SSGCID: Target Page

HepyC.00914.a

Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.-)

CENTER ID:	HepyC.00914.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.00914.a.B1.GE40946	full length		1	332

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.937
UniProt:	B5Z7S5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIFINGFGR IGRCVLRAVL ERSDTNPQLE VVGINDPANW EILAYLLEHD SVHGLLFKEA HYSNCKLIIG SLEIPVFNSI KDLKGVDVIL ECSGKFLEPK TLENYLLLGA KKVLLSAPFM GEYDEKQYPT LVYGVNHFCY QNQAIVSNAS CTTNAIAPIC AILDKAFNIK EGMLTTIHSY TSDQKLIDLA HPLDKRRSRA AASNIIPTTT KAALALHKVL PNLKNKMHGH SVRVPSLDVS MIDLSLFLEK KALKEPINDL LIEASKGALK GVLEIDLKER VSSDFISNPN SVIIAPDLTF TLENMVKIMG WYDNEWGYSN RLVDMAQFMY HY

NT Sequence

atgaaaattt ttatcaatgg atttggccgc attgggagat gcgttttaag agcggtttta gagcgtagcg atacaaaccc tcaactagaa gtggtaggca tcaatgaccc tgctaattgg gaaatcctgg cttatctttt agagcatgac agcgtgcatg ggttgctttt taaagaggcg cattactcta attgcaagct tattatcggt tcgttagaaa tccctgtttt taatagtatc aaagacttga aaggcgtgga tgttatccta gagtgttcag ggaagttttt agaacctaaa acgctagaaa attacctttt gcttggggct aaaaaggttc tgttgtccgc tccctttatg ggcgaatacg atgaaaaaca ataccccact ttggtgtatg gggtcaatca tttttgctat caaaaccaag ccattgtttc taacgcctct tgcacgacta acgctatcgc gcccatttgc gcgattttag ataaagcttt taatattaaa gagggcatgc taacgaccat tcatagctac acgagcgatc aaaaactcat tgatttggcc caccctttgg ataaacggcg ctccagagcg gctgcaagca acattatccc caccaccact aaagccgctc tagccttgca taaagtgtta cccaatctca aaaacaaaat gcatgggcat agcgtgaggg tgcctagcct tgatgtgtcc atgatagatt tgagtttgtt tttagaaaaa aaggctctca aagagccgat caacgattta ttgattgaag cttcaaaagg ggctttaaaa ggcgtgttag agatagattt aaaagaaagg gtgagttctg atttcatttc taaccctaat agcgttatca tcgcgcctga tttgactttc actctggaga atatggttaa aatcatgggg tggtatgata atgaatgggg gtattctaat cgtttggtgg atatggcaca gtttatgtat cattattaa

Details for HepyC.00914.a.B1.GE40946

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMK IFINGFGRIG RCVLRAVLER SDTNPQLEVV GINDPANWEI LAYLLEHDSV HGLLFKEAHY SNCKLIIGSL EIPVFNSIKD LKGVDVILEC SGKFLEPKTL ENYLLLGAKK VLLSAPFMGE YDEKQYPTLV YGVNHFCYQN QAIVSNASCT TNAIAPICAI LDKAFNIKEG MLTTIHSYTS DQKLIDLAHP LDKRRSRAAA SNIIPTTTKA ALALHKVLPN LKNKMHGHSV RVPSLDVSMI DLSLFLEKKA LKEPINDLLI EASKGALKGV LEIDLKERVS SDFISNPNSV IIAPDLTFTL ENMVKIMGWY DNEWGYSNRL V

Validated NT Sequence

atccaccaaa cgattagaat acccccattc attatcatac caccccatga ttttaaccat attctccaga gtgaaagtca aatcaggcgc gatgataacg ctattagggt tagaaatgaa atcagaactc accctttctt ttaaatctat ctctaacacg ccttttaaag ccccttttga agcttcaatc aataaatcgt tgatcggctc tttgagagcc tttttttcta aaaacaaact caaatctatc atggacacat caaggctagg caccctcacg ctatgcccat gcattttgtt tttgagattg ggtaacactt tatgcaaggc tagagcggct ttagtggtgg tggggataat gttgcttgca gccgctctgg agcgccgttt atccaaaggg tgggccaaat caatgagttt ttgatcgctc gtgtagctat gaatggtcgt tagcatgccc tctttaatat taaaagcttt atctaaaatc gcgcaaatgg gcgcgatagc gttagtcgtg caagaggcgt tagaaacaat ggcttggttt tgatagcaaa aatgattgac cccatacacc aaagtggggt attgtttttc atcgtattcg cccataaagg gagcggacaa cagaaccttt ttagccccaa gcaaaaggta attttctagc gttttaggtt ctaaaaactt ccctgaacac tctaggataa catccacgcc tttcaagtct ttgatactat taaaaacagg gatttctaac gaaccgataa taagcttgca attagagtaa tgcgcctctt taaaaagcaa cccatgcacg ctgtcatgct ctaaaagata agccaggatt tcccaattag cagggtcatt gatgcctacc acttctagtt gagggtttgt atcgctacgc tctaaaaccg ctcttaaaac gcatctccca atgcggccaa atccattgat aaaaattttc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tttttatcaa tggatttggc cgcattggga gatgcgtttt aagagcggtt ttagagcgta gcgatacaaa ccctcaacta gaagtggtag gcatcaatga ccctgctaat tgggaaatcc tggcttatct tttagagcat gacagcgtgc atgggttgct ttttaaagag gcgcattact ctaattgcaa gcttattatc ggttcgttag aaatccctgt ttttaatagt atcaaagact tgaaaggcgt ggatgttatc ctagagtgtt cagggaagtt tttagaacct aaaacgctag aaaattacct tttgcttggg gctaaaaagg ttctgttgtc cgctcccttt atgggcgaat acgatgaaaa acaatacccc actttggtgt atggggtcaa tcatttttgc tatcaaaacc aagccattgt ttctaacgcc tcttgcacga ctaacgctat cgcgcccatt tgcgcgattt tagataaagc ttttaatatt aaagagggca tgctaacgac cattcatagc tacacgagcg atcaaaaact cattgatttg gcccaccctt tggataaacg gcgctccaga gcggctgcaa gcaacattat ccccaccacc actaaagccg ctctagcctt gcataaagtg ttacccaatc tcaaaaacaa aatgcatggg catagcgtga gggtgcctag ccttgatgtg tccatgatag atttgagttt gtttttagaa aaaaaggctc tcaaagagcc gatcaacgat ttattgattg aagcttcaaa aggggcttta aaaggcgtgt tagagataga tttaaaagaa agggtgagtt ctgatttcat ttctaaccct aatagcgtta tcatcgcgcc tgatttgact ttcactctgg agaatatggt taaaatcatg gggtggtatg ataatgaatg ggggtattct aatcgtttgg tggatatggc acagtttatg tatcattatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat