HepyC.00630.a

Rod shape-determining protein

CENTER ID: HepyC.00630.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00630.a.B1.GE40940 full length 1 347
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00630.a.B1.PW38437 full length 1 347

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1426
UniProt: B5Z917

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIFSKLIGLF SHDIAIDLGT ANTIVLVKGQ GIIINEPSIV AVRMGLFDSK AYDILAVGSE AKEMLGKTPN SIRAIRPMKD GVIADYDITA KMIRYFIEKA HKRKTWIRPR IMVCVPYGLT SVERNAVKES ALSAGAREVF LIEEPMAAAI GAGLPVKEPQ GSLIVDIGGG TTEIGVISLG GLVISKSIRV AGDKLDQSIV EYIRKKFNLL IGERTGEEIK IEIGCAIKLD PPLTMEVSGR DQVSGLLHTI ELSSDDVFEA IKDQVREISN ALRSVLEEVK PDLAKDIVQN GVVLTGGGAL IKGLDKYLSD MVKLPVYVGD EPLLAVAKGT GEAIQDLDLL SRVGFSE
NT Sequence
atgattttta gcaaattaat cggtttgttt tcgcatgata ttgccataga tttaggcacg gctaacacga tcgtgttagt caaagggcag ggcattatta tcaatgagcc ttctattgtg gcggtgcgca tggggttgtt tgattctaaa gcttatgata ttttggcagt ggggagcgag gctaaggaaa tgctaggcaa aacccctaac agcatcagag cgattcgccc catgaaagat ggcgtgattg ccgattatga cattaccgct aaaatgatcc gctattttat tgaaaaagcg cacaaacgaa aaacatggat ccgcccgcgc atcatggtgt gcgtgcctta tgggttgaca agcgtggaaa ggaatgcggt taaagagagc gctttgagcg cgggagcgag agaggtcttt ttgattgaag agcctatggc ggccgcgatc ggggcaggct tgccagtcaa agagccgcaa gggagtttga ttgtggatat tggcggaggc acgactgaaa ttggcgtgat cagccttggg gggttagtca tttctaaaag cattagagtg gccggagata aattggatca aagcatcgtg gaatacatcc gcaagaaatt caatttattg ataggggagc gcaccggcga agagattaag attgaaatcg gttgcgcgat caaattggat ccgcccctaa cgatggaagt gtcagggagg gatcaagtga gcgggttgtt gcacacgatt gaattgagct ctgatgatgt gtttgaagcc attaaagatc aggtgagaga aatctctaac gctttaagga gcgtgcttga agaggtgaag ccggatctag cgaaagacat tgtgcaaaat ggtgtggtgc ttaccggcgg tggggctttg attaagggct tagacaagta tttaagcgat atggttaaac tccctgtgta tgtgggcgat gagcctttat tggcggtagc caaaggcaca ggagaagcca tacaagattt ggatttactc agccgtgtgg gttttagtga ataa
Details for HepyC.00630.a.B1.GE40940
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMI FSKLIGLFSH DIAIDLGTAN TIVLVKGQGI IINEPSIVAV RMGLFDSKAY DILAVGSEAK EMLGKTPNSI RAIRPMKDGV IADYDITAKM IRYFIEKAHK RKTWIRPRIM VCVPYGLTSV ERNAVKESAL SAGAREVFLI EEPMAAAIGA GLPVKEPQGS LIVDIGGGTT EIGVISLGGL VISKSIRVAG DKLDQSIVEY IRKKFNLLIG ERTGEEIKIE IGCAIKLDPP LTMEVSGRDQ VSGLLHTIEL SSDDVFEAIK DQVREISNAL RSVLEEVKPD LAKDIVQNGV VLTGGGALIK GLDKYLSDMV KLPVYVGDEP LLAVAKGTGE AIQDLDX
Validated NT Sequence
gnnnaaatcc aaatcttgta tggcttctcc tgtgcctttg gctaccgcca ataaaggctc atcgcccaca tacacaggga gtttaaccat atcgcttaaa tacttgtcta agcccttaat caaagcccca ccgccggtaa gcaccacacc attttgcaca atgtctttcg ctagatccgg cttcacctct tcaagcacgc tccttaaagc gttagagatt tctctcacct gatctttaat ggcttcaaac acatcatcag agctcaattc aatcgtgtgc aacaacccgc tcacttgatc cctccctgac acttccatcg ttaggggcgg atccaatttg atcgcgcaac cgatttcaat cttaatctct tcgccggtgc gctcccctat caataaattg aatttcttgc ggatgtattc cacgatgctt tgatccaatt tatctccggc cactctaatg cttttagaaa tgactaaccc cccaaggctg atcacgccaa tttcagtcgt gcctccgcca atatccacaa tcaaactccc ttgcggctct ttgactggca agcctgcccc gatcgcggcc gccataggct cttcaatcaa aaagacctct ctcgctcccg cgctcaaagc gctctcttta accgcattcc tttccacgct tgtcaaccca taaggcacgc acaccatgat gcgcgggcgg atccatgttt ttcgtttgtg cgctttttca ataaaatagc ggatcatttt agcggtaatg tcataatcgg caatcacgcc atctttcatg gggcgaatcg ctctgatgct gttaggggtt ttgcctagca tttccttagc ctcgctcccc actgccaaaa tatcataagc tttagaatca aacaacccca tgcgcaccgc cacaatagaa ggctcattga taataatgcc ctgccctttg actaacacga tcgtgttagc cgtgcctaaa tctatggcaa tatcatgcga aaacaaaccg attaatttgc taaaaatcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMI FSKLIGLFSH DIAIDLGTAN TIVLVKGQGI IINEPSIVAV RMGLFDSKAY DILAVGSEAK EMLGKTPNSI RAIRPMKDGV IADYDITAKM IRYFIEKAHK RKTWIRPRIM VCVPYGLTSV ERNAVKESAL SAGAREVFLI EEPMAAAIGA GLPVKEPQGS LIVDIGGGTT EIGVISLGGL VISKSIRVAG DKLDQSIVEY IRKKFNLLIG ERTGEEIKIE IGCAIKLDPP LTMEVSGRDQ VSGLLHTIEL SSDDVFEAIK DQVREISNAL RSVLEEVKPD LAKDIVQNGV VLTGGGALIK GLDKYLSDMV KLPVYVGDEP LLAVAKGTGE AIQDLDLLSR VGFSE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattt ttagcaaatt aatcggtttg ttttcgcatg atattgccat agatttaggc acggctaaca cgatcgtgtt agtcaaaggg cagggcatta ttatcaatga gccttctatt gtggcggtgc gcatggggtt gtttgattct aaagcttatg atattttggc agtggggagc gaggctaagg aaatgctagg caaaacccct aacagcatca gagcgattcg ccccatgaaa gatggcgtga ttgccgatta tgacattacc gctaaaatga tccgctattt tattgaaaaa gcgcacaaac gaaaaacatg gatccgcccg cgcatcatgg tgtgcgtgcc ttatgggttg acaagcgtgg aaaggaatgc ggttaaagag agcgctttga gcgcgggagc gagagaggtc tttttgattg aagagcctat ggcggccgcg atcggggcag gcttgccagt caaagagccg caagggagtt tgattgtgga tattggcgga ggcacgactg aaattggcgt gatcagcctt ggggggttag tcatttctaa aagcattaga gtggccggag ataaattgga tcaaagcatc gtggaataca tccgcaagaa attcaattta ttgatagggg agcgcaccgg cgaagagatt aagattgaaa tcggttgcgc gatcaaattg gatccgcccc taacgatgga agtgtcaggg agggatcaag tgagcgggtt gttgcacacg attgaattga gctctgatga tgtgtttgaa gccattaaag atcaggtgag agaaatctct aacgctttaa ggagcgtgct tgaagaggtg aagccggatc tagcgaaaga cattgtgcaa aatggtgtgg tgcttaccgg cggtggggct ttgattaagg gcttagacaa gtatttaagc gatatggtta aactccctgt gtatgtgggc gatgagcctt tattggcggt agccaaaggc acaggagaag ccatacaaga tttggattta ctcagccgtg tgggttttag tgaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for HepyC.00630.a.B1.PW38437
PURIFICATION DATe: 2/28/2018
CONCENTRATION: 5.87mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 22
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
Protein crashes out at 5.87mg/ml
Validated AA Sequence
MAHHHHHHMI FSKLIGLFSH DIAIDLGTAN TIVLVKGQGI IINEPSIVAV RMGLFDSKAY DILAVGSEAK EMLGKTPNSI RAIRPMKDGV IADYDITAKM IRYFIEKAHK RKTWIRPRIM VCVPYGLTSV ERNAVKESAL SAGAREVFLI EEPMAAAIGA GLPVKEPQGS LIVDIGGGTT EIGVISLGGL VISKSIRVAG DKLDQSIVEY IRKKFNLLIG ERTGEEIKIE IGCAIKLDPP LTMEVSGRDQ VSGLLHTIEL SSDDVFEAIK DQVREISNAL RSVLEEVKPD LAKDIVQNGV VLTGGGALIK GLDKYLSDMV KLPVYVGDEP LLAVAKGTGE AIQDLDX
Validated NT Sequence
gnnnaaatcc aaatcttgta tggcttctcc tgtgcctttg gctaccgcca ataaaggctc atcgcccaca tacacaggga gtttaaccat atcgcttaaa tacttgtcta agcccttaat caaagcccca ccgccggtaa gcaccacacc attttgcaca atgtctttcg ctagatccgg cttcacctct tcaagcacgc tccttaaagc gttagagatt tctctcacct gatctttaat ggcttcaaac acatcatcag agctcaattc aatcgtgtgc aacaacccgc tcacttgatc cctccctgac acttccatcg ttaggggcgg atccaatttg atcgcgcaac cgatttcaat cttaatctct tcgccggtgc gctcccctat caataaattg aatttcttgc ggatgtattc cacgatgctt tgatccaatt tatctccggc cactctaatg cttttagaaa tgactaaccc cccaaggctg atcacgccaa tttcagtcgt gcctccgcca atatccacaa tcaaactccc ttgcggctct ttgactggca agcctgcccc gatcgcggcc gccataggct cttcaatcaa aaagacctct ctcgctcccg cgctcaaagc gctctcttta accgcattcc tttccacgct tgtcaaccca taaggcacgc acaccatgat gcgcgggcgg atccatgttt ttcgtttgtg cgctttttca ataaaatagc ggatcatttt agcggtaatg tcataatcgg caatcacgcc atctttcatg gggcgaatcg ctctgatgct gttaggggtt ttgcctagca tttccttagc ctcgctcccc actgccaaaa tatcataagc tttagaatca aacaacccca tgcgcaccgc cacaatagaa ggctcattga taataatgcc ctgccctttg actaacacga tcgtgttagc cgtgcctaaa tctatggcaa tatcatgcga aaacaaaccg attaatttgc taaaaatcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMI FSKLIGLFSH DIAIDLGTAN TIVLVKGQGI IINEPSIVAV RMGLFDSKAY DILAVGSEAK EMLGKTPNSI RAIRPMKDGV IADYDITAKM IRYFIEKAHK RKTWIRPRIM VCVPYGLTSV ERNAVKESAL SAGAREVFLI EEPMAAAIGA GLPVKEPQGS LIVDIGGGTT EIGVISLGGL VISKSIRVAG DKLDQSIVEY IRKKFNLLIG ERTGEEIKIE IGCAIKLDPP LTMEVSGRDQ VSGLLHTIEL SSDDVFEAIK DQVREISNAL RSVLEEVKPD LAKDIVQNGV VLTGGGALIK GLDKYLSDMV KLPVYVGDEP LLAVAKGTGE AIQDLDLLSR VGFSE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTT TTAGCAAATT AATCGGTTTG TTTTCGCATG ATATTGCCAT AGATTTAGGC ACGGCTAACA CGATCGTGTT AGTCAAAGGG CAGGGCATTA TTATCAATGA GCCTTCTATT GTGGCGGTGC GCATGGGGTT GTTTGATTCT AAAGCTTATG ATATTTTGGC AGTGGGGAGC GAGGCTAAGG AAATGCTAGG CAAAACCCCT AACAGCATCA GAGCGATTCG CCCCATGAAA GATGGCGTGA TTGCCGATTA TGACATTACC GCTAAAATGA TCCGCTATTT TATTGAAAAA GCGCACAAAC GAAAAACATG GATCCGCCCG CGCATCATGG TGTGCGTGCC TTATGGGTTG ACAAGCGTGG AAAGGAATGC GGTTAAAGAG AGCGCTTTGA GCGCGGGAGC GAGAGAGGTC TTTTTGATTG AAGAGCCTAT GGCGGCCGCG ATCGGGGCAG GCTTGCCAGT CAAAGAGCCG CAAGGGAGTT TGATTGTGGA TATTGGCGGA GGCACGACTG AAATTGGCGT GATCAGCCTT GGGGGGTTAG TCATTTCTAA AAGCATTAGA GTGGCCGGAG ATAAATTGGA TCAAAGCATC GTGGAATACA TCCGCAAGAA ATTCAATTTA TTGATAGGGG AGCGCACCGG CGAAGAGATT AAGATTGAAA TCGGTTGCGC GATCAAATTG GATCCGCCCC TAACGATGGA AGTGTCAGGG AGGGATCAAG TGAGCGGGTT GTTGCACACG ATTGAATTGA GCTCTGATGA TGTGTTTGAA GCCATTAAAG ATCAGGTGAG AGAAATCTCT AACGCTTTAA GGAGCGTGCT TGAAGAGGTG AAGCCGGATC TAGCGAAAGA CATTGTGCAA AATGGTGTGG TGCTTACCGG CGGTGGGGCT TTGATTAAGG GCTTAGACAA GTATTTAAGC GATATGGTTA AACTCCCTGT GTATGTGGGC GATGAGCCTT TATTGGCGGT AGCCAAAGGC ACAGGAGAAG CCATACAAGA TTTGGATTTA CTCAGCCGTG TGGGTTTTAG TGAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT