HepyC.00133.a

Cysteine--tRNA ligase (EC 6.1.1.16) (Cysteinyl-tRNA synthetase) (CysRS)

CENTER ID: HepyC.00133.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00133.a.B1.GE40848 full length 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00133.a.B1.PS38169 full length 1 465

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.900
UniProt: B5Z7P5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFIYDTKSKQ KVPFEPLVEK KANIYVCGPT VYDDAHLGHA RSAIAFDLLR RTLELSGYEV MLVRNFTDID DKIINKAFKE NKSIQELSSV YIESYTRDLN ALNVKKPSLE PKASEYLDAM VRMIETLLEK NIAYRVSNGD IYLDTTKDKD YGSLSVHNSS MEFSRIGLVQ EKRLEQDFVL WKSYKGDNDV GFDSPLGKGR PGWHIECSSM VFETLALANA PYQIDIHAGG ADLLFPHHEN EACQTRCAFG VELAKYWMHN GFVNINNEKM SKSLGNSFFI KDALKNYDGE ILRNYLLGVH YRSVLNFNEE DLLVSKKRLD KIYRLKQRVS GTLGGINPNF KKEILECMQD DLNVSKALSV LESMLSSTNE KLDQNPKNKA LKGEILANLK FIEELLGIGF KDPSAYFQLG VSESEKQEIE NKIEERKRAK EQKDFLKADS IREELLNHKI ALMDTPQGTI WEKLF
NT Sequence
atgttcattt atgataccaa atcaaaacaa aaagtccctt ttgagccttt agttgaaaaa aaggcgaata tttatgtgtg cgggcctacg gtgtatgatg acgctcattt agggcatgcc aggagcgcga ttgcttttga tttgttaagg cgcacgcttg agttgagcgg ctatgaagtg atgctagtaa ggaatttcac ggacattgac gataaaatca tcaacaaagc cttcaaagaa aacaaaagca ttcaagaatt aagcagcgtt tatattgaat cttacacgag ggatttgaac gctttgaacg tgaaaaaacc cagcctagag cctaaagcga gcgagtattt agacgctatg gtgcgcatga ttgaaacgct tttagaaaaa aatatcgctt atcgggtctc taatggggat atttatttag acacgaccaa ggataaagat tacggctctt tgagcgtgca taatagcagt atggaattta gccgtattgg tttggtgcaa gaaaaacggc ttgagcagga ttttgtgcta tggaaaagct ataaggggga taatgatgtg ggctttgata gccctttagg caaagggcgc cctggctggc atatagaatg ctctagcatg gtttttgaaa ctttagcgct cgctaacgcc ccttatcaga ttgacatcca tgctggcgga gcggatttgt tattccccca ccatgaaaat gaagcgtgcc aaacccgttg cgcctttggc gtggagcttg ccaaatactg gatgcataac ggctttgtga atattaataa tgaaaaaatg tctaaaagtt tagggaatag ctttttcatt aaagatgctc tcaaaaacta tgatggtgaa attttgcgca attacttgct aggggtgcat tatcgctctg ttttgaattt caatgaagaa gacttgttag tgagtaaaaa acgcttggat aaaatctatc gtttgaaaca gcgcgtttca gggactttgg gaggaataaa tccaaatttt aaaaaagaaa ttttagagtg catgcaagat gatttaaacg tttctaaagc gttgagcgtt ttagaaagca tgctttcttc tacgaatgaa aaactggatc aaaaccccaa aaacaaggct ttgaagggcg aaattttagc gaatttgaaa ttcatagaag aactgcttgg tattgggttt aaagacccta gcgcgtattt ccagttaggc gtgagcgaga gcgaaaaaca agaaattgaa aacaagatag aagaaagaaa acgcgccaaa gaacaaaaag attttttaaa agccgatagc atcagagaag agcttttaaa ccacaaaatc gctttgatgg acaccccaca aggcacgatt tgggagaagc ttttttaa
Details for HepyC.00133.a.B1.GE40848
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMF IYDTKSKQKV PFEPLVEKKA NIYVCGPTVY DDAHLGHARS AIAFDLLRRT LELSGYEVML VRNFTDIDDK IINKAFKENK SIQELSSVYI ESYTRDLNAL NVKKPSLEPK ASEYLDAMVR MIETLLEKNI AYRVSNGDIY LDTTKDKDYG SLSVHNSSME FSRIGLVQEK RLEQDFVLWK SYKGDNDVGF DSPLGKGRPG WHIECSSMVF ETLALANAPY QIDIHAGGAD LLFPHHENEA CQTRCAFGVE LAKYWMHNGF VNINNEKMSK SLGNSFFIKD ALKNYDGEIL RNYLLGVHYR SVLNFNEEDL LVSKKRLDKI YRLKQRVSGT LGGINPNFKK EILECMQDDL NVSKALSVLE SMLSSTNEKL DQNPKNKALK GEILANLKFI EELLGIGFKD PSAYFQLGVS ESEKQEIENK IEERKRAKEQ KDFLKADSIR EELLNHKIAL MDTPQ
Validated NT Sequence
ccttgtgggg tgtccatcaa agcgattttg tggtttaaaa gctcttctct gatgctatcg gcttttaaaa aatctttttg ttctttggcg cgttttcttt cttctatctt gttttcaatt tcttgttttt cgctctcgct cacgcctaac tggaaatacg cgctagggtc tttaaaccca ataccaagca gttcttctat gaatttcaaa ttcgctaaaa tttcgccctt caaagccttg tttttggggt tttgatccag tttttcattc gtagaagaaa gcatgctttc taaaacgctc aacgctttag aaacgtttaa atcatcttgc atgcactcta aaatttcttt tttaaaattt ggatttattc ctcccaaagt ccctgaaacg cgctgtttca aacgatagat tttatccaag cgttttttac tcactaacaa gtcttcttca ttgaaattca aaacagagcg ataatgcacc cctagcaagt aattgcgcaa aatttcacca tcatagtttt tgagagcatc tttaatgaaa aagctattcc ctaaactttt agacattttt tcattattaa tattcacaaa gccgttatgc atccagtatt tggcaagctc cacgccaaag gcgcaacggg tttggcacgc ttcattttca tggtggggga ataacaaatc cgctccgcca gcatggatgt caatctgata aggggcgtta gcgagcgcta aagtttcaaa aaccatgcta gagcattcta tatgccagcc agggcgccct ttgcctaaag ggctatcaaa gcccacatca ttatccccct tatagctttt ccatagcaca aaatcctgct caagccgttt ttcttgcacc aaaccaatac ggctaaattc catactgcta ttatgcacgc tcaaagagcc gtaatcttta tccttggtcg tgtctaaata aatatcccca ttagagaccc gataagcgat atttttttct aaaagcgttt caatcatgcg caccatagcg tctaaatact cgctcgcttt aggctctagg ctgggttttt tcacgttcaa agcgttcaaa tccctcgtgt aagattcaat ataaacgctg cttaattctt gaatgctttt gttttctttg aaggctttgt tgatgatttt atcgtcaatg tccgtgaaat tccttactag catcacttca tagccgctca actcaagcgt gcgccttaac aaatcaaaag caatcgcgct cctggcatgc cctaaatgag cgtcatcata caccgtaggc ccgcacacat aaatattcgc ctttttttca actaaaggct caaaagggac tttttgtttt gatttggtat cataaatgaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMF IYDTKSKQKV PFEPLVEKKA NIYVCGPTVY DDAHLGHARS AIAFDLLRRT LELSGYEVML VRNFTDIDDK IINKAFKENK SIQELSSVYI ESYTRDLNAL NVKKPSLEPK ASEYLDAMVR MIETLLEKNI AYRVSNGDIY LDTTKDKDYG SLSVHNSSME FSRIGLVQEK RLEQDFVLWK SYKGDNDVGF DSPLGKGRPG WHIECSSMVF ETLALANAPY QIDIHAGGAD LLFPHHENEA CQTRCAFGVE LAKYWMHNGF VNINNEKMSK SLGNSFFIKD ALKNYDGEIL RNYLLGVHYR SVLNFNEEDL LVSKKRLDKI YRLKQRVSGT LGGINPNFKK EILECMQDDL NVSKALSVLE SMLSSTNEKL DQNPKNKALK GEILANLKFI EELLGIGFKD PSAYFQLGVS ESEKQEIENK IEERKRAKEQ KDFLKADSIR EELLNHKIAL MDTPQGTIWE KLF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttca tttatgatac caaatcaaaa caaaaagtcc cttttgagcc tttagttgaa aaaaaggcga atatttatgt gtgcgggcct acggtgtatg atgacgctca tttagggcat gccaggagcg cgattgcttt tgatttgtta aggcgcacgc ttgagttgag cggctatgaa gtgatgctag taaggaattt cacggacatt gacgataaaa tcatcaacaa agccttcaaa gaaaacaaaa gcattcaaga attaagcagc gtttatattg aatcttacac gagggatttg aacgctttga acgtgaaaaa acccagccta gagcctaaag cgagcgagta tttagacgct atggtgcgca tgattgaaac gcttttagaa aaaaatatcg cttatcgggt ctctaatggg gatatttatt tagacacgac caaggataaa gattacggct ctttgagcgt gcataatagc agtatggaat ttagccgtat tggtttggtg caagaaaaac ggcttgagca ggattttgtg ctatggaaaa gctataaggg ggataatgat gtgggctttg atagcccttt aggcaaaggg cgccctggct ggcatataga atgctctagc atggtttttg aaactttagc gctcgctaac gccccttatc agattgacat ccatgctggc ggagcggatt tgttattccc ccaccatgaa aatgaagcgt gccaaacccg ttgcgccttt ggcgtggagc ttgccaaata ctggatgcat aacggctttg tgaatattaa taatgaaaaa atgtctaaaa gtttagggaa tagctttttc attaaagatg ctctcaaaaa ctatgatggt gaaattttgc gcaattactt gctaggggtg cattatcgct ctgttttgaa tttcaatgaa gaagacttgt tagtgagtaa aaaacgcttg gataaaatct atcgtttgaa acagcgcgtt tcagggactt tgggaggaat aaatccaaat tttaaaaaag aaattttaga gtgcatgcaa gatgatttaa acgtttctaa agcgttgagc gttttagaaa gcatgctttc ttctacgaat gaaaaactgg atcaaaaccc caaaaacaag gctttgaagg gcgaaatttt agcgaatttg aaattcatag aagaactgct tggtattggg tttaaagacc ctagcgcgta tttccagtta ggcgtgagcg agagcgaaaa acaagaaatt gaaaacaaga tagaagaaag aaaacgcgcc aaagaacaaa aagatttttt aaaagccgat agcatcagag aagagctttt aaaccacaaa atcgctttga tggacacccc acaaggcacg atttgggaga agcttttttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for HepyC.00133.a.B1.PS38169
PURIFICATION DATe: 1/11/2017
CONCENTRATION: 24.08mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF IYDTKSKQKV PFEPLVEKKA NIYVCGPTVY DDAHLGHARS AIAFDLLRRT LELSGYEVML VRNFTDIDDK IINKAFKENK SIQELSSVYI ESYTRDLNAL NVKKPSLEPK ASEYLDAMVR MIETLLEKNI AYRVSNGDIY LDTTKDKDYG SLSVHNSSME FSRIGLVQEK RLEQDFVLWK SYKGDNDVGF DSPLGKGRPG WHIECSSMVF ETLALANAPY QIDIHAGGAD LLFPHHENEA CQTRCAFGVE LAKYWMHNGF VNINNEKMSK SLGNSFFIKD ALKNYDGEIL RNYLLGVHYR SVLNFNEEDL LVSKKRLDKI YRLKQRVSGT LGGINPNFKK EILECMQDDL NVSKALSVLE SMLSSTNEKL DQNPKNKALK GEILANLKFI EELLGIGFKD PSAYFQLGVS ESEKQEIENK IEERKRAKEQ KDFLKADSIR EELLNHKIAL MDTPQ
Validated NT Sequence
ccttgtgggg tgtccatcaa agcgattttg tggtttaaaa gctcttctct gatgctatcg gcttttaaaa aatctttttg ttctttggcg cgttttcttt cttctatctt gttttcaatt tcttgttttt cgctctcgct cacgcctaac tggaaatacg cgctagggtc tttaaaccca ataccaagca gttcttctat gaatttcaaa ttcgctaaaa tttcgccctt caaagccttg tttttggggt tttgatccag tttttcattc gtagaagaaa gcatgctttc taaaacgctc aacgctttag aaacgtttaa atcatcttgc atgcactcta aaatttcttt tttaaaattt ggatttattc ctcccaaagt ccctgaaacg cgctgtttca aacgatagat tttatccaag cgttttttac tcactaacaa gtcttcttca ttgaaattca aaacagagcg ataatgcacc cctagcaagt aattgcgcaa aatttcacca tcatagtttt tgagagcatc tttaatgaaa aagctattcc ctaaactttt agacattttt tcattattaa tattcacaaa gccgttatgc atccagtatt tggcaagctc cacgccaaag gcgcaacggg tttggcacgc ttcattttca tggtggggga ataacaaatc cgctccgcca gcatggatgt caatctgata aggggcgtta gcgagcgcta aagtttcaaa aaccatgcta gagcattcta tatgccagcc agggcgccct ttgcctaaag ggctatcaaa gcccacatca ttatccccct tatagctttt ccatagcaca aaatcctgct caagccgttt ttcttgcacc aaaccaatac ggctaaattc catactgcta ttatgcacgc tcaaagagcc gtaatcttta tccttggtcg tgtctaaata aatatcccca ttagagaccc gataagcgat atttttttct aaaagcgttt caatcatgcg caccatagcg tctaaatact cgctcgcttt aggctctagg ctgggttttt tcacgttcaa agcgttcaaa tccctcgtgt aagattcaat ataaacgctg cttaattctt gaatgctttt gttttctttg aaggctttgt tgatgatttt atcgtcaatg tccgtgaaat tccttactag catcacttca tagccgctca actcaagcgt gcgccttaac aaatcaaaag caatcgcgct cctggcatgc cctaaatgag cgtcatcata caccgtaggc ccgcacacat aaatattcgc ctttttttca actaaaggct caaaagggac tttttgtttt gatttggtat cataaatgaa catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMF IYDTKSKQKV PFEPLVEKKA NIYVCGPTVY DDAHLGHARS AIAFDLLRRT LELSGYEVML VRNFTDIDDK IINKAFKENK SIQELSSVYI ESYTRDLNAL NVKKPSLEPK ASEYLDAMVR MIETLLEKNI AYRVSNGDIY LDTTKDKDYG SLSVHNSSME FSRIGLVQEK RLEQDFVLWK SYKGDNDVGF DSPLGKGRPG WHIECSSMVF ETLALANAPY QIDIHAGGAD LLFPHHENEA CQTRCAFGVE LAKYWMHNGF VNINNEKMSK SLGNSFFIKD ALKNYDGEIL RNYLLGVHYR SVLNFNEEDL LVSKKRLDKI YRLKQRVSGT LGGINPNFKK EILECMQDDL NVSKALSVLE SMLSSTNEKL DQNPKNKALK GEILANLKFI EELLGIGFKD PSAYFQLGVS ESEKQEIENK IEERKRAKEQ KDFLKADSIR EELLNHKIAL MDTPQGTIWE KLF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCA TTTATGATAC CAAATCAAAA CAAAAAGTCC CTTTTGAGCC TTTAGTTGAA AAAAAGGCGA ATATTTATGT GTGCGGGCCT ACGGTGTATG ATGACGCTCA TTTAGGGCAT GCCAGGAGCG CGATTGCTTT TGATTTGTTA AGGCGCACGC TTGAGTTGAG CGGCTATGAA GTGATGCTAG TAAGGAATTT CACGGACATT GACGATAAAA TCATCAACAA AGCCTTCAAA GAAAACAAAA GCATTCAAGA ATTAAGCAGC GTTTATATTG AATCTTACAC GAGGGATTTG AACGCTTTGA ACGTGAAAAA ACCCAGCCTA GAGCCTAAAG CGAGCGAGTA TTTAGACGCT ATGGTGCGCA TGATTGAAAC GCTTTTAGAA AAAAATATCG CTTATCGGGT CTCTAATGGG GATATTTATT TAGACACGAC CAAGGATAAA GATTACGGCT CTTTGAGCGT GCATAATAGC AGTATGGAAT TTAGCCGTAT TGGTTTGGTG CAAGAAAAAC GGCTTGAGCA GGATTTTGTG CTATGGAAAA GCTATAAGGG GGATAATGAT GTGGGCTTTG ATAGCCCTTT AGGCAAAGGG CGCCCTGGCT GGCATATAGA ATGCTCTAGC ATGGTTTTTG AAACTTTAGC GCTCGCTAAC GCCCCTTATC AGATTGACAT CCATGCTGGC GGAGCGGATT TGTTATTCCC CCACCATGAA AATGAAGCGT GCCAAACCCG TTGCGCCTTT GGCGTGGAGC TTGCCAAATA CTGGATGCAT AACGGCTTTG TGAATATTAA TAATGAAAAA ATGTCTAAAA GTTTAGGGAA TAGCTTTTTC ATTAAAGATG CTCTCAAAAA CTATGATGGT GAAATTTTGC GCAATTACTT GCTAGGGGTG CATTATCGCT CTGTTTTGAA TTTCAATGAA GAAGACTTGT TAGTGAGTAA AAAACGCTTG GATAAAATCT ATCGTTTGAA ACAGCGCGTT TCAGGGACTT TGGGAGGAAT AAATCCAAAT TTTAAAAAAG AAATTTTAGA GTGCATGCAA GATGATTTAA ACGTTTCTAA AGCGTTGAGC GTTTTAGAAA GCATGCTTTC TTCTACGAAT GAAAAACTGG ATCAAAACCC CAAAAACAAG GCTTTGAAGG GCGAAATTTT AGCGAATTTG AAATTCATAG AAGAACTGCT TGGTATTGGG TTTAAAGACC CTAGCGCGTA TTTCCAGTTA GGCGTGAGCG AGAGCGAAAA ACAAGAAATT GAAAACAAGA TAGAAGAAAG AAAACGCGCC AAAGAACAAA AAGATTTTTT AAAAGCCGAT AGCATCAGAG AAGAGCTTTT AAACCACAAA ATCGCTTTGA TGGACACCCC ACAAGGCACG ATTTGGGAGA AGCTTTTTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT