SSGCID: Target Page

HepyC.01348.a

Glutamate--tRNA ligase 2 (EC 6.1.1.17) (Glutamyl-tRNA synthetase 2) (GluRS 2)

CENTER ID:	HepyC.01348.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.01348.a.B1.GE40963	full length		1	439

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.649
UniProt:	B5Z716

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRFAPSPTG DMHIGNLRAA IFNYIVAKQQ HKPFLIRIED TDKERNIEGK DQEILEILKL MGISWDKLVY QSHNIDYHRE MAEKLLKENK AFYCYASAEF LEREKEKAKN EKRPFRYLDE WATLEKDKNH APVVRLKAPN HAVSFNDAIK KEVEFEPDEL DSFVLLRQDK SPTYNFACAC DDLLYEISLI IRGEDHVSNT PKQILIQEAL GSNDPIVYAH LPIILDEVSG KKMSKRDEAS SVKWLLNQGF LPVAIANYLI TIGNKVPKEV FSLDEAIEWF SLENLSNSPA HFNLKYLKHL NHEHLKLLDD EKLLKLTSIK DKNLLGLLRL FIEECGTLLE LKEKISLFLE PKDIVKTYEN EDFKERCLVL FNALKSMDFQ AYKDFESFKK EAMRLSQLKG KDFFKPLRIL LTGNSHGVEL PLIFPYIQSH YQEILRLKA

NT Sequence

atgcttcgtt ttgcgccttc gcctacaggg gatatgcaca tagggaattt aagggcagcc attttcaact acattgtagc caaacagcaa cacaaaccct ttctcattcg cattgaagac acggacaaag agcgcaacat tgaaggcaaa gaccaggaaa ttttagagat tttaaagctt atggggataa gctgggacaa gctcgtgtat caaagccata acatagatta ccacagagaa atggcggaaa aattactgaa agaaaataaa gcgttttatt gttatgcgag cgcggagttt ttagaaagag aaaaagaaaa agccaaaaac gagaaacgcc cttttaggta tttagacgag tgggccactt tagaaaaaga caaaaaccat gcccctgtgg tgcgtttaaa agctcccaat catgcggtgt ctttcaatga tgcgattaaa aaagaagtgg aatttgagcc tgatgaattg gattcttttg tgcttttgag acaggataaa agccctactt ataatttcgc ttgcgcatgc gatgatttgc tctatgaaat cagcctgatt attagaggtg aagatcatgt gagtaacacc cccaaacaaa tcttaatcca agaagcttta ggctcaaacg atccgattgt ttatgcgcat ttgcccatta ttttagatga agtaagcggt aaaaagatga gcaaaagaga cgaagcgtcc agcgtgaaat ggcttttgaa tcaagggttt ttaccggttg cgattgcgaa ttacctcatc actatcggca ataaagtgcc taaggaagtt tttagccttg atgaagcgat agaatggttt agtttagaaa atctttctaa ttccccggct cattttaatt taaaatattt aaaacactta aaccacgagc atttaaagct tttagacgat gaaaagctat taaaactcac ttcaataaaa gataaaaatc ttttagggct tttaagattg tttatagaag aatgcggcac gcttttagaa ttgaaagaaa aaatttcgtt gtttttagag ccaaaagata ttgttaaaac ttatgaaaat gaagatttta aagagcgttg tttagtgctt tttaacgctc taaaaagcat ggattttcaa gcctataagg attttgaaag ttttaaaaaa gaagccatgc gattaagcca gcttaagggt aaggattttt tcaaaccttt acgcattctt ttaaccggga actcgcatgg cgttgaattg cctttgattt tcccctatat ccaaagccat tatcaagaaa ttttaaggct caaagcatga

Details for HepyC.01348.a.B1.GE40963

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/27/2016
EXPECTED MW:	52kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML RFAPSPTGDM HIGNLRAAIF NYIVAKQQHK PFLIRIEDTD KERNIEGKDQ EILEILKLMG ISWDKLVYQS HNIDYHREMA EKLLKENKAF YCYASAEFLE REKEKAKNEK RPFRYLDEWA TLEKDKNHAP VVRLKAPNHA VSFNDAIKKE VEFEPDELDS FVLLRQDKSP TYNFACACDD LLYEISLIIR GEDHVSNTPK QILIQEALGS NDPIVYAHLP IILDEVSGKK MSKRDEASSV KWLLNQGFLP VAIANYLITI GNKVPKEVFS LDEAIEWFSL ENLSNSPAHF NLKYLKHLNH EHLKLLDDEK LLKLTSIKDK NLLGLLRLFI EECGTLLELK EKISLFLEPK DIVKTYENED FKERCLVLFN ALKSMDFQAY KDFESFKKEA MRLSQLKGKD FFKPLRILLT GNSHGVELPL IFPYIQSHYQ

Validated NT Sequence

cttgataatg gctttggata taggggaaaa tcaaaggcaa ttcaacgcca tgcgagttcc cggttaaaag aatgcgtaaa ggtttgaaaa aatccttacc cttaagctgg cttaatcgca tggcttcttt tttaaaactt tcaaaatcct tataggcttg aaaatccatg ctttttagag cgttaaaaag cactaaacaa cgctctttaa aatcttcatt ttcataagtt ttaacaatat cttttggctc taaaaacaac gaaatttttt ctttcaattc taaaagcgtg ccgcattctt ctataaacaa tcttaaaagc cctaaaagat ttttatcttt tattgaagtg agttttaata gcttttcatc gtctaaaagc tttaaatgct cgtggtttaa gtgttttaaa tattttaaat taaaatgagc cggggaatta gaaagatttt ctaaactaaa ccattctatc gcttcatcaa ggctaaaaac ttccttaggc actttattgc cgatagtgat gaggtaattc gcaatcgcaa ccggtaaaaa cccttgattc aaaagccatt tcacgctgga cgcttcgtct cttttgctca tctttttacc gcttacttca tctaaaataa tgggcaaatg cgcataaaca atcggatcgt ttgagcctaa agcttcttgg attaagattt gtttgggggt gttactcaca tgatcttcac ctctaataat caggctgatt tcatagagca aatcatcgca tgcgcaagcg aaattataag tagggctttt atcctgtctc aaaagcacaa aagaatccaa ttcatcaggc tcaaattcca cttctttttt aatcgcatca ttgaaagaca ccgcatgatt gggagctttt aaacgcacca caggggcatg gtttttgtct ttttctaaag tggcccactc gtctaaatac ctaaaagggc gtttctcgtt tttggctttt tctttttctc tttctaaaaa ctccgcgctc gcataacaat aaaacgcttt attttctttc agtaattttt ccgccatttc tctgtggtaa tctatgttat ggctttgata cacgagcttg tcccagctta tccccataag ctttaaaatc tctaaaattt cctggtcttt gccttcaatg ttgcgctctt tgtccgtgtc ttcaatgcga atgagaaagg gtttgtgttg ctgtttggct acaatgtagt tgaaaatggc tgcccttaaa ttccctatgt gcatatcccc tgtaggcgaa ggcgcaaaac gaagcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttc gttttgcgcc ttcgcctaca ggggatatgc acatagggaa tttaagggca gccattttca actacattgt agccaaacag caacacaaac cctttctcat tcgcattgaa gacacggaca aagagcgcaa cattgaaggc aaagaccagg aaattttaga gattttaaag cttatgggga taagctggga caagctcgtg tatcaaagcc ataacataga ttaccacaga gaaatggcgg aaaaattact gaaagaaaat aaagcgtttt attgttatgc gagcgcggag tttttagaaa gagaaaaaga aaaagccaaa aacgagaaac gcccttttag gtatttagac gagtgggcca ctttagaaaa agacaaaaac catgcccctg tggtgcgttt aaaagctccc aatcatgcgg tgtctttcaa tgatgcgatt aaaaaagaag tggaatttga gcctgatgaa ttggattctt ttgtgctttt gagacaggat aaaagcccta cttataattt cgcttgcgca tgcgatgatt tgctctatga aatcagcctg attattagag gtgaagatca tgtgagtaac acccccaaac aaatcttaat ccaagaagct ttaggctcaa acgatccgat tgtttatgcg catttgccca ttattttaga tgaagtaagc ggtaaaaaga tgagcaaaag agacgaagcg tccagcgtga aatggctttt gaatcaaggg tttttaccgg ttgcgattgc gaattacctc atcactatcg gcaataaagt gcctaaggaa gtttttagcc ttgatgaagc gatagaatgg tttagtttag aaaatctttc taattccccg gctcatttta atttaaaata tttaaaacac ttaaaccacg agcatttaaa gcttttagac gatgaaaagc tattaaaact cacttcaata aaagataaaa atcttttagg gcttttaaga ttgtttatag aagaatgcgg cacgctttta gaattgaaag aaaaaatttc gttgttttta gagccaaaag atattgttaa aacttatgaa aatgaagatt ttaaagagcg ttgtttagtg ctttttaacg ctctaaaaag catggatttt caagcctata aggattttga aagttttaaa aaagaagcca tgcgattaag ccagcttaag ggtaaggatt ttttcaaacc tttacgcatt cttttaaccg ggaactcgca tggcgttgaa ttgcctttga ttttccccta tatccaaagc cattatcaag aaattttaag gctcaaagca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat