HepyC.00487.a
Dihydroorotate dehydrogenase (quinone) (EC 1.3.5.2) (DHOdehase) (DHOD) (DHODase) (Dihydroorotate oxidase)
| CENTER ID: | HepyC.00487.a |
| ORGANISM: | Helicobacter pylori G27 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.00487.a.B1.GE40934 | full length | 1 | 351 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| HepyC.00487.a.B1.PW38288 | full length | 1 | 351 | ||
| HepyC.00487.a.B1.PW38289 | full length | 1 | 351 |
Structures
6B8S
DEPOSITED: 10/9/2017
DETERMINATION: XRay
CLONE: HepyC.00487.a.B1.GE40934
PROTEIN: HepyC.00487.a.B1.PW38289
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|563041.6.peg.453 |
| UniProt: | B5Z6I2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLYPLVKKYL FSLDAEDAHE KVCKILRTLS KSSFLCSLIH SQWGYKNPKL ENEILGLNFP NPLGLAAGFD KNASMLRALI AFGFGYLEAG TLTNEAQVGN ERPRLFRHIE EESLQNAMGF NNYGAVLGAR SFNRFAPYKT PIGINLGKNK HIEQAHALED YKAVLNQCLN IGDYYTFNLS SPNTPNLRDL QNKAFVNELF CMAKEMTHKP LFLKIAPDLE IDDMLEIVNS AIEAGAHGII ATNTTIDKSL VFAPKEMGGL SGKCLTKKSR EVFKELAKAF FNKSVLVSVG GISDAKEAYE RIKMGASLLQ IYSAFIYNGP NLCQNILKDL VKLLQKDGFL SVKEAIGADL R
NT Sequence
atgctttatc cattagtaaa aaaatatctt tttagcctag acgctgaaga cgcgcacgaa aaagtttgta agattttaag aacgctttct aaatcgagct ttttgtgtag tttaatccat tctcaatggg gttataaaaa cccaaagctt gaaaatgaaa ttttaggctt aaatttccct aaccctttag gcttagccgc cggttttgat aaaaacgctt ccatgctcag ggcgttaatt gcttttgggt ttggctattt agaagcaggc actctcacca atgaagcgca agtggggaat gaaagaccca ggcttttcag gcacattgaa gaagagtcct tacaaaatgc gatggggttt aataattacg gagcggtttt aggagcgaga tcgttcaatc gcttcgcccc ttataaaacc cctattggca tcaatttagg caaaaacaaa cacatagagc aagcgcatgc cctagaagat tacaaggcgg ttttaaatca atgtttaaac attggcgatt attacacttt caacctttct tcgcccaaca cccctaattt aagggattta caaaacaaag cgtttgtgaa tgagcttttt tgcatggcga aagaaatgac ccataagcct ttatttttaa aaatcgcccc ggatttagaa atagatgaca tgctagagat tgtcaatagc gctattgaag ccggagcgca tgggattatt gcgactaata cgaccattga taaaagcctg gtattcgctc ctaaagaaat ggggggcttg agcgggaaat gcttgactaa aaaaagccgt gaagtcttta aagaactggc taaagctttt tttaataaaa gcgttcttgt ttctgtgggg gggattagcg atgccaaaga agcttatgaa aggattaaaa tgggagcgag tctgttacaa atttatagtg cttttattta caacgggcca aatttatgcc aaaatattct taaagatttg gtaaaattac tccaaaaaga tggatttttg agcgttaaag aggctatagg agcggattta agatga
Details for HepyC.00487.a.B1.GE40934
| HARVESTED ON: | 12/20/2016 |
| SEQUENCED ON: | 12/27/2016 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHML YPLVKKYLFS LDAEDAHEKV CKILRTLSKS SFLCSLIHSQ WGYKNPKLEN EILGLNFPNP LGLAAGFDKN ASMLRALIAF GFGYLEAGTL TNEAQVGNER PRLFRHIEEE SLQNAMGFNN YGAVLGARSF NRFAPYKTPI GINLGKNKHI EQAHALEDYK AVLNQCLNIG DYYTFNLSSP NTPNLRDLQN KAFVNELFCM AKEMTHKPLF LKIAPDLEID DMLEIVNSAI EAGAHGIIAT NTTIDKSLVF APKEMGGLSG KCLTKKSREV FKELAKAFFN KSVLVSVGGI SDAKEAYERI KMGASLLQIY SAFIYNGPNL CQNILKDLVK LLQKDGFLSV K
Validated NT Sequence
ctttaacgct caaaaatcca tctttttgga gtaattttac caaatcttta agaatatttt ggcataaatt tggcccgttg taaataaaag cactataaat ttgtaacaga ctcgctccca ttttaatcct ttcataagct tctttggcat cgctaatccc ccccacagaa acaagaacgc ttttattaaa aaaagcttta gccagttctt taaagacttc acggcttttt ttagtcaagc atttcccgct caagcccccc atttctttag gagcgaatac caggctttta tcaatggtcg tattagtcgc aataatccca tgcgctccgg cttcaatagc gctattgaca atctctagca tgtcatctat ttctaaatcc ggggcgattt ttaaaaataa aggcttatgg gtcatttctt tcgccatgca aaaaagctca ttcacaaacg ctttgttttg taaatccctt aaattagggg tgttgggcga agaaaggttg aaagtgtaat aatcgccaat gtttaaacat tgatttaaaa ccgccttgta atcttctagg gcatgcgctt gctctatgtg tttgtttttg cctaaattga tgccaatagg ggttttataa ggggcgaagc gattgaacga tctcgctcct aaaaccgctc cgtaattatt aaaccccatc gcattttgta aggactcttc ttcaatgtgc ctgaaaagcc tgggtctttc attccccact tgcgcttcat tggtgagagt gcctgcttct aaatagccaa acccaaaagc aattaacgcc ctgagcatgg aagcgttttt atcaaaaccg gcggctaagc ctaaagggtt agggaaattt aagcctaaaa tttcattttc aagctttggg tttttataac cccattgaga atggattaaa ctacacaaaa agctcgattt agaaagcgtt cttaaaatct tacaaacttt ttcgtgcgcg tcttcagcgt ctaggctaaa aagatatttt tttactaatg gataaagcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHML YPLVKKYLFS LDAEDAHEKV CKILRTLSKS SFLCSLIHSQ WGYKNPKLEN EILGLNFPNP LGLAAGFDKN ASMLRALIAF GFGYLEAGTL TNEAQVGNER PRLFRHIEEE SLQNAMGFNN YGAVLGARSF NRFAPYKTPI GINLGKNKHI EQAHALEDYK AVLNQCLNIG DYYTFNLSSP NTPNLRDLQN KAFVNELFCM AKEMTHKPLF LKIAPDLEID DMLEIVNSAI EAGAHGIIAT NTTIDKSLVF APKEMGGLSG KCLTKKSREV FKELAKAFFN KSVLVSVGGI SDAKEAYERI KMGASLLQIY SAFIYNGPNL CQNILKDLVK LLQKDGFLSV KEAIGADLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttt atccattagt aaaaaaatat ctttttagcc tagacgctga agacgcgcac gaaaaagttt gtaagatttt aagaacgctt tctaaatcga gctttttgtg tagtttaatc cattctcaat ggggttataa aaacccaaag cttgaaaatg aaattttagg cttaaatttc cctaaccctt taggcttagc cgccggtttt gataaaaacg cttccatgct cagggcgtta attgcttttg ggtttggcta tttagaagca ggcactctca ccaatgaagc gcaagtgggg aatgaaagac ccaggctttt caggcacatt gaagaagagt ccttacaaaa tgcgatgggg tttaataatt acggagcggt tttaggagcg agatcgttca atcgcttcgc cccttataaa acccctattg gcatcaattt aggcaaaaac aaacacatag agcaagcgca tgccctagaa gattacaagg cggttttaaa tcaatgttta aacattggcg attattacac tttcaacctt tcttcgccca acacccctaa tttaagggat ttacaaaaca aagcgtttgt gaatgagctt ttttgcatgg cgaaagaaat gacccataag cctttatttt taaaaatcgc cccggattta gaaatagatg acatgctaga gattgtcaat agcgctattg aagccggagc gcatgggatt attgcgacta atacgaccat tgataaaagc ctggtattcg ctcctaaaga aatggggggc ttgagcggga aatgcttgac taaaaaaagc cgtgaagtct ttaaagaact ggctaaagct ttttttaata aaagcgttct tgtttctgtg ggggggatta gcgatgccaa agaagcttat gaaaggatta aaatgggagc gagtctgtta caaatttata gtgcttttat ttacaacggg ccaaatttat gccaaaatat tcttaaagat ttggtaaaat tactccaaaa agatggattt ttgagcgtta aagaggctat aggagcggat ttaagatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for HepyC.00487.a.B1.PW38288
| PURIFICATION DATe: | 5/30/2017 |
| CONCENTRATION: | 31mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML YPLVKKYLFS LDAEDAHEKV CKILRTLSKS SFLCSLIHSQ WGYKNPKLEN EILGLNFPNP LGLAAGFDKN ASMLRALIAF GFGYLEAGTL TNEAQVGNER PRLFRHIEEE SLQNAMGFNN YGAVLGARSF NRFAPYKTPI GINLGKNKHI EQAHALEDYK AVLNQCLNIG DYYTFNLSSP NTPNLRDLQN KAFVNELFCM AKEMTHKPLF LKIAPDLEID DMLEIVNSAI EAGAHGIIAT NTTIDKSLVF APKEMGGLSG KCLTKKSREV FKELAKAFFN KSVLVSVGGI SDAKEAYERI KMGASLLQIY SAFIYNGPNL CQNILKDLVK LLQKDGFLSV K
Validated NT Sequence
ctttaacgct caaaaatcca tctttttgga gtaattttac caaatcttta agaatatttt ggcataaatt tggcccgttg taaataaaag cactataaat ttgtaacaga ctcgctccca ttttaatcct ttcataagct tctttggcat cgctaatccc ccccacagaa acaagaacgc ttttattaaa aaaagcttta gccagttctt taaagacttc acggcttttt ttagtcaagc atttcccgct caagcccccc atttctttag gagcgaatac caggctttta tcaatggtcg tattagtcgc aataatccca tgcgctccgg cttcaatagc gctattgaca atctctagca tgtcatctat ttctaaatcc ggggcgattt ttaaaaataa aggcttatgg gtcatttctt tcgccatgca aaaaagctca ttcacaaacg ctttgttttg taaatccctt aaattagggg tgttgggcga agaaaggttg aaagtgtaat aatcgccaat gtttaaacat tgatttaaaa ccgccttgta atcttctagg gcatgcgctt gctctatgtg tttgtttttg cctaaattga tgccaatagg ggttttataa ggggcgaagc gattgaacga tctcgctcct aaaaccgctc cgtaattatt aaaccccatc gcattttgta aggactcttc ttcaatgtgc ctgaaaagcc tgggtctttc attccccact tgcgcttcat tggtgagagt gcctgcttct aaatagccaa acccaaaagc aattaacgcc ctgagcatgg aagcgttttt atcaaaaccg gcggctaagc ctaaagggtt agggaaattt aagcctaaaa tttcattttc aagctttggg tttttataac cccattgaga atggattaaa ctacacaaaa agctcgattt agaaagcgtt cttaaaatct tacaaacttt ttcgtgcgcg tcttcagcgt ctaggctaaa aagatatttt tttactaatg gataaagcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHML YPLVKKYLFS LDAEDAHEKV CKILRTLSKS SFLCSLIHSQ WGYKNPKLEN EILGLNFPNP LGLAAGFDKN ASMLRALIAF GFGYLEAGTL TNEAQVGNER PRLFRHIEEE SLQNAMGFNN YGAVLGARSF NRFAPYKTPI GINLGKNKHI EQAHALEDYK AVLNQCLNIG DYYTFNLSSP NTPNLRDLQN KAFVNELFCM AKEMTHKPLF LKIAPDLEID DMLEIVNSAI EAGAHGIIAT NTTIDKSLVF APKEMGGLSG KCLTKKSREV FKELAKAFFN KSVLVSVGGI SDAKEAYERI KMGASLLQIY SAFIYNGPNL CQNILKDLVK LLQKDGFLSV KEAIGADLR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTTT ATCCATTAGT AAAAAAATAT CTTTTTAGCC TAGACGCTGA AGACGCGCAC GAAAAAGTTT GTAAGATTTT AAGAACGCTT TCTAAATCGA GCTTTTTGTG TAGTTTAATC CATTCTCAAT GGGGTTATAA AAACCCAAAG CTTGAAAATG AAATTTTAGG CTTAAATTTC CCTAACCCTT TAGGCTTAGC CGCCGGTTTT GATAAAAACG CTTCCATGCT CAGGGCGTTA ATTGCTTTTG GGTTTGGCTA TTTAGAAGCA GGCACTCTCA CCAATGAAGC GCAAGTGGGG AATGAAAGAC CCAGGCTTTT CAGGCACATT GAAGAAGAGT CCTTACAAAA TGCGATGGGG TTTAATAATT ACGGAGCGGT TTTAGGAGCG AGATCGTTCA ATCGCTTCGC CCCTTATAAA ACCCCTATTG GCATCAATTT AGGCAAAAAC AAACACATAG AGCAAGCGCA TGCCCTAGAA GATTACAAGG CGGTTTTAAA TCAATGTTTA AACATTGGCG ATTATTACAC TTTCAACCTT TCTTCGCCCA ACACCCCTAA TTTAAGGGAT TTACAAAACA AAGCGTTTGT GAATGAGCTT TTTTGCATGG CGAAAGAAAT GACCCATAAG CCTTTATTTT TAAAAATCGC CCCGGATTTA GAAATAGATG ACATGCTAGA GATTGTCAAT AGCGCTATTG AAGCCGGAGC GCATGGGATT ATTGCGACTA ATACGACCAT TGATAAAAGC CTGGTATTCG CTCCTAAAGA AATGGGGGGC TTGAGCGGGA AATGCTTGAC TAAAAAAAGC CGTGAAGTCT TTAAAGAACT GGCTAAAGCT TTTTTTAATA AAAGCGTTCT TGTTTCTGTG GGGGGGATTA GCGATGCCAA AGAAGCTTAT GAAAGGATTA AAATGGGAGC GAGTCTGTTA CAAATTTATA GTGCTTTTAT TTACAACGGG CCAAATTTAT GCCAAAATAT TCTTAAAGAT TTGGTAAAAT TACTCCAAAA AGATGGATTT TTGAGCGTTA AAGAGGCTAT AGGAGCGGAT TTAAGATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for HepyC.00487.a.B1.PW38289
| PURIFICATION DATe: | 5/30/2017 |
| CONCENTRATION: | 18.5mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML YPLVKKYLFS LDAEDAHEKV CKILRTLSKS SFLCSLIHSQ WGYKNPKLEN EILGLNFPNP LGLAAGFDKN ASMLRALIAF GFGYLEAGTL TNEAQVGNER PRLFRHIEEE SLQNAMGFNN YGAVLGARSF NRFAPYKTPI GINLGKNKHI EQAHALEDYK AVLNQCLNIG DYYTFNLSSP NTPNLRDLQN KAFVNELFCM AKEMTHKPLF LKIAPDLEID DMLEIVNSAI EAGAHGIIAT NTTIDKSLVF APKEMGGLSG KCLTKKSREV FKELAKAFFN KSVLVSVGGI SDAKEAYERI KMGASLLQIY SAFIYNGPNL CQNILKDLVK LLQKDGFLSV K
Validated NT Sequence
ctttaacgct caaaaatcca tctttttgga gtaattttac caaatcttta agaatatttt ggcataaatt tggcccgttg taaataaaag cactataaat ttgtaacaga ctcgctccca ttttaatcct ttcataagct tctttggcat cgctaatccc ccccacagaa acaagaacgc ttttattaaa aaaagcttta gccagttctt taaagacttc acggcttttt ttagtcaagc atttcccgct caagcccccc atttctttag gagcgaatac caggctttta tcaatggtcg tattagtcgc aataatccca tgcgctccgg cttcaatagc gctattgaca atctctagca tgtcatctat ttctaaatcc ggggcgattt ttaaaaataa aggcttatgg gtcatttctt tcgccatgca aaaaagctca ttcacaaacg ctttgttttg taaatccctt aaattagggg tgttgggcga agaaaggttg aaagtgtaat aatcgccaat gtttaaacat tgatttaaaa ccgccttgta atcttctagg gcatgcgctt gctctatgtg tttgtttttg cctaaattga tgccaatagg ggttttataa ggggcgaagc gattgaacga tctcgctcct aaaaccgctc cgtaattatt aaaccccatc gcattttgta aggactcttc ttcaatgtgc ctgaaaagcc tgggtctttc attccccact tgcgcttcat tggtgagagt gcctgcttct aaatagccaa acccaaaagc aattaacgcc ctgagcatgg aagcgttttt atcaaaaccg gcggctaagc ctaaagggtt agggaaattt aagcctaaaa tttcattttc aagctttggg tttttataac cccattgaga atggattaaa ctacacaaaa agctcgattt agaaagcgtt cttaaaatct tacaaacttt ttcgtgcgcg tcttcagcgt ctaggctaaa aagatatttt tttactaatg gataaagcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHML YPLVKKYLFS LDAEDAHEKV CKILRTLSKS SFLCSLIHSQ WGYKNPKLEN EILGLNFPNP LGLAAGFDKN ASMLRALIAF GFGYLEAGTL TNEAQVGNER PRLFRHIEEE SLQNAMGFNN YGAVLGARSF NRFAPYKTPI GINLGKNKHI EQAHALEDYK AVLNQCLNIG DYYTFNLSSP NTPNLRDLQN KAFVNELFCM AKEMTHKPLF LKIAPDLEID DMLEIVNSAI EAGAHGIIAT NTTIDKSLVF APKEMGGLSG KCLTKKSREV FKELAKAFFN KSVLVSVGGI SDAKEAYERI KMGASLLQIY SAFIYNGPNL CQNILKDLVK LLQKDGFLSV KEAIGADLR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTTT ATCCATTAGT AAAAAAATAT CTTTTTAGCC TAGACGCTGA AGACGCGCAC GAAAAAGTTT GTAAGATTTT AAGAACGCTT TCTAAATCGA GCTTTTTGTG TAGTTTAATC CATTCTCAAT GGGGTTATAA AAACCCAAAG CTTGAAAATG AAATTTTAGG CTTAAATTTC CCTAACCCTT TAGGCTTAGC CGCCGGTTTT GATAAAAACG CTTCCATGCT CAGGGCGTTA ATTGCTTTTG GGTTTGGCTA TTTAGAAGCA GGCACTCTCA CCAATGAAGC GCAAGTGGGG AATGAAAGAC CCAGGCTTTT CAGGCACATT GAAGAAGAGT CCTTACAAAA TGCGATGGGG TTTAATAATT ACGGAGCGGT TTTAGGAGCG AGATCGTTCA ATCGCTTCGC CCCTTATAAA ACCCCTATTG GCATCAATTT AGGCAAAAAC AAACACATAG AGCAAGCGCA TGCCCTAGAA GATTACAAGG CGGTTTTAAA TCAATGTTTA AACATTGGCG ATTATTACAC TTTCAACCTT TCTTCGCCCA ACACCCCTAA TTTAAGGGAT TTACAAAACA AAGCGTTTGT GAATGAGCTT TTTTGCATGG CGAAAGAAAT GACCCATAAG CCTTTATTTT TAAAAATCGC CCCGGATTTA GAAATAGATG ACATGCTAGA GATTGTCAAT AGCGCTATTG AAGCCGGAGC GCATGGGATT ATTGCGACTA ATACGACCAT TGATAAAAGC CTGGTATTCG CTCCTAAAGA AATGGGGGGC TTGAGCGGGA AATGCTTGAC TAAAAAAAGC CGTGAAGTCT TTAAAGAACT GGCTAAAGCT TTTTTTAATA AAAGCGTTCT TGTTTCTGTG GGGGGGATTA GCGATGCCAA AGAAGCTTAT GAAAGGATTA AAATGGGAGC GAGTCTGTTA CAAATTTATA GTGCTTTTAT TTACAACGGG CCAAATTTAT GCCAAAATAT TCTTAAAGAT TTGGTAAAAT TACTCCAAAA AGATGGATTT TTGAGCGTTA AAGAGGCTAT AGGAGCGGAT TTAAGATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T