SSGCID: Target Page

HepyC.10201.a

Methionine--tRNA ligase (EC 6.1.1.10) (Methionyl-tRNA synthetase)

CENTER ID:	HepyC.10201.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.10201.a.B2.GE41013	soluble domain #1		1	444
HepyC.10201.a.B3.GE41014	soluble domain #2		465	650

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.10201.a.B3.PW38236	soluble domain #2		465	650

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1040
UniProt:	B5Z811

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQKSLITTPI YYVNDIPHIG HAYTTLIADT LKKYYTLQGE EVFFLTGTDE HGQKIEQSAR LRNQSPKAYA DSISAIFKDQ WDFFNLDYDG FIRTTDSEHQ KCVQNAFEIM FEKGDIYKGT YSGYYCVSCE SYCAISKTDN TNDKVLCPDC LRETALLEEE SYFFRLSAYE KPLLDFYAKN PEAILPVYRK NEVTSFIEQG LLDLSITRTS FEWGIPLPKK MNDPKHVVYV WLDALLNYAS ALGYLNDLDN KMAHFECARH IVGKDILRFH AIYWPAFLMS LNLPLFKQLC VHGWWTIEGV KMSKSLGNVL DAQKLAMEYG IEELRYFLLR EVPFGQDGDF SKKALVERIN ANLNNDLGNL LNRLLGMAKK YFNYSLKSAK VTAYYSKELE KAHQILDNAN SFVPKMQLHK ALEELFNIYD FLNKLIAKEE PWVLHKNNES EKLEALLSLI ANVLLQSSFL LYAFMPKSAM KLASAFRVEI TPNNYERFFK AKKLQDMILQ DTEPLFSKME KIEKTEKAGE VSPEKNEKEK KDTKEKAPLK QENYIGIEDF KKVEIKVGLI KEAQRIEKSN KLLRLKVDLG EGRLRQIISG IALDYEPESL VGQMVCVVAN LKPAKLMGEM SEGMILAVRD NDNLALISPT REKIAGSLIS

NT Sequence

atgcaaaaat cactgatcac aacccccatt tattatgtga atgacattcc tcatattggc catgcttata cgactttgat tgcggatact ttaaagaagt attacacgct tcaaggcgaa gaagtctttt ttttaaccgg caccgatgag catgggcaaa agatcgaaca aagcgcgagg cttaggaatc aaagccctaa agcttacgcc gatagcatta gtgcgatttt taaagaccag tgggattttt tcaatttgga ttatgatggt tttatccgca ccacagacag cgagcatcaa aaatgcgtgc agaacgcctt tgaaatcatg tttgaaaaag gggatattta taaaggcact tatagcgggt attattgcgt gagctgtgag agttattgcg cgatctctaa aacggacaat acgaacgata aagtcctatg ccctgattgc ttgagggaga ctgcgctttt agaagaagag agttattttt tcagattgag cgcgtatgag aagcctttat tggattttta cgctaaaaat cctgaagcga ttttgcccgt ttatcgtaaa aatgaggtaa cttcttttat tgagcagggt ttattggatc tgtctatcac gcgcacgagc tttgaatggg gcattccttt gcctaaaaaa atgaacgatc ctaaacatgt ggtgtatgtt tggctggacg ctttattgaa ttatgcgagt gcgttagggt atttgaacga tttagacaat aaaatggcgc attttgaatg cgctaggcat attgtgggta aggatatttt acgcttccat gccatttatt ggccggcttt tttgatgagt ttgaatttgc ccttattcaa acagctttgc gtgcatgggt ggtggacgat agagggcgtg aaaatgagta aaagcttggg taatgtttta gacgctcaaa agctcgctat ggagtatggg attgaagaat tacgctattt tttattgcgt gaggtgcctt ttgggcaaga tggggatttt tctaaaaaag cgttagtaga aaggatcaac gcgaatttga ataacgattt ggggaatttg ttgaatcgct tgctaggcat ggctaaaaaa tatttcaatt attctctaaa aagcgccaaa gtcaccgctt attattctaa agagctagaa aaagcgcatc aaatcttaga taacgctaat tcttttgtgc ctaaaatgca attgcataaa gctttagagg aattgtttaa tatttatgat tttttaaaca aactcatcgc taaagaagag ccatgggttt tgcacaaaaa caacgaatca gaaaaactag aagccttatt gagtttgatc gcaaacgtgc tgttgcaatc aagctttttg ctctatgcgt tcatgccaaa gagcgctatg aaattagcga gcgcttttcg tgtagaaatc acgcccaata attatgaacg cttttttaaa gctaaaaaat tacaagatat gattttacaa gacactgagc ctttattttc caaaatggag aaaattgaaa agactgaaaa agcgggagaa gtctcaccag aaaaaaacga aaaagaaaaa aaggacacaa aagaaaaagc cccactaaaa caagaaaact atatcggcat tgaggatttc aaaaaagtag agattaaggt ggggcttatc aaagaagctc aaaggattga aaaatccaat aaattattgc gcttgaaagt ggatttaggc gaaggccgtt tgaggcagat tatttcaggg atcgctttgg attatgagcc tgaaagcttg gtgggtcaaa tggtgtgcgt ggtggctaat ttaaaacccg caaagcttat gggtgaaatg agtgagggca tgattttagc ggtgcgagat aatgataatc tggctttaat tagccctacc agagaaaaaa ttgcaggaag tttgatcagc taa

Details for HepyC.10201.a.B2.GE41013

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	52kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMQ KSLITTPIYY VNDIPHIGHA YTTLIADTLK KYYTLQGEEV FFLTGTDEHG QKIEQSARLR NQSPKAYADS ISAIFKDQWD FFNLDYDGFI RTTDSEHQKC VQNAFEIMFE KGDIYKGTYS GYYCVSCESY CAISKTDNTN DKVLCPDCLR ETALLEEESY FFRLSAYEKP LLDFYAKNPE AILPVYRKNE VTSFIEQGLL DLSITRTSFE WGIPLPKKMN DPKHVVYVWL DALLNYASAL GYLNDLDNKM AHFECARHIV GKDILRFHAI YWPAFLMSLN LPLFKQLCVH GWWTIEGVKM SKSLGNVLDA QKLAMEYGIE ELRYFLLREV PFGQDGDFSK KALVERINAN LNNDLGNLLN RLLGMAKKYF NYSLKSAKVT AYYSKELEKA HQILDNANSF VPKMQLHKAL EELFNIYDFL NKLIAKEEPW VXTK

Validated NT Sequence

tttttgtnnn aacccatggc tcttctttag cgatgagttt gtttaaaaaa tcataaatat taaacaattc ctctaaagct ttatgcaatt gcattttagg cacaaaagaa ttagcgttat ctaagatttg atgcgctttt tctagctctt tagaataata agcggtgact ttggcgcttt ttagagaata attgaaatat tttttagcca tgcctagcaa gcgattcaac aaattcccca aatcgttatt caaattcgcg ttgatccttt ctactaacgc ttttttagaa aaatccccat cttgcccaaa aggcacctca cgcaataaaa aatagcgtaa ttcttcaatc ccatactcca tagcgagctt ttgagcgtct aaaacattac ccaagctttt actcattttc acgccctcta tcgtccacca cccatgcacg caaagctgtt tgaataaggg caaattcaaa ctcatcaaaa aagccggcca ataaatggca tggaagcgta aaatatcctt acccacaata tgcctagcgc attcaaaatg cgccatttta ttgtctaaat cgttcaaata ccctaacgca ctcgcataat tcaataaagc gtccagccaa acatacacca catgtttagg atcgttcatt tttttaggca aaggaatgcc ccattcaaag ctcgtgcgcg tgatagacag atccaataaa ccctgctcaa taaaagaagt tacctcattt ttacgataaa cgggcaaaat cgcttcagga tttttagcgt aaaaatccaa taaaggcttc tcatacgcgc tcaatctgaa aaaataactc tcttcttcta aaagcgcagt ctccctcaag caatcagggc ataggacttt atcgttcgta ttgtccgttt tagagatcgc gcaataactc tcacagctca cgcaataata cccgctataa gtgcctttat aaatatcccc tttttcaaac atgatttcaa aggcgttctg cacgcatttt tgatgctcgc tgtctgtggt gcggataaaa ccatcataat ccaaattgaa aaaatcccac tggtctttaa aaatcgcact aatgctatcg gcgtaagctt tagggctttg attcctaagc ctcgcgcttt gttcgatctt ttgcccatgc tcatcggtgc cggttaaaaa aaagacttct tcgccttgaa gcgtgtaata cttctttaaa gtatccgcaa tcaaagtcgt ataagcatgg ccaatatgag gaatgtcatt cacataataa atgggggttg tgatcagtga tttttgcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa aatcactgat cacaaccccc atttattatg tgaatgacat tcctcatatt ggccatgctt atacgacttt gattgcggat actttaaaga agtattacac gcttcaaggc gaagaagtct tttttttaac cggcaccgat gagcatgggc aaaagatcga acaaagcgcg aggcttagga atcaaagccc taaagcttac gccgatagca ttagtgcgat ttttaaagac cagtgggatt ttttcaattt ggattatgat ggttttatcc gcaccacaga cagcgagcat caaaaatgcg tgcagaacgc ctttgaaatc atgtttgaaa aaggggatat ttataaaggc acttatagcg ggtattattg cgtgagctgt gagagttatt gcgcgatctc taaaacggac aatacgaacg ataaagtcct atgccctgat tgcttgaggg agactgcgct tttagaagaa gagagttatt ttttcagatt gagcgcgtat gagaagcctt tattggattt ttacgctaaa aatcctgaag cgattttgcc cgtttatcgt aaaaatgagg taacttcttt tattgagcag ggtttattgg atctgtctat cacgcgcacg agctttgaat ggggcattcc tttgcctaaa aaaatgaacg atcctaaaca tgtggtgtat gtttggctgg acgctttatt gaattatgcg agtgcgttag ggtatttgaa cgatttagac aataaaatgg cgcattttga atgcgctagg catattgtgg gtaaggatat tttacgcttc catgccattt attggccggc ttttttgatg agtttgaatt tgcccttatt caaacagctt tgcgtgcatg ggtggtggac gatagagggc gtgaaaatga gtaaaagctt gggtaatgtt ttagacgctc aaaagctcgc tatggagtat gggattgaag aattacgcta ttttttattg cgtgaggtgc cttttgggca agatggggat ttttctaaaa aagcgttagt agaaaggatc aacgcgaatt tgaataacga tttggggaat ttgttgaatc gcttgctagg catggctaaa aaatatttca attattctct aaaaagcgcc aaagtcaccg cttattattc taaagagcta gaaaaagcgc atcaaatctt agataacgct aattcttttg tgcctaaaat gcaattgcat aaagctttag aggaattgtt taatatttat gattttttaa acaaactcat cgctaaagaa gagccatggg ttttgcacaa aaacaacgaa tcagaaaaac tagaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for HepyC.10201.a.B3.GE41014

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP KSAMKLASAF RVEITPNNYE RFFKAKKLQD MILQDTEPLF SKMEKIEKTE KAGEVSPEKN EKEKKDTKEK APLKQENYIG IEDFKKVEIK VGLIKEAQRI EKSNKLLRLK VDLGEGRLRQ IISGIALDYE PESLVGQMVC VVANLKPAKL MGEMSEGMIL AVRDNDNLAL ISPTREKIAG SLIS

Validated NT Sequence

atcttactca cttagctgat caaacttcct gcaatttttt ctctggtagg gctaattaaa gccagattat cattatctcg caccgctaaa atcatgccct cactcatttc acccataagc tttgcgggtt ttaaattagc caccacgcac accatttgac ccaccaagct ttcaggctca taatccaaag cgatccctga aataatctgc ctcaaacggc cttcgcctaa atccactttc aagcgcaata atttattgga tttttcaatc ctttgagctt ctttgataag ccccacctta atctctactt ttttgaaatc ctcaatgccg atatagtttt cttgttttag tggggctttt tcttttgtgt cctttttttc tttttcgttt ttttctggtg agacttctcc cgctttttca gtcttttcaa ttttctccat tttggaaaat aaaggctcag tgtcttgtaa aatcatatct tgtaattttt tagctttaaa aaagcgttca taattattgg gcgtgatttc tacacgaaaa gcgctcgcta atttcatagc gctctttggc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa agagcgctat gaaattagcg agcgcttttc gtgtagaaat cacgcccaat aattatgaac gcttttttaa agctaaaaaa ttacaagata tgattttaca agacactgag cctttatttt ccaaaatgga gaaaattgaa aagactgaaa aagcgggaga agtctcacca gaaaaaaacg aaaaagaaaa aaaggacaca aaagaaaaag ccccactaaa acaagaaaac tatatcggca ttgaggattt caaaaaagta gagattaagg tggggcttat caaagaagct caaaggattg aaaaatccaa taaattattg cgcttgaaag tggatttagg cgaaggccgt ttgaggcaga ttatttcagg gatcgctttg gattatgagc ctgaaagctt ggtgggtcaa atggtgtgcg tggtggctaa tttaaaaccc gcaaagctta tgggtgaaat gagtgagggc atgattttag cggtgcgaga taatgataat ctggctttaa ttagccctac cagagaaaaa attgcaggaa gtttgatcag ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for HepyC.10201.a.B3.PW38236

PURIFICATION DATe:	3/27/2017
CONCENTRATION:	3.3mg/ml
OBSERVED MW:	23kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 8.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	27
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAA AGAGCGCTAT GAAATTAGCG AGCGCTTTTC GTGTAGAAAT CACGCCCAAT AATTATGAAC GCTTTTTTAA AGCTAAAAAA TTACAAGATA TGATTTTACA AGACACTGAG CCTTTATTTT CCAAAATGGA GAAAATTGAA AAGACTGAAA AAGCGGGAGA AGTCTCACCA GAAAAAAACG AAAAAGAAAA AAAGGACACA AAAGAAAAAG CCCCACTAAA ACAAGAAAAC TATATCGGCA TTGAGGATTT CAAAAAAGTA GAGATTAAGG TGGGGCTTAT CAAAGAAGCT CAAAGGATTG AAAAATCCAA TAAATTATTG CGCTTGAAAG TGGATTTAGG CGAAGGCCGT TTGAGGCAGA TTATTTCAGG GATCGCTTTG GATTATGAGC CTGAAAGCTT GGTGGGTCAA ATGGTGTGCG TGGTGGCTAA TTTAAAACCC GCAAAGCTTA TGGGTGAAAT GAGTGAGGGC ATGATTTTAG CGGTGCGAGA TAATGATAAT CTGGCTTTAA TTAGCCCTAC CAGAGAAAAA ATTGCAGGAA GTTTGATCAG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT