SSGCID: Target Page

HepyC.17519.a

ADP-heptose-lpsheptosyltransferase II

CENTER ID:	HepyC.17519.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.17519.a.B1.GE41021	full length		1	349

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1236
UniProt:	B5Z8I7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVNAPKRMR ILLRLPNWLG DGVMASSLFY TLKHHYPNAH FILVGPQITC ELFKKDEKIE AVFIDDTKKS FFRLLATYKL AQKIGRCDIA ITLNNHFYSA FFLYATKTPV RIGFAQFFRS LFLSHAIAPA PKEYHQVEKY CFLFSQFLKK ELDQKSVLPL KLAFNLPTHT PNTPKKIGFN PSASYGSAKR WPASYYAEVS AVLLEEGHEI YFFGAKEDTI VSEEILKLIK GSLKNPLLSH NAYNLCGKTS IEELIQRIAI LDLFITNDSG PMHVAASVQT PLIALFGPTD EKETRPYKAQ KAIVLNHHLS CAPCKKRVCP LKNEKNHLCM KSITPLEVLE AAHTLLEKP

NT Sequence

atgagcgtaa atgcacccaa acgcatgcgt attttattgc gtttgcctaa ttggttaggc gatggggtga tggcaagctc gctcttttac acccttaaac accactaccc taacgcgcat tttatcttag tgggcccaca aatcacttgc gaacttttca aaaaagatga aaaaatagaa gccgttttta tagacgacac caaaaaatcc tttttcaggc tgctagccac ttacaaactc gctcaaaaaa tagggcgttg cgatatagcg atcaccttaa acaaccattt ttattccgct ttttttctct atgcgacaaa aacgcccgtt cgcatcggtt ttgctcaatt ttttcgttct ttgtttctca gccatgcgat cgctcctgcc cctaaagagt atcatcaagt ggaaaagtat tgctttttgt tttcgcaatt tttaaaaaaa gaattggatc aaaaaagcgt tttaccctta aaattagcct ttaacctccc cactcacacc ccaaacaccc ctaaaaaaat cggctttaac cctagcgcaa gctatgggag cgctaaaaga tggccagctt cttattacgc tgaagtttct gctgttttgt tagaagaagg gcatgaaatt tatttttttg gggctaaaga agatactatc gtttctgaag aaattttaaa actcatcaaa ggctcattaa aaaacccctt attatcccac aacgcttaca atctgtgcgg gaaaacaagc attgaagaat tgatacaacg catcgcaatt ttagatttat tcatcactaa cgatagcggt cctatgcatg tggctgctag cgtgcaaacc cccttaatcg ctctttttgg ccccactgat gaaaaagaga ctcgccccta taaagctcaa aaagcgatcg tattgaacca ccatttaagc tgtgcacctt gtaaaaagcg agtttgcccc ttaaagaatg aaaaaaacca cttgtgcatg aaatctatca cgccccttga agtcctagaa gccgctcaca ctcttttaga aaagccttaa

Details for HepyC.17519.a.B1.GE41021

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	41kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS VNAPKRMRIL LRLPNWLGDG VMASSLFYTL KHHYPNAHFI LVGPQITCEL FKKDEKIEAV FIDDTKKSFF RLLATYKLAQ KIGRCDIAIT LNNHFYSAFF LYATKTPVRI GFAQFFRSLF LSHAIAPAPK EYHQVEKYCF LFSQFLKKEL DQKSVLPLKL AFNLPTHTPN TPKKIGFNPS ASYGSAKRWP ASYYAEVSAV LLEEGHEIYF FGAKEDTIVS EEILKLIKGS LKNPLLSHNA YNLCGKTSIE ELIQRIAILD LFITNDSGPM HVAASVQTPL IALFGPTDEK ETRPYKAQKA IVLNHHLSCA PCKKRVCPLK NEKNHLCMKS ITPLEVLE

Validated NT Sequence

ggcttctagg acttcaaggg gcgtgataga tttcatgcac aagtggtttt tttcattctt taaggggcaa actcgctttt tacaaggtgc acagcttaaa tggtggttca atacgatcgc tttttgagct ttataggggc gagtctcttt ttcatcagtg gggccaaaaa gagcgattaa gggggtttgc acgctagcag ccacatgcat aggaccgcta tcgttagtga tgaataaatc taaaattgcg atgcgttgta tcaattcttc aatgcttgtt ttcccgcaca gattgtaagc gttgtgggat aataaggggt tttttaatga gcctttgatg agttttaaaa tttcttcaga aacgatagta tcttctttag ccccaaaaaa ataaatttca tgcccttctt ctaacaaaac agcagaaact tcagcgtaat aagaagctgg ccatctttta gcgctcccat agcttgcgct agggttaaag ccgatttttt taggggtgtt tggggtgtga gtggggaggt taaaggctaa ttttaagggt aaaacgcttt tttgatccaa ttcttttttt aaaaattgcg aaaacaaaaa gcaatacttt tccacttgat gatactcttt aggggcagga gcgatcgcat ggctgagaaa caaagaacga aaaaattgag caaaaccgat gcgaacgggc gtttttgtcg catagagaaa aaaagcggaa taaaaatggt tgtttaaggt gatcgctata tcgcaacgcc ctattttttg agcgagtttg taagtggcta gcagcctgaa aaaggatttt ttggtgtcgt ctataaaaac ggcttctatt ttttcatctt ttttgaaaag ttcgcaagtg atttgtgggc ccactaagat aaaatgcgcg ttagggtagt ggtgtttaag ggtgtaaaag agcgagcttg ccatcacccc atcgcctaac caattaggca aacgcaataa aatacgcatg cgtttgggtg catttacgct catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg taaatgcacc caaacgcatg cgtattttat tgcgtttgcc taattggtta ggcgatgggg tgatggcaag ctcgctcttt tacaccctta aacaccacta ccctaacgcg cattttatct tagtgggccc acaaatcact tgcgaacttt tcaaaaaaga tgaaaaaata gaagccgttt ttatagacga caccaaaaaa tcctttttca ggctgctagc cacttacaaa ctcgctcaaa aaatagggcg ttgcgatata gcgatcacct taaacaacca tttttattcc gctttttttc tctatgcgac aaaaacgccc gttcgcatcg gttttgctca attttttcgt tctttgtttc tcagccatgc gatcgctcct gcccctaaag agtatcatca agtggaaaag tattgctttt tgttttcgca atttttaaaa aaagaattgg atcaaaaaag cgttttaccc ttaaaattag cctttaacct ccccactcac accccaaaca cccctaaaaa aatcggcttt aaccctagcg caagctatgg gagcgctaaa agatggccag cttcttatta cgctgaagtt tctgctgttt tgttagaaga agggcatgaa atttattttt ttggggctaa agaagatact atcgtttctg aagaaatttt aaaactcatc aaaggctcat taaaaaaccc cttattatcc cacaacgctt acaatctgtg cgggaaaaca agcattgaag aattgataca acgcatcgca attttagatt tattcatcac taacgatagc ggtcctatgc atgtggctgc tagcgtgcaa acccccttaa tcgctctttt tggccccact gatgaaaaag agactcgccc ctataaagct caaaaagcga tcgtattgaa ccaccattta agctgtgcac cttgtaaaaa gcgagtttgc cccttaaaga atgaaaaaaa ccacttgtgc atgaaatcta tcacgcccct tgaagtccta gaagccgctc acactctttt agaaaagcct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat