HepyC.00810.a

Flavin prenyltransferase UbiX (EC 2.5.1.129)

CENTER ID: HepyC.00810.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00810.a.B2.GE40943 mature protein 18 187
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1520
UniProt: B5Z995

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLVLGISGA SGIPLALRFL EKLPKEIEVF VVASKNAHVV ALEESNINLK NAMKDLRPSG TFFNEQDIHA SIASGSYGIH KMAIIPASMD MVAKIAHGFG GDLISRSASV MLKEKRPLLI APREMPLSAI MLENLLKLAH SNAIIAPPMM TYYTQSKTLE AMQDFLVGKW FDSLGIENDL YPRWGMN
NT Sequence
atgaaattgg ttttaggcat cagcggagcg agcgggatac ccctagcctt gcggttttta gagaaattac ccaaagaaat tgaagttttt gtcgtggcgt ctaaaaacgc gcatgtcgtg gcgttagaag aatctaatat caaccttaaa aacgccatga aagatttacg gcctagtggt acttttttta acgagcaaga cattcatgcg agtattgcct cagggagtta tggtatccat aaaatggcga tcattccagc gagtatggac atggtggcta aaatcgcgca tggctttggg ggggatttga tttctaggag cgcgtctgtg atgcttaaag aaaagcgccc cttactcatt gcccctagag aaatgccttt aagcgctatc atgttggaaa atttgctcaa actcgcccat tctaatgcga tcatcgcgcc gccgatgatg acttattaca cccagagcaa gactttagaa gcgatgcaag attttttagt ggggaagtgg tttgacagcc tagggataga aaatgactta tacccgcgat ggggaatgaa ctga
Details for HepyC.00810.a.B2.GE40943
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR FLEKLPKEIE VFVVASKNAH VVALEESNIN LKNAMKDLRP SGTFFNEQDI HASIASGSYG IHKMAIIPAS MDMVAKIAHG FGGDLISRSA SVMLKEKRPL LIAPREMPLS AIMLENLLKL AHSNAIIAPP MMTYYTQSKT LEAMQDFLVG KWFDSLGIEN DLYPRWGMN
Validated NT Sequence
atcttactca cttagttcat tccccatcgc gggtataagt cattttctat ccctaggctg tcaaaccact tccccactaa aaaatcttgc atcgcttcta aagtcttgct ctgggtgtaa taagtcatca tcggcggcgc gatgatcgca ttagaatggg cgagtttgag caaattttcc aacatgatag cgcttaaagg catttctcta ggggcaatga gtaaggggcg cttttcttta agcatcacag acgcgctcct agaaatcaaa tcccccccaa agccatgcgc gattttagcc accatgtcca tactcgctgg aatgatcgcc attttatgga taccataact ccctgaggca atactcgcat gaatgtcttg ctcgttaaaa aaagtaccac taggccgtaa atctttcatg gcgtttttaa ggttgatatt agattcttct aacgccacga catgcgcgtt tttagacgcc acgacaaaaa cttcaatttc tttgggtaat ttctctaaaa accgcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHRF LEKLPKEIEV FVVASKNAHV VALEESNINL KNAMKDLRPS GTFFNEQDIH ASIASGSYGI HKMAIIPASM DMVAKIAHGF GGDLISRSAS VMLKEKRPLL IAPREMPLSA IMLENLLKLA HSNAIIAPPM MTYYTQSKTL EAMQDFLVGK WFDSLGIEND LYPRWGMN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcggtttt tagagaaatt acccaaagaa attgaagttt ttgtcgtggc gtctaaaaac gcgcatgtcg tggcgttaga agaatctaat atcaacctta aaaacgccat gaaagattta cggcctagtg gtactttttt taacgagcaa gacattcatg cgagtattgc ctcagggagt tatggtatcc ataaaatggc gatcattcca gcgagtatgg acatggtggc taaaatcgcg catggctttg ggggggattt gatttctagg agcgcgtctg tgatgcttaa agaaaagcgc cccttactca ttgcccctag agaaatgcct ttaagcgcta tcatgttgga aaatttgctc aaactcgccc attctaatgc gatcatcgcg ccgccgatga tgacttatta cacccagagc aagactttag aagcgatgca agatttttta gtggggaagt ggtttgacag cctagggata gaaaatgact tatacccgcg atggggaatg aactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat