HepyC.00571.a

tRNA modification GTPase MnmE (EC 3.6.-.-)

CENTER ID: HepyC.00571.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00571.a.B1.GE40937 full length 1 404
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1493
UniProt: B5Z969

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRDIFSNGVL LDKALVIYFK APYSFTGEDV CEIQCHGSPL LAQNILQACL NLGARLAKAG EFSKKAFLNH KMDLSEIEAS VQLILCEDES VLNALARQLK GELKIFIEEA RNNLLKLLAS SEVLIDYSEE DIPSDFLDGV SQNLEKQIAS FKDLLDFSNA QKQRNKGHAL SIVGKPNAGK SSLLNAMLLE ERALVSDIKG TTRDTIEEVI ELQGHKVRLI DTAGIRESAD KIERLGIEKS LKSLENCDII LGVFDLSKPL EKEDFTIIDA LNRAKKPCIV VLNKNDLAPK LELEVLKSYL KIPYALLETN TLNSRACLKD LSQKISEFFP KLDTQNKLLL TSLAQKTALE NAIIELQNAK NHLETLELFS YHILSAIENL NLLTRPYETS QMLDSMFSEF CLGK
NT Sequence
gtgcgcgata ttttttctaa tggcgtttta ttagacaaag cgttagtcat ttatttcaaa gccccctata gtttcaccgg tgaagatgtg tgcgaaatcc aatgccatgg aagccccctt ttagcgcaaa atatcttaca agcttgcttg aatctagggg ctaggcttgc taaagcgggg gaatttagca aaaaagcctt tttaaaccat aaaatggatt tgagcgagat tgaagcgagc gttcagctca ttctttgtga agatgagagc gttttaaacg ctctagccag gcagcttaag ggcgagttga aaatttttat tgaagaagct aggaataatc ttttaaagct tttggcgagc tcagaagttt tgattgatta tagcgaagaa gacattccta gcgatttttt agatggagtg tctcaaaatt tagaaaaaca aatcgcctct tttaaagatt tattggattt ttctaacgcg caaaaacaaa ggaataaagg gcatgcctta agcattgttg gcaaacccaa tgccggcaaa agttctttat taaacgccat gcttttagaa gaaagggctt tggtgagcga tattaaaggc acaacaagag acaccataga agaagtcatt gaattacaag ggcataaggt gcgcttgatt gatacggccg gtattagaga gagcgcggat aaaatagagc gtttagggat tgaaaaaagc cttaaaagtt tagaaaattg cgacattatt ttaggcgtgt ttgatctttc taaaccccta gaaaaagaag attttactat cattgacgcc cttaatcgcg ctaaaaagcc ttgcattgtt gttttgaata aaaacgattt agcgcccaaa ctagagcttg aagttttaaa atcttatctt aaaatccctt atgctttact agaaaccaac accctaaatt ccagggcttg cttgaaagat ttgagccaaa aaatcagtga gtttttcccc aaactagaca ctcaaaacaa gctcttactc acttccctag cccaaaaaac cgccctagaa aacgccatta ttgaattgca aaacgctaaa aaccatttag aaactttaga gcttttttct tatcatattt taagcgcgat agaaaacttg aacttgctca cccgccctta tgaaaccagc caaatgcttg atagcatgtt cagcgaattt tgcttaggca aatga
Details for HepyC.00571.a.B1.GE40937
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV RDIFSNGVLL DKALVIYFKA PYSFTGEDVC EIQCHGSPLL AQNILQACLN LGARLAKAGE FSKKAFLNHK MDLSEIEASV QLILCEDESV LNALARQLKG ELKIFIEEAR NNLLKLLASS EVLIDYSEED IPSDFLDGVS QNLEKQIASF KDLLDFSNAQ KQRNKGHALS IVGKPNAGKS SLLNAMLLEE RALVSDIKGT TRDTIEEVIE LQGHKVRLID TAGIRESADK IERLGIEKSL KSLENCDIIL GVFDLSKPLE KEDFTIIDAL NRAKKPCIVV LNKNDLAPKL ELEVLKSYLK IPYALLETNT LNSRACLKDL SQKISEFFPK LDTQNKLLLT SLAQKTALEN AIIELQNAKN HLETLELFSY HILSAIENLN LLTRPYETSQ MLDS
Validated NT Sequence
atgctatcaa gcatttggct ggtttcataa gggcgggtga gcaagttcaa gttttctatc gcgcttaaaa tatgataaga aaaaagctct aaagtttcta aatggttttt agcgttttgc aattcaataa tggcgttttc tagggcggtt ttttgggcta gggaagtgag taagagcttg ttttgagtgt ctagtttggg gaaaaactca ctgatttttt ggctcaaatc tttcaagcaa gccctggaat ttagggtgtt ggtttctagt aaagcataag ggattttaag ataagatttt aaaacttcaa gctctagttt gggcgctaaa tcgtttttat tcaaaacaac aatgcaaggc tttttagcgc gattaagggc gtcaatgata gtaaaatctt ctttttctag gggtttagaa agatcaaaca cgcctaaaat aatgtcgcaa ttttctaaac ttttaaggct tttttcaatc cctaaacgct ctattttatc cgcgctctct ctaataccgg ccgtatcaat caagcgcacc ttatgccctt gtaattcaat gacttcttct atggtgtctc ttgttgtgcc tttaatatcg ctcaccaaag ccctttcttc taaaagcatg gcgtttaata aagaactttt gccggcattg ggtttgccaa caatgcttaa ggcatgccct ttattccttt gtttttgcgc gttagaaaaa tccaataaat ctttaaaaga ggcgatttgt ttttctaaat tttgagacac tccatctaaa aaatcgctag gaatgtcttc ttcgctataa tcaatcaaaa cttctgagct cgccaaaagc tttaaaagat tattcctagc ttcttcaata aaaattttca actcgccctt aagctgcctg gctagagcgt ttaaaacgct ctcatcttca caaagaatga gctgaacgct cgcttcaatc tcgctcaaat ccattttatg gtttaaaaag gcttttttgc taaattcccc cgctttagca agcctagccc ctagattcaa gcaagcttgt aagatatttt gcgctaaaag ggggcttcca tggcattgga tttcgcacac atcttcaccg gtgaaactat agggggcttt gaaataaatg actaacgctt tgtctaataa aacgccatta gaaaaaatat cgcgcaccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMR DIFSNGVLLD KALVIYFKAP YSFTGEDVCE IQCHGSPLLA QNILQACLNL GARLAKAGEF SKKAFLNHKM DLSEIEASVQ LILCEDESVL NALARQLKGE LKIFIEEARN NLLKLLASSE VLIDYSEEDI PSDFLDGVSQ NLEKQIASFK DLLDFSNAQK QRNKGHALSI VGKPNAGKSS LLNAMLLEER ALVSDIKGTT RDTIEEVIEL QGHKVRLIDT AGIRESADKI ERLGIEKSLK SLENCDIILG VFDLSKPLEK EDFTIIDALN RAKKPCIVVL NKNDLAPKLE LEVLKSYLKI PYALLETNTL NSRACLKDLS QKISEFFPKL DTQNKLLLTS LAQKTALENA IIELQNAKNH LETLELFSYH ILSAIENLNL LTRPYETSQM LDSMFSEFCL GK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgcg atattttttc taatggcgtt ttattagaca aagcgttagt catttatttc aaagccccct atagtttcac cggtgaagat gtgtgcgaaa tccaatgcca tggaagcccc cttttagcgc aaaatatctt acaagcttgc ttgaatctag gggctaggct tgctaaagcg ggggaattta gcaaaaaagc ctttttaaac cataaaatgg atttgagcga gattgaagcg agcgttcagc tcattctttg tgaagatgag agcgttttaa acgctctagc caggcagctt aagggcgagt tgaaaatttt tattgaagaa gctaggaata atcttttaaa gcttttggcg agctcagaag ttttgattga ttatagcgaa gaagacattc ctagcgattt tttagatgga gtgtctcaaa atttagaaaa acaaatcgcc tcttttaaag atttattgga tttttctaac gcgcaaaaac aaaggaataa agggcatgcc ttaagcattg ttggcaaacc caatgccggc aaaagttctt tattaaacgc catgctttta gaagaaaggg ctttggtgag cgatattaaa ggcacaacaa gagacaccat agaagaagtc attgaattac aagggcataa ggtgcgcttg attgatacgg ccggtattag agagagcgcg gataaaatag agcgtttagg gattgaaaaa agccttaaaa gtttagaaaa ttgcgacatt attttaggcg tgtttgatct ttctaaaccc ctagaaaaag aagattttac tatcattgac gcccttaatc gcgctaaaaa gccttgcatt gttgttttga ataaaaacga tttagcgccc aaactagagc ttgaagtttt aaaatcttat cttaaaatcc cttatgcttt actagaaacc aacaccctaa attccagggc ttgcttgaaa gatttgagcc aaaaaatcag tgagtttttc cccaaactag acactcaaaa caagctctta ctcacttccc tagcccaaaa aaccgcccta gaaaacgcca ttattgaatt gcaaaacgct aaaaaccatt tagaaacttt agagcttttt tcttatcata ttttaagcgc gatagaaaac ttgaacttgc tcacccgccc ttatgaaacc agccaaatgc ttgatagcat gttcagcgaa ttttgcttag gcaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat