HepyC.17552.a

Guanosine pentaphosphate phosphohydrolase

CENTER ID: HepyC.17552.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17552.a.B1.GE41023 full length 1 484
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.287
UniProt: B5ZA44

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKITTVIDI GSNSVRLAVF KKTSQFGFYL LFETKSKVRI SEGCYAFNGI LQEIPMQRAV KALSEFKEIA LKYKSKKILC VATSAVRDAP NRLEFVARVK KACGLQIKII DGQKEALYGG IACANLLHKN SGITIDIGGG STECALIEKG KIKDLISLDV GTIRIKEMFL DKDLDVKLAK AFIQKEVSKL PFKHKNAFGV GGTIRALSKV LMKRFDYPID SLHGYEIDAH KNLAFIEKIV MLKEDQLRLL GVNEERLDSI RSGALILSVV LEHLKTSLMI TSGVGVREGV FLSDLLRNHY HKFPPNINPS LISLKDRFLP HEKHSQKVKK ECVKLFEALS PLHKIDEKYL FHLKIAGELA SMGKILSVYL AHKHSAYFIL NALSYGFSHQ DRAIICLLAQ FSHKKIPKDN AIAHMSAMMP SLLTLQWLSF ILSLAENLCL TDSHHLKYTL EKNKLVIHSN DALYLAKEML PKLVKPIPLT IEFA
NT Sequence
atggctaaaa tcacaaccgt gattgatata ggctctaatt cagtgcgttt ggctgtcttt aaaaagacga gccagtttgg gttttacttg ctttttgaga ctaagtctaa ggttaggatt tcagagggtt gttatgcgtt taatggaatc ttgcaagaaa tccccatgca aagagccgtt aaagccttga gcgaatttaa agaaatcgct ctcaaataca aaagcaaaaa aatcttgtgc gtggcgacct cagcggtgcg cgatgcccct aacagattag agtttgtagc gagggtgaaa aaggcttgcg gtttgcaaat caaaatcatt gatgggcaaa aagaagcgct ctatggtggg attgcgtgcg cgaatttgtt gcataaaaat tcagggatca cgatagatat tggagggggt agcacggagt gcgcgttgat tgaaaaaggc aagattaagg acttaatctc gcttgatgtt gggacgattc gcattaaaga aatgttttta gacaaagact tagatgtcaa attggctaaa gcctttatcc aaaaagaagt ctctaaactg ccctttaagc ataaaaacgc ctttggggtg ggggggacga ttagggcgtt gagtaaagtg ctgatgaaac gctttgatta ccctattgat tctttgcatg gttatgaaat agatgcacat aaaaatttag cgttcattga aaaaatcgtc atgctcaaag aagatcaatt acggctttta ggggtgaatg aagagcgttt ggatagcatt aggagtgggg cgttgattct atcagtcgtt ttggagcatt taaaaacttc cttgatgatc actagcgggg tgggggtgag agaaggcgtg tttttgagcg atttattacg aaaccattac cataaattcc cccccaatat caacccctct ctcatctctt taaaagatcg ctttttgccc catgaaaagc acagccaaaa ggtcaaaaaa gaatgcgtga aattgtttga agccttatcg cctttgcata aaatagatga aaaatacctt ttccatttaa agattgcggg ggaattagcg agcatgggta agattttaag cgtctattta gcccacaagc acagcgcgta ttttattttg aacgctttga gttatggctt tagccaccag gatagagcga tcatttgctt attagcccaa ttcagccata aaaaaatccc taaagacaac gctatcgccc acatgagtgc gatgatgcca agccttttaa ccttacaatg gctgagtttt atcctttctt tagccgaaaa tttgtgccta acagacagcc atcatctaaa atacacgcta gaaaaaaaca agcttgtgat ccattctaat gatgcgcttt acttggctaa agagatgctc cctaaactcg ttaagcccat tcctttgacg atagagtttg cttga
Details for HepyC.17552.a.B1.GE41023
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHHM AKITTVIDIG SNSVRLAVFK KTSQFGFYLL FETKSKVRIS EGCYAFNGIL QEIPMQRAVK ALSEFKEIAL KYKSKKILCV ATSAVRDAPN RLEFVARVKK ACGLQIKIID GQKEALYGGI ACANLLHKNS GITIDIGGGS TECALIEKGK IKDLISLDVG TIRIKEMFLD KDLDVKLAKA FIQKEVSKLP FKHKNAFGVG GTIRALSKVL MKRFDYPIDS LHGYEIDAHK NLAFIEKIVM LKEDQLRLLG VNEERLDSIR SGALILSVVL EHLKTSLMIT SGVGVREGVF LSDLLRNHYH KFPPNINPSL ISLKDRFLPH EKHSQKVKKE CVKLFEALSP LHKIDEKYLF HLKIAGELAS MGKILSVYLA HKHSAYFILN ALSYGFSHQD RAIICLLAQF SHKKIPKDNA IAHMSAMMPS LLTLQWLSFI LSLAENLCLT DSHHLKYTLE KNKLVIHSND ALYLAKEMLP KLVK
Validated NT Sequence
ggcttaacga gtttagggag catctcttta gccaagtaaa gcgcatcatt agaatggatc acaagcttgt ttttttctag cgtgtatttt agatgatggc tgtctgttag gcacaaattt tcggctaaag aaaggataaa actcagccat tgtaaggtta aaaggcttgg catcatcgca ctcatgtggg cgatagcgtt gtctttaggg atttttttat ggctgaattg ggctaataag caaatgatcg ctctatcctg gtggctaaag ccataactca aagcgttcaa aataaaatac gcgctgtgct tgtgggctaa atagacgctt aaaatcttac ccatgctcgc taattccccc gcaatcttta aatggaaaag gtatttttca tctattttat gcaaaggcga taaggcttca aacaatttca cgcattcttt tttgaccttt tggctgtgct tttcatgggg caaaaagcga tcttttaaag agatgagaga ggggttgata ttggggggga atttatggta atggtttcgt aataaatcgc tcaaaaacac gccttctctc acccccaccc cgctagtgat catcaaggaa gtttttaaat gctccaaaac gactgataga atcaacgccc cactcctaat gctatccaaa cgctcttcat tcacccctaa aagccgtaat tgatcttctt tgagcatgac gattttttca atgaacgcta aatttttatg tgcatctatt tcataaccat gcaaagaatc aatagggtaa tcaaagcgtt tcatcagcac tttactcaac gccctaatcg tcccccccac cccaaaggcg tttttatgct taaagggcag tttagagact tctttttgga taaaggcttt agccaatttg acatctaagt ctttgtctaa aaacatttct ttaatgcgaa tcgtcccaac atcaagcgag attaagtcct taatcttgcc tttttcaatc aacgcgcact ccgtgctacc ccctccaata tctatcgtga tccctgaatt tttatgcaac aaattcgcgc acgcaatccc accatagagc gcttcttttt gcccatcaat gattttgatt tgcaaaccgc aagccttttt caccctcgct acaaactcta atctgttagg ggcatcgcgc accgctgagg tcgccacgca caagattttt ttgcttttgt atttgagagc gatttcttta aattcgctca aggctttaac ggctctttgc atggggattt cttgcaagat tccattaaac gcataacaac cctctgaaat cctaacctta gacttagtct caaaaagcaa gtaaaaccca aactggctcg tctttttaaa gacagccaaa cgcactgaat tagagcctat atcaatcacg gttgtgattt tagccatatg gtggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMA KITTVIDIGS NSVRLAVFKK TSQFGFYLLF ETKSKVRISE GCYAFNGILQ EIPMQRAVKA LSEFKEIALK YKSKKILCVA TSAVRDAPNR LEFVARVKKA CGLQIKIIDG QKEALYGGIA CANLLHKNSG ITIDIGGGST ECALIEKGKI KDLISLDVGT IRIKEMFLDK DLDVKLAKAF IQKEVSKLPF KHKNAFGVGG TIRALSKVLM KRFDYPIDSL HGYEIDAHKN LAFIEKIVML KEDQLRLLGV NEERLDSIRS GALILSVVLE HLKTSLMITS GVGVREGVFL SDLLRNHYHK FPPNINPSLI SLKDRFLPHE KHSQKVKKEC VKLFEALSPL HKIDEKYLFH LKIAGELASM GKILSVYLAH KHSAYFILNA LSYGFSHQDR AIICLLAQFS HKKIPKDNAI AHMSAMMPSL LTLQWLSFIL SLAENLCLTD SHHLKYTLEK NKLVIHSNDA LYLAKEMLPK LVKPIPLTIE FA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta aaatcacaac cgtgattgat ataggctcta attcagtgcg tttggctgtc tttaaaaaga cgagccagtt tgggttttac ttgctttttg agactaagtc taaggttagg atttcagagg gttgttatgc gtttaatgga atcttgcaag aaatccccat gcaaagagcc gttaaagcct tgagcgaatt taaagaaatc gctctcaaat acaaaagcaa aaaaatcttg tgcgtggcga cctcagcggt gcgcgatgcc cctaacagat tagagtttgt agcgagggtg aaaaaggctt gcggtttgca aatcaaaatc attgatgggc aaaaagaagc gctctatggt gggattgcgt gcgcgaattt gttgcataaa aattcaggga tcacgataga tattggaggg ggtagcacgg agtgcgcgtt gattgaaaaa ggcaagatta aggacttaat ctcgcttgat gttgggacga ttcgcattaa agaaatgttt ttagacaaag acttagatgt caaattggct aaagccttta tccaaaaaga agtctctaaa ctgcccttta agcataaaaa cgcctttggg gtggggggga cgattagggc gttgagtaaa gtgctgatga aacgctttga ttaccctatt gattctttgc atggttatga aatagatgca cataaaaatt tagcgttcat tgaaaaaatc gtcatgctca aagaagatca attacggctt ttaggggtga atgaagagcg tttggatagc attaggagtg gggcgttgat tctatcagtc gttttggagc atttaaaaac ttccttgatg atcactagcg gggtgggggt gagagaaggc gtgtttttga gcgatttatt acgaaaccat taccataaat tcccccccaa tatcaacccc tctctcatct ctttaaaaga tcgctttttg ccccatgaaa agcacagcca aaaggtcaaa aaagaatgcg tgaaattgtt tgaagcctta tcgcctttgc ataaaataga tgaaaaatac cttttccatt taaagattgc gggggaatta gcgagcatgg gtaagatttt aagcgtctat ttagcccaca agcacagcgc gtattttatt ttgaacgctt tgagttatgg ctttagccac caggatagag cgatcatttg cttattagcc caattcagcc ataaaaaaat ccctaaagac aacgctatcg cccacatgag tgcgatgatg ccaagccttt taaccttaca atggctgagt tttatccttt ctttagccga aaatttgtgc ctaacagaca gccatcatct aaaatacacg ctagaaaaaa acaagcttgt gatccattct aatgatgcgc tttacttggc taaagagatg ctccctaaac tcgttaagcc cattcctttg acgatagagt ttgcttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat