HepyC.01187.a
Glutamate--tRNA ligase 1 (EC 6.1.1.17) (Glutamyl-tRNA synthetase 1) (GluRS 1)
| CENTER ID: | HepyC.01187.a |
| ORGANISM: | Helicobacter pylori G27 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.01187.a.B1.GE40956 | full length | 1 | 463 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.01187.a.B1.PW38242 | full length | 1 | 463 |
Structures
6B1P
DEPOSITED: 9/18/2017
DETERMINATION: XRay
CLONE: HepyC.01187.a.B1.GE40956
PROTEIN: HepyC.01187.a.B1.PW38242
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|563041.6.peg.472 |
| UniProt: | B5Z6J9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLIVTRFAP SPTGYLHIGG LRTAIFNYLF ARANQGKFFL RIEDTDLSRN SIEAANAIIE AFKWVGLEYD GEILYQSKRF EIYKEYIQKL LDEDKAYYCY MSKDELDALR EEQKARKETP RYDNRYRDFK GTPPKGIEPV VRIKVPQNEV IGFNDGVKGE VKVNTNELDD FIIARSDGTP TYNFVVIVDD ALMGITDVIR GDDHLSNTPK QIVLYKALNF KIPNFFHVPM ILNEEGQKLS KRHGATNVMD YQEMGYLKEA LVNFLVRLGW SYQDKEIFSM QELLECFDPK DLNSSPSCFS WHKLNWLNAH YLKNQSAQKL LELLKPFSFS DLSHLNPAQL DRLLDALKER SQTLKELALK IDEVLIAPVE YEEKVFKKLN QALIMPLLEK FKLELKEANF NDESALENAM HKIIEEEKIK AGSFMQPLRL ALLGKGGGIG LKEALFILGK TESVKRIENF LKN
NT Sequence
atgagtttga tcgttacgcg cttcgccccg tcgcccactg gctacctcca cataggaggt ttaaggacag ccattttcaa ttatcttttt gcacgagcca atcaaggaaa gtttttttta cgcattgaag atacggattt gagccgtaac tctatagaag cggctaacgc cattatagag gctttcaaat gggtagggct agaatacgat ggcgaaatcc tctaccaatc caaacgcttt gagatttata aagaatacat tcaaaaactc ttagacgaag acaaagccta ttattgctac atgagcaaag atgagttgga cgctttgaga gaagagcaaa aggccaggaa agaaacccca cgctatgaca atcgttatcg tgattttaaa ggcacgcccc ctaaaggcat agagcctgtg gtaaggatta aagtcccgca aaatgaggtg attggtttta atgatggggt taaaggcgaa gtgaaagtga acaccaacga attagacgat tttattatcg ccaggagcga tgggacgccc acttataact ttgtggttat tgttgatgac gctttaatgg ggattactga tgtgattaga ggcgatgatc acctttctaa cacccctaaa caaatcgttc tttataaggc tttgaatttt aaaatcccta attttttcca tgtgccgatg attttgaacg aagaagggca aaaattaagc aaacgccatg gggccactaa tgtgatggac tatcaagaaa tgggctatct taaggaagct ttggtgaatt ttttagtgcg tttggggtgg agctatcaag ataaagagat ttttagcatg caagaattat tggaatgttt tgaccctaaa gatttaaatt cttcgcccag ttgcttcagc tggcacaagc tcaattggct caacgctcat tatttgaaaa accaaagcgc gcagaaatta ttagagcttt taaagccttt tagttttagc gatctctcgc atttaaaccc cgcccaattg gatcgcttgt tggacgccct caaagaaaga tcccaaacct taaaagaatt agcccttaaa atagatgagg ttttaatcgc ccctgtggag tatgaagaaa aggtttttaa aaaactcaat caagcgctca tcatgccctt gttagaaaag tttaagttgg aattaaaaga agccaatttc aacgatgaaa gcgcgctaga aaacgccatg cacaaaatca ttgaagaaga aaagattaaa gcgggtagtt ttatgcagcc tttaagatta gcccttttgg gtaagggggg cgggataggc cttaaagaag cgctttttat tttaggcaaa acagagagcg tcaaaagaat agaaaatttt ttgaaaaact aa
Details for HepyC.01187.a.B1.GE40956
| HARVESTED ON: | 12/20/2016 |
| SEQUENCED ON: | 12/27/2016 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMS LIVTRFAPSP TGYLHIGGLR TAIFNYLFAR ANQGKFFLRI EDTDLSRNSI EAANAIIEAF KWVGLEYDGE ILYQSKRFEI YKEYIQKLLD EDKAYYCYMS KDELDALREE QKARKETPRY DNRYRDFKGT PPKGIEPVVR IKVPQNEVIG FNDGVKGEVK VNTNELDDFI IARSDGTPTY NFVVIVDDAL MGITDVIRGD DHLSNTPKQI VLYKALNFKI PNFFHVPMIL NEEGQKLSKR HGATNVMDYQ EMGYLKEALV NFLVRLGWSY QDKEIFSMQE LLECFDPKDL NSSPSCFSWH KLNWLNAHYL KNQSAQKLLE LLKPFSFSDL SHLNPAQLDR LLDALKERSQ TLKELALKID EVLIAPVEYE EKVFKKLNQA LIMPLLEKFK LELKEANFND ESALENAMHK IIEEEKIKAG SFMQPLRLAL LGKGGGIGLK EALFILGKTE SV
Validated NT Sequence
tttgacgctc tctgttttgc ctaaaataaa aagcgcttct ttaaggccta tcccgccccc cttacccaaa agggctaatc ttaaaggctg cataaaacta cccgctttaa tcttttcttc ttcaatgatt ttgtgcatgg cgttttctag cgcgctttca tcgttgaaat tggcttcttt taattccaac ttaaactttt ctaacaaggg catgatgagc gcttgattga gttttttaaa aaccttttct tcatactcca caggggcgat taaaacctca tctattttaa gggctaattc ttttaaggtt tgggatcttt ctttgagggc gtccaacaag cgatccaatt gggcggggtt taaatgcgag agatcgctaa aactaaaagg ctttaaaagc tctaataatt tctgcgcgct ttggtttttc aaataatgag cgttgagcca attgagcttg tgccagctga agcaactggg cgaagaattt aaatctttag ggtcaaaaca ttccaataat tcttgcatgc taaaaatctc tttatcttga tagctccacc ccaaacgcac taaaaaattc accaaagctt ccttaagata gcccatttct tgatagtcca tcacattagt ggccccatgg cgtttgctta atttttgccc ttcttcgttc aaaatcatcg gcacatggaa aaaattaggg attttaaaat tcaaagcctt ataaagaacg atttgtttag gggtgttaga aaggtgatca tcgcctctaa tcacatcagt aatccccatt aaagcgtcat caacaataac cacaaagtta taagtgggcg tcccatcgct cctggcgata ataaaatcgt ctaattcgtt ggtgttcact ttcacttcgc ctttaacccc atcattaaaa ccaatcacct cattttgcgg gactttaatc cttaccacag gctctatgcc tttagggggc gtgcctttaa aatcacgata acgattgtca tagcgtgggg tttctttcct ggccttttgc tcttctctca aagcgtccaa ctcatctttg ctcatgtagc aataataggc tttgtcttcg tctaagagtt tttgaatgta ttctttataa atctcaaagc gtttggattg gtagaggatt tcgccatcgt attctagccc tacccatttg aaagcctcta taatggcgtt agccgcttct atagagttac ggctcaaatc cgtatcttca atgcgtaaaa aaaactttcc ttgattggct cgtgcaaaaa gataattgaa aatggctgtc cttaaacctc ctatgtggag gtagccagtg ggcgacgggg cgaagcgcgt aacgatcaaa ctcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMS LIVTRFAPSP TGYLHIGGLR TAIFNYLFAR ANQGKFFLRI EDTDLSRNSI EAANAIIEAF KWVGLEYDGE ILYQSKRFEI YKEYIQKLLD EDKAYYCYMS KDELDALREE QKARKETPRY DNRYRDFKGT PPKGIEPVVR IKVPQNEVIG FNDGVKGEVK VNTNELDDFI IARSDGTPTY NFVVIVDDAL MGITDVIRGD DHLSNTPKQI VLYKALNFKI PNFFHVPMIL NEEGQKLSKR HGATNVMDYQ EMGYLKEALV NFLVRLGWSY QDKEIFSMQE LLECFDPKDL NSSPSCFSWH KLNWLNAHYL KNQSAQKLLE LLKPFSFSDL SHLNPAQLDR LLDALKERSQ TLKELALKID EVLIAPVEYE EKVFKKLNQA LIMPLLEKFK LELKEANFND ESALENAMHK IIEEEKIKAG SFMQPLRLAL LGKGGGIGLK EALFILGKTE SVKRIENFLK N
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt tgatcgttac gcgcttcgcc ccgtcgccca ctggctacct ccacatagga ggtttaagga cagccatttt caattatctt tttgcacgag ccaatcaagg aaagtttttt ttacgcattg aagatacgga tttgagccgt aactctatag aagcggctaa cgccattata gaggctttca aatgggtagg gctagaatac gatggcgaaa tcctctacca atccaaacgc tttgagattt ataaagaata cattcaaaaa ctcttagacg aagacaaagc ctattattgc tacatgagca aagatgagtt ggacgctttg agagaagagc aaaaggccag gaaagaaacc ccacgctatg acaatcgtta tcgtgatttt aaaggcacgc cccctaaagg catagagcct gtggtaagga ttaaagtccc gcaaaatgag gtgattggtt ttaatgatgg ggttaaaggc gaagtgaaag tgaacaccaa cgaattagac gattttatta tcgccaggag cgatgggacg cccacttata actttgtggt tattgttgat gacgctttaa tggggattac tgatgtgatt agaggcgatg atcacctttc taacacccct aaacaaatcg ttctttataa ggctttgaat tttaaaatcc ctaatttttt ccatgtgccg atgattttga acgaagaagg gcaaaaatta agcaaacgcc atggggccac taatgtgatg gactatcaag aaatgggcta tcttaaggaa gctttggtga attttttagt gcgtttgggg tggagctatc aagataaaga gatttttagc atgcaagaat tattggaatg ttttgaccct aaagatttaa attcttcgcc cagttgcttc agctggcaca agctcaattg gctcaacgct cattatttga aaaaccaaag cgcgcagaaa ttattagagc ttttaaagcc ttttagtttt agcgatctct cgcatttaaa ccccgcccaa ttggatcgct tgttggacgc cctcaaagaa agatcccaaa ccttaaaaga attagccctt aaaatagatg aggttttaat cgcccctgtg gagtatgaag aaaaggtttt taaaaaactc aatcaagcgc tcatcatgcc cttgttagaa aagtttaagt tggaattaaa agaagccaat ttcaacgatg aaagcgcgct agaaaacgcc atgcacaaaa tcattgaaga agaaaagatt aaagcgggta gttttatgca gcctttaaga ttagcccttt tgggtaaggg gggcgggata ggccttaaag aagcgctttt tattttaggc aaaacagaga gcgtcaaaag aatagaaaat tttttgaaaa actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for HepyC.01187.a.B1.PW38242
| PURIFICATION DATe: | 4/4/2017 |
| CONCENTRATION: | 31mg/ml |
| OBSERVED MW: | 59kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS LIVTRFAPSP TGYLHIGGLR TAIFNYLFAR ANQGKFFLRI EDTDLSRNSI EAANAIIEAF KWVGLEYDGE ILYQSKRFEI YKEYIQKLLD EDKAYYCYMS KDELDALREE QKARKETPRY DNRYRDFKGT PPKGIEPVVR IKVPQNEVIG FNDGVKGEVK VNTNELDDFI IARSDGTPTY NFVVIVDDAL MGITDVIRGD DHLSNTPKQI VLYKALNFKI PNFFHVPMIL NEEGQKLSKR HGATNVMDYQ EMGYLKEALV NFLVRLGWSY QDKEIFSMQE LLECFDPKDL NSSPSCFSWH KLNWLNAHYL KNQSAQKLLE LLKPFSFSDL SHLNPAQLDR LLDALKERSQ TLKELALKID EVLIAPVEYE EKVFKKLNQA LIMPLLEKFK LELKEANFND ESALENAMHK IIEEEKIKAG SFMQPLRLAL LGKGGGIGLK EALFILGKTE SV
Validated NT Sequence
tttgacgctc tctgttttgc ctaaaataaa aagcgcttct ttaaggccta tcccgccccc cttacccaaa agggctaatc ttaaaggctg cataaaacta cccgctttaa tcttttcttc ttcaatgatt ttgtgcatgg cgttttctag cgcgctttca tcgttgaaat tggcttcttt taattccaac ttaaactttt ctaacaaggg catgatgagc gcttgattga gttttttaaa aaccttttct tcatactcca caggggcgat taaaacctca tctattttaa gggctaattc ttttaaggtt tgggatcttt ctttgagggc gtccaacaag cgatccaatt gggcggggtt taaatgcgag agatcgctaa aactaaaagg ctttaaaagc tctaataatt tctgcgcgct ttggtttttc aaataatgag cgttgagcca attgagcttg tgccagctga agcaactggg cgaagaattt aaatctttag ggtcaaaaca ttccaataat tcttgcatgc taaaaatctc tttatcttga tagctccacc ccaaacgcac taaaaaattc accaaagctt ccttaagata gcccatttct tgatagtcca tcacattagt ggccccatgg cgtttgctta atttttgccc ttcttcgttc aaaatcatcg gcacatggaa aaaattaggg attttaaaat tcaaagcctt ataaagaacg atttgtttag gggtgttaga aaggtgatca tcgcctctaa tcacatcagt aatccccatt aaagcgtcat caacaataac cacaaagtta taagtgggcg tcccatcgct cctggcgata ataaaatcgt ctaattcgtt ggtgttcact ttcacttcgc ctttaacccc atcattaaaa ccaatcacct cattttgcgg gactttaatc cttaccacag gctctatgcc tttagggggc gtgcctttaa aatcacgata acgattgtca tagcgtgggg tttctttcct ggccttttgc tcttctctca aagcgtccaa ctcatctttg ctcatgtagc aataataggc tttgtcttcg tctaagagtt tttgaatgta ttctttataa atctcaaagc gtttggattg gtagaggatt tcgccatcgt attctagccc tacccatttg aaagcctcta taatggcgtt agccgcttct atagagttac ggctcaaatc cgtatcttca atgcgtaaaa aaaactttcc ttgattggct cgtgcaaaaa gataattgaa aatggctgtc cttaaacctc ctatgtggag gtagccagtg ggcgacgggg cgaagcgcgt aacgatcaaa ctcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMS LIVTRFAPSP TGYLHIGGLR TAIFNYLFAR ANQGKFFLRI EDTDLSRNSI EAANAIIEAF KWVGLEYDGE ILYQSKRFEI YKEYIQKLLD EDKAYYCYMS KDELDALREE QKARKETPRY DNRYRDFKGT PPKGIEPVVR IKVPQNEVIG FNDGVKGEVK VNTNELDDFI IARSDGTPTY NFVVIVDDAL MGITDVIRGD DHLSNTPKQI VLYKALNFKI PNFFHVPMIL NEEGQKLSKR HGATNVMDYQ EMGYLKEALV NFLVRLGWSY QDKEIFSMQE LLECFDPKDL NSSPSCFSWH KLNWLNAHYL KNQSAQKLLE LLKPFSFSDL SHLNPAQLDR LLDALKERSQ TLKELALKID EVLIAPVEYE EKVFKKLNQA LIMPLLEKFK LELKEANFND ESALENAMHK IIEEEKIKAG SFMQPLRLAL LGKGGGIGLK EALFILGKTE SVKRIENFLK N
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTT TGATCGTTAC GCGCTTCGCC CCGTCGCCCA CTGGCTACCT CCACATAGGA GGTTTAAGGA CAGCCATTTT CAATTATCTT TTTGCACGAG CCAATCAAGG AAAGTTTTTT TTACGCATTG AAGATACGGA TTTGAGCCGT AACTCTATAG AAGCGGCTAA CGCCATTATA GAGGCTTTCA AATGGGTAGG GCTAGAATAC GATGGCGAAA TCCTCTACCA ATCCAAACGC TTTGAGATTT ATAAAGAATA CATTCAAAAA CTCTTAGACG AAGACAAAGC CTATTATTGC TACATGAGCA AAGATGAGTT GGACGCTTTG AGAGAAGAGC AAAAGGCCAG GAAAGAAACC CCACGCTATG ACAATCGTTA TCGTGATTTT AAAGGCACGC CCCCTAAAGG CATAGAGCCT GTGGTAAGGA TTAAAGTCCC GCAAAATGAG GTGATTGGTT TTAATGATGG GGTTAAAGGC GAAGTGAAAG TGAACACCAA CGAATTAGAC GATTTTATTA TCGCCAGGAG CGATGGGACG CCCACTTATA ACTTTGTGGT TATTGTTGAT GACGCTTTAA TGGGGATTAC TGATGTGATT AGAGGCGATG ATCACCTTTC TAACACCCCT AAACAAATCG TTCTTTATAA GGCTTTGAAT TTTAAAATCC CTAATTTTTT CCATGTGCCG ATGATTTTGA ACGAAGAAGG GCAAAAATTA AGCAAACGCC ATGGGGCCAC TAATGTGATG GACTATCAAG AAATGGGCTA TCTTAAGGAA GCTTTGGTGA ATTTTTTAGT GCGTTTGGGG TGGAGCTATC AAGATAAAGA GATTTTTAGC ATGCAAGAAT TATTGGAATG TTTTGACCCT AAAGATTTAA ATTCTTCGCC CAGTTGCTTC AGCTGGCACA AGCTCAATTG GCTCAACGCT CATTATTTGA AAAACCAAAG CGCGCAGAAA TTATTAGAGC TTTTAAAGCC TTTTAGTTTT AGCGATCTCT CGCATTTAAA CCCCGCCCAA TTGGATCGCT TGTTGGACGC CCTCAAAGAA AGATCCCAAA CCTTAAAAGA ATTAGCCCTT AAAATAGATG AGGTTTTAAT CGCCCCTGTG GAGTATGAAG AAAAGGTTTT TAAAAAACTC AATCAAGCGC TCATCATGCC CTTGTTAGAA AAGTTTAAGT TGGAATTAAA AGAAGCCAAT TTCAACGATG AAAGCGCGCT AGAAAACGCC ATGCACAAAA TCATTGAAGA AGAAAAGATT AAAGCGGGTA GTTTTATGCA GCCTTTAAGA TTAGCCCTTT TGGGTAAGGG GGGCGGGATA GGCCTTAAAG AAGCGCTTTT TATTTTAGGC AAAACAGAGA GCGTCAAAAG AATAGAAAAT TTTTTGAAAA ACTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT