SSGCID: Target Page

HepyC.01294.a

Transketolase (EC 2.2.1.1)

CENTER ID:	HepyC.01294.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.01294.a.B2.GE40959	soluble domain #1		1	406
HepyC.01294.a.B3.GE40960	soluble domain #2		429	641

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.376
UniProt:	B5ZAC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLSNADLER LKSMANALRF LCADMIDKAN SGHPGVCLGL ADVMVVLSLH LNLNPTNPKW LNRDRLVFSG GHASALAYSL LHLWGFDLSL EDLKRFRQLH SKTPGHPELH HTEGIEITTG PLGQGFANAV GFSMASQYAQ NLLDKEAISH KVYCLCGDGD LQEGISYESA SLAGHLRLDN LIVIYDSNQI SIEGTINISF SEQVKTRFLA QNWEVLECDG HDYQAIHDAL EEAKKSHKPT LLIAHTIIGK GAIGLEGSEK THGSPLNKEV LKQSKENAQI NPDESFIISP KNKMHFEEVK VRGVSLEALW EKSLSPKIKE KIHALKNFDF NTIHYPTFKK GESLATRVSN GMILNAIAKE CEGFLGGSAD LAPSNNTHLK HSGDFPLGQN LHFGIREHAM GAITNALAAY GLFVPFCATF FVFSDYLMPS MRLSALMKLK ALFIFTHDSI GVGEDGATHQ PIEQLSHLRA LPNFYAFRPS DAFENTACMQ IALSLNAPSA LILSRQNLPV LDEVSKEQVL KGAYVKYDAK DPIITLVASG SEVSLALESA KILERENIQT QVISAPCFDL LIEQDESYLK ELFKGKVLVI EASRAIEWYR FADKIIGMDS FGSSAKGDKL FEKFGFSVEN ITAQAKRLLN A

NT Sequence

atgcgattga gtaacgctga cttagaacga ttaaaaagca tggcgaatgc gcttcgcttt ttgtgcgcgg acatgataga taaggctaat agcgggcatc cgggcgtgtg cttggggtta gctgatgtga tggtggtttt aagcttgcac ctaaacctaa accccactaa ccctaaatgg ctcaataggg acaggttggt ttttagcgga gggcatgcga gcgcattagc gtatagtttg ttgcatttgt ggggctttga tttgagctta gaagatttaa agcgtttcag gcaattacac tctaaaaccc caggacaccc cgaattgcac cacaccgaag gcattgaaat caccacaggc cctttagggc aaggttttgc taacgctgtg ggctttagca tggcgagcca atacgctcaa aaccttttgg ataaagaagc gatttcccat aaagtctatt gcttgtgtgg ggatggggat ttgcaagaag gcattagtta tgagagcgct tctttggccg ggcaccttcg ccttgataat ctcattgtga tttatgacag caaccagatc agcattgaag gcactatcaa tattagtttt agcgaacagg ttaaaactcg ttttttagcg caaaattggg aagttctaga atgcgatggg catgattatc aagcgattca tgatgcttta gaagaagcca aaaaatccca taaacccacg cttttaatcg ctcatacgat cattggtaag ggggctattg gtttagaggg aagtgaaaaa acgcatggct cgcctttaaa taaagaagtg ttaaaacaat ccaaagaaaa cgctcaaatc aatcctgatg aaagctttat cattagccca aaaaacaaaa tgcattttga agaagtgaaa gttaggggcg ttagcttaga agccttatgg gagaaatcct taagccctaa aataaaagaa aaaatccatg cgttaaagaa ttttgatttt aacaccatcc attaccccac ctttaaaaaa ggcgaatctc tagccacgag agtgagtaac ggcatgattt taaacgctat cgccaaagaa tgcgagggct ttttaggggg aagcgcggat ttagccccct ctaataacac gcatttaaaa cactctggcg atttcccttt agggcaaaac ttgcattttg ggatcagaga gcatgccatg ggggctatca ctaacgcttt agcggcgtat ggcttgtttg tgcctttttg cgcgaccttt tttgtgttta gcgattattt aatgcccagc atgcgtttga gcgctttaat gaaattaaaa gcccttttta tcttcacgca tgacagcatt ggcgtgggcg aagacggggc gacgcaccaa cccatagagc aattgagcca tttacgcgct ttgcctaatt tttatgcttt cagacccagc gacgcttttg aaaatacggc ttgcatgcaa atagcgttaa gtttgaacgc tcctagcgct cttattttat cgcgccagaa tttgcccgtg cttgatgagg tttctaaaga gcaggtttta aaaggcgcgt atgttaaata tgatgctaaa gatcccatta tcacgctcgt tgcgagcggg agcgaagtct ctttggcttt agagagcgct aaaattttag agcgagaaaa tatccaaacg caagtcatca gcgcgccatg ctttgattta ttgatagagc aagatgaaag ctatcttaaa gaactcttta agggtaaggt tttagtgatt gaagcgagcc gtgcgataga gtggtatcgt tttgcggata aaatcattgg catggattct tttgggagct cagcaaaggg cgataaactc tttgaaaaat ttggctttag cgttgaaaac attaccgctc aagcgaaaag attactcaac gcatga

Details for HepyC.01294.a.B2.GE40959

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	46kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR LSNADLERLK SMANALRFLC ADMIDKANSG HPGVCLGLAD VMVVLSLHLN LNPTNPKWLN RDRLVFSGGH ASALAYSLLH LWGFDLSLED LKRFRQLHSK TPGHPELHHT EGIEITTGPL GQGFANAVGF SMASQYAQNL LDKEAISHKV YCLCGDGDLQ EGISYESASL AGHLRLDNLI VIYDSNQISI EGTINISFSE QVKTRFLAQN WEVLECDGHD YQAIHDALEE AKKSHKPTLL IAHTIIGKGA IGLEGSEKTH GSPLNKEVLK QSKENAQINP DESFIISPKN KMHFEEVKVR GVSLEALWEK SLSPKIKEKI HALKNFDFNT IHYPTFKKGE SLATRVSNGM ILNAIAKECE GFLGGSADLA PSNNTHLKHS GDFPLGQNLH FGIREHA

Validated NT Sequence

tggcatgctc tctgatccca aaatgcaagt tttgccctaa agggaaatcg ccagagtgtt ttaaatgcgt gttattagag ggggctaaat ccgcgcttcc ccctaaaaag ccctcgcatt ctttggcgat agcgtttaaa atcatgccgt tactcactct cgtggctaga gattcgcctt ttttaaaggt ggggtaatgg atggtgttaa aatcaaaatt ctttaacgca tggatttttt cttttatttt agggcttaag gatttctccc ataaggcttc taagctaacg cccctaactt tcacttcttc aaaatgcatt ttgttttttg ggctaatgat aaagctttca tcaggattga tttgagcgtt ttctttggat tgttttaaca cttctttatt taaaggcgag ccatgcgttt tttcacttcc ctctaaacca atagccccct taccaatgat cgtatgagcg attaaaagcg tgggtttatg ggattttttg gcttcttcta aagcatcatg aatcgcttga taatcatgcc catcgcattc tagaacttcc caattttgcg ctaaaaaacg agttttaacc tgttcgctaa aactaatatt gatagtgcct tcaatgctga tctggttgct gtcataaatc acaatgagat tatcaaggcg aaggtgcccg gccaaagaag cgctctcata actaatgcct tcttgcaaat ccccatcccc acacaagcaa tagactttat gggaaatcgc ttctttatcc aaaaggtttt gagcgtattg gctcgccatg ctaaagccca cagcgttagc aaaaccttgc cctaaagggc ctgtggtgat ttcaatgcct tcggtgtggt gcaattcggg gtgtcctggg gttttagagt gtaattgcct gaaacgcttt aaatcttcta agctcaaatc aaagccccac aaatgcaaca aactatacgc taatgcgctc gcatgccctc cgctaaaaac caacctgtcc ctattgagcc atttagggtt agtggggttt aggtttaggt gcaagcttaa aaccaccatc acatcagcta accccaagca cacgcccgga tgcccgctat tagccttatc tatcatgtcc gcgcacaaaa agcgaagcgc attcgccatg ctttttaatc gttctaagtc agcgttactc aatcgcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgat tgagtaacgc tgacttagaa cgattaaaaa gcatggcgaa tgcgcttcgc tttttgtgcg cggacatgat agataaggct aatagcgggc atccgggcgt gtgcttgggg ttagctgatg tgatggtggt tttaagcttg cacctaaacc taaaccccac taaccctaaa tggctcaata gggacaggtt ggtttttagc ggagggcatg cgagcgcatt agcgtatagt ttgttgcatt tgtggggctt tgatttgagc ttagaagatt taaagcgttt caggcaatta cactctaaaa ccccaggaca ccccgaattg caccacaccg aaggcattga aatcaccaca ggccctttag ggcaaggttt tgctaacgct gtgggcttta gcatggcgag ccaatacgct caaaaccttt tggataaaga agcgatttcc cataaagtct attgcttgtg tggggatggg gatttgcaag aaggcattag ttatgagagc gcttctttgg ccgggcacct tcgccttgat aatctcattg tgatttatga cagcaaccag atcagcattg aaggcactat caatattagt tttagcgaac aggttaaaac tcgtttttta gcgcaaaatt gggaagttct agaatgcgat gggcatgatt atcaagcgat tcatgatgct ttagaagaag ccaaaaaatc ccataaaccc acgcttttaa tcgctcatac gatcattggt aagggggcta ttggtttaga gggaagtgaa aaaacgcatg gctcgccttt aaataaagaa gtgttaaaac aatccaaaga aaacgctcaa atcaatcctg atgaaagctt tatcattagc ccaaaaaaca aaatgcattt tgaagaagtg aaagttaggg gcgttagctt agaagcctta tgggagaaat ccttaagccc taaaataaaa gaaaaaatcc atgcgttaaa gaattttgat tttaacacca tccattaccc cacctttaaa aaaggcgaat ctctagccac gagagtgagt aacggcatga ttttaaacgc tatcgccaaa gaatgcgagg gctttttagg gggaagcgcg gatttagccc cctctaataa cacgcattta aaacactctg gcgatttccc tttagggcaa aacttgcatt ttgggatcag agagcatgcc atgggggcta tcactaacgc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for HepyC.01294.a.B3.GE40960

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	25kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP SMRLSALMKL KALFIFTHDS IGVGEDGATH QPIEQLSHLR ALPNFYAFRP SDAFENTACM QIALSLNAPS ALILSRQNLP VLDEVSKEQV LKGAYVKYDA KDPIITLVAS GSEVSLALES AKILERENIQ TQVISAPCFD LLIEQDESYL KELFKGKVLV IEASRAIEWY RFADKIIGMD SFGSSAKGDK LFEKFGFSVE NITAQAKRLL NA

Validated NT Sequence

atcttactca cttatgcgtt gagtaatctt ttcgcttgag cggtaatgtt ttcaacgcta aagccaaatt tttcaaagag tttatcgccc tttgctgagc tcccaaaaga atccatgcca atgattttat ccgcaaaacg ataccactct atcgcacggc tcgcttcaat cactaaaacc ttacccttaa agagttcttt aagatagctt tcatcttgct ctatcaataa atcaaagcat ggcgcgctga tgacttgcgt ttggatattt tctcgctcta aaattttagc gctctctaaa gccaaagaga cttcgctccc gctcgcaacg agcgtgataa tgggatcttt agcatcatat ttaacatacg cgccttttaa aacctgctct ttagaaacct catcaagcac gggcaaattc tggcgcgata aaataagagc gctaggagcg ttcaaactta acgctatttg catgcaagcc gtattttcaa aagcgtcgct gggtctgaaa gcataaaaat taggcaaagc gcgtaaatgg ctcaattgct ctatgggttg gtgcgtcgcc ccgtcttcgc ccacgccaat gctgtcatgc gtgaagataa aaagggcttt taatttcatt aaagcgctca aacgcatgct gggcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHPS MRLSALMKLK ALFIFTHDSI GVGEDGATHQ PIEQLSHLRA LPNFYAFRPS DAFENTACMQ IALSLNAPSA LILSRQNLPV LDEVSKEQVL KGAYVKYDAK DPIITLVASG SEVSLALESA KILERENIQT QVISAPCFDL LIEQDESYLK ELFKGKVLVI EASRAIEWYR FADKIIGMDS FGSSAKGDKL FEKFGFSVEN ITAQAKRLLN A

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcccagca tgcgtttgag cgctttaatg aaattaaaag ccctttttat cttcacgcat gacagcattg gcgtgggcga agacggggcg acgcaccaac ccatagagca attgagccat ttacgcgctt tgcctaattt ttatgctttc agacccagcg acgcttttga aaatacggct tgcatgcaaa tagcgttaag tttgaacgct cctagcgctc ttattttatc gcgccagaat ttgcccgtgc ttgatgaggt ttctaaagag caggttttaa aaggcgcgta tgttaaatat gatgctaaag atcccattat cacgctcgtt gcgagcggga gcgaagtctc tttggcttta gagagcgcta aaattttaga gcgagaaaat atccaaacgc aagtcatcag cgcgccatgc tttgatttat tgatagagca agatgaaagc tatcttaaag aactctttaa gggtaaggtt ttagtgattg aagcgagccg tgcgatagag tggtatcgtt ttgcggataa aatcattggc atggattctt ttgggagctc agcaaagggc gataaactct ttgaaaaatt tggctttagc gttgaaaaca ttaccgctca agcgaaaaga ttactcaacg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat