HepyC.00008.a

Serine hydroxymethyltransferase (SHMT) (Serine methylase) (EC 2.1.2.1)

CENTER ID: HepyC.00008.a
ORGANISM: Helicobacter pylori G27
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
HepyC.00008.a.B1.GE40838 full length 1 416
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Structures
5VC2
DEPOSITED: 3/30/2017
DETERMINATION: XRay
CLONE: HepyC.00008.a.B1.GE40838
PROTEIN: HepyC.00008.a.B1.PS38187
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|563041.6.peg.190
UniProt: B5Z9V7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAYFLEQTDS EIFELIFEEY KRQNEHLEMI ASENYTFPSV MEAMGSILTN KYAEGYPNKR YYGGCEVVDK IESLAIERAK KLFNCQFANV QAHSGSQANN AVYHALLKPY DKILGMDLSC GGHLTHGAKV SLTGKHYQSF SYGVNLDGYI DYEEALKIAQ SVKPEIIVCG FSAYPREIDF KKFREIADEV GALLLGDIAH VAGLVVTNEH AHPFPHCHVV SSTTHKTLRG PRGGIILTND EEIAAKIDKA IFPGTQGGPL MHVIAAKAVG FKENLKPEFK AYAKLVKSNM QVLAKALKEK NHKLVSGGTS NHLLLMDFLD KPYSGKDADI ALGNAGITVN KNTIPGETRS PFVTSGIRIG SAALSARGMG AKEFEIIGNK ISDILNDINN VSLQLHVKEE LKAMANQFPV YQQPIF
NT Sequence
atggcgtatt ttttagaaca aacggatagt gaaatttttg aattaatctt tgaagaatat aagcggcaaa atgagcattt agaaatgata gcgagcgaga attacacttt tcctagtgtt atggaagcta tgggaagcat tttaacgaat aaatacgctg agggctatcc taacaagcgc tattatggag gctgtgaagt ggtggataaa atagaaagcc tggctataga aagggctaaa aagcttttta attgccagtt cgctaacgtg caagcgcatt caggctcaca agccaataac gctgtctatc acgctctttt aaagccttat gacaagattt taggcatgga tttaagctgt ggagggcatt taacgcatgg cgctaaagtg agtttaaccg gcaagcatta tcagagcttt tcttatggcg tgaatttgga tggctatatt gattatgaag aagcgctaaa gatcgctcaa agcgttaagc cagaaatcat tgtgtgcggg ttttcagcct atccaaggga gattgatttt aagaaattta gagaaatcgc tgatgaagtg ggggcgttac tattaggcga tatagcccat gtggcggggc ttgtggtaac taatgagcat gcccatcctt tcccgcattg ccatgtggtt tcaagcacca ctcataagac cttaagaggg cctagagggg ggattatttt aactaatgat gaagagatag cggctaagat tgataaagcg atttttccag ggactcaagg cgggcctttg atgcatgtga ttgctgctaa agcggtgggg tttaaagaga atctaaaacc agaatttaaa gcttacgcaa aattagtgaa atctaacatg caagttttgg ctaaagcgtt aaaagaaaaa aaccataaat tagtgagcgg tggcacttct aaccatttgc ttttaatgga tttcttagat aagccttata gcgggaaaga cgctgatatt gcattaggga atgccgggat caccgtgaat aaaaacacca ttcctggcga aacgcgcagc ccttttgtaa cgagcgggat aaggattggc tcagcggcat tgagcgcaag gggcatggga gctaaagaat ttgaaatcat agggaataaa atatcagata ttttgaatga tattaataat gttagtttgc aattgcatgt gaaagaagaa ttgaaagcca tggccaatca attccctgtg taccaacaac ctatttttta a
Details for HepyC.00008.a.B1.GE40838
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA YFLEQTDSEI FELIFEEYKR QNEHLEMIAS ENYTFPSVME AMGSILTNKY AEGYPNKRYY GGCEVVDKIE SLAIERAKKL FNCQFANVQA HSGSQANNAV YHALLKPYDK ILGMDLSCGG HLTHGAKVSL TGKHYQSFSY GVNLDGYIDY EEALKIAQSV KPEIIVCGFS AYPREIDFKK FREIADEVGA LLLGDIAHVA GLVVTNEHAH PFPHCHVVSS TTHKTLRGPR GGIILTNDEE IAAKIDKAIF PGTQGGPLMH VIAAKAVGFK ENLKPEFKAY AKLVKSNMQV LAKALKEKNH KLVSGGTSNH LLLMDFLDKP YSGKDADIAL GNAGITVNKN TIPGETRSPF VTSGIRIGSA ALSARGMGAK EFEIIGNKIS DILNDINNVS LQLHVKEELK AMANQ
Validated NT Sequence
aattgattgg ccatggcttt caattcttct ttcacatgca attgcaaact aacattatta atatcattca aaatatctga tattttattc cctatgattt caaattcttt agctcccatg ccccttgcgc tcaatgccgc tgagccaatc cttatcccgc tcgttacaaa agggctgcgc gtttcgccag gaatggtgtt tttattcacg gtgatcccgg cattccctaa tgcaatatca gcgtctttcc cgctataagg cttatctaag aaatccatta aaagcaaatg gttagaagtg ccaccgctca ctaatttatg gtttttttct tttaacgctt tagccaaaac ttgcatgtta gatttcacta attttgcgta agctttaaat tctggtttta gattctcttt aaaccccacc gctttagcag caatcacatg catcaaaggc ccgccttgag tccctggaaa aatcgcttta tcaatcttag ccgctatctc ttcatcatta gttaaaataa tcccccctct aggccctctt aaggtcttat gagtggtgct tgaaaccaca tggcaatgcg ggaaaggatg ggcatgctca ttagttacca caagccccgc cacatgggct atatcgccta atagtaacgc ccccacttca tcagcgattt ctctaaattt cttaaaatca atctcccttg gataggctga aaacccgcac acaatgattt ctggcttaac gctttgagcg atctttagcg cttcttcata atcaatatag ccatccaaat tcacgccata agaaaagctc tgataatgct tgccggttaa actcacttta gcgccatgcg ttaaatgccc tccacagctt aaatccatgc ctaaaatctt gtcataaggc tttaaaagag cgtgatagac agcgttattg gcttgtgagc ctgaatgcgc ttgcacgtta gcgaactggc aattaaaaag ctttttagcc ctttctatag ccaggctttc tattttatcc accacttcac agcctccata atagcgcttg ttaggatagc cctcagcgta tttattcgtt aaaatgcttc ccatagcttc cataacacta ggaaaagtgt aattctcgct cgctatcatt tctaaatgct cattttgccg cttatattct tcaaagatta attcaaaaat ttcactatcc gtttgttcta aaaaatacgc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMA YFLEQTDSEI FELIFEEYKR QNEHLEMIAS ENYTFPSVME AMGSILTNKY AEGYPNKRYY GGCEVVDKIE SLAIERAKKL FNCQFANVQA HSGSQANNAV YHALLKPYDK ILGMDLSCGG HLTHGAKVSL TGKHYQSFSY GVNLDGYIDY EEALKIAQSV KPEIIVCGFS AYPREIDFKK FREIADEVGA LLLGDIAHVA GLVVTNEHAH PFPHCHVVSS TTHKTLRGPR GGIILTNDEE IAAKIDKAIF PGTQGGPLMH VIAAKAVGFK ENLKPEFKAY AKLVKSNMQV LAKALKEKNH KLVSGGTSNH LLLMDFLDKP YSGKDADIAL GNAGITVNKN TIPGETRSPF VTSGIRIGSA ALSARGMGAK EFEIIGNKIS DILNDINNVS LQLHVKEELK AMANQFPVYQ QPIF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgt attttttaga acaaacggat agtgaaattt ttgaattaat ctttgaagaa tataagcggc aaaatgagca tttagaaatg atagcgagcg agaattacac ttttcctagt gttatggaag ctatgggaag cattttaacg aataaatacg ctgagggcta tcctaacaag cgctattatg gaggctgtga agtggtggat aaaatagaaa gcctggctat agaaagggct aaaaagcttt ttaattgcca gttcgctaac gtgcaagcgc attcaggctc acaagccaat aacgctgtct atcacgctct tttaaagcct tatgacaaga ttttaggcat ggatttaagc tgtggagggc atttaacgca tggcgctaaa gtgagtttaa ccggcaagca ttatcagagc ttttcttatg gcgtgaattt ggatggctat attgattatg aagaagcgct aaagatcgct caaagcgtta agccagaaat cattgtgtgc gggttttcag cctatccaag ggagattgat tttaagaaat ttagagaaat cgctgatgaa gtgggggcgt tactattagg cgatatagcc catgtggcgg ggcttgtggt aactaatgag catgcccatc ctttcccgca ttgccatgtg gtttcaagca ccactcataa gaccttaaga gggcctagag gggggattat tttaactaat gatgaagaga tagcggctaa gattgataaa gcgatttttc cagggactca aggcgggcct ttgatgcatg tgattgctgc taaagcggtg gggtttaaag agaatctaaa accagaattt aaagcttacg caaaattagt gaaatctaac atgcaagttt tggctaaagc gttaaaagaa aaaaaccata aattagtgag cggtggcact tctaaccatt tgcttttaat ggatttctta gataagcctt atagcgggaa agacgctgat attgcattag ggaatgccgg gatcaccgtg aataaaaaca ccattcctgg cgaaacgcgc agcccttttg taacgagcgg gataaggatt ggctcagcgg cattgagcgc aaggggcatg ggagctaaag aatttgaaat catagggaat aaaatatcag atattttgaa tgatattaat aatgttagtt tgcaattgca tgtgaaagaa gaattgaaag ccatggccaa tcaattccct gtgtaccaac aacctatttt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat