SSGCID: Target Page

HepyC.00168.a

Bifunctional enzyme IspD/IspF [Includes: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) (4-diphosphocytidyl-2C-methyl-D-erythritol synthase) (MEP cytidylyltransferase) (MCT); 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MECDP-synthase) (MECPP-synthase) (MECPS) (EC 4.6.1.12)]

CENTER ID:	HepyC.00168.a
ORGANISM:	Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
HepyC.00168.a.B1.GE40851	full length		1	406

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|563041.6.peg.444
UniProt:	B5Z6H4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLIRVNGEA FKLSLESLEE DPFETKETLE TLVKQTSVVL LAAGESRRFS QTIKKQWLRS NHTPLWLSVY ESFKKALDFK EILLVVSELD YIYIKRHYPE IKLVKGGASR QESVRNALKI IDSAYTLTSD AARGLANIEA LKSLFLTLQQ TNHYCIAPYL PCYDTAIYYN EALDREAIKL IQTPQLSHTK TLQSALNQGD FKDESSAILQ AFPNRVSYIE GSKNLHKLTT SDDLKHFAFF FNPAKDTFIG MGFDTHAFIK DKPMVLGGVV LDCEFGLKAH SDGDALLHAV IDAVLGAIKG GDIGEWFPDN DPKYKNASSK ELLKVVLDFS QSIGFELLEM GATIFSEIPK ITPYKPAILE SLSQLLGLEK SQISLKATTM EKMGFIGKQE GLLVQAHVSM RYKQKL

NT Sequence

atgtctttga ttagagtgaa tggggaagct tttaaactct ctttagaaag tttagaagaa gacccttttg aaactaaaga aacgctagaa acgcttgtca aacaaacgag cgttgtttta ttggctgctg gggaatctag gcgtttttct caaaccatca aaaaacaatg gctacgctct aatcataccc ccttatggct cagcgtttat gaaagcttta aaaaagccct agactttaaa gaaatccttt tagtcgtaag cgaattggat tatatttaca tcaaacgcca ttaccctgaa atcaagcttg taaaaggcgg ggcatcaagg caagaatccg tgcgtaacgc tttaaaaata attgatagcg cttacacgct caccagcgat gcggctaggg gtttagccaa tattgaagca ctcaaaagct tgtttttgac cctccaacaa acgaaccatt attgcatcgc tccttacttg ccttgctatg acacagcgat ctattataac gaggctttag atagagaggc gatcaaactc attcaaaccc cgcaattaag ccacactaaa acgctccaat cagccctaaa ccaaggggat tttaaagatg aaagcagcgc gattttacaa gctttcccta atcgtgtgag ctatattgaa ggcagtaaaa atttgcacaa gctcaccacg agcgatgatt tgaagcattt tgcgttcttt ttcaacccag caaaagacac ttttatcggc atgggctttg atacgcatgc gttcattaaa gataagccta tggttttagg gggggttgtt ttggattgcg agtttgggtt aaaggctcat agcgacggcg atgctttgtt gcatgcggtt attgatgcgg ttttaggagc gattaaaggg ggggatattg gcgaatggtt ccctgataat gatcccaaat acaaaaacgc ctcttctaaa gagcttttaa aagtcgtgtt ggatttttct caaagcattg ggtttgaatt gcttgaaatg ggagcgacca tctttagcga aatccctaaa atcactcctt acaaaccggc gattttagag agcttgagcc aacttttggg tttagaaaaa tctcaaatca gcttaaaagc caccacaatg gaaaaaatgg ggttcattgg caaacaagaa gggctgttag tccaagcgca tgtgagcatg cgttataaac aaaaacttta a

Details for HepyC.00168.a.B1.GE40851

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS LIRVNGEAFK LSLESLEEDP FETKETLETL VKQTSVVLLA AGESRRFSQT IKKQWLRSNH TPLWLSVYES FKKALDFKEI LLVVSELDYI YIKRHYPEIK LVKGGASRQE SVRNALKIID SAYTLTSDAA RGLANIEALK SLFLTLQQTN HYCIAPYLPC YDTAIYYNEA LDREAIKLIQ TPQLSHTKTL QSALNQGDFK DESSAILQAF PNRVSYIEGS KNLHKLTTSD DLKHFAFFFN PAKDTFIGMG FDTHAFIKDK PMVLGGVVLD CEFGLKAHSD GDALLHAVID AVLGAIKGGD IGEWFPDNDP KYKNASSKEL LKVVLDFSQS IGFELLEMGA TIFSEIPKIT PYKPAILESL SQLLGLGKSQ ISLKATTMEK MGFIGKQEGL LVQAHV

Validated NT Sequence

gntcacatgc gcttggacta acagcccttc ttgtttgcca atgaacccca ttttttccat tgtggtggct tttaagctga tttgagattt tcctaaaccc aaaagttggc tcaagctctc taaaatcgcc ggtttgtaag gagtgatttt agggatttcg ctaaagatgg tcgctcccat ttcaagcaat tcaaacccaa tgctttgaga aaaatccaac acgactttta aaagctcttt agaagaggcg tttttgtatt tgggatcatt atcagggaac cattcgccaa tatccccccc tttaatcgct cctaaaaccg catcaataac cgcatgcaac aaagcatcgc cgtcgctatg agcctttaac ccaaactcgc aatccaaaac aaccccccct aaaaccatag gcttatcttt aatgaacgca tgcgtatcaa agcccatgcc gataaaagtg tcttttgctg ggttgaaaaa gaacgcaaaa tgcttcaaat catcgctcgt ggtgagcttg tgcaaatttt tactgccttc aatatagctc acacgattag ggaaagcttg taaaatcgcg ctgctttcat ctttaaaatc cccttggttt agggctgatt ggagcgtttt agtgtggctt aattgcgggg tttgaatgag tttgatcgcc tctctatcta aagcctcgtt ataatagatc gctgtgtcat agcaaggcaa gtaaggagcg atgcaataat ggttcgtttg ttggagggtc aaaaacaagc ttttgagtgc ttcaatattg gctaaacccc tagccgcatc gctggtgagc gtgtaagcgc tatcaattat ttttaaagcg ttacgcacgg attcttgcct tgatgccccg ccttttacaa gcttgatttc agggtaatgg cgtttgatgt aaatataatc caattcgctt acgactaaaa ggatttcttt aaagtctagg gcttttttaa agctttcata aacgctgagc cataaggggg tatgattaga gcgtagccat tgttttttga tggtttgaga aaaacgccta gattccccag cagccaataa aacaacgctc gtttgtttga caagcgtttc tagcgtttct ttagtttcaa aagggtcttc ttctaaactt tctaaagaga gtttaaaagc ttccccattc actctaatca aagacatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt tgattagagt gaatggggaa gcttttaaac tctctttaga aagtttagaa gaagaccctt ttgaaactaa agaaacgcta gaaacgcttg tcaaacaaac gagcgttgtt ttattggctg ctggggaatc taggcgtttt tctcaaacca tcaaaaaaca atggctacgc tctaatcata cccccttatg gctcagcgtt tatgaaagct ttaaaaaagc cctagacttt aaagaaatcc ttttagtcgt aagcgaattg gattatattt acatcaaacg ccattaccct gaaatcaagc ttgtaaaagg cggggcatca aggcaagaat ccgtgcgtaa cgctttaaaa ataattgata gcgcttacac gctcaccagc gatgcggcta ggggtttagc caatattgaa gcactcaaaa gcttgttttt gaccctccaa caaacgaacc attattgcat cgctccttac ttgccttgct atgacacagc gatctattat aacgaggctt tagatagaga ggcgatcaaa ctcattcaaa ccccgcaatt aagccacact aaaacgctcc aatcagccct aaaccaaggg gattttaaag atgaaagcag cgcgatttta caagctttcc ctaatcgtgt gagctatatt gaaggcagta aaaatttgca caagctcacc acgagcgatg atttgaagca ttttgcgttc tttttcaacc cagcaaaaga cacttttatc ggcatgggct ttgatacgca tgcgttcatt aaagataagc ctatggtttt agggggggtt gttttggatt gcgagtttgg gttaaaggct catagcgacg gcgatgcttt gttgcatgcg gttattgatg cggttttagg agcgattaaa gggggggata ttggcgaatg gttccctgat aatgatccca aatacaaaaa cgcctcttct aaagagcttt taaaagtcgt gttggatttt tctcaaagca ttgggtttga attgcttgaa atgggagcga ccatctttag cgaaatccct aaaatcactc cttacaaacc ggcgatttta gagagcttga gccaactttt gggtttagaa aaatctcaaa tcagcttaaa agccaccaca atggaaaaaa tggggttcat tggcaaacaa gaagggctgt tagtccaagc gcatgtgagc atgcgttata aacaaaaact ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat