg gttccaacgc cttgtttggt gtccaaaatg cacaccgcat tctaataaat ctttcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag atttattaga atgcggtgtg cattttggac accaaacaag gcgttggaac cctaaaacca aaaaattcat ttttggcgtt aggaaaaata tccatattat tgatttgcaa aaaaccttgc gctattttag atacacttat aatatcgtgc gcgatgcgag cgctcaaggc aaaagcatca tgtttgtagg cactaaaaaa caagccaatg agactttgaa agaatttgct gaaagcattc aagtccctta tgtcaattac cgctggcttg gtggcatgct gactaatttt agcaccatta gaaaatcggt taggaaatta gaaatcattg aagaaatgga aaatagcggt caaattgatt tattgactaa aaaagaaaag ctcatgattt taaggaaaaa agaaaagctg gataaatatc ttggcggggt gcgccacatg aaaaaaatcc ctgatatgat ttttgtgatt gatgtggcta aagaaaaaat cgctgtcgct gaagcaagaa aactccatat tcctatcgtg gctcccttgg acactaactg cgatcctgat ctggtggatt accccattcc tggaaatgac gatgcgatcc gctctatcag gctattttgt aaagaaatga gcgaagcgat tttagagggg cgagaactca tgcaagaaga aatcgtccat gcggatgaaa atagcgaaga gatagagttt gtgagcaatg aagaaaaaga agaaatgctc gctgaaatcc aaaaagaaat cactcaagga gctgaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t