HepyC.00042.a

Elongation factor Tu (EF-Tu)

CENTER ID: HepyC.00042.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00042.a.B1.GE40841 full length 1 399
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00042.a.B1.PS38164 full length 1 399

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|563041.6.peg.1252
UniProt: B5Z8K3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKEKFNRTK PHVNIGTIGH VDHGKTTLSA AISAVLSLKG LAEMKDYDNI DNAPEEKERG ITIATSHIEY ETENRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI LLSRQVGVPH IVVFLNKQDM VDDQELLELV EMEVRELLSA YEFPGDDTPI IAGSALRALE EAKAGNVGEW GEKVLKLMAE VDAYIPTPER DTEKTFLMPV EDVFSIAGRG TVVTGRIERG VVKVGDEVEI VGIRATQKTT VTGVEMFRKE LEKGEAGDNV GVLLRGTKKE EVERGMVLCK PGSITPHKKF EGEIYVLSKE EGGRHTPFFT NYRPQFYVRT TDVTGSITLP EGVEMVMPGD NVKITVELIS PVALELGTKF AIREGGRTVG AGVVSNIIE
NT Sequence
atggcaaaag aaaagtttaa tagaactaag ccgcatgtta atattggaac cattgggcat gtagaccatg gtaaaacgac tttgagtgca gcgatttcag cggtgctttc tttgaaaggt cttgcagaaa tgaaagacta tgataatatt gataacgccc ctgaagaaaa agaaagaggg atcactatcg ctacttctca cattgaatat gagactgaaa acagacacta tgcgcatgtg gattgcccag gacacgctga ctatgtaaaa aacatgatca ccggtgcggc gcaaatggac ggagcgattt tggttgtttc tgcagctgat ggccctatgc ctcaaaccag ggagcatatc ttattgtctc gtcaagtagg cgtgcctcac atcgttgttt tcttaaacaa acaagacatg gtagatgacc aagaattgtt agagcttgta gaaatggaag tgcgcgaatt gttgagtgcg tatgaattcc ctggtgatga cactcctatc atagcgggtt cagctttaag agctttagaa gaagcaaagg ctggcaatgt gggtgaatgg ggtgaaaaag tgcttaaact tatggctgaa gtggatgcct atatccctac tccagaaaga gacactgaaa aaactttctt gatgccagtt gaagatgtgt tctctattgc gggtagaggg actgtggtta caggtaggat tgaaagaggt gtggtgaaag taggcgatga agtggaaatc gttggtatca gagctacaca aaaaacgact gtaaccggtg tggaaatgtt tagaaaagag ttggaaaaag gtgaggccgg cgataatgtg ggcgtgcttt tgagaggaac taaaaaagaa gaagtagagc gcggtatggt tctatgcaaa ccaggttcta tcactccgca caagaaattt gagggagaaa tttatgtcct ttctaaagaa gaaggcggga gacacactcc attcttcacc aactaccgcc cacagttcta tgtgcgcacg actgatgtga ctggatctat cactcttcct gaaggcgtag aaatggttat gcctggcgat aatgtgaaaa tcactgtaga gttgattagc cctgttgcgt tagagttggg aactaaattt gcgattcgtg aaggcggtag gaccgttggt gctggtgttg tgagcaatat tattgaataa
Details for HepyC.00042.a.B1.GE40841
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA KEKFNRTKPH VNIGTIGHVD HGKTTLSAAI SAVLSLKGLA EMKDYDNIDN APEEKERGIT IATSHIEYET ENRHYAHVDC PGHADYVKNM ITGAAQMDGA ILVVSAADGP MPQTREHILL SRQVGVPHIV VFLNKQDMVD DQELLELVEM EVRELLSAYE FPGDDTPIIA GSALRALEEA KAGNVGEWGE KVLKLMAEVD AYIPTPERDT EKTFLMPVED VFSIAGRGTV VTGRIERGVV KVGDEVEIVG IRATQKTTVT GVEMFRKELE KGEAGDNVGV LLRGTKKEEV ERGMVLCKPG SITPHKKFEG EIYVLSKEEG GRHTPFFTNY RPQFYVRTTD VTGSITLPEG VEMVMPGDNV KITVELISPV ALELGTKFAI REGGRTVGA
Validated NT Sequence
ccagcaccaa cggtcctacc gccttcacga atcgcaaatt tagttcccaa ctctaacgca acagggctaa tcaactctac agtgattttc acattatcgc caggcataac catttctacg ccttcaggaa gagtgataga tccagtcaca tcagtcgtgc gcacatagaa ctgtgggcgg tagttggtga agaatggagt gtgtctcccg ccttcttctt tagaaaggac ataaatttct ccctcaaatt tcttgtgcgg agtgatagaa cctggtttgc atagaaccat accgcgctct acttcttctt ttttagttcc tctcaaaagc acgcccacat tatcgccggc ctcacctttt tccaactctt ttctaaacat ttccacaccg gttacagtcg ttttttgtgt agctctgata ccaacgattt ccacttcatc gcctactttc accacacctc tttcaatcct acctgtaacc acagtccctc tacccgcaat agagaacaca tcttcaactg gcatcaagaa agttttttca gtgtctcttt ctggagtagg gatataggca tccacttcag ccataagttt aagcactttt tcaccccatt cacccacatt gccagccttt gcttcttcta aagctcttaa agctgaaccc gctatgatag gagtgtcatc accagggaat tcatacgcac tcaacaattc gcgcacttcc atttctacaa gctctaacaa ttcttggtca tctaccatgt cttgtttgtt taagaaaaca acgatgtgag gcacgcctac ttgacgagac aataagatat gctccctggt ttgaggcata gggccatcag ctgcagaaac aaccaaaatc gctccgtcca tttgcgccgc accggtgatc atgtttttta catagtcagc gtgtcctggg caatccacat gcgcatagtg tctgttttca gtctcatatt caatgtgaga agtagcgata gtgatccctc tttctttttc ttcaggggcg ttatcaatat tatcatagtc tttcatttct gcaagacctt tcaaagaaag caccgctgaa atcgctgcac tcaaagtcgt tttaccatgg tctacatgcc caatggttcc aatattaaca tgcggcttag ttctattaaa cttttctttt gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMA KEKFNRTKPH VNIGTIGHVD HGKTTLSAAI SAVLSLKGLA EMKDYDNIDN APEEKERGIT IATSHIEYET ENRHYAHVDC PGHADYVKNM ITGAAQMDGA ILVVSAADGP MPQTREHILL SRQVGVPHIV VFLNKQDMVD DQELLELVEM EVRELLSAYE FPGDDTPIIA GSALRALEEA KAGNVGEWGE KVLKLMAEVD AYIPTPERDT EKTFLMPVED VFSIAGRGTV VTGRIERGVV KVGDEVEIVG IRATQKTTVT GVEMFRKELE KGEAGDNVGV LLRGTKKEEV ERGMVLCKPG SITPHKKFEG EIYVLSKEEG GRHTPFFTNY RPQFYVRTTD VTGSITLPEG VEMVMPGDNV KITVELISPV ALELGTKFAI REGGRTVGAG VVSNIIE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa aagaaaagtt taatagaact aagccgcatg ttaatattgg aaccattggg catgtagacc atggtaaaac gactttgagt gcagcgattt cagcggtgct ttctttgaaa ggtcttgcag aaatgaaaga ctatgataat attgataacg cccctgaaga aaaagaaaga gggatcacta tcgctacttc tcacattgaa tatgagactg aaaacagaca ctatgcgcat gtggattgcc caggacacgc tgactatgta aaaaacatga tcaccggtgc ggcgcaaatg gacggagcga ttttggttgt ttctgcagct gatggcccta tgcctcaaac cagggagcat atcttattgt ctcgtcaagt aggcgtgcct cacatcgttg ttttcttaaa caaacaagac atggtagatg accaagaatt gttagagctt gtagaaatgg aagtgcgcga attgttgagt gcgtatgaat tccctggtga tgacactcct atcatagcgg gttcagcttt aagagcttta gaagaagcaa aggctggcaa tgtgggtgaa tggggtgaaa aagtgcttaa acttatggct gaagtggatg cctatatccc tactccagaa agagacactg aaaaaacttt cttgatgcca gttgaagatg tgttctctat tgcgggtaga gggactgtgg ttacaggtag gattgaaaga ggtgtggtga aagtaggcga tgaagtggaa atcgttggta tcagagctac acaaaaaacg actgtaaccg gtgtggaaat gtttagaaaa gagttggaaa aaggtgaggc cggcgataat gtgggcgtgc ttttgagagg aactaaaaaa gaagaagtag agcgcggtat ggttctatgc aaaccaggtt ctatcactcc gcacaagaaa tttgagggag aaatttatgt cctttctaaa gaagaaggcg ggagacacac tccattcttc accaactacc gcccacagtt ctatgtgcgc acgactgatg tgactggatc tatcactctt cctgaaggcg tagaaatggt tatgcctggc gataatgtga aaatcactgt agagttgatt agccctgttg cgttagagtt gggaactaaa tttgcgattc gtgaaggcgg taggaccgtt ggtgctggtg ttgtgagcaa tattattgaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for HepyC.00042.a.B1.PS38164
PURIFICATION DATe: 1/12/2017
CONCENTRATION: 4.6mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA KEKFNRTKPH VNIGTIGHVD HGKTTLSAAI SAVLSLKGLA EMKDYDNIDN APEEKERGIT IATSHIEYET ENRHYAHVDC PGHADYVKNM ITGAAQMDGA ILVVSAADGP MPQTREHILL SRQVGVPHIV VFLNKQDMVD DQELLELVEM EVRELLSAYE FPGDDTPIIA GSALRALEEA KAGNVGEWGE KVLKLMAEVD AYIPTPERDT EKTFLMPVED VFSIAGRGTV VTGRIERGVV KVGDEVEIVG IRATQKTTVT GVEMFRKELE KGEAGDNVGV LLRGTKKEEV ERGMVLCKPG SITPHKKFEG EIYVLSKEEG GRHTPFFTNY RPQFYVRTTD VTGSITLPEG VEMVMPGDNV KITVELISPV ALELGTKFAI REGGRTVGA
Validated NT Sequence
ccagcaccaa cggtcctacc gccttcacga atcgcaaatt tagttcccaa ctctaacgca acagggctaa tcaactctac agtgattttc acattatcgc caggcataac catttctacg ccttcaggaa gagtgataga tccagtcaca tcagtcgtgc gcacatagaa ctgtgggcgg tagttggtga agaatggagt gtgtctcccg ccttcttctt tagaaaggac ataaatttct ccctcaaatt tcttgtgcgg agtgatagaa cctggtttgc atagaaccat accgcgctct acttcttctt ttttagttcc tctcaaaagc acgcccacat tatcgccggc ctcacctttt tccaactctt ttctaaacat ttccacaccg gttacagtcg ttttttgtgt agctctgata ccaacgattt ccacttcatc gcctactttc accacacctc tttcaatcct acctgtaacc acagtccctc tacccgcaat agagaacaca tcttcaactg gcatcaagaa agttttttca gtgtctcttt ctggagtagg gatataggca tccacttcag ccataagttt aagcactttt tcaccccatt cacccacatt gccagccttt gcttcttcta aagctcttaa agctgaaccc gctatgatag gagtgtcatc accagggaat tcatacgcac tcaacaattc gcgcacttcc atttctacaa gctctaacaa ttcttggtca tctaccatgt cttgtttgtt taagaaaaca acgatgtgag gcacgcctac ttgacgagac aataagatat gctccctggt ttgaggcata gggccatcag ctgcagaaac aaccaaaatc gctccgtcca tttgcgccgc accggtgatc atgtttttta catagtcagc gtgtcctggg caatccacat gcgcatagtg tctgttttca gtctcatatt caatgtgaga agtagcgata gtgatccctc tttctttttc ttcaggggcg ttatcaatat tatcatagtc tttcatttct gcaagacctt tcaaagaaag caccgctgaa atcgctgcac tcaaagtcgt tttaccatgg tctacatgcc caatggttcc aatattaaca tgcggcttag ttctattaaa cttttctttt gccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMA KEKFNRTKPH VNIGTIGHVD HGKTTLSAAI SAVLSLKGLA EMKDYDNIDN APEEKERGIT IATSHIEYET ENRHYAHVDC PGHADYVKNM ITGAAQMDGA ILVVSAADGP MPQTREHILL SRQVGVPHIV VFLNKQDMVD DQELLELVEM EVRELLSAYE FPGDDTPIIA GSALRALEEA KAGNVGEWGE KVLKLMAEVD AYIPTPERDT EKTFLMPVED VFSIAGRGTV VTGRIERGVV KVGDEVEIVG IRATQKTTVT GVEMFRKELE KGEAGDNVGV LLRGTKKEEV ERGMVLCKPG SITPHKKFEG EIYVLSKEEG GRHTPFFTNY RPQFYVRTTD VTGSITLPEG VEMVMPGDNV KITVELISPV ALELGTKFAI REGGRTVGAG VVSNIIE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA AAGAAAAGTT TAATAGAACT AAGCCGCATG TTAATATTGG AACCATTGGG CATGTAGACC ATGGTAAAAC GACTTTGAGT GCAGCGATTT CAGCGGTGCT TTCTTTGAAA GGTCTTGCAG AAATGAAAGA CTATGATAAT ATTGATAACG CCCCTGAAGA AAAAGAAAGA GGGATCACTA TCGCTACTTC TCACATTGAA TATGAGACTG AAAACAGACA CTATGCGCAT GTGGATTGCC CAGGACACGC TGACTATGTA AAAAACATGA TCACCGGTGC GGCGCAAATG GACGGAGCGA TTTTGGTTGT TTCTGCAGCT GATGGCCCTA TGCCTCAAAC CAGGGAGCAT ATCTTATTGT CTCGTCAAGT AGGCGTGCCT CACATCGTTG TTTTCTTAAA CAAACAAGAC ATGGTAGATG ACCAAGAATT GTTAGAGCTT GTAGAAATGG AAGTGCGCGA ATTGTTGAGT GCGTATGAAT TCCCTGGTGA TGACACTCCT ATCATAGCGG GTTCAGCTTT AAGAGCTTTA GAAGAAGCAA AGGCTGGCAA TGTGGGTGAA TGGGGTGAAA AAGTGCTTAA ACTTATGGCT GAAGTGGATG CCTATATCCC TACTCCAGAA AGAGACACTG AAAAAACTTT CTTGATGCCA GTTGAAGATG TGTTCTCTAT TGCGGGTAGA GGGACTGTGG TTACAGGTAG GATTGAAAGA GGTGTGGTGA AAGTAGGCGA TGAAGTGGAA ATCGTTGGTA TCAGAGCTAC ACAAAAAACG ACTGTAACCG GTGTGGAAAT GTTTAGAAAA GAGTTGGAAA AAGGTGAGGC CGGCGATAAT GTGGGCGTGC TTTTGAGAGG AACTAAAAAA GAAGAAGTAG AGCGCGGTAT GGTTCTATGC AAACCAGGTT CTATCACTCC GCACAAGAAA TTTGAGGGAG AAATTTATGT CCTTTCTAAA GAAGAAGGCG GGAGACACAC TCCATTCTTC ACCAACTACC GCCCACAGTT CTATGTGCGC ACGACTGATG TGACTGGATC TATCACTCTT CCTGAAGGCG TAGAAATGGT TATGCCTGGC GATAATGTGA AAATCACTGT AGAGTTGATT AGCCCTGTTG CGTTAGAGTT GGGAACTAAA TTTGCGATTC GTGAAGGCGG TAGGACCGTT GGTGCTGGTG TTGTGAGCAA TATTATTGAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT