SSGCID: Target Page

HepyC.00856.a

Ferrochelatase (EC 4.99.1.1) (Heme synthase) (Protoheme ferro-lyase)

CENTER ID:	HepyC.00856.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.00856.a.B1.GE40945	full length		1	335

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1112
UniProt:	B5Z875

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLINEKLNN LENNAAKSPK EAVVLLNMGG PNSLYEVGVF LKNMFDDPFI LTIKNNFMRK MVGKMIVNSR IEKSKKIYEK LGGKSPLTPI TFALTERLNE LDPSRFYTYA MRYTPPYASM VLQDLALKEV ESLVFFSMYP QYSSTTTLSS FNDAFNALKS LETFRPKVRV IERFYADKKL NEIILNTILS ALNNRKSQDF VLIFSVHGLP KSIVDAGDTY QQECEHHVSL LKELMQQKNI PFKEVLLSYQ SKLGPMKWLE PSTEELIEKH RKSHVIIYPL AFTIDNSETL YELDMQYRLM AERLAIKEYL VCPCLNDSIE FAKFIIGLVE NLKSE

NT Sequence

atgaatttga tcaatgaaaa gctcaataat ttagaaaata acgccgcaaa atcccccaaa gaagcagtcg ttcttttgaa tatgggaggg cctaatagcc tttatgaagt gggggtgttt ttaaaaaaca tgtttgatga cccctttatc cttaccatta aaaataattt tatgcgtaaa atggtgggta aaatgattgt caatagccgc atagaaaaat ccaaaaaaat ctatgaaaaa ttaggaggca aatccccttt aacgcctatc acattcgccc ttacagagcg tttgaacgaa ttagatcctt ctcgctttta cacttatgcg atgcgttata ccccccctta tgcgtctatg gtcttgcaag atttggcctt aaaagaggta gaaagcttgg tgtttttttc catgtatcct caatattcta gcaccaccac cctttctagt ttcaatgacg cttttaacgc cctaaaatcc ctagaaactt tccgccctaa ggtgcgagtg atagaacgct tttatgctga caaaaagctt aatgaaatca ttttaaacac gattttaagc gccctaaaca accgcaaaag ccaggatttt gtcttaatct tttctgtcca tggcttacct aaaagcattg ttgatgcggg cgatacttac cagcaagaat gcgaacacca tgtgagtttg ctaaaagagt tgatgcagca aaaaaatatc ccttttaaag aagttttact ctcttaccaa tccaagctag ggcctatgaa atggctagag ccaagcactg aagagttgat agaaaagcac cgcaagtctc atgttatcat ctatcctttg gctttcacga ttgacaattc tgaaacgctc tatgaattag acatgcaata ccgcttgatg gcagagcgct tggcaattaa agaatattta gtttgcccat gcttaaacga ttccatagag tttgcaaaat ttatcattgg attagttgaa aaccttaaaa gtgagtga

Details for HepyC.00856.a.B1.GE40945

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	40kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMN LINEKLNNLE NNAAKSPKEA VVLLNMGGPN SLYEVGVFLK NMFDDPFILT IKNNFMRKMV GKMIVNSRIE KSKKIYEKLG GKSPLTPITF ALTERLNELD PSRFYTYAMR YTPPYASMVL QDLALKEVES LVFFSMYPQY SSTTTLSSFN DAFNALKSLE TFRPKVRVIE RFYADKKLNE IILNTILSAL NNRKSQDFVL IFSVHGLPKS IVDAGDTYQQ ECEHHVSLLK ELMQQKNIPF KEVLLSYQSK LGPMKWLEPS TEELIEKHRK SHVIIYPLAF TIDNSETLYE LDMQYRLMAE RLAIKEYLVC PCLNDSIEFA KFII

Validated NT Sequence

tccaatgata aattttgcaa actctatgga atcgtttaag catgggcaaa ctaaatattc tttaattgcc aagcgctctg ccatcaagcg gtattgcatg tctaattcat agagcgtttc agaattgtca atcgtgaaag ccaaaggata gatgataaca tgagacttgc ggtgcttttc tatcaactct tcagtgcttg gctctagcca tttcataggc cctagcttgg attggtaaga gagtaaaact tctttaaaag ggatattttt ttgctgcatc aactctttta gcaaactcac atggtgttcg cattcttgct ggtaagtatc gcccgcatca acaatgcttt taggtaagcc atggacagaa aagattaaga caaaatcctg gcttttgcgg ttgtttaggg cgcttaaaat cgtgtttaaa atgatttcat taagcttttt gtcagcataa aagcgttcta tcactcgcac cttagggcgg aaagtttcta gggattttag ggcgttaaaa gcgtcattga aactagaaag ggtggtggtg ctagaatatt gaggatacat ggaaaaaaac accaagcttt ctacctcttt taaggccaaa tcttgcaaga ccatagacgc ataagggggg gtataacgca tcgcataagt gtaaaagcga gaaggatcta attcgttcaa acgctctgta agggcgaatg tgataggcgt taaaggggat ttgcctccta atttttcata gatttttttg gatttttcta tgcggctatt gacaatcatt ttacccacca ttttacgcat aaaattattt ttaatggtaa ggataaaggg gtcatcaaac atgtttttta aaaacacccc cacttcataa aggctattag gccctcccat attcaaaaga acgactgctt ctttggggga ttttgcggcg ttattttcta aattattgag cttttcattg atcaaattca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt tgatcaatga aaagctcaat aatttagaaa ataacgccgc aaaatccccc aaagaagcag tcgttctttt gaatatggga gggcctaata gcctttatga agtgggggtg tttttaaaaa acatgtttga tgaccccttt atccttacca ttaaaaataa ttttatgcgt aaaatggtgg gtaaaatgat tgtcaatagc cgcatagaaa aatccaaaaa aatctatgaa aaattaggag gcaaatcccc tttaacgcct atcacattcg cccttacaga gcgtttgaac gaattagatc cttctcgctt ttacacttat gcgatgcgtt ataccccccc ttatgcgtct atggtcttgc aagatttggc cttaaaagag gtagaaagct tggtgttttt ttccatgtat cctcaatatt ctagcaccac caccctttct agtttcaatg acgcttttaa cgccctaaaa tccctagaaa ctttccgccc taaggtgcga gtgatagaac gcttttatgc tgacaaaaag cttaatgaaa tcattttaaa cacgatttta agcgccctaa acaaccgcaa aagccaggat tttgtcttaa tcttttctgt ccatggctta cctaaaagca ttgttgatgc gggcgatact taccagcaag aatgcgaaca ccatgtgagt ttgctaaaag agttgatgca gcaaaaaaat atccctttta aagaagtttt actctcttac caatccaagc tagggcctat gaaatggcta gagccaagca ctgaagagtt gatagaaaag caccgcaagt ctcatgttat catctatcct ttggctttca cgattgacaa ttctgaaacg ctctatgaat tagacatgca ataccgcttg atggcagagc gcttggcaat taaagaatat ttagtttgcc catgcttaaa cgattccata gagtttgcaa aatttatcat tggattagtt gaaaacctta aaagtgagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat