HepyC.00507.a

Ketol-acid reductoisomerase (EC 1.1.1.86) (Acetohydroxy-acid isomeroreductase) (Alpha-keto-beta-hydroxylacyl reductoisomerase)

CENTER ID: HepyC.00507.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00507.a.B1.GE40935 full length 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.346
UniProt: B5ZA98

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKTLGDLIL ALPVYYDKDI DLGVIQSLQV GIIGYGVQGE AQALNLRDSK VRVRIGLYQG SLSVSKAKKE GFEVLGVKEL VQQSDVIMAL LPDELHKEVL EKEVIPFLKE GQIVGFAHGF SVHFNQVVLP KGVGAILVAP KGPGSALREE YLKNKGLYHL IAIEQENSKN NAKAVALSYA KAMGGGRMGV LETSFKEECE SDLFGEQAVL CGGLEAIVRM GFETLIKAGY PEELAYFECV HEVKLVADLL HYKGVEGLRK HISNTAEFGA IKAREPMAKL LKKRMQKILK KIQNGSFAKD FLLEKSLNYP RLNTERKALK ETKIEQIGEI LRVPFNRKK
NT Sequence
ttgcttaaaa cgctaggaga tttgattttg gcattacccg tttattatga taaagacatt gatttaggtg ttatccaatc cttacaagtg ggcattattg gctatggcgt gcaaggagaa gcccaagcgc tcaatttgag ggactctaaa gtaagggtgc gtattggctt gtatcaaggg agtttgagcg tttcaaaagc aaaaaaagag ggctttgagg tgctaggagt caaggaatta gtccaacaat cggatgtgat catggcactt cttccggacg aattgcataa ggaagtgtta gaaaaagaag tgatcccttt tttaaaagaa gggcaaattg tgggctttgc ccatggtttt agcgtgcatt ttaatcaggt tgttctccca aaaggcgtgg gcgcgatttt agttgcgcca aaagggcctg ggagcgcttt aagagaagaa taccttaaaa ataagggctt ataccattta atcgccatag agcaagaaaa ttcaaaaaat aacgctaaag cggtggcttt aagctacgcc aaagcgatgg gtggggggag aatgggggtt ttagaaacga gctttaaaga agaatgcgag agcgatttat tcggcgagca agcggttttg tgtggggggt tagaagcgat tgtaagaatg gggtttgaaa ccttgatcaa ggcaggatac cctgaagaat tagcctattt tgaatgcgtg catgaagtga aattggtggc ggatttatta cattataagg gggtagaggg tttaaggaaa cacatttcta acaccgctga atttggggcg attaaagcta gagagcctat ggcaaagctg ctaaaaaaac gcatgcaaaa aatcttaaaa aagattcaaa acggctcatt cgctaaggat tttttactag aaaagagctt gaattacccc aggttaaaca cagagaggaa agcccttaaa gagactaaaa tagaacaaat tggggaaatt ttacgcgtcc cattcaatcg taaaaaataa
Details for HepyC.00507.a.B1.GE40935
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML LKTLGDLILA LPVYYDKDID LGVIQSLQVG IIGYGVQGEA QALNLRDSKV RVRIGLYQGS LSVSKAKKEG FEVLGVKELV QQSDVIMALL PDELHKEVLE KEVIPFLKEG QIVGFAHGFS VHFNQVVLPK GVGAILVAPK GPGSALREEY LKNKGLYHLI AIEQENSKNN AKAVALSYAK AMGGGRMGVL ETSFKEECES DLFGEQAVLC GGLEAIVRMG FETLIKAGYP EELAYFECVH EVKLVADLLH YKGVEGLRKH ISNTAEFGAI KAREPMAKLL KKRMQKILKK IQNGSFAKDF LLEKSLNYPR LNTERKALKE TKIEQIGEI
Validated NT Sequence
aaaatttccc caatttgttc tattttagtc tctttaaggg ctttcctctc tgtgtttaac ctggggtaat tcaagctctt ttctagtaaa aaatccttag cgaatgagcc gttttgaatc ttttttaaga ttttttgcat gcgttttttt agcagctttg ccataggctc tctagcttta atcgccccaa attcagcggt gttagaaatg tgtttcctta aaccctctac ccccttataa tgtaataaat ccgccaccaa tttcacttca tgcacgcatt caaaataggc taattcttca gggtatcctg ccttgatcaa ggtttcaaac cccattctta caatcgcttc taacccccca cacaaaaccg cttgctcgcc gaataaatcg ctctcgcatt cttctttaaa gctcgtttct aaaaccccca ttctcccccc acccatcgct ttggcgtagc ttaaagccac cgctttagcg ttattttttg aattttcttg ctctatggcg attaaatggt ataagccctt atttttaagg tattcttctc ttaaagcgct cccaggccct tttggcgcaa ctaaaatcgc gcccacgcct tttgggagaa caacctgatt aaaatgcacg ctaaaaccat gggcaaagcc cacaatttgc ccttctttta aaaaagggat cacttctttt tctaacactt ccttatgcaa ttcgtccgga agaagtgcca tgatcacatc cgattgttgg actaattcct tgactcctag cacctcaaag ccctcttttt ttgcttttga aacgctcaaa ctcccttgat acaagccaat acgcaccctt actttagagt ccctcaaatt gagcgcttgg gcttctcctt gcacgccata gccaataatg cccacttgta aggattggat aacacctaaa tcaatgtctt tatcataata aacgggtaat gccaaaatca aatctcctag cgttttaagc aacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHML KTLGDLILAL PVYYDKDIDL GVIQSLQVGI IGYGVQGEAQ ALNLRDSKVR VRIGLYQGSL SVSKAKKEGF EVLGVKELVQ QSDVIMALLP DELHKEVLEK EVIPFLKEGQ IVGFAHGFSV HFNQVVLPKG VGAILVAPKG PGSALREEYL KNKGLYHLIA IEQENSKNNA KAVALSYAKA MGGGRMGVLE TSFKEECESD LFGEQAVLCG GLEAIVRMGF ETLIKAGYPE ELAYFECVHE VKLVADLLHY KGVEGLRKHI SNTAEFGAIK AREPMAKLLK KRMQKILKKI QNGSFAKDFL LEKSLNYPRL NTERKALKET KIEQIGEILR VPFNRKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgctta aaacgctagg agatttgatt ttggcattac ccgtttatta tgataaagac attgatttag gtgttatcca atccttacaa gtgggcatta ttggctatgg cgtgcaagga gaagcccaag cgctcaattt gagggactct aaagtaaggg tgcgtattgg cttgtatcaa gggagtttga gcgtttcaaa agcaaaaaaa gagggctttg aggtgctagg agtcaaggaa ttagtccaac aatcggatgt gatcatggca cttcttccgg acgaattgca taaggaagtg ttagaaaaag aagtgatccc ttttttaaaa gaagggcaaa ttgtgggctt tgcccatggt tttagcgtgc attttaatca ggttgttctc ccaaaaggcg tgggcgcgat tttagttgcg ccaaaagggc ctgggagcgc tttaagagaa gaatacctta aaaataaggg cttataccat ttaatcgcca tagagcaaga aaattcaaaa aataacgcta aagcggtggc tttaagctac gccaaagcga tgggtggggg gagaatgggg gttttagaaa cgagctttaa agaagaatgc gagagcgatt tattcggcga gcaagcggtt ttgtgtgggg ggttagaagc gattgtaaga atggggtttg aaaccttgat caaggcagga taccctgaag aattagccta ttttgaatgc gtgcatgaag tgaaattggt ggcggattta ttacattata agggggtaga gggtttaagg aaacacattt ctaacaccgc tgaatttggg gcgattaaag ctagagagcc tatggcaaag ctgctaaaaa aacgcatgca aaaaatctta aaaaagattc aaaacggctc attcgctaag gattttttac tagaaaagag cttgaattac cccaggttaa acacagagag gaaagccctt aaagagacta aaatagaaca aattggggaa attttacgcg tcccattcaa tcgtaaaaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat