HepyC.00545.a

Threonine synthase

CENTER ID: HepyC.00545.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00545.a.B1.GE40936 full length 1 484
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00545.a.B1.PW38241 full length 1 484

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.104
UniProt: B5Z9M9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPFVPTRSLK EKKIDFIEAI LNPNAPKGGL YTLENFETLE WQDCLNLSYN ELVECVFERL GLEISKGLLT SALKRYENFD NPKNPAPIFA LNERLFVQEL YHGPSLAFKD MALQPLASLF SNLAVGKNEK YLMLVSTSGD TGPATLESLA GMPNVFVVCL YPKDGTSLVQ KLQMVTQSAN NLKVFGISGD FDDAQNALKN LLKDDDFNEA LKTHQLKLSV ANSVNFGRIA FQIVYHIWGF LELYKKGAIN SKEKITLAIP SGNFGNALGA FYAKKMGLNI AKIKVVTNSN DVLREFIETG RYDLTHRSLK QTYSPAMDIL KSSNVERVLF SLFGFERTLE LMQALEEEKF YALKPKELAL LQEHFSCASC SDEACLKTIQ EVYAEHQYLI DPHTATALNA SLKTNEKTLV SATASYEKFP KTTLLALNEQ KKDDKAALEA LKNSYNTPDS QRLDDLFERG IKHQEVLKLN EIKSSILLWL ESLH
NT Sequence
atgccttttg tccccacgcg ctctttgaaa gaaaaaaaga ttgattttat tgaagcaata ttaaacccca acgcccccaa aggcgggctt tacactttag aaaattttga aacattagaa tggcaagatt gtctgaatct gagctataac gagctagtgg aatgcgtgtt tgagcggttg ggtttagaga tttctaaggg tttattgaca agcgctttaa aacgctatga aaattttgat aaccctaaaa atccagcccc tatttttgcg ctcaatgaaa ggctttttgt ccaagagctc taccatgggc ctagtttggc gtttaaagac atggcgttgc agcctttagc gagcttgttt tctaacctag cggtaggaaa aaatgaaaag tatttaatgc tcgtttccac gagtggcgat actggccctg cgactttaga aagtttggcg ggcatgccta atgtttttgt ggtgtgttta taccccaaag acggcacgag tttagtccaa aaactccaaa tggtaaccca aagcgctaac aatttaaaag tctttgggat cagtggggat tttgatgatg cgcaaaacgc actcaaaaac cttttaaaag acgatgattt taacgaagcc ctaaaaacgc accaattgaa attaagcgtg gctaattccg tgaattttgg gcgcatcgct tttcaaatcg tctatcatat ttggggcttt ttggaattgt ataaaaaagg cgcgatcaat tctaaagaaa aaatcaccct agccatacct agcggtaatt ttgggaacgc tttaggggcg ttttatgcca aaaaaatggg cttgaatatt gctaaaatca aggtggtaac caacagcaat gatgttttaa gggagtttat agaaacaggg cgttacgatt taacccatcg ttctttaaag caaacttact cgccggctat ggatatttta aaaagctcta acgtggaaag ggtgcttttt agcctttttg ggtttgaacg cacactagaa ttgatgcaag ctttagaaga agagaaattt tacgccttaa aacctaaaga attagccctt ttacaggagc atttttcttg tgcgagctgc tcagatgaag cctgtttgaa gacgatccaa gaagtttatg cagagcacca atacctgatt gacccccaca ccgccaccgc tttaaacgcc agcttgaaaa ccaatgaaaa aacccttgtt tctgccaccg cctcttatga aaaattccct aaaaccaccc ttttagccct aaacgagcaa aaaaaagacg ataaagccgc cctagaagca ctaaaaaaca gctacaatac cccggatagc caacgccttg atgatttatt tgaaagagga atcaagcacc aagaagtttt aaaactcaat gagatcaaat cttctattct tttgtggcta gaaagcctac attaa
Details for HepyC.00545.a.B1.GE40936
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 55kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMP FVPTRSLKEK KIDFIEAILN PNAPKGGLYT LENFETLEWQ DCLNLSYNEL VECVFERLGL EISKGLLTSA LKRYENFDNP KNPAPIFALN ERLFVQELYH GPSLAFKDMA LQPLASLFSN LAVGKNEKYL MLVSTSGDTG PATLESLAGM PNVFVVCLYP KDGTSLVQKL QMVTQSANNL KVFGISGDFD DAQNALKNLL KDDDFNEALK THQLKLSVAN SVNFGRIAFQ IVYHIWGFLE LYKKGAINSK EKITLAIPSG NFGNALGAFY AKKMGLNIAK IKVVTNSNDV LREFIETGRY DLTHRSLKQT YSPAMDILKS SNVERVLFSL FGFERTLELM QALEEEKFYA LKPKELALLQ EHFSCASCSD EACLKTIQEV YAEHQYLIDP HTATALNASL KTNEKTLVSA TASYEKFPKT TLLALNEQKK DDKAALEALK NSYNTPDSQR LDDLFERGIK HQEVLKLNEI KSS
Validated NT Sequence
tagaagattt gatctcattg agttttaaaa cttcttggtg cttgattcct ctttcaaata aatcatcaag gcgttggcta tccggggtat tgtagctgtt ttttagtgct tctagggcgg ctttatcgtc ttttttttgc tcgtttaggg ctaaaagggt ggttttaggg aatttttcat aagaggcggt ggcagaaaca agggtttttt cattggtttt caagctggcg tttaaagcgg tggcggtgtg ggggtcaatc aggtattggt gctctgcata aacttcttgg atcgtcttca aacaggcttc atctgagcag ctcgcacaag aaaaatgctc ctgtaaaagg gctaattctt taggttttaa ggcgtaaaat ttctcttctt ctaaagcttg catcaattct agtgtgcgtt caaacccaaa aaggctaaaa agcacccttt ccacgttaga gctttttaaa atatccatag ccggcgagta agtttgcttt aaagaacgat gggttaaatc gtaacgccct gtttctataa actcccttaa aacatcattg ctgttggtta ccaccttgat tttagcaata ttcaagccca tttttttggc ataaaacgcc cctaaagcgt tcccaaaatt accgctaggt atggctaggg tgattttttc tttagaattg atcgcgcctt ttttatacaa ttccaaaaag ccccaaatat gatagacgat ttgaaaagcg atgcgcccaa aattcacgga attagccacg cttaatttca attggtgcgt ttttagggct tcgttaaaat catcgtcttt taaaaggttt ttgagtgcgt tttgcgcatc atcaaaatcc ccactgatcc caaagacttt taaattgtta gcgctttggg ttaccatttg gagtttttgg actaaactcg tgccgtcttt ggggtataaa cacaccacaa aaacattagg catgcccgcc aaactttcta aagtcgcagg gccagtatcg ccactcgtgg aaacgagcat taaatacttt tcattttttc ctaccgctag gttagaaaac aagctcgcta aaggctgcaa cgccatgtct ttaaacgcca aactaggccc atggtagagc tcttggacaa aaagcctttc attgagcgca aaaatagggg ctggattttt agggttatca aaattttcat agcgttttaa agcgcttgtc aataaaccct tagaaatctc taaacccaac cgctcaaaca cgcattccac tagctcgtta tagctcagat tcagacaatc ttgccattct aatgtttcaa aattttctaa agtgtaaagc ccgcctttgg gggcgttggg gtttaatatt gcttcaataa aatcaatctt tttttctttc aaagagcgcg tggggacaaa aggcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMP FVPTRSLKEK KIDFIEAILN PNAPKGGLYT LENFETLEWQ DCLNLSYNEL VECVFERLGL EISKGLLTSA LKRYENFDNP KNPAPIFALN ERLFVQELYH GPSLAFKDMA LQPLASLFSN LAVGKNEKYL MLVSTSGDTG PATLESLAGM PNVFVVCLYP KDGTSLVQKL QMVTQSANNL KVFGISGDFD DAQNALKNLL KDDDFNEALK THQLKLSVAN SVNFGRIAFQ IVYHIWGFLE LYKKGAINSK EKITLAIPSG NFGNALGAFY AKKMGLNIAK IKVVTNSNDV LREFIETGRY DLTHRSLKQT YSPAMDILKS SNVERVLFSL FGFERTLELM QALEEEKFYA LKPKELALLQ EHFSCASCSD EACLKTIQEV YAEHQYLIDP HTATALNASL KTNEKTLVSA TASYEKFPKT TLLALNEQKK DDKAALEALK NSYNTPDSQR LDDLFERGIK HQEVLKLNEI KSSILLWLES LH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctt ttgtccccac gcgctctttg aaagaaaaaa agattgattt tattgaagca atattaaacc ccaacgcccc caaaggcggg ctttacactt tagaaaattt tgaaacatta gaatggcaag attgtctgaa tctgagctat aacgagctag tggaatgcgt gtttgagcgg ttgggtttag agatttctaa gggtttattg acaagcgctt taaaacgcta tgaaaatttt gataacccta aaaatccagc ccctattttt gcgctcaatg aaaggctttt tgtccaagag ctctaccatg ggcctagttt ggcgtttaaa gacatggcgt tgcagccttt agcgagcttg ttttctaacc tagcggtagg aaaaaatgaa aagtatttaa tgctcgtttc cacgagtggc gatactggcc ctgcgacttt agaaagtttg gcgggcatgc ctaatgtttt tgtggtgtgt ttatacccca aagacggcac gagtttagtc caaaaactcc aaatggtaac ccaaagcgct aacaatttaa aagtctttgg gatcagtggg gattttgatg atgcgcaaaa cgcactcaaa aaccttttaa aagacgatga ttttaacgaa gccctaaaaa cgcaccaatt gaaattaagc gtggctaatt ccgtgaattt tgggcgcatc gcttttcaaa tcgtctatca tatttggggc tttttggaat tgtataaaaa aggcgcgatc aattctaaag aaaaaatcac cctagccata cctagcggta attttgggaa cgctttaggg gcgttttatg ccaaaaaaat gggcttgaat attgctaaaa tcaaggtggt aaccaacagc aatgatgttt taagggagtt tatagaaaca gggcgttacg atttaaccca tcgttcttta aagcaaactt actcgccggc tatggatatt ttaaaaagct ctaacgtgga aagggtgctt tttagccttt ttgggtttga acgcacacta gaattgatgc aagctttaga agaagagaaa ttttacgcct taaaacctaa agaattagcc cttttacagg agcatttttc ttgtgcgagc tgctcagatg aagcctgttt gaagacgatc caagaagttt atgcagagca ccaatacctg attgaccccc acaccgccac cgctttaaac gccagcttga aaaccaatga aaaaaccctt gtttctgcca ccgcctctta tgaaaaattc cctaaaacca cccttttagc cctaaacgag caaaaaaaag acgataaagc cgccctagaa gcactaaaaa acagctacaa taccccggat agccaacgcc ttgatgattt atttgaaaga ggaatcaagc accaagaagt tttaaaactc aatgagatca aatcttctat tcttttgtgg ctagaaagcc tacattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for HepyC.00545.a.B1.PW38241
PURIFICATION DATe: 4/4/2017
CONCENTRATION: 33.5mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP FVPTRSLKEK KIDFIEAILN PNAPKGGLYT LENFETLEWQ DCLNLSYNEL VECVFERLGL EISKGLLTSA LKRYENFDNP KNPAPIFALN ERLFVQELYH GPSLAFKDMA LQPLASLFSN LAVGKNEKYL MLVSTSGDTG PATLESLAGM PNVFVVCLYP KDGTSLVQKL QMVTQSANNL KVFGISGDFD DAQNALKNLL KDDDFNEALK THQLKLSVAN SVNFGRIAFQ IVYHIWGFLE LYKKGAINSK EKITLAIPSG NFGNALGAFY AKKMGLNIAK IKVVTNSNDV LREFIETGRY DLTHRSLKQT YSPAMDILKS SNVERVLFSL FGFERTLELM QALEEEKFYA LKPKELALLQ EHFSCASCSD EACLKTIQEV YAEHQYLIDP HTATALNASL KTNEKTLVSA TASYEKFPKT TLLALNEQKK DDKAALEALK NSYNTPDSQR LDDLFERGIK HQEVLKLNEI KSS
Validated NT Sequence
tagaagattt gatctcattg agttttaaaa cttcttggtg cttgattcct ctttcaaata aatcatcaag gcgttggcta tccggggtat tgtagctgtt ttttagtgct tctagggcgg ctttatcgtc ttttttttgc tcgtttaggg ctaaaagggt ggttttaggg aatttttcat aagaggcggt ggcagaaaca agggtttttt cattggtttt caagctggcg tttaaagcgg tggcggtgtg ggggtcaatc aggtattggt gctctgcata aacttcttgg atcgtcttca aacaggcttc atctgagcag ctcgcacaag aaaaatgctc ctgtaaaagg gctaattctt taggttttaa ggcgtaaaat ttctcttctt ctaaagcttg catcaattct agtgtgcgtt caaacccaaa aaggctaaaa agcacccttt ccacgttaga gctttttaaa atatccatag ccggcgagta agtttgcttt aaagaacgat gggttaaatc gtaacgccct gtttctataa actcccttaa aacatcattg ctgttggtta ccaccttgat tttagcaata ttcaagccca tttttttggc ataaaacgcc cctaaagcgt tcccaaaatt accgctaggt atggctaggg tgattttttc tttagaattg atcgcgcctt ttttatacaa ttccaaaaag ccccaaatat gatagacgat ttgaaaagcg atgcgcccaa aattcacgga attagccacg cttaatttca attggtgcgt ttttagggct tcgttaaaat catcgtcttt taaaaggttt ttgagtgcgt tttgcgcatc atcaaaatcc ccactgatcc caaagacttt taaattgtta gcgctttggg ttaccatttg gagtttttgg actaaactcg tgccgtcttt ggggtataaa cacaccacaa aaacattagg catgcccgcc aaactttcta aagtcgcagg gccagtatcg ccactcgtgg aaacgagcat taaatacttt tcattttttc ctaccgctag gttagaaaac aagctcgcta aaggctgcaa cgccatgtct ttaaacgcca aactaggccc atggtagagc tcttggacaa aaagcctttc attgagcgca aaaatagggg ctggattttt agggttatca aaattttcat agcgttttaa agcgcttgtc aataaaccct tagaaatctc taaacccaac cgctcaaaca cgcattccac tagctcgtta tagctcagat tcagacaatc ttgccattct aatgtttcaa aattttctaa agtgtaaagc ccgcctttgg gggcgttggg gtttaatatt gcttcaataa aatcaatctt tttttctttc aaagagcgcg tggggacaaa aggcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMP FVPTRSLKEK KIDFIEAILN PNAPKGGLYT LENFETLEWQ DCLNLSYNEL VECVFERLGL EISKGLLTSA LKRYENFDNP KNPAPIFALN ERLFVQELYH GPSLAFKDMA LQPLASLFSN LAVGKNEKYL MLVSTSGDTG PATLESLAGM PNVFVVCLYP KDGTSLVQKL QMVTQSANNL KVFGISGDFD DAQNALKNLL KDDDFNEALK THQLKLSVAN SVNFGRIAFQ IVYHIWGFLE LYKKGAINSK EKITLAIPSG NFGNALGAFY AKKMGLNIAK IKVVTNSNDV LREFIETGRY DLTHRSLKQT YSPAMDILKS SNVERVLFSL FGFERTLELM QALEEEKFYA LKPKELALLQ EHFSCASCSD EACLKTIQEV YAEHQYLIDP HTATALNASL KTNEKTLVSA TASYEKFPKT TLLALNEQKK DDKAALEALK NSYNTPDSQR LDDLFERGIK HQEVLKLNEI KSSILLWLES LH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTT TTGTCCCCAC GCGCTCTTTG AAAGAAAAAA AGATTGATTT TATTGAAGCA ATATTAAACC CCAACGCCCC CAAAGGCGGG CTTTACACTT TAGAAAATTT TGAAACATTA GAATGGCAAG ATTGTCTGAA TCTGAGCTAT AACGAGCTAG TGGAATGCGT GTTTGAGCGG TTGGGTTTAG AGATTTCTAA GGGTTTATTG ACAAGCGCTT TAAAACGCTA TGAAAATTTT GATAACCCTA AAAATCCAGC CCCTATTTTT GCGCTCAATG AAAGGCTTTT TGTCCAAGAG CTCTACCATG GGCCTAGTTT GGCGTTTAAA GACATGGCGT TGCAGCCTTT AGCGAGCTTG TTTTCTAACC TAGCGGTAGG AAAAAATGAA AAGTATTTAA TGCTCGTTTC CACGAGTGGC GATACTGGCC CTGCGACTTT AGAAAGTTTG GCGGGCATGC CTAATGTTTT TGTGGTGTGT TTATACCCCA AAGACGGCAC GAGTTTAGTC CAAAAACTCC AAATGGTAAC CCAAAGCGCT AACAATTTAA AAGTCTTTGG GATCAGTGGG GATTTTGATG ATGCGCAAAA CGCACTCAAA AACCTTTTAA AAGACGATGA TTTTAACGAA GCCCTAAAAA CGCACCAATT GAAATTAAGC GTGGCTAATT CCGTGAATTT TGGGCGCATC GCTTTTCAAA TCGTCTATCA TATTTGGGGC TTTTTGGAAT TGTATAAAAA AGGCGCGATC AATTCTAAAG AAAAAATCAC CCTAGCCATA CCTAGCGGTA ATTTTGGGAA CGCTTTAGGG GCGTTTTATG CCAAAAAAAT GGGCTTGAAT ATTGCTAAAA TCAAGGTGGT AACCAACAGC AATGATGTTT TAAGGGAGTT TATAGAAACA GGGCGTTACG ATTTAACCCA TCGTTCTTTA AAGCAAACTT ACTCGCCGGC TATGGATATT TTAAAAAGCT CTAACGTGGA AAGGGTGCTT TTTAGCCTTT TTGGGTTTGA ACGCACACTA GAATTGATGC AAGCTTTAGA AGAAGAGAAA TTTTACGCCT TAAAACCTAA AGAATTAGCC CTTTTACAGG AGCATTTTTC TTGTGCGAGC TGCTCAGATG AAGCCTGTTT GAAGACGATC CAAGAAGTTT ATGCAGAGCA CCAATACCTG ATTGACCCCC ACACCGCCAC CGCTTTAAAC GCCAGCTTGA AAACCAATGA AAAAACCCTT GTTTCTGCCA CCGCCTCTTA TGAAAAATTC CCTAAAACCA CCCTTTTAGC CCTAAACGAG CAAAAAAAAG ACGATAAAGC CGCCCTAGAA GCACTAAAAA ACAGCTACAA TACCCCGGAT AGCCAACGCC TTGATGATTT ATTTGAAAGA GGAATCAAGC ACCAAGAAGT TTTAAAACTC AATGAGATCA AATCTTCTAT TCTTTTGTGG CTAGAAAGCC TACATTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT