SSGCID: Target Page

HepyC.17194.a

Uncharacterized protein

CENTER ID:	HepyC.17194.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.17194.a.B2.GE41018	mature protein		20	415

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.17194.a.B2.PS38246	mature protein		20	415

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.665
UniProt:	B5Z732

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRKIFSYVLK ALLFIGIVYA EPESKVEALE GRKQESSLDK KIRQELKNKD LKNKKEEKKN TEEKKETKAK KKPRAEVHHG DAKNPTQKIT PPKIKEGAKG VQNQGTQNQG VQNNAPKLEE KETTSQTLEK NKGTSPSSQF NSIFGNPNNA ANNTLEDKVV GGISLLVNGS PITLYQIQEE QKKSKVSKAQ ARDRLIAERI KNQEIERLKI HVDDDKLDQE MAMMAQQQGM DLDHFKQMLM AEGHYKLYRD QLKEHLEMQE LLRNILLTNV DTSSETKMRE YYNKHKEQFS IPTEIETVRY TSTSQEDLER AMADPNLEIP GVSKANEKIE MKTLNPQIAQ VFISHEQGSF TPVMNGGGGQ FITFYIKEKK GKNEVSFSQA KQFIAQKLVE ESKDKILEEH FEKLRVKSRI VMIRE

NT Sequence

atgaggaaaa ttttttctta tgttttgaag gctttgttgt ttattgggat cgtttatgca gagccggaat ctaaagtgga agccttagaa gggaggaagc aagagtcttc tttggataaa aaaatccgcc aagaattgaa gaataaggat ttgaaaaata aaaaagaaga aaagaaaaac accgaagaaa agaaagaaac aaaagccaaa aaaaagccca gagcagaagt ccatcatggg gatgccaaaa atcctactca aaaaataacg cctcctaaaa tcaaagaggg cgctaaaggg gttcaaaatc aaggcacgca aaatcaaggc gttcaaaata acgcgccaaa acttgaagaa aaagagacaa cctctcaaac tcttgaaaaa aataagggga caagccctag ctctcaattc aattctattt ttggtaatcc taataacgct gctaacaaca cccttgaaga taaggtcgta gggggcattt ctttgcttgt taatggctcg cctatcacgc tgtatcaaat ccaagaagag caaaaaaaat ctaaagtgag caaagctcaa gccagggatc gtttgattgc ggagcgcatt aaaaaccaag aaattgagcg cttaaaaatc catgtagatg atgacaagct agaccaagaa atggcgatga tggcgcaaca gcaaggcatg gatttagacc atttcaaaca aatgcttatg gctgaggggc attataaact ctatagagat cagcttaagg agcatttaga aatgcaagaa ttgttgcgta atatcttgct cacgaatgtg gataccagct ctgaaaccaa aatgcgcgaa tattacaaca aacacaagga gcaattcagt atccccactg aaatagaaac cgtgcgctac acttcaacga gtcaagaaga tttagaaagg gctatggcag accctaattt agaaattcca ggggtgagta aggccaatga aaaaatagag atgaaaaccc taaaccctca aatcgctcaa gtctttattt cgcatgagca aggctctttc acgcccgtta tgaatggggg tggggggcag ttcatcactt tttatatcaa agaaaaaaag ggtaaaaacg aagtgagttt cagtcaggcc aagcaattca tcgcccaaaa attagtggaa gaatctaaag ataagatttt agaagagcat tttgaaaaat tgcgcgtcaa gtctaggatt gtgatgatta gagagtga

Details for HepyC.17194.a.B2.GE41018

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA EPESKVEALE GRKQESSLDK KIRQELKNKD LKNKKEEKKN TEEKKETKAK KKPRAEVHHG DAKNPTQKIT PPKIKEGAKG VQNQGTQNQG VQNNAPKLEE KETTSQTLEK NKGTSPSSQF NSIFGNPNNA ANNTLEDKVV GGISLLVNGS PITLYQIQEE QKKSKVSKAQ ARDRLIAERI KNQEIERLKI HVDDDKLDQE MAMMAQQQGM DLDHFKQMLM AEGHYKLYRD QLKEHLEMQE LLRNILLTNV DTSSETKMRE YYNKHKEQFS IPTEIETVRY TSTSQEDLER AMADPNLEIP GVSKANEKIE MKTLNPQIAQ VFISHEQGSF TPVMNGGGGQ FITFYIKEKK GKNEVSFSQA KQFIAQKLVE ESKDKILEEH FEKLRVK

Validated NT Sequence

acttgacgcg caatttttca aaatgctctt ctaaaatctt atctttagat tcttccacta atttttgggc gatgaattgc ttggcctgac tgaaactcac ttcgttttta cccttttttt ctttgatata aaaagtgatg aactgccccc cacccccatt cataacgggc gtgaaagagc cttgctcatg cgaaataaag acttgagcga tttgagggtt tagggttttc atctctattt tttcattggc cttactcacc cctggaattt ctaaattagg gtctgccata gccctttcta aatcttcttg actcgttgaa gtgtagcgca cggtttctat ttcagtgggg atactgaatt gctccttgtg tttgttgtaa tattcgcgca ttttggtttc agagctggta tccacattcg tgagcaagat attacgcaac aattcttgca tttctaaatg ctccttaagc tgatctctat agagtttata atgcccctca gccataagca tttgtttgaa atggtctaaa tccatgcctt gctgttgcgc catcatcgcc atttcttggt ctagcttgtc atcatctaca tggattttta agcgctcaat ttcttggttt ttaatgcgct ccgcaatcaa acgatccctg gcttgagctt tgctcacttt agattttttt tgctcttctt ggatttgata cagcgtgata ggcgagccat taacaagcaa agaaatgccc cctacgacct tatcttcaag ggtgttgtta gcagcgttat taggattacc aaaaatagaa ttgaattgag agctagggct tgtcccctta tttttttcaa gagtttgaga ggttgtctct ttttcttcaa gttttggcgc gttattttga acgccttgat tttgcgtgcc ttgattttga acccctttag cgccctcttt gattttagga ggcgttattt tttgagtagg atttttggca tccccatgat ggacttctgc tctgggcttt tttttggctt ttgtttcttt cttttcttcg gtgtttttct tttcttcttt tttatttttc aaatccttat tcttcaattc ttggcggatt tttttatcca aagaagactc ttgcttcctc ccttctaagg cttccacttt agattccggc tctgccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHAE PESKVEALEG RKQESSLDKK IRQELKNKDL KNKKEEKKNT EEKKETKAKK KPRAEVHHGD AKNPTQKITP PKIKEGAKGV QNQGTQNQGV QNNAPKLEEK ETTSQTLEKN KGTSPSSQFN SIFGNPNNAA NNTLEDKVVG GISLLVNGSP ITLYQIQEEQ KKSKVSKAQA RDRLIAERIK NQEIERLKIH VDDDKLDQEM AMMAQQQGMD LDHFKQMLMA EGHYKLYRDQ LKEHLEMQEL LRNILLTNVD TSSETKMREY YNKHKEQFSI PTEIETVRYT STSQEDLERA MADPNLEIPG VSKANEKIEM KTLNPQIAQV FISHEQGSFT PVMNGGGGQF ITFYIKEKKG KNEVSFSQAK QFIAQKLVEE SKDKILEEHF EKLRVKSRIV MIRE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagagc cggaatctaa agtggaagcc ttagaaggga ggaagcaaga gtcttctttg gataaaaaaa tccgccaaga attgaagaat aaggatttga aaaataaaaa agaagaaaag aaaaacaccg aagaaaagaa agaaacaaaa gccaaaaaaa agcccagagc agaagtccat catggggatg ccaaaaatcc tactcaaaaa ataacgcctc ctaaaatcaa agagggcgct aaaggggttc aaaatcaagg cacgcaaaat caaggcgttc aaaataacgc gccaaaactt gaagaaaaag agacaacctc tcaaactctt gaaaaaaata aggggacaag ccctagctct caattcaatt ctatttttgg taatcctaat aacgctgcta acaacaccct tgaagataag gtcgtagggg gcatttcttt gcttgttaat ggctcgccta tcacgctgta tcaaatccaa gaagagcaaa aaaaatctaa agtgagcaaa gctcaagcca gggatcgttt gattgcggag cgcattaaaa accaagaaat tgagcgctta aaaatccatg tagatgatga caagctagac caagaaatgg cgatgatggc gcaacagcaa ggcatggatt tagaccattt caaacaaatg cttatggctg aggggcatta taaactctat agagatcagc ttaaggagca tttagaaatg caagaattgt tgcgtaatat cttgctcacg aatgtggata ccagctctga aaccaaaatg cgcgaatatt acaacaaaca caaggagcaa ttcagtatcc ccactgaaat agaaaccgtg cgctacactt caacgagtca agaagattta gaaagggcta tggcagaccc taatttagaa attccagggg tgagtaaggc caatgaaaaa atagagatga aaaccctaaa ccctcaaatc gctcaagtct ttatttcgca tgagcaaggc tctttcacgc ccgttatgaa tgggggtggg gggcagttca tcacttttta tatcaaagaa aaaaagggta aaaacgaagt gagtttcagt caggccaagc aattcatcgc ccaaaaatta gtggaagaat ctaaagataa gattttagaa gagcattttg aaaaattgcg cgtcaagtct aggattgtga tgattagaga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for HepyC.17194.a.B2.PS38246

PURIFICATION DATe:	3/30/2017
CONCENTRATION:	22.4mg/ml
OBSERVED MW:	54kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCAGAGC CGGAATCTAA AGTGGAAGCC TTAGAAGGGA GGAAGCAAGA GTCTTCTTTG GATAAAAAAA TCCGCCAAGA ATTGAAGAAT AAGGATTTGA AAAATAAAAA AGAAGAAAAG AAAAACACCG AAGAAAAGAA AGAAACAAAA GCCAAAAAAA AGCCCAGAGC AGAAGTCCAT CATGGGGATG CCAAAAATCC TACTCAAAAA ATAACGCCTC CTAAAATCAA AGAGGGCGCT AAAGGGGTTC AAAATCAAGG CACGCAAAAT CAAGGCGTTC AAAATAACGC GCCAAAACTT GAAGAAAAAG AGACAACCTC TCAAACTCTT GAAAAAAATA AGGGGACAAG CCCTAGCTCT CAATTCAATT CTATTTTTGG TAATCCTAAT AACGCTGCTA ACAACACCCT TGAAGATAAG GTCGTAGGGG GCATTTCTTT GCTTGTTAAT GGCTCGCCTA TCACGCTGTA TCAAATCCAA GAAGAGCAAA AAAAATCTAA AGTGAGCAAA GCTCAAGCCA GGGATCGTTT GATTGCGGAG CGCATTAAAA ACCAAGAAAT TGAGCGCTTA AAAATCCATG TAGATGATGA CAAGCTAGAC CAAGAAATGG CGATGATGGC GCAACAGCAA GGCATGGATT TAGACCATTT CAAACAAATG CTTATGGCTG AGGGGCATTA TAAACTCTAT AGAGATCAGC TTAAGGAGCA TTTAGAAATG CAAGAATTGT TGCGTAATAT CTTGCTCACG AATGTGGATA CCAGCTCTGA AACCAAAATG CGCGAATATT ACAACAAACA CAAGGAGCAA TTCAGTATCC CCACTGAAAT AGAAACCGTG CGCTACACTT CAACGAGTCA AGAAGATTTA GAAAGGGCTA TGGCAGACCC TAATTTAGAA ATTCCAGGGG TGAGTAAGGC CAATGAAAAA ATAGAGATGA AAACCCTAAA CCCTCAAATC GCTCAAGTCT TTATTTCGCA TGAGCAAGGC TCTTTCACGC CCGTTATGAA TGGGGGTGGG GGGCAGTTCA TCACTTTTTA TATCAAAGAA AAAAAGGGTA AAAACGAAGT GAGTTTCAGT CAGGCCAAGC AATTCATCGC CCAAAAATTA GTGGAAGAAT CTAAAGATAA GATTTTAGAA GAGCATTTTG AAAAATTGCG CGTCAAGTCT AGGATTGTGA TGATTAGAGA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT