HepyC.17194.b

RNA polymerase sigma factor RpoD (Sigma-70)

CENTER ID: HepyC.17194.b
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17194.b.B1.GE41019 full length 1 682
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17194.b.B1.PS38257 full length 1 682

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.94
UniProt: B5Z9M1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKKANEEKA PKRAKQEAKT EATQENKAKE NNKENKNNKA KESKIKENKT KESKIKEAKA KEPIPVKKLS FNEELEELFA NSLSDCVSYE SIIQISAKVP TLAQIKKIKE LCQKYQKKLV SSSEYAKKLN AIDKIKKTEE KQKVLDEELE DGYDFLKEKD FLEWSRSDNP VRMYLREMGD IKLLSKDEEI ELSKQIRLGE DIILDAICSV PYLIDFIYAY KDALINRERR VKELFRSFDD DDENSVSDPK KDDDSEEDEE NEERKKVVSE KDKKRVEKVQ ESFKALDKAK KEWLKALEAP VDEKEDELVR LLTLAYKRQT LKDRLYDLEP TSKLINELVK TMETTLKSGD GFEKELKRLE YKLPLFNDTL IANHKKILAN ITNMTKEDII AQVPEATMVS VYMDLKKLFL TKEASEEGFD LAPNKLKEIL EQIKRGKLIS DRAKNKMAKS NLRLVVSIAK RFTSRGLPFL DLIQEGNIGL MKAVDKFEHE KGFKFSTYAT WWIKQAISRA IADQARTIRI PIHMIDTINR INKVMRKHIQ ENGKEPDLEV VAEEVGLSLD KVKNVIKVTK EPISLETPVG NDDDGKFGDF VEDKNIVSSI DHIMREDLKA QIESVLDQLN EREKAVIRMR FGLLDDESDR TLEEIGKELN VTRERVRQIE SSAIKKLRSP QYGRILRNYL RI
NT Sequence
atgaaaaaga aagctaacga agaaaaagcc cccaaaagag ctaaacagga agccaaaaca gaagccacac aggaaaataa agctaaagaa aataataagg aaaacaaaaa caataaagcc aaagagagca aaattaaaga aaataaaacc aaagaaagca aaattaaaga agctaaagct aaagaaccta tccctgttaa aaagctcagt ttcaatgaag aattagaaga attgttcgcc aattccttaa gcgattgcgt ttcttatgag tctatcattc aaatcagtgc gaaagtcccc actctagccc aaatcaaaaa aatcaaagaa ttgtgccaaa aataccaaaa gaaattggtc agctcttcag aatacgctaa aaaactcaat gcgattgaca agattaaaaa aactgaagaa aagcaaaaag ttttagatga agaattagaa gatggctatg actttttgaa agaaaaggat tttttagagt ggagcagaag cgataacccg gtgcgcatgt atttgcgtga aatgggggat ataaaacttt taagcaaaga tgaagagatt gaattgagca aacaaatccg cttgggcgaa gacattattt tagacgcgat ttgctcggtg ccgtatttga ttgattttat ttatgcgtat aaagacgctt taatcaatcg tgaacgaagg gttaaagagc ttttcaggag ctttgatgat gacgatgaaa atagcgtgag cgatcctaaa aaagatgatg acagcgaaga agacgaagaa aacgaagaaa ggaaaaaagt cgtttctgaa aaagacaaga agcgcgttga aaaggtccaa gagagtttta aagccctaga caaggctaaa aaagaatggc ttaaagccct tgaagcccct gtagatgaaa aagaagatga attggtgcgt ttattgaccc tagcttacaa acgccaaacg ctcaaagaca gactctatga tttagagcct accagcaaac tgattaatga attagtcaaa acgatggaga ccactttaaa aagcggcgat ggatttgaaa aagagttgaa acgcttggaa tacaaactgc ccttattcaa tgacactctg atcgctaacc ataaaaaaat ccttgctaat atcactaaca tgactaaaga agacattatc gctcaagtgc cagaagcgac tatggttagc gtgtatatgg atctcaaaaa actctttttg actaaagaag cgagcgaaga gggctttgat ttagccccta acaagctaaa agaaatttta gagcaaatca aaagagggaa attgatttcc gatcgcgcta aaaacaaaat ggctaaatcc aatttaaggt tggtagtgag catcgctaaa cgattcacga gcagaggctt acccttcttg gatttgattc aagagggcaa tatcggcttg atgaaagcag tggataagtt tgagcatgaa aagggcttca agttttccac ctatgcgacc tggtggatca aacaagctat cagcagagcc atagccgatc aggcccgcac tatccgcatc cctattcaca tgattgacac catcaatcgc atcaataaag tcatgcgcaa acacattcaa gaaaacggca aagagcctga tttagaagtg gtggctgaag aagtggggct ttccttagat aaagtgaaga atgtgattaa ggttactaaa gagcctatca gcttggaaac cccagttggc aatgatgatg acggcaaatt tggggatttt gtggaagaca aaaatatcgt tagctccatt gatcacatca tgcgagaaga tttgaaagcc caaattgaaa gcgttttgga tcagttgaat gagcgagaaa aagcggtgat ccgcatgcgt tttgggcttt tggacgatga aagcgatcgg actttagaag aaatcggtaa ggaattgaat gttactagag aaagggtgcg ccagattgaa agctctgcga ttaaaaaatt gagaagcccg caatacgggc gcattttaag aaattatttg cgcatttga
Details for HepyC.17194.b.B1.GE41019
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 80kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK KKANEEKAPK RAKQEAKTEA TQENKAKENN KENKNNKAKE SKIKENKTKE SKIKEAKAKE PIPVKKLSFN EELEELFANS LSDCVSYESI IQISAKVPTL AQIKKIKELC QKYQKKLVSS SEYAKKLNAI DKIKKTEEKQ KVLDEELEDG YDFLKEKDFL EWSRSDNPVR MYLREMGDIK LLSKDEEIEL SKQIRLGEDI ILDAICSVPY LIDFIYAYKD ALINRERRVK ELFRSFDDDD ENSVSDPKKD DDSEEDEENE ERKKVVSEKD KKRVEKVQES FKALDKAKKE WLKALEAPVD EKEDELVRLL TLAYKRQTLK DRLYDLEPTS KLINELVKTM ETTLKSGDGF EKELKRLEYK LPLFNDTLIA NHKKILANIT NMTKEDIIAQ VPEATMVSVY MDLKKLFLTK EASEEGFDLA PNKLKEILEQ IKRGKLISDR AKNKMAKSNL RLVVSIAKRF TSRGLPFLDL IQEGNIGLMK AVDKFEHEKG FKFSTYATWW IKQAISRAIA DQARTIRIPI HMIDTINRIN KVMRKHIQEN GKEPDLEVVA EEVGLSLDKV KNVIKVTKEP ISLETPVGND DDGKFGDFVE DKNIVSSIDH IMREDLKAQI ESVLDQLNER EKAVIRMRFG LLDDESDRTL EEIGKELNVT RERVRQIESS AIKKLRSPQY GRILRNYLRI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa agaaagctaa cgaagaaaaa gcccccaaaa gagctaaaca ggaagccaaa acagaagcca cacaggaaaa taaagctaaa gaaaataata aggaaaacaa aaacaataaa gccaaagaga gcaaaattaa agaaaataaa accaaagaaa gcaaaattaa agaagctaaa gctaaagaac ctatccctgt taaaaagctc agtttcaatg aagaattaga agaattgttc gccaattcct taagcgattg cgtttcttat gagtctatca ttcaaatcag tgcgaaagtc cccactctag cccaaatcaa aaaaatcaaa gaattgtgcc aaaaatacca aaagaaattg gtcagctctt cagaatacgc taaaaaactc aatgcgattg acaagattaa aaaaactgaa gaaaagcaaa aagttttaga tgaagaatta gaagatggct atgacttttt gaaagaaaag gattttttag agtggagcag aagcgataac ccggtgcgca tgtatttgcg tgaaatgggg gatataaaac ttttaagcaa agatgaagag attgaattga gcaaacaaat ccgcttgggc gaagacatta ttttagacgc gatttgctcg gtgccgtatt tgattgattt tatttatgcg tataaagacg ctttaatcaa tcgtgaacga agggttaaag agcttttcag gagctttgat gatgacgatg aaaatagcgt gagcgatcct aaaaaagatg atgacagcga agaagacgaa gaaaacgaag aaaggaaaaa agtcgtttct gaaaaagaca agaagcgcgt tgaaaaggtc caagagagtt ttaaagccct agacaaggct aaaaaagaat ggcttaaagc ccttgaagcc cctgtagatg aaaaagaaga tgaattggtg cgtttattga ccctagctta caaacgccaa acgctcaaag acagactcta tgatttagag cctaccagca aactgattaa tgaattagtc aaaacgatgg agaccacttt aaaaagcggc gatggatttg aaaaagagtt gaaacgcttg gaatacaaac tgcccttatt caatgacact ctgatcgcta accataaaaa aatccttgct aatatcacta acatgactaa agaagacatt atcgctcaag tgccagaagc gactatggtt agcgtgtata tggatctcaa aaaactcttt ttgactaaag aagcgagcga agagggcttt gatttagccc ctaacaagct aaaagaaatt ttagagcaaa tcaaaagagg gaaattgatt tccgatcgcg ctaaaaacaa aatggctaaa tccaatttaa ggttggtagt gagcatcgct aaacgattca cgagcagagg cttacccttc ttggatttga ttcaagaggg caatatcggc ttgatgaaag cagtggataa gtttgagcat gaaaagggct tcaagttttc cacctatgcg acctggtgga tcaaacaagc tatcagcaga gccatagccg atcaggcccg cactatccgc atccctattc acatgattga caccatcaat cgcatcaata aagtcatgcg caaacacatt caagaaaacg gcaaagagcc tgatttagaa gtggtggctg aagaagtggg gctttcctta gataaagtga agaatgtgat taaggttact aaagagccta tcagcttgga aaccccagtt ggcaatgatg atgacggcaa atttggggat tttgtggaag acaaaaatat cgttagctcc attgatcaca tcatgcgaga agatttgaaa gcccaaattg aaagcgtttt ggatcagttg aatgagcgag aaaaagcggt gatccgcatg cgttttgggc ttttggacga tgaaagcgat cggactttag aagaaatcgg taaggaattg aatgttacta gagaaagggt gcgccagatt gaaagctctg cgattaaaaa attgagaagc ccgcaatacg ggcgcatttt aagaaattat ttgcgcattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for HepyC.17194.b.B1.PS38257
PURIFICATION DATe: 4/12/2017
CONCENTRATION: 12.3mg/ml
OBSERVED MW: 82kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK KKANEEKAPK RAKQEAKTEA TQENKAKENN KENKNNKAKE SKIKENKTKE SKIKEAKAKE PIPVKKLSFN EELEELFANS LSDCVSYESI IQISAKVPTL AQIKKIKELC QKYQKKLVSS SEYAKKLNAI DKIKKTEEKQ KVLDEELEDG YDFLKEKDFL EWSRSDNPVR MYLREMGDIK LLSKDEEIEL SKQIRLGEDI ILDAICSVPY LIDFIYAYKD ALINRERRVK ELFRSFDDDD ENSVSDPKKD DDSEEDEENE ERKKVVSEKD KKRVEKVQES FKALDKAKKE WLKALEAPVD EKEDELVRLL TLAYKRQTLK DRLYDLEPTS KLINELVKTM ETTLKSGDGF EKELKRLEYK LPLFNDTLIA NHKKILANIT NMTKEDIIAQ VPEATMVSVY MDLKKLFLTK EASEEGFDLA PNKLKEILEQ IKRGKLISDR AKNKMAKSNL RLVVSIAKRF TSRGLPFLDL IQEGNIGLMK AVDKFEHEKG FKFSTYATWW IKQAISRAIA DQARTIRIPI HMIDTINRIN KVMRKHIQEN GKEPDLEVVA EEVGLSLDKV KNVIKVTKEP ISLETPVGND DDGKFGDFVE DKNIVSSIDH IMREDLKAQI ESVLDQLNER EKAVIRMRFG LLDDESDRTL EEIGKELNVT RERVRQIESS AIKKLRSPQY GRILRNYLRI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA AGAAAGCTAA CGAAGAAAAA GCCCCCAAAA GAGCTAAACA GGAAGCCAAA ACAGAAGCCA CACAGGAAAA TAAAGCTAAA GAAAATAATA AGGAAAACAA AAACAATAAA GCCAAAGAGA GCAAAATTAA AGAAAATAAA ACCAAAGAAA GCAAAATTAA AGAAGCTAAA GCTAAAGAAC CTATCCCTGT TAAAAAGCTC AGTTTCAATG AAGAATTAGA AGAATTGTTC GCCAATTCCT TAAGCGATTG CGTTTCTTAT GAGTCTATCA TTCAAATCAG TGCGAAAGTC CCCACTCTAG CCCAAATCAA AAAAATCAAA GAATTGTGCC AAAAATACCA AAAGAAATTG GTCAGCTCTT CAGAATACGC TAAAAAACTC AATGCGATTG ACAAGATTAA AAAAACTGAA GAAAAGCAAA AAGTTTTAGA TGAAGAATTA GAAGATGGCT ATGACTTTTT GAAAGAAAAG GATTTTTTAG AGTGGAGCAG AAGCGATAAC CCGGTGCGCA TGTATTTGCG TGAAATGGGG GATATAAAAC TTTTAAGCAA AGATGAAGAG ATTGAATTGA GCAAACAAAT CCGCTTGGGC GAAGACATTA TTTTAGACGC GATTTGCTCG GTGCCGTATT TGATTGATTT TATTTATGCG TATAAAGACG CTTTAATCAA TCGTGAACGA AGGGTTAAAG AGCTTTTCAG GAGCTTTGAT GATGACGATG AAAATAGCGT GAGCGATCCT AAAAAAGATG ATGACAGCGA AGAAGACGAA GAAAACGAAG AAAGGAAAAA AGTCGTTTCT GAAAAAGACA AGAAGCGCGT TGAAAAGGTC CAAGAGAGTT TTAAAGCCCT AGACAAGGCT AAAAAAGAAT GGCTTAAAGC CCTTGAAGCC CCTGTAGATG AAAAAGAAGA TGAATTGGTG CGTTTATTGA CCCTAGCTTA CAAACGCCAA ACGCTCAAAG ACAGACTCTA TGATTTAGAG CCTACCAGCA AACTGATTAA TGAATTAGTC AAAACGATGG AGACCACTTT AAAAAGCGGC GATGGATTTG AAAAAGAGTT GAAACGCTTG GAATACAAAC TGCCCTTATT CAATGACACT CTGATCGCTA ACCATAAAAA AATCCTTGCT AATATCACTA ACATGACTAA AGAAGACATT ATCGCTCAAG TGCCAGAAGC GACTATGGTT AGCGTGTATA TGGATCTCAA AAAACTCTTT TTGACTAAAG AAGCGAGCGA AGAGGGCTTT GATTTAGCCC CTAACAAGCT AAAAGAAATT TTAGAGCAAA TCAAAAGAGG GAAATTGATT TCCGATCGCG CTAAAAACAA AATGGCTAAA TCCAATTTAA GGTTGGTAGT GAGCATCGCT AAACGATTCA CGAGCAGAGG CTTACCCTTC TTGGATTTGA TTCAAGAGGG CAATATCGGC TTGATGAAAG CAGTGGATAA GTTTGAGCAT GAAAAGGGCT TCAAGTTTTC CACCTATGCG ACCTGGTGGA TCAAACAAGC TATCAGCAGA GCCATAGCCG ATCAGGCCCG CACTATCCGC ATCCCTATTC ACATGATTGA CACCATCAAT CGCATCAATA AAGTCATGCG CAAACACATT CAAGAAAACG GCAAAGAGCC TGATTTAGAA GTGGTGGCTG AAGAAGTGGG GCTTTCCTTA GATAAAGTGA AGAATGTGAT TAAGGTTACT AAAGAGCCTA TCAGCTTGGA AACCCCAGTT GGCAATGATG ATGACGGCAA ATTTGGGGAT TTTGTGGAAG ACAAAAATAT CGTTAGCTCC ATTGATCACA TCATGCGAGA AGATTTGAAA GCCCAAATTG AAAGCGTTTT GGATCAGTTG AATGAGCGAG AAAAAGCGGT GATCCGCATG CGTTTTGGGC TTTTGGACGA TGAAAGCGAT CGGACTTTAG AAGAAATCGG TAAGGAATTG AATGTTACTA GAGAAAGGGT GCGCCAGATT GAAAGCTCTG CGATTAAAAA ATTGAGAAGC CCGCAATACG GGCGCATTTT AAGAAATTAT TTGCGCATTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT