HepyC.17127.a

Glucose-6-phosphate isomerase (GPI) (EC 5.3.1.9) (Phosphoglucose isomerase) (PGI) (Phosphohexose isomerase) (PHI)

CENTER ID: HepyC.17127.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17127.a.B1.GE41017 full length 1 545
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.17127.a.B1.PS38255 full length 1 545

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1208
UniProt: B5Z8G1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLTQLKTYPK LLKHYEEIKE VHMHDWFSKD KERASRYFVQ FESLSLDYSK NRLNDTTLKL LFELADDCSL KEKIEAMFKG EKINTTEKRA VLHTALRSLN DAEILLDNME VLKSVRSVLK RMRAFSDSVR SGKRLGYTNQ VITDIVNIGI GGSDLGALMV CTALKRYGHP RLKMHFVSNV DGTQILDVLE KINPASTLFI VASKTFSTQE TLTNALTARK WFVERSGDEK HIAKHFVAVS TNKEAVQQFG IDEHNMFEFW DFVGGRYSLW SAIGLSIMIY LGKKNFNALL KGAYLMDEHF RNAPFESNLP VLMGLIGVWY INFFQSKSHL IAPYDQYLRH FPKFIQQLDM ESNGKRISKK GETIPYDTCP VVWGDMGINA QHAFFQLLHQ GTHLIPIDFI ASLDKKPNAK GHHEILFSNV LAQAQAFMKG KSYEEALGEL LFKGLDKDEA KDLAHHRVFF GNRPSNILLL EKISPSNIGA LVALYEHKVF VQGVIWDINS FDQWGVELGK ELAVPILQEL EGHKSNAYFD SSTKHLIELY KNYNQ
NT Sequence
atgctaaccc aattaaaaac ttatccaaaa ttactcaaac attatgaaga aattaaggaa gtgcacatgc acgattggtt ttctaaagac aaagagcgag cgagccgtta tttcgtgcaa tttgaaagct tgagcttgga ttattccaaa aaccgcttga acgataccac tttaaagctt ctttttgaat tagcggatga ttgctcttta aaagaaaaga ttgaagcgat gtttaagggc gaaaaaatca acaccaccga aaaaagggcc gttttacaca ccgccttaag aagcttgaat gacgcagaaa tcttactaga caacatggaa gtgttaaaaa gcgttcggag cgttttaaaa cgcatgcgag cctttagcga tagcgtgagg agcggtaaaa gactgggcta taccaatcaa gtgatcaccg atattgtcaa tatcggcatt ggggggtcag atttaggcgc tttaatggtt tgcaccgccc taaaacgcta cggccacccg agattaaaaa tgcattttgt gtctaatgtg gatggcacgc agattttaga tgttttggaa aaaatcaatc cggccagcac gctttttatc gtggcctcta agactttttc cactcaagaa accttaacca acgccctaac cgctagaaaa tggtttgtag aaagaagcgg cgatgaaaag catatcgcta agcactttgt agcggtatcc accaacaaag aagccgtgca acaatttggc attgacgagc ataacatgtt tgaattttgg gattttgtag gggggcgtta tagcttgtgg tcagctattg gcttatccat tatgatctat ttagggaaga aaaattttaa cgctcttttg aagggggcgt atttgatgga tgagcatttt agaaacgccc cttttgaaag caatttaccc gttttaatgg ggctaattgg cgtgtggtat atcaattttt tccaatccaa aagccacttg atcgctcctt atgatcagta tttgaggcat ttccctaaat tcattcagca attggatatg gaaagtaatg gcaaacgcat cagcaaaaaa ggcgaaacca tcccctatga cacatgccct gttgtttggg gcgatatggg cattaacgct cagcacgcct ttttccagct cttgcatcaa ggtacgcatt taatccccat tgattttatc gcttccttag ataaaaagcc taacgctaaa ggccaccatg agattttatt cagcaatgtt ttagcgcaag cgcaagcctt catgaaaggc aagagttatg aagaagcgct tggggaattg ctctttaaag gcttagacaa agatgaagcc aaagacttgg cccaccacag ggtgtttttt ggcaaccgcc cctctaatat ccttttatta gaaaagattt caccaagcaa tattggggcg ttggtggctc tttatgagca taaggtcttt gtgcaagggg tcatttggga tattaacagc tttgatcaat ggggcgtgga gcttgggaag gaattggccg tgccgatttt acaagaatta gaagggcata aaagcaacgc ttattttgac agctccacta agcatttaat agaattgtat aagaattata accaatag
Details for HepyC.17127.a.B1.GE41017
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 63kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML TQLKTYPKLL KHYEEIKEVH MHDWFSKDKE RASRYFVQFE SLSLDYSKNR LNDTTLKLLF ELADDCSLKE KIEAMFKGEK INTTEKRAVL HTALRSLNDA EILLDNMEVL KSVRSVLKRM RAFSDSVRSG KRLGYTNQVI TDIVNIGIGG SDLGALMVCT ALKRYGHPRL KMHFVSNVDG TQILDVLEKI NPASTLFIVA SKTFSTQETL TNALTARKWF VERSGDEKHI AKHFVAVSTN KEAVQQFGID EHNMFEFWDF VGGRYSLWSA IGLSIMIYLG KKNFNALLKG AYLMDEHFRN APFESNLPVL MGLIGVWYIN FFQSKSHLIA PYDQYLRHFP KFIQQLDMES NGKRISKKGE TIPYDTCPVV WGDMGINAQH AFFQLLHQGT HLIPIDFIAS LDKKPNAKGH HEILFSNVLA QAQAFMKGKS YEEALGELLF KGLDKDEAKD LAHHRVFFGN RPSNILLLEK ISPSNIGALV ALYEHKVFVQ GVIWDINSFD QWGVELGKEL AVPILQELEG HKSNAYFDSS TKHLIELYKN YNQ
Validated NT Sequence
atggctcacc accaccacca ccatatgcta acccaattaa aaacttatcc aaaattactc aaacattatg aagaaattaa ggaagtgcac atgcacgatt ggttttctaa agacaaagag cgagcgagcc gttatttcgt gcaatttgaa agcttgagct tggattattc caaaaaccgc ttgaacgata ccactttaaa gcttcttttt gaattagcgg atgattgctc tttaaaagaa aagattgaag cgatgtttaa gggcgaaaaa atcaacacca ccgaaaaaag ggccgtttta cacaccgcct taagaagctt gaatgacgca gaaatcttac tagacaacat ggaagtgtta aaaagcgttc ggagcgtttt aaaacgcatg cgagccttta gcgatagcgt gaggagcggt aaaagactgg gctataccaa tcaagtgatc accgatattg tcaatatcgg cattgggggg tcagatttag gcgctttaat ggtttgcacc gccctaaaac gctacggcca cccgagatta aaaatgcatt ttgtgtctaa tgtggatggc acgcagattt tagatgtttt ggaaaaaatc aatccggcca gcacgctttt tatcgtggcc tctaagactt tttccactca agaaacctta accaacgccc taaccgctag aaaatggttt gtagaaagaa gcggcgatga aaagcatatc gctaagcact ttgtagcggt atccaccaac aaagaagccg tgcaacaatt tggcattgac gagcataaca tgtttgaatt ttgggatttt gtaggggggc gttatagctt gtggtcagct attggcttat ccattatgat ctatttaggg aagaaaaatt ttaacgctct tttgaagggg gcgtatttga tggatgagca ttttagaaac gccccttttg aaagcaattt acccgtttta atggggctaa ttggcgtgtg gtatatcaat tttttccaat ccaaaagcca cttgatcgct ccttatgatc agtatttgag gcatttccct aaattcattc agcaattgga tatggaaagt aatggcaaac gcatcagcaa aaaaggcgaa accatcccct atgacacatg ccctgttgtt tggggcgata tgggcattaa cgctcagcac gcctttttcc agctcttgca tcaaggtacg catttaatcc ccattgattt tatcgcttcc ttagataaaa agcctaacgc taaaggccac catgagattt tattcagcaa tgttttagcg caagcgcaag ccttcatgaa aggcaagagt tatgaagaag cgcttgggga attgctcttt aaaggcttag acaaagatga agccaaagac ttggcccacc acagggtgtt ttttggcaac cgcccctcta atatcctttt attagaaaag atttcaccaa gcaatattgg ggcgttggtg gctctttatg agcataaggt ctttgtgcaa ggggtcattt gggatattaa cagctttgat caatggggcg tggagcttgg gaaggaattg gccgtgccga ttttacaaga attagaaggg cataaaagca acgcttattt tgacagctcc actaagcatt taatagaatt gtataagaat tataaccaat aa
Expected Protein Sequence
MAHHHHHHML TQLKTYPKLL KHYEEIKEVH MHDWFSKDKE RASRYFVQFE SLSLDYSKNR LNDTTLKLLF ELADDCSLKE KIEAMFKGEK INTTEKRAVL HTALRSLNDA EILLDNMEVL KSVRSVLKRM RAFSDSVRSG KRLGYTNQVI TDIVNIGIGG SDLGALMVCT ALKRYGHPRL KMHFVSNVDG TQILDVLEKI NPASTLFIVA SKTFSTQETL TNALTARKWF VERSGDEKHI AKHFVAVSTN KEAVQQFGID EHNMFEFWDF VGGRYSLWSA IGLSIMIYLG KKNFNALLKG AYLMDEHFRN APFESNLPVL MGLIGVWYIN FFQSKSHLIA PYDQYLRHFP KFIQQLDMES NGKRISKKGE TIPYDTCPVV WGDMGINAQH AFFQLLHQGT HLIPIDFIAS LDKKPNAKGH HEILFSNVLA QAQAFMKGKS YEEALGELLF KGLDKDEAKD LAHHRVFFGN RPSNILLLEK ISPSNIGALV ALYEHKVFVQ GVIWDINSFD QWGVELGKEL AVPILQELEG HKSNAYFDSS TKHLIELYKN YNQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctaa cccaattaaa aacttatcca aaattactca aacattatga agaaattaag gaagtgcaca tgcacgattg gttttctaaa gacaaagagc gagcgagccg ttatttcgtg caatttgaaa gcttgagctt ggattattcc aaaaaccgct tgaacgatac cactttaaag cttctttttg aattagcgga tgattgctct ttaaaagaaa agattgaagc gatgtttaag ggcgaaaaaa tcaacaccac cgaaaaaagg gccgttttac acaccgcctt aagaagcttg aatgacgcag aaatcttact agacaacatg gaagtgttaa aaagcgttcg gagcgtttta aaacgcatgc gagcctttag cgatagcgtg aggagcggta aaagactggg ctataccaat caagtgatca ccgatattgt caatatcggc attggggggt cagatttagg cgctttaatg gtttgcaccg ccctaaaacg ctacggccac ccgagattaa aaatgcattt tgtgtctaat gtggatggca cgcagatttt agatgttttg gaaaaaatca atccggccag cacgcttttt atcgtggcct ctaagacttt ttccactcaa gaaaccttaa ccaacgccct aaccgctaga aaatggtttg tagaaagaag cggcgatgaa aagcatatcg ctaagcactt tgtagcggta tccaccaaca aagaagccgt gcaacaattt ggcattgacg agcataacat gtttgaattt tgggattttg taggggggcg ttatagcttg tggtcagcta ttggcttatc cattatgatc tatttaggga agaaaaattt taacgctctt ttgaaggggg cgtatttgat ggatgagcat tttagaaacg ccccttttga aagcaattta cccgttttaa tggggctaat tggcgtgtgg tatatcaatt ttttccaatc caaaagccac ttgatcgctc cttatgatca gtatttgagg catttcccta aattcattca gcaattggat atggaaagta atggcaaacg catcagcaaa aaaggcgaaa ccatccccta tgacacatgc cctgttgttt ggggcgatat gggcattaac gctcagcacg cctttttcca gctcttgcat caaggtacgc atttaatccc cattgatttt atcgcttcct tagataaaaa gcctaacgct aaaggccacc atgagatttt attcagcaat gttttagcgc aagcgcaagc cttcatgaaa ggcaagagtt atgaagaagc gcttggggaa ttgctcttta aaggcttaga caaagatgaa gccaaagact tggcccacca cagggtgttt tttggcaacc gcccctctaa tatcctttta ttagaaaaga tttcaccaag caatattggg gcgttggtgg ctctttatga gcataaggtc tttgtgcaag gggtcatttg ggatattaac agctttgatc aatggggcgt ggagcttggg aaggaattgg ccgtgccgat tttacaagaa ttagaagggc ataaaagcaa cgcttatttt gacagctcca ctaagcattt aatagaattg tataagaatt ataaccaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for HepyC.17127.a.B1.PS38255
PURIFICATION DATe: 4/12/2017
CONCENTRATION: 24.1mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC buffer: 20mM HEPES pH 7.0, 300mM NaCl,5% glycerol, 1mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML TQLKTYPKLL KHYEEIKEVH MHDWFSKDKE RASRYFVQFE SLSLDYSKNR LNDTTLKLLF ELADDCSLKE KIEAMFKGEK INTTEKRAVL HTALRSLNDA EILLDNMEVL KSVRSVLKRM RAFSDSVRSG KRLGYTNQVI TDIVNIGIGG SDLGALMVCT ALKRYGHPRL KMHFVSNVDG TQILDVLEKI NPASTLFIVA SKTFSTQETL TNALTARKWF VERSGDEKHI AKHFVAVSTN KEAVQQFGID EHNMFEFWDF VGGRYSLWSA IGLSIMIYLG KKNFNALLKG AYLMDEHFRN APFESNLPVL MGLIGVWYIN FFQSKSHLIA PYDQYLRHFP KFIQQLDMES NGKRISKKGE TIPYDTCPVV WGDMGINAQH AFFQLLHQGT HLIPIDFIAS LDKKPNAKGH HEILFSNVLA QAQAFMKGKS YEEALGELLF KGLDKDEAKD LAHHRVFFGN RPSNILLLEK ISPSNIGALV ALYEHKVFVQ GVIWDINSFD QWGVELGKEL AVPILQELEG HKSNAYFDSS TKHLIELYKN YNQ
Validated NT Sequence
atggctcacc accaccacca ccatatgcta acccaattaa aaacttatcc aaaattactc aaacattatg aagaaattaa ggaagtgcac atgcacgatt ggttttctaa agacaaagag cgagcgagcc gttatttcgt gcaatttgaa agcttgagct tggattattc caaaaaccgc ttgaacgata ccactttaaa gcttcttttt gaattagcgg atgattgctc tttaaaagaa aagattgaag cgatgtttaa gggcgaaaaa atcaacacca ccgaaaaaag ggccgtttta cacaccgcct taagaagctt gaatgacgca gaaatcttac tagacaacat ggaagtgtta aaaagcgttc ggagcgtttt aaaacgcatg cgagccttta gcgatagcgt gaggagcggt aaaagactgg gctataccaa tcaagtgatc accgatattg tcaatatcgg cattgggggg tcagatttag gcgctttaat ggtttgcacc gccctaaaac gctacggcca cccgagatta aaaatgcatt ttgtgtctaa tgtggatggc acgcagattt tagatgtttt ggaaaaaatc aatccggcca gcacgctttt tatcgtggcc tctaagactt tttccactca agaaacctta accaacgccc taaccgctag aaaatggttt gtagaaagaa gcggcgatga aaagcatatc gctaagcact ttgtagcggt atccaccaac aaagaagccg tgcaacaatt tggcattgac gagcataaca tgtttgaatt ttgggatttt gtaggggggc gttatagctt gtggtcagct attggcttat ccattatgat ctatttaggg aagaaaaatt ttaacgctct tttgaagggg gcgtatttga tggatgagca ttttagaaac gccccttttg aaagcaattt acccgtttta atggggctaa ttggcgtgtg gtatatcaat tttttccaat ccaaaagcca cttgatcgct ccttatgatc agtatttgag gcatttccct aaattcattc agcaattgga tatggaaagt aatggcaaac gcatcagcaa aaaaggcgaa accatcccct atgacacatg ccctgttgtt tggggcgata tgggcattaa cgctcagcac gcctttttcc agctcttgca tcaaggtacg catttaatcc ccattgattt tatcgcttcc ttagataaaa agcctaacgc taaaggccac catgagattt tattcagcaa tgttttagcg caagcgcaag ccttcatgaa aggcaagagt tatgaagaag cgcttgggga attgctcttt aaaggcttag acaaagatga agccaaagac ttggcccacc acagggtgtt ttttggcaac cgcccctcta atatcctttt attagaaaag atttcaccaa gcaatattgg ggcgttggtg gctctttatg agcataaggt ctttgtgcaa ggggtcattt gggatattaa cagctttgat caatggggcg tggagcttgg gaaggaattg gccgtgccga ttttacaaga attagaaggg cataaaagca acgcttattt tgacagctcc actaagcatt taatagaatt gtataagaat tataaccaat aa
Expressed Protein Sequence
MAHHHHHHML TQLKTYPKLL KHYEEIKEVH MHDWFSKDKE RASRYFVQFE SLSLDYSKNR LNDTTLKLLF ELADDCSLKE KIEAMFKGEK INTTEKRAVL HTALRSLNDA EILLDNMEVL KSVRSVLKRM RAFSDSVRSG KRLGYTNQVI TDIVNIGIGG SDLGALMVCT ALKRYGHPRL KMHFVSNVDG TQILDVLEKI NPASTLFIVA SKTFSTQETL TNALTARKWF VERSGDEKHI AKHFVAVSTN KEAVQQFGID EHNMFEFWDF VGGRYSLWSA IGLSIMIYLG KKNFNALLKG AYLMDEHFRN APFESNLPVL MGLIGVWYIN FFQSKSHLIA PYDQYLRHFP KFIQQLDMES NGKRISKKGE TIPYDTCPVV WGDMGINAQH AFFQLLHQGT HLIPIDFIAS LDKKPNAKGH HEILFSNVLA QAQAFMKGKS YEEALGELLF KGLDKDEAKD LAHHRVFFGN RPSNILLLEK ISPSNIGALV ALYEHKVFVQ GVIWDINSFD QWGVELGKEL AVPILQELEG HKSNAYFDSS TKHLIELYKN YNQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTAA CCCAATTAAA AACTTATCCA AAATTACTCA AACATTATGA AGAAATTAAG GAAGTGCACA TGCACGATTG GTTTTCTAAA GACAAAGAGC GAGCGAGCCG TTATTTCGTG CAATTTGAAA GCTTGAGCTT GGATTATTCC AAAAACCGCT TGAACGATAC CACTTTAAAG CTTCTTTTTG AATTAGCGGA TGATTGCTCT TTAAAAGAAA AGATTGAAGC GATGTTTAAG GGCGAAAAAA TCAACACCAC CGAAAAAAGG GCCGTTTTAC ACACCGCCTT AAGAAGCTTG AATGACGCAG AAATCTTACT AGACAACATG GAAGTGTTAA AAAGCGTTCG GAGCGTTTTA AAACGCATGC GAGCCTTTAG CGATAGCGTG AGGAGCGGTA AAAGACTGGG CTATACCAAT CAAGTGATCA CCGATATTGT CAATATCGGC ATTGGGGGGT CAGATTTAGG CGCTTTAATG GTTTGCACCG CCCTAAAACG CTACGGCCAC CCGAGATTAA AAATGCATTT TGTGTCTAAT GTGGATGGCA CGCAGATTTT AGATGTTTTG GAAAAAATCA ATCCGGCCAG CACGCTTTTT ATCGTGGCCT CTAAGACTTT TTCCACTCAA GAAACCTTAA CCAACGCCCT AACCGCTAGA AAATGGTTTG TAGAAAGAAG CGGCGATGAA AAGCATATCG CTAAGCACTT TGTAGCGGTA TCCACCAACA AAGAAGCCGT GCAACAATTT GGCATTGACG AGCATAACAT GTTTGAATTT TGGGATTTTG TAGGGGGGCG TTATAGCTTG TGGTCAGCTA TTGGCTTATC CATTATGATC TATTTAGGGA AGAAAAATTT TAACGCTCTT TTGAAGGGGG CGTATTTGAT GGATGAGCAT TTTAGAAACG CCCCTTTTGA AAGCAATTTA CCCGTTTTAA TGGGGCTAAT TGGCGTGTGG TATATCAATT TTTTCCAATC CAAAAGCCAC TTGATCGCTC CTTATGATCA GTATTTGAGG CATTTCCCTA AATTCATTCA GCAATTGGAT ATGGAAAGTA ATGGCAAACG CATCAGCAAA AAAGGCGAAA CCATCCCCTA TGACACATGC CCTGTTGTTT GGGGCGATAT GGGCATTAAC GCTCAGCACG CCTTTTTCCA GCTCTTGCAT CAAGGTACGC ATTTAATCCC CATTGATTTT ATCGCTTCCT TAGATAAAAA GCCTAACGCT AAAGGCCACC ATGAGATTTT ATTCAGCAAT GTTTTAGCGC AAGCGCAAGC CTTCATGAAA GGCAAGAGTT ATGAAGAAGC GCTTGGGGAA TTGCTCTTTA AAGGCTTAGA CAAAGATGAA GCCAAAGACT TGGCCCACCA CAGGGTGTTT TTTGGCAACC GCCCCTCTAA TATCCTTTTA TTAGAAAAGA TTTCACCAAG CAATATTGGG GCGTTGGTGG CTCTTTATGA GCATAAGGTC TTTGTGCAAG GGGTCATTTG GGATATTAAC AGCTTTGATC AATGGGGCGT GGAGCTTGGG AAGGAATTGG CCGTGCCGAT TTTACAAGAA TTAGAAGGGC ATAAAAGCAA CGCTTATTTT GACAGCTCCA CTAAGCATTT AATAGAATTG TATAAGAATT ATAACCAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT