SSGCID: Target Page

HepyC.01136.a

1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXP reductoisomerase) (EC 1.1.1.267) (1-deoxyxylulose-5-phosphate reductoisomerase) (2-C-methyl-D-erythritol 4-phosphate synthase)

CENTER ID:	HepyC.01136.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.01136.a.B1.GE40954	full length		1	366

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.223
UniProt:	B5Z9Y5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVVLGSTGSI GKNALKIAKK FGVEIEALSC GKNIALINEQ IQVFKPKKVA VLDPNDLNNL EPLGAEVFVG LEGIDAMIEE CASNLVLNAI VGVAGLRASF KSLQRNKKLA LANKESLVSA GHLLDISQIT PIDSEHFGLW ALLQNKTLKP KSLIISASGG AFRDTPLELI AIQNAQNALK HPNWSMGSKI TIDSASMVNK LFEILETYWL FGASLKIDAL IERSSIVHAL VEFEDNSVIA HLASADMKLP ISYAINPKLA SLNASIKPLD LYALSAIKFE PISMERYTLW RYKDLLLENP KLGVVLNASN EVAMKKFLNQ EIAFGGFIQI ISQALELYAK KSFKFSTLDE VLELDKEVRD RFKNYR

NT Sequence

atggttgttt taggaagcac cggctctatt gggaaaaacg ccctaaaaat cgcaaaaaaa tttggcgtag aaatagaggc cttaagctgt gggaaaaata tcgctttaat caatgagcaa atccaagttt tcaaacccaa aaaagtagcg gttttagatc ctaacgattt gaataattta gagcctttgg gcgcggaagt gtttgtaggg ctagagggca ttgatgcgat gatagaagag tgcgcctcaa atttagtcct taacgccatt gtgggcgtgg cgggattaag ggcgagcttt aaaagcttac aaaggaacaa aaaactagcc ctagcgaata aagagagctt agtgagtgct gggcatttat tagacatttc acaaatcacg cctattgaca gcgagcattt tggtttgtgg gcgttgttgc aaaacaagac tttaaagcct aaatctttaa tcattagtgc gagtgggggg gcttttaggg acacgccttt agaacttatc gctattcaaa atgcgcaaaa cgcactcaag caccctaatt ggagcatggg atctaaaatc accattgatt cagcgagcat ggtcaataag ctttttgaaa tcctagaaac ttattggctt tttggcgcgt ctttaaagat tgatgcactc attgaaagga gttctatcgt gcatgctttg gtggagtttg aagacaattc tgtcatcgcg catttagcga gcgcggacat gaaattaccc ataagctatg ccattaaccc caaattagct tccttgaacg cttccattaa gcccttagat ttatacgctt taagcgcgat taaatttgaa cccattagca tggagcgcta cactttgtgg cgctataaag acttgttatt agaaaacccc aagcttggcg tggtgctgaa tgcgagcaat gaagtggcga tgaagaagtt tttaaatcaa gaaatcgcct ttggaggctt tattcaaatc atttctcaag ccttagaatt gtatgctaaa aaatctttca agttctctac tttagatgaa gtgctagaac tggataagga agttagggat cgttttaaaa attataggta g

Details for HepyC.01136.a.B1.GE40954

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMV VLGSTGSIGK NALKIAKKFG VEIEALSCGK NIALINEQIQ VFKPKKVAVL DPNDLNNLEP LGAEVFVGLE GIDAMIEECA SNLVLNAIVG VAGLRASFKS LQRNKKLALA NKESLVSAGH LLDISQITPI DSEHFGLWAL LQNKTLKPKS LIISASGGAF RDTPLELIAI QNAQNALKHP NWSMGSKITI DSASMVNKLF EILETYWLFG ASLKIDALIE RSSIVHALVE FEDNSVIAHL ASADMKLPIS YAINPKLASL NASIKPLDLY ALSAIKFEPI SMERYTLWRY KDLLLENPKL GVVLNASNEV AMKKFLNQEI AFGGFIQIIS QALELYAKKS FKXXXLDEVL ELDKE

Validated NT Sequence

acttccttat ccagttctag cacttcatct aaagnnnnna acttgaaaga ttttttagca tacaattcta aggcttgaga aatgatttga ataaagcctc caaaggcgat ttcttgattt aaaaacttct tcatcgccac ttcattgctc gcattcagca ccacgccaag cttggggttt tctaataaca agtctttata gcgccacaaa gtgtagcgct ccatgctaat gggttcaaat ttaatcgcgc ttaaagcgta taaatctaag ggcttaatgg aagcgttcaa ggaagctaat ttggggttaa tggcatagct tatgggtaat ttcatgtccg cgctcgctaa atgcgcgatg acagaattgt cttcaaactc caccaaagca tgcacgatag aactcctttc aatgagtgca tcaatcttta aagacgcgcc aaaaagccaa taagtttcta ggatttcaaa aagcttattg accatgctcg ctgaatcaat ggtgatttta gatcccatgc tccaattagg gtgcttgagt gcgttttgcg cattttgaat agcgataagt tctaaaggcg tgtccctaaa agccccccca ctcgcactaa tgattaaaga tttaggcttt aaagtcttgt tttgcaacaa cgcccacaaa ccaaaatgct cgctgtcaat aggcgtgatt tgtgaaatgt ctaataaatg cccagcactc actaagctct ctttattcgc tagggctagt tttttgttcc tttgtaagct tttaaagctc gcccttaatc ccgccacgcc cacaatggcg ttaaggacta aatttgaggc gcactcttct atcatcgcat caatgccctc tagccctaca aacacttccg cgcccaaagg ctctaaatta ttcaaatcgt taggatctaa aaccgctact tttttgggtt tgaaaacttg gatttgctca ttgattaaag cgatattttt cccacagctt aaggcctcta tttctacgcc aaattttttt gcgattttta gggcgttttt cccaatagag ccggtgcttc ctaaaacaac catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttg ttttaggaag caccggctct attgggaaaa acgccctaaa aatcgcaaaa aaatttggcg tagaaataga ggccttaagc tgtgggaaaa atatcgcttt aatcaatgag caaatccaag ttttcaaacc caaaaaagta gcggttttag atcctaacga tttgaataat ttagagcctt tgggcgcgga agtgtttgta gggctagagg gcattgatgc gatgatagaa gagtgcgcct caaatttagt ccttaacgcc attgtgggcg tggcgggatt aagggcgagc tttaaaagct tacaaaggaa caaaaaacta gccctagcga ataaagagag cttagtgagt gctgggcatt tattagacat ttcacaaatc acgcctattg acagcgagca ttttggtttg tgggcgttgt tgcaaaacaa gactttaaag cctaaatctt taatcattag tgcgagtggg ggggctttta gggacacgcc tttagaactt atcgctattc aaaatgcgca aaacgcactc aagcacccta attggagcat gggatctaaa atcaccattg attcagcgag catggtcaat aagctttttg aaatcctaga aacttattgg ctttttggcg cgtctttaaa gattgatgca ctcattgaaa ggagttctat cgtgcatgct ttggtggagt ttgaagacaa ttctgtcatc gcgcatttag cgagcgcgga catgaaatta cccataagct atgccattaa ccccaaatta gcttccttga acgcttccat taagccctta gatttatacg ctttaagcgc gattaaattt gaacccatta gcatggagcg ctacactttg tggcgctata aagacttgtt attagaaaac cccaagcttg gcgtggtgct gaatgcgagc aatgaagtgg cgatgaagaa gtttttaaat caagaaatcg cctttggagg ctttattcaa atcatttctc aagccttaga attgtatgct aaaaaatctt tcaagttctc tactttagat gaagtgctag aactggataa ggaagttagg gatcgtttta aaaattatag gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat