SSGCID: Target Page

HepyC.00617.a

Glyceraldehyde-3-phosphate dehydrogenase

CENTER ID:	HepyC.00617.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.00617.a.B1.GE40939	full length		1	330

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.00617.a.B1.PW38425	full length		1	330

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.1401
UniProt:	B5Z8Z3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPIRIAINGT GRIGLCAIRV ASQRKDIEIV AINSTAELET LLHLIRHDSV HGHFEAKLNA NRTLNIGHSK NILVLSERDI NKLDFSVANA EIIIECTGKF NSLEASSAHL KNSVKKVIIS APAQNTPTFV YGVNHKNYHN ESVISNASCT TNATAPLLKI LDEAFKVENA LLTTIHSYTN DQNLLDTKHK DIRRARAAGL NLIPTSTGVS KAISLVLPHL GPKVTGLAIR VPTPNVSLVD LSLSFKKSVS KASVQHALKD ACKHAFKGVV GIDEERLVSS DFISSPFSAI VIGDQIMTIG EKNAKVLAWY DNEMGYSERL IDMAQYIAQN

NT Sequence

atgccaatta gaatcgctat caatgggact gggcgcatcg gtttatgcgc tataagagtc gctagccaaa gaaaggatat agaaattgtc gctatcaatt ctaccgctga attagaaact cttttgcatt tgatccgcca tgacagcgtg catgggcatt ttgaagccaa attaaacgcc aatagaaccc taaatatcgg gcatagtaaa aatattttag tgttgagcga gcgagacatt aacaagcttg atttttctgt cgctaacgca gaaatcatca tagaatgcac cgggaaattc aattccctag aagcttcaag cgcccatctt aaaaacagcg tgaaaaaagt catcatctcc gctcccgcgc aaaatacgcc cacctttgtc tatggggtga atcataagaa ttaccataac gaaagcgtga tttctaacgc ctcttgcacg actaacgcta ccgctcctct attaaaaatc ttagatgaag cctttaaagt agaaaacgcg cttttaacca ccatccacag ctacactaac gatcaaaacc tcttagacac caagcacaaa gacatccgcc gcgctagagc ggctggttta aaccttatcc ccacaagcac cggcgtgagc aaagccattt cgctagtctt accgcattta ggccctaaag ttacaggcct tgcgattaga gtgcctaccc ctaatgtgag cttggtggat ctatctttaa gctttaaaaa atccgtgagt aaagcgagcg ttcagcatgc gcttaaagac gcttgtaagc atgcctttaa aggggttgtg ggtattgatg aagaaaggct tgtttcaagc gattttattt cttcgccttt tagcgcgatc gtgattggtg atcaaatcat gacaataggc gaaaaaaatg ctaaagtatt ggcatggtat gataatgaga tgggttatag cgagcgcttg atagacatgg cacaatatat agcacaaaat taa

Details for HepyC.00617.a.B1.GE40939

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP IXIAINGTGR IGLCAIRVAS QRKDIEIVAI NSTAELETLL HLIRHDSVHG HFEAKLNAXR TLNIGHSKNI LVLSERDINK LDFSVANAEI IIECTGKFNS LEASSAHLKN SVKKVIISAP AQNTPTFVYG VNHKNYHNES VISNASCTTN ATAPLLKILD EAFKVENALL TTIHSYTNDQ NLLDTKHKDI RRARAAGLNL IPTSTGVSKA ISLVLPHLGP KVTGLAIRVP TPNVSLVDLS LSFKKSVSKA SVQHALKDAC KHAFKGVVGI DEERLVSSDF ISSPFSAIVI GDQIMTIGEK NAKVLAWYDN EMGYSERLI

Validated NT Sequence

gtctatcaag cgctcgctat aacccatctc attatcatac catgccaata ctttagcatt tttttcgcct attgtcatga tttgatcacc aatcacgatc gcgctaaaag gcgaagaaat aaaatcgctt gaaacaagcc tttcttcatc aatacccaca acccctttaa aggcatgctt acaagcgtct ttaagcgcat gctgaacgct cgctttactc acggattttt taaagcttaa agatagatcc accaagctca cattaggggt aggcactcta atcgcaaggc ctgtaacttt agggcctaaa tgcggtaaga ctagcgaaat ggctttgctc acgccggtgc ttgtggggat aaggtttaaa ccagccgctc tagcgcggcg gatgtctttg tgcttggtgt ctaagaggtt ttgatcgtta gtgtagctgt ggatggtggt taaaagcgcg ttttctactt taaaggcttc atctaagatt tttaatagag gagcggtagc gttagtcgtg caagaggcgt tagaaatcac gctttcgtta tggtaattct tatgattcac cccatagaca aaggtgggcg tattttgcgc gggagcggag atgatgactt ttttcacgct gtttttaaga tgggcgcttg aagcttctag ggaattgaat ttcccggtgc attctatgat gatttctgcg ttagcgacag aaaaatcaag cttgttaatg tctcgctcgc tcaacactaa aatattttta ctatgcccga tatttagggt tctatnngcg tttaatttgg cttcaaaatg cccatgcacg ctgtcatggc ggatcaaatg caaaagagtt tctaattcag cggtagaatt gatagcgaca atttctatat cctttctttg gctagcgact cttatagcgc ataaaccgat gcgcccagtc ccattgatag cgatnnnaat tggcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMP IRIAINGTGR IGLCAIRVAS QRKDIEIVAI NSTAELETLL HLIRHDSVHG HFEAKLNANR TLNIGHSKNI LVLSERDINK LDFSVANAEI IIECTGKFNS LEASSAHLKN SVKKVIISAP AQNTPTFVYG VNHKNYHNES VISNASCTTN ATAPLLKILD EAFKVENALL TTIHSYTNDQ NLLDTKHKDI RRARAAGLNL IPTSTGVSKA ISLVLPHLGP KVTGLAIRVP TPNVSLVDLS LSFKKSVSKA SVQHALKDAC KHAFKGVVGI DEERLVSSDF ISSPFSAIVI GDQIMTIGEK NAKVLAWYDN EMGYSERLID MAQYIAQN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa ttagaatcgc tatcaatggg actgggcgca tcggtttatg cgctataaga gtcgctagcc aaagaaagga tatagaaatt gtcgctatca attctaccgc tgaattagaa actcttttgc atttgatccg ccatgacagc gtgcatgggc attttgaagc caaattaaac gccaatagaa ccctaaatat cgggcatagt aaaaatattt tagtgttgag cgagcgagac attaacaagc ttgatttttc tgtcgctaac gcagaaatca tcatagaatg caccgggaaa ttcaattccc tagaagcttc aagcgcccat cttaaaaaca gcgtgaaaaa agtcatcatc tccgctcccg cgcaaaatac gcccaccttt gtctatgggg tgaatcataa gaattaccat aacgaaagcg tgatttctaa cgcctcttgc acgactaacg ctaccgctcc tctattaaaa atcttagatg aagcctttaa agtagaaaac gcgcttttaa ccaccatcca cagctacact aacgatcaaa acctcttaga caccaagcac aaagacatcc gccgcgctag agcggctggt ttaaacctta tccccacaag caccggcgtg agcaaagcca tttcgctagt cttaccgcat ttaggcccta aagttacagg ccttgcgatt agagtgccta cccctaatgt gagcttggtg gatctatctt taagctttaa aaaatccgtg agtaaagcga gcgttcagca tgcgcttaaa gacgcttgta agcatgcctt taaaggggtt gtgggtattg atgaagaaag gcttgtttca agcgatttta tttcttcgcc ttttagcgcg atcgtgattg gtgatcaaat catgacaata ggcgaaaaaa atgctaaagt attggcatgg tatgataatg agatgggtta tagcgagcgc ttgatagaca tggcacaata tatagcacaa aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for HepyC.00617.a.B1.PW38425

PURIFICATION DATe:	1/29/2018
CONCENTRATION:	13.44mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAA TTAGAATCGC TATCAATGGG ACTGGGCGCA TCGGTTTATG CGCTATAAGA GTCGCTAGCC AAAGAAAGGA TATAGAAATT GTCGCTATCA ATTCTACCGC TGAATTAGAA ACTCTTTTGC ATTTGATCCG CCATGACAGC GTGCATGGGC ATTTTGAAGC CAAATTAAAC GCCAATAGAA CCCTAAATAT CGGGCATAGT AAAAATATTT TAGTGTTGAG CGAGCGAGAC ATTAACAAGC TTGATTTTTC TGTCGCTAAC GCAGAAATCA TCATAGAATG CACCGGGAAA TTCAATTCCC TAGAAGCTTC AAGCGCCCAT CTTAAAAACA GCGTGAAAAA AGTCATCATC TCCGCTCCCG CGCAAAATAC GCCCACCTTT GTCTATGGGG TGAATCATAA GAATTACCAT AACGAAAGCG TGATTTCTAA CGCCTCTTGC ACGACTAACG CTACCGCTCC TCTATTAAAA ATCTTAGATG AAGCCTTTAA AGTAGAAAAC GCGCTTTTAA CCACCATCCA CAGCTACACT AACGATCAAA ACCTCTTAGA CACCAAGCAC AAAGACATCC GCCGCGCTAG AGCGGCTGGT TTAAACCTTA TCCCCACAAG CACCGGCGTG AGCAAAGCCA TTTCGCTAGT CTTACCGCAT TTAGGCCCTA AAGTTACAGG CCTTGCGATT AGAGTGCCTA CCCCTAATGT GAGCTTGGTG GATCTATCTT TAAGCTTTAA AAAATCCGTG AGTAAAGCGA GCGTTCAGCA TGCGCTTAAA GACGCTTGTA AGCATGCCTT TAAAGGGGTT GTGGGTATTG ATGAAGAAAG GCTTGTTTCA AGCGATTTTA TTTCTTCGCC TTTTAGCGCG ATCGTGATTG GTGATCAAAT CATGACAATA GGCGAAAAAA ATGCTAAAGT ATTGGCATGG TATGATAATG AGATGGGTTA TAGCGAGCGC TTGATAGACA TGGCACAATA TATAGCACAA AATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT