SSGCID: Target Page

HepyC.00943.a

Pyridoxine 5'-phosphate synthase (PNP synthase) (EC 2.6.99.2)

CENTER ID:	HepyC.00943.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.00943.a.B1.GE40949	full length		1	262

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1647
UniProt:	B5Z9L2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFGLNIDHI VTLREVRKTY EPEILEALFI AKNTHKVDLI TIHLREDRRH IQNEDVLRLL EISPLPINIE CSINAAITDF LCSLKNKPSK VTIVPENRNE VTTEGGLDCS LKGLEEVIRA YHNKGIEVSL FIDPLKDALH FAREHQVKQV EFHTGVYANL HNALYSNANN QIHAISALKD KSPKELKEEL HNAFLQLRRM SKEAFFMGIT ACAGHGLNYT NVKELLKIPS LRELNIGHSV VSKAVLVGLE KAILEMAQLI KR

NT Sequence

atgcgttttg gattgaatat tgatcacatt gttactttaa gagaagtgag gaagacttat gagcctgaaa ttttagaagc cctattcatc gctaaaaaca cccataaagt ggatttaatc accattcatt tgagggaaga cagacgacac attcaaaatg aagatgtttt gaggctgctt gaaataagcc ctttgcctat caatattgaa tgctctatca atgcagcaat cactgatttt ttatgctctt taaaaaataa gccgagtaag gttacaatcg tgcctgaaaa cagaaatgag gttacgacag agggggggtt ggattgttca ttaaagggtt tagaagaggt gattagagcg tatcacaata aaggcattga agtgtctttg tttattgatc ctttaaaaga tgctctgcat tttgcaaggg agcatcaagt caagcaagtg gagttccaca ctggggtgta tgcgaatttg cacaacgctt tatattctaa cgctaacaat caaatccatg ccattagcgc gctcaaagac aaaagcccta aagaactgaa agaagaattg cataacgcct ttttgcaatt aagaagaatg agtaaagaag cgttttttat gggcattacg gcgtgtgcag ggcatgggtt gaattatact aatgtgaaag aattgttaaa aatcccctct ttaagagagc ttaatatcgg tcatagcgtg gtttcaaaag cggttttagt gggcttagaa aaagcgattt tagaaatggc gcaacttatt aagcgataa

Details for HepyC.00943.a.B1.GE40949

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR FGLNIDHIVT LREVRKTYEP EILEALFIAK NTHKVDLITI HLREDRRHIQ NEDVLRLLEI SPLPINIECS INAAITDFLC SLKNKPSKVT IVPENRNEVT TEGGLDCSLK GLEEVIRAYH NKGIEVSLFI DPLKDALHFA REHQVKQVEF HTGVYANLHN ALYSNANNQI HAISALKDKS PKELKEELHN AFLQLRRMSK EAFFMGITAC AGHGLNYTNV KELLKIPSLR ELNIGHSVVS KAVLVGLEKA ILEMAQLIKR

Validated NT Sequence

atcttactca cttatcgctt aataagttgc gccatttcta aaatcgcttt ttctaagccc actaaaaccg cttttgaaac cacgctatga ccgatattaa gctctcttaa agaggggatt tttaacaatt ctttcacatt agtataattc aacccatgcc ctgcacacgc cgtaatgccc ataaaaaacg cttctttact cattcttctt aattgcaaaa aggcgttatg caattcttct ttcagttctt tagggctttt gtctttgagc gcgctaatgg catggatttg attgttagcg ttagaatata aagcgttgtg caaattcgca tacaccccag tgtggaactc cacttgcttg acttgatgct cccttgcaaa atgcagagca tcttttaaag gatcaataaa caaagacact tcaatgcctt tattgtgata cgctctaatc acctcttcta aaccctttaa tgaacaatcc aaccccccct ctgtcgtaac ctcatttctg ttttcaggca cgattgtaac cttactcggc ttatttttta aagagcataa aaaatcagtg attgctgcat tgatagagca ttcaatattg ataggcaaag ggcttatttc aagcagcctc aaaacatctt cattttgaat gtgtcgtctg tcttccctca aatgaatggt gattaaatcc actttatggg tgtttttagc gatgaatagg gcttctaaaa tttcaggctc ataagtcttc ctcacttctc ttaaagtaac aatgtgatca atattcaatc caaaacgcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtt ttggattgaa tattgatcac attgttactt taagagaagt gaggaagact tatgagcctg aaattttaga agccctattc atcgctaaaa acacccataa agtggattta atcaccattc atttgaggga agacagacga cacattcaaa atgaagatgt tttgaggctg cttgaaataa gccctttgcc tatcaatatt gaatgctcta tcaatgcagc aatcactgat tttttatgct ctttaaaaaa taagccgagt aaggttacaa tcgtgcctga aaacagaaat gaggttacga cagagggggg gttggattgt tcattaaagg gtttagaaga ggtgattaga gcgtatcaca ataaaggcat tgaagtgtct ttgtttattg atcctttaaa agatgctctg cattttgcaa gggagcatca agtcaagcaa gtggagttcc acactggggt gtatgcgaat ttgcacaacg ctttatattc taacgctaac aatcaaatcc atgccattag cgcgctcaaa gacaaaagcc ctaaagaact gaaagaagaa ttgcataacg cctttttgca attaagaaga atgagtaaag aagcgttttt tatgggcatt acggcgtgtg cagggcatgg gttgaattat actaatgtga aagaattgtt aaaaatcccc tctttaagag agcttaatat cggtcatagc gtggtttcaa aagcggtttt agtgggctta gaaaaagcga ttttagaaat ggcgcaactt attaagcgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat