SSGCID: Target Page

HepyC.10647.a

Penicillin-binding protein 2

CENTER ID:	HepyC.10647.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.10647.a.B2.GE41015	mature protein		23	580

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1630
UniProt:	B5Z9J5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLFVFIGFWG LLVLNLFILS VKNQEYYKKL AERNMTKKEF LVPTRGDITD RNDEFLATNE LVFGVFLPSN LKQKELLEKI EIIQTFFPNF SKETLLNNYQ KENSLYNHNL IKVVGFIPYA AMQSLYAKLI QTQGIFVLPL DKRYYPNNAL ASHVLGYVGV ASLQDLKDDE ENQYSQIVGK TGIEKEYNKL LQGKVGYKIM RVNALNQELA TLEVVPPSTN NHLQLSLDER LQKEADKLFE NKRGAILVMN AENGELLVAG SYPEYNLNDF VGGISQDKWQ KLQDDIYNPL LNRFANALYP PGSVVKMGVG LSFLENLHIT ENTTIPTPPF IEVGKRKFRD WKKTGHGNSN LYKAIRESVD VYFYKFGLEI SIEKLSKTLR EVGFGEKTGV DLPNEFVGIV PDNLWKLKRF NQDWRVGDTL ITAIGQGSFL ATPLQVLAYT GLIATGKLAT PHFAINNKQP LKDPLNSFQK KKLQALRVGM YEVCNHKDGT AYHSTRGSKV TLACKTGTAQ VVEIAQNIVN RMKEKDMEYF HRSHAWITAF LPYEKPKYAI TILVEHGEGG SKLGGLLVKM SNKLYELGYL

NT Sequence

ttgctctttg tttttatagg gttttggggg ttattggttt taaatttatt tattttgagc gttaaaaatc aagaatacta taaaaaattg gccgaacgca acatgaccaa aaaggaattt ctagtcccta caaggggcga tattacagac agaaatgatg agtttttagc cactaacgaa ttggtgtttg gcgtgttttt gcccagcaac ctgaaacaaa aagaactttt ggaaaaaatt gaaatcatcc aaacattttt ccctaacttt tccaaagaaa cgcttttaaa caattaccaa aaagaaaatt cgctttataa ccataacctc attaaagtgg ttggcttcat tccttatgcc gccatgcaat ctctttatgc caaactcatc caaactcaag gcatttttgt gcttccctta gacaagcgct actaccctaa taacgcttta gcttcgcatg ttttaggcta tgtgggggtg gcaagcttgc aagatttaaa agacgatgag gagaatcaat acagccagat tgtgggcaaa accggcattg aaaaagaata caacaagctt ttgcaaggca aggtgggcta taaaatcatg cgtgtcaatg cactcaatca agaattagcc actttagaag tagtgccacc aagcaccaac aaccacttgc aattgagttt agacgaacgc ttgcaaaaag aagcggacaa gctctttgaa aataaaaggg gggctatttt agtgatgaac gccgaaaatg gggaattgct cgttgcaggg agttaccctg aatacaattt gaacgatttt gtaggcggga tcagtcaaga caaatggcaa aaacttcaag atgatattta taacccccta ctaaaccgct tcgctaacgc cttgtatccg ccgggatctg tggttaaaat gggcgtgggc ttgagctttt tagaaaacct tcatatcaca gaaaacacca ccatacccac cccgcctttt attgaagtgg gtaagcgcaa attcagagac tggaaaaaaa cagggcatgg caattctaat ttgtataaag ccattaggga gtctgtggat gtgtattttt ataagtttgg gcttgaaatt tctatagaaa aactctctaa aaccttaagg gaagtgggct ttggggaaaa aacgggcgtt gatttgccga atgaatttgt ggggattgtg ccggataatt tgtggaaact caaacgcttc aatcaagatt ggcgcgttgg ggacacgctc attaccgcta ttgggcaagg ctctttttta gccacgccct tacaagtgct agcctatacg ggactcattg cgacaggcaa actggcaacg cctcattttg ctatcaataa taaacaaccg ctcaaagacc ccctaaatag ttttcaaaaa aagaagctcc aagccttgag agtgggcatg tatgaagtgt gtaaccataa agacggcacc gcttatcatt ccacaagggg ttctaaggtt actttagcgt gtaaaaccgg caccgcgcaa gtcgtggaaa tcgctcaaaa catcgtcaat cgcatgaaag aaaaggatat ggaatatttc catcgatccc atgcgtggat tacggcattc ttgccttatg aaaaacccaa atacgctatc actattttag tagagcatgg ggaagggggg tcaaaactag ggggcttgtt agtgaaaatg agcaataaac tctatgagct tggctatctt taa

Details for HepyC.10647.a.B2.GE41015

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHNQ EYYKKLAERN MTKKEFLVPT RGDITDRNDE FLATNELVFG VFLPSNLKQK ELLEKIEIIQ TFFPNFSKET LLNNYQKENS LYNHNLIKVV GFIPYAAMQS LYAKLIQTQG IFVLPLDKRY YPNNALASHV LGYVGVASLQ DLKDDEENQY SQIVGKTGIE KEYNKLLQGK VGYKIMRVNA LNQELATLEV VPPSTNNHLQ LSLDERLQKE ADKLFENKRG AILVMNAENG ELLVAGSYPE YNLNDFVGGI SQDKWQKLQD DIYNPLLNRF ANALYPPGSV VKMGVGLSFL ENLHITENTT IPTPPFIEVG KRKFRDWKKT GHGNSNLYKA IRESVDVYFY KFGLEISIEK LSKTLREVGF GEKTGVDLPN EFVGIVPDNL WKLKRFNQDW RVGDTLITAI GQGSFLATPL QVLAYTGLIA TGKLATPHFA INNKQPLKDP LNSFQKKKLQ ALRVGMYEVC NHKDGTAYHS TRGSKVTLAC KTGTAQVVEI AQNIVNRMKE KDMEYFHRSH AWITAFLPYE KPKYAITILV EHGEGGSKLG GLLVKMSNKL YELGYL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatcaag aatactataa aaaattggcc gaacgcaaca tgaccaaaaa ggaatttcta gtccctacaa ggggcgatat tacagacaga aatgatgagt ttttagccac taacgaattg gtgtttggcg tgtttttgcc cagcaacctg aaacaaaaag aacttttgga aaaaattgaa atcatccaaa catttttccc taacttttcc aaagaaacgc ttttaaacaa ttaccaaaaa gaaaattcgc tttataacca taacctcatt aaagtggttg gcttcattcc ttatgccgcc atgcaatctc tttatgccaa actcatccaa actcaaggca tttttgtgct tcccttagac aagcgctact accctaataa cgctttagct tcgcatgttt taggctatgt gggggtggca agcttgcaag atttaaaaga cgatgaggag aatcaataca gccagattgt gggcaaaacc ggcattgaaa aagaatacaa caagcttttg caaggcaagg tgggctataa aatcatgcgt gtcaatgcac tcaatcaaga attagccact ttagaagtag tgccaccaag caccaacaac cacttgcaat tgagtttaga cgaacgcttg caaaaagaag cggacaagct ctttgaaaat aaaagggggg ctattttagt gatgaacgcc gaaaatgggg aattgctcgt tgcagggagt taccctgaat acaatttgaa cgattttgta ggcgggatca gtcaagacaa atggcaaaaa cttcaagatg atatttataa ccccctacta aaccgcttcg ctaacgcctt gtatccgccg ggatctgtgg ttaaaatggg cgtgggcttg agctttttag aaaaccttca tatcacagaa aacaccacca tacccacccc gccttttatt gaagtgggta agcgcaaatt cagagactgg aaaaaaacag ggcatggcaa ttctaatttg tataaagcca ttagggagtc tgtggatgtg tatttttata agtttgggct tgaaatttct atagaaaaac tctctaaaac cttaagggaa gtgggctttg gggaaaaaac gggcgttgat ttgccgaatg aatttgtggg gattgtgccg gataatttgt ggaaactcaa acgcttcaat caagattggc gcgttgggga cacgctcatt accgctattg ggcaaggctc ttttttagcc acgcccttac aagtgctagc ctatacggga ctcattgcga caggcaaact ggcaacgcct cattttgcta tcaataataa acaaccgctc aaagaccccc taaatagttt tcaaaaaaag aagctccaag ccttgagagt gggcatgtat gaagtgtgta accataaaga cggcaccgct tatcattcca caaggggttc taaggttact ttagcgtgta aaaccggcac cgcgcaagtc gtggaaatcg ctcaaaacat cgtcaatcgc atgaaagaaa aggatatgga atatttccat cgatcccatg cgtggattac ggcattcttg ccttatgaaa aacccaaata cgctatcact attttagtag agcatgggga aggggggtca aaactagggg gcttgttagt gaaaatgagc aataaactct atgagcttgg ctatctttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at