HepyC.17885.a

Aspartate-semialdehyde dehydrogenase

CENTER ID: HepyC.17885.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17885.a.B1.GE41100 full length 1 268
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1234
UniProt: B5Z8I5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEEFATSAA KTALVVDNTS FFRLHKDVPL VVPEINAKEI FNAPLNIIAN PNCSTIQMTQ ILNPLHLHFK IKSVIVSTYQ AVSGAGNKGI ESLKNELKTA LECLEKDPTI DLNQILQAGA FPYPIAFNAI AHIDVFKENG YTKEELKMVH ETHKIMGVDF PISATCVRVP VLRSHSESLS IAFEKEFDLK EVYEVLKNAP SVVVCDDPSH NLYPTPLKAS HTDSVFIGRL RKDLFDKKTL HGFCVADQLR VGAATNALKI ALHYIKNA
NT Sequence
gtgagcgaag aatttgctac aagcgctgca aaaacggcct tagtggttga taacacgagc ttttttagat tgcataaaga tgtgccttta gtcgtgcctg aaatcaacgc taaagaaatt tttaacgctc ctttgaatat catcgctaac cctaattgct ctaccattca aatgacgcaa attttaaacc ctttacacct tcattttaag ataaaaagcg ttattgttag cacctatcaa gccgtgagtg gggcagggaa caagggcata gagagcttaa aaaatgaatt aaaaaccgca ttagagtgtt tggaaaaaga ccctactatt gatttaaacc aaatcttgca agccggggct ttcccttacc cgatcgcttt caatgcgatc gctcatattg atgtttttaa ggagaatggc tacacgaaag aagagctaaa aatggtgcat gaaacccata aaatcatggg cgtggatttt cctatcagcg cgacttgcgt gcgcgtgccg gtattgagga gtcatagcga aagtttgagt atcgcttttg aaaaagaatt cgatctcaaa gaagtctatg aagttttaaa aaacgcccct agcgtggttg tttgcgatga tcctagccac aatctctacc ccacgccctt aaaagcgagc cacacggata gcgtctttat agggcgcttg aggaaggatt tgtttgataa gaagactttg cacggctttt gcgtggcgga tcaattaaga gtgggggcag ccaccaatgc gctcaaaatc gctctgcatt acattaagaa cgcttga
Details for HepyC.17885.a.B1.GE41100
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 31kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV SEEFATSAAK TALVVDNTSF FRLHKDVPLV VPEINAKEIF NAPLNIIANP NCSTIQMTQI LNPLHLHFKI KSVIVSTYQA VSGAGNKGIE SLKNELKTAL ECLEKDPTID LNQILQAGAF PYPIAFNAIA HIDVFKENGY TKEELKMVHE THKIMGVDFP ISATCVRVPV LRSHSESLSI AFEKEFDLKE VYEVLKNAPS VVVCDDPSHN LYPTPLKASH TDSVFIGRLR KDLFDKKTLH GFCVADQLRV GAATNALKIA LXXIKNA
Validated NT Sequence
atcttactca cttaagcgtt cttaatgnnn nnnagagcga ttttgagcgc attggtggct gcccccactc ttaattgatc cgccacgcaa aagccgtgca aagtcttctt atcaaacaaa tccttcctca agcgccctat aaagacgcta tccgtgtggc tcgcttttaa gggcgtgggg tagagattgt ggctaggatc atcgcaaaca accacgctag gggcgttttt taaaacttca tagacttctt tgagatcgaa ttctttttca aaagcgatac tcaaactttc gctatgactc ctcaataccg gcacgcgcac gcaagtcgcg ctgataggaa aatccacgcc catgatttta tgggtttcat gcaccatttt tagctcttct ttcgtgtagc cattctcctt aaaaacatca atatgagcga tcgcattgaa agcgatcggg taagggaaag ccccggcttg caagatttgg tttaaatcaa tagtagggtc tttttccaaa cactctaatg cggtttttaa ttcatttttt aagctctcta tgcccttgtt ccctgcccca ctcacggctt gataggtgct aacaataacg ctttttatct taaaatgaag gtgtaaaggg tttaaaattt gcgtcatttg aatggtagag caattagggt tagcgatgat attcaaagga gcgttaaaaa tttctttagc gttgatttca ggcacgacta aaggcacatc tttatgcaat ctaaaaaagc tcgtgttatc aaccactaag gccgtttttg cagcgcttgt agcaaattct tcgctcacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS EEFATSAAKT ALVVDNTSFF RLHKDVPLVV PEINAKEIFN APLNIIANPN CSTIQMTQIL NPLHLHFKIK SVIVSTYQAV SGAGNKGIES LKNELKTALE CLEKDPTIDL NQILQAGAFP YPIAFNAIAH IDVFKENGYT KEELKMVHET HKIMGVDFPI SATCVRVPVL RSHSESLSIA FEKEFDLKEV YEVLKNAPSV VVCDDPSHNL YPTPLKASHT DSVFIGRLRK DLFDKKTLHG FCVADQLRVG AATNALKIAL HYIKNA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagcg aagaatttgc tacaagcgct gcaaaaacgg ccttagtggt tgataacacg agctttttta gattgcataa agatgtgcct ttagtcgtgc ctgaaatcaa cgctaaagaa atttttaacg ctcctttgaa tatcatcgct aaccctaatt gctctaccat tcaaatgacg caaattttaa accctttaca ccttcatttt aagataaaaa gcgttattgt tagcacctat caagccgtga gtggggcagg gaacaagggc atagagagct taaaaaatga attaaaaacc gcattagagt gtttggaaaa agaccctact attgatttaa accaaatctt gcaagccggg gctttccctt acccgatcgc tttcaatgcg atcgctcata ttgatgtttt taaggagaat ggctacacga aagaagagct aaaaatggtg catgaaaccc ataaaatcat gggcgtggat tttcctatca gcgcgacttg cgtgcgcgtg ccggtattga ggagtcatag cgaaagtttg agtatcgctt ttgaaaaaga attcgatctc aaagaagtct atgaagtttt aaaaaacgcc cctagcgtgg ttgtttgcga tgatcctagc cacaatctct accccacgcc cttaaaagcg agccacacgg atagcgtctt tatagggcgc ttgaggaagg atttgtttga taagaagact ttgcacggct tttgcgtggc ggatcaatta agagtggggg cagccaccaa tgcgctcaaa atcgctctgc attacattaa gaacgcttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at