HepyC.01328.a

3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38) (CMP-2-keto-3-deoxyoctulosonic acid synthase) (CKS) (CMP-KDO synthase)

CENTER ID: HepyC.01328.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.01328.a.B1.GE40961 full length 1 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.236
UniProt: B5Z9Z7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIIIPARLKS SRFENKVLED IFGLPMVVRC AKNANLVDEC VVACDDESIM QTCQKFHIKA VLTSKHHNSG TERCLEAVQI LGLKNDERVL NLQGDEPFLE KEVILALLEA TKNAPFMATC AKVIDEEQAK SPNLVKVVLD SQNNALYFSR SLIPFLRDFD AKRQTPLLGH IGIYGFHNKE ILEELCALKP CVLEEIEKLE QLRALYYQKK ILVKIVQSQS VGIDTQEDLQ NALKIFSPDL LER
NT Sequence
atgattatca ttcctgctag gttaaaatcc agtcgttttg aaaataaagt gctagaagat atttttggct tgcctatggt agtgcgttgt gctaaaaatg caaatcttgt agatgaatgc gtagtcgctt gcgatgatga aagcatcatg caaacatgcc aaaaattcca cattaaagcg gtgctaacct ccaaacacca taatagcggc acggaacgct gtttggaagc ggtgcaaatt ttagggttaa aaaacgatga aagggtttta aacctgcaag gcgatgagcc ttttttagaa aaagaagtca ttttagcctt attagaagcc actaaaaacg cccctttcat ggcgacttgc gctaaagtca ttgatgaaga gcaggccaaa agccccaatt tagtcaaggt ggttttagac agccaaaata acgccttgta tttttcgcgc tcccttatcc cttttttacg agattttgat gcgaaacgcc aaacccccct tttagggcat atcggtattt atggcttcca caataaagaa atattagaag aattatgcgc tttaaaacct tgcgttttag aggaaataga aaaattagag caattaaggg ctttgtatta ccaaaaaaag attctagtaa aaatcgttca aagccaaagc gtgggcattg acacccaaga agatttgcaa aacgctttga aaatttttag ccccgatctc cttgagcgat aa
Details for HepyC.01328.a.B1.GE40961
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM IIIPARLKSS RFENKVLEDI FGLPMVVRCA KNANLVDECV VACDDESIMQ TCQKFHIKAV LTSKHHNSGT ERCLEAVQIL GLKNDERVLN LQGDEPFLEK EVILALLEAT KNAPFMATCA KVIDEEQAKS PNLVKVVLDS QNNALYFSRS LIPFLRDFDA KRQTPLLGHI GIYGFHNKEI LEELCALKPC VLEEIEKLEQ LRALYYQKKI LVKIVQSQSV GIDTQEDLQN ALKIXXPDLL ER
Validated NT Sequence
atcttactca cttatcgctc aaggagatcg gggnnnnnaa ttttcaaagc gttttgcaaa tcttcttggg tgtcaatgcc cacgctttgg ctttgaacga tttttactag aatctttttt tggtaataca aagcccttaa ttgctctaat ttttctattt cctctaaaac gcaaggtttt aaagcgcata attcttctaa tatttcttta ttgtggaagc cataaatacc gatatgccct aaaagggggg tttggcgttt cgcatcaaaa tctcgtaaaa aagggataag ggagcgcgaa aaatacaagg cgttattttg gctgtctaaa accaccttga ctaaattggg gcttttggcc tgctcttcat caatgacttt agcgcaagtc gccatgaaag gggcgttttt agtggcttct aataaggcta aaatgacttc tttttctaaa aaaggctcat cgccttgcag gtttaaaacc ctttcatcgt tttttaaccc taaaatttgc accgcttcca aacagcgttc cgtgccgcta ttatggtgtt tggaggttag caccgcttta atgtggaatt tttggcatgt ttgcatgatg ctttcatcat cgcaagcgac tacgcattca tctacaagat ttgcattttt agcacaacgc actaccatag gcaagccaaa aatatcttct agcactttat tttcaaaacg actggatttt aacctagcag gaatgataat catatggtgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMI IIPARLKSSR FENKVLEDIF GLPMVVRCAK NANLVDECVV ACDDESIMQT CQKFHIKAVL TSKHHNSGTE RCLEAVQILG LKNDERVLNL QGDEPFLEKE VILALLEATK NAPFMATCAK VIDEEQAKSP NLVKVVLDSQ NNALYFSRSL IPFLRDFDAK RQTPLLGHIG IYGFHNKEIL EELCALKPCV LEEIEKLEQL RALYYQKKIL VKIVQSQSVG IDTQEDLQNA LKIFSPDLLE R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta tcattcctgc taggttaaaa tccagtcgtt ttgaaaataa agtgctagaa gatatttttg gcttgcctat ggtagtgcgt tgtgctaaaa atgcaaatct tgtagatgaa tgcgtagtcg cttgcgatga tgaaagcatc atgcaaacat gccaaaaatt ccacattaaa gcggtgctaa cctccaaaca ccataatagc ggcacggaac gctgtttgga agcggtgcaa attttagggt taaaaaacga tgaaagggtt ttaaacctgc aaggcgatga gcctttttta gaaaaagaag tcattttagc cttattagaa gccactaaaa acgccccttt catggcgact tgcgctaaag tcattgatga agagcaggcc aaaagcccca atttagtcaa ggtggtttta gacagccaaa ataacgcctt gtatttttcg cgctccctta tccctttttt acgagatttt gatgcgaaac gccaaacccc ccttttaggg catatcggta tttatggctt ccacaataaa gaaatattag aagaattatg cgctttaaaa ccttgcgttt tagaggaaat agaaaaatta gagcaattaa gggctttgta ttaccaaaaa aagattctag taaaaatcgt tcaaagccaa agcgtgggca ttgacaccca agaagatttg caaaacgctt tgaaaatttt tagccccgat ctccttgagc gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat