SSGCID: Target Page

HepyC.10014.b

Glutamine ABC transporter, ATP-binding protein

CENTER ID:	HepyC.10014.b
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.10014.b.B1.GE41012	full length		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1214
UniProt:	B5Z8G6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVILETKGL KKTYQNHLVL DGINFTLNKG EVAVILGPSG CGKSTFLKCL NGLEKIDEGE ILFENTNLNT NATNWNQMRQ KIGMVFQNYE LFPHLNVLDN ILLAPMKVQK RSKDEVISQA VELLKRVGLE HKQQAYPKEL SGGQKQRVAI VRSLCMRPKI MLFDEVTASL DPEMVKEVLE VILELATTGM SMVIVTHEMK FAQKIAHKIV FFDSGKIAEE NSAKEFFNHP KSQRAQKFLE TFHFLGSC

NT Sequence

atgagcgtga ttttagaaac caaagggtta aaaaaaacct atcaaaacca tttggtttta gacggcatca atttcacttt aaataagggt gaagtggcag tgattttagg gcctagcggg tgcgggaaaa gcactttttt aaaatgccta aacgggcttg aaaagattga tgaaggtgaa attctttttg aaaacactaa cctcaacacc aacgccacta actggaatca aatgcgccaa aaaataggca tggtgtttca aaattatgaa ttgttcccgc atttaaacgt gctggataat atcttactcg ctcctatgaa agtgcaaaaa cgatccaaag atgaggttat ttctcaagcc gtagagcttt taaagcgagt gggtttggag cataaacaac aagcttaccc taaagaattg agcggtgggc aaaaacaacg agtagcgatc gtgcgttctt tgtgcatgcg gccaaaaatc atgctttttg atgaagtaac cgcctcatta gaccctgaaa tggttaaaga agttttagaa gtgattttag aactagccac aacgggcatg agcatggtga ttgtaacgca tgaaatgaaa ttcgcgcaaa aaatcgctca caaaatcgtg ttttttgata gcggtaaaat cgctgaagaa aacagcgcta aagaattttt taaccacccg aaatctcaaa gagcgcaaaa atttttagaa actttccatt ttttagggag ctgttaa

Details for HepyC.10014.b.B1.GE41012

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	29kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS VILETKGLKK TYQNHLVLDG INFTLNKGEV AVILGPSGCG KSTFLKCLNG LEKIDEGEIL FENTNLNTNA TNWNQMRQKI GMVFQNYELF PHLNVLDNIL LAPMKVQKRS KDEVISQAVE LLKRVGLEHK QQAYPKELSG GQKQRVAIVR SLCMRPKIML FDEVTASLDP EMVKEVLEVI LELATTGMSM VIVTHEMKFA QKIAHKIVFF DSGKIAEENS AKEFFNHPKS QRAQKFLEXX XFLGSC

Validated NT Sequence

atcttactca cttaacagct ccctaaaaan nnnannnttt ctaaaaattt ttgcgctctt tgagatttcg ggtggttaaa aaattcttta gcgctgtttt cttcagcgat tttaccgcta tcaaaaaaca cgattttgtg agcgattttt tgcgcgaatt tcatttcatg cgttacaatc accatgctca tgcccgttgt ggctagttct aaaatcactt ctaaaacttc tttaaccatt tcagggtcta atgaggcggt tacttcatca aaaagcatga tttttggccg catgcacaaa gaacgcacga tcgctactcg ttgtttttgc ccaccgctca attctttagg gtaagcttgt tgtttatgct ccaaacccac tcgctttaaa agctctacgg cttgagaaat aacctcatct ttggatcgtt tttgcacttt cataggagcg agtaagatat tatccagcac gtttaaatgc gggaacaatt cataattttg aaacaccatg cctatttttt ggcgcatttg attccagtta gtggcgttgg tgttgaggtt agtgttttca aaaagaattt caccttcatc aatcttttca agcccgttta ggcattttaa aaaagtgctt ttcccgcacc cgctaggccc taaaatcact gccacttcac ccttatttaa agtgaaattg atgccgtcta aaaccaaatg gttttgatag gtttttttta accctttggt ttctaaaatc acgctcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tgattttaga aaccaaaggg ttaaaaaaaa cctatcaaaa ccatttggtt ttagacggca tcaatttcac tttaaataag ggtgaagtgg cagtgatttt agggcctagc gggtgcggga aaagcacttt tttaaaatgc ctaaacgggc ttgaaaagat tgatgaaggt gaaattcttt ttgaaaacac taacctcaac accaacgcca ctaactggaa tcaaatgcgc caaaaaatag gcatggtgtt tcaaaattat gaattgttcc cgcatttaaa cgtgctggat aatatcttac tcgctcctat gaaagtgcaa aaacgatcca aagatgaggt tatttctcaa gccgtagagc ttttaaagcg agtgggtttg gagcataaac aacaagctta ccctaaagaa ttgagcggtg ggcaaaaaca acgagtagcg atcgtgcgtt ctttgtgcat gcggccaaaa atcatgcttt ttgatgaagt aaccgcctca ttagaccctg aaatggttaa agaagtttta gaagtgattt tagaactagc cacaacgggc atgagcatgg tgattgtaac gcatgaaatg aaattcgcgc aaaaaatcgc tcacaaaatc gtgttttttg atagcggtaa aatcgctgaa gaaaacagcg ctaaagaatt ttttaaccac ccgaaatctc aaagagcgca aaaattttta gaaactttcc attttttagg gagctgttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at