SSGCID: Target Page

GilaA.19931.a

Kinase, CDC7

CENTER ID:	GilaA.19931.a
ORGANISM:	Giardia lamblia ATCC 50803
ASSOCIATED DISEASE:	Giardiasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.19931.a.B2.GE42512	like segment 1 and 2 of human structure 4F99		8	420
GilaA.19931.a.B3.GE42513	like segments 1 and 2 of human structure 4F99 trimmed		14	420

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	GiardiaDB:GL50803_112076
UniProt:	A8BPA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRRHQADTG TADYTQYRQE FPEIPFDRYK ITQHVGKGAF SKVFLAYTPE FQRRAIKRIR LTAKPHQLLK EIEFLRRLEG KSHIVQIKEI RFSDATLQTT VITSYSDTDD FKSVILDFTP REIQAYMRSI LTALSHLEKL KIIHRDIKPG NFLFERKTGK GLLIDFGLAQ TLREVREFPL LRGQSKSRKG VIHINLDRLM RQSNQYLTDL TAQGEETYYT ARMSLKEGQT VPAAHVTGSN AAASTNVVRP NDDTAERLPI AGYINKDHST FRPLPPSAHS GTHGYRAPEI LVPVRNQDCK IDSWSVGVIL IQILTGKTSL FRGNPTVDIY EILAIRSIIG TDRFIQGMSR LKADFSLTAG APMPQAIALE ELAEWYNPDL LAVLPASCFD LCRKLLEFDP ESRLSATEAL SHPFLAMDEN LLPNYRFQDG AHSKSLLSFT GYANATNSQD SMNREEETLQ ALRKRGSEIA ERLGLCRVTS ETLADEYSTM SPQSMIPLPR DLRKQAMKSV QKFALDKTVQ GPSGIIVERS KTSDYSFPSN SSVQDLSSCG GVTLSRPRLA AAPGSHARHC AIDAAMRQKI AYYKEKGIRV TLDDLHREVL DQQWEIANKD KETFERAVRA TAVRRRLDPW VQPLKALDKE LRICQKDVRK AAEEYRRTVL SLGIIDLETK TADEGHRHKV SAGLDGDKDK LDAKNEQGRT QLSGATGITV TGQGAGAFTN YHSYLSALIP SPNALKTAVN IKLASDAKIQ VAKTMDVKTV ESFYYPKYYA INKIRTHRLL NFIVKSELAQ TAALDTGNSV GVAGVTTCTN HLTKTLATVI ATKAWEVVHS NSSKNMITKE KSRSVYDLTM KAAKIASVSH RLALSGPDAW EAKGESFFLS PNSRLGMIEI KADECKFRVH DFNTKILQHQ HHASQCQFII QERTGFLSKI AQLEESIRSK MNANTEQIKI LQGTLKSSAN KNDTIQEEIN YLTAQKEQQE AAISKFVAVV ADNKTLIAQH KESYANLTSE LSVMIANKNE WVKEYKQIYE RDIKDADYSF GEEHEHLLEN FSDCWSETIS VSDEGVEARP AFKPFGVYNP SELQEPYFTS MDEMKTALRQ QDAAMDRRKS RREQRRRLEC LIAKRRDPIL EQLRQMNDDI QTARAELLDS MITGEGISPA PTDKTDKTKH NSLMPKQALS VSPSMLAQAV AIKSLAAKYA TVMGFFTKHT LSGAYCVTST FEPGRTRQPA EVGLLSFRNG NITGEMHQTD GGAKRASSTG PNSRGNTTVA GMSTNASINP NANRGSAGGL SNDNKQLHAL KPYARNALAY ISQQLEVERK AHALLTLISS PYGRYLMPFH KILSDGAPFP SGARSNSFYI MCSKSDLFIP AELQEAILTL QNLSERYFRT SIGRKDLYLI AAACQTELDL RAQNATNTNN PNNANNELQH LGLSRRPPNL DYMLLLQGKL NNLIPYFEDT RSTSAISGLN KSATHRSLAD PGRTSQRYPQ TCKYIQTILE QVMRRFVAMP GVLSGLYEEC IEGISNIKGR ILEAVVIELI NILQTGSTEN VDRVRGGCSF YENNVMLLRC LRLLQTAINT LGAGTVFEIL RGLVYPVTLL RGSGFINGVE KTQKPVAESD SPDKKPRRFN YLTEEDVKSV AKMVSKPENI TGSFDASLLQ KIDDSNARRR LLLQTQYGGR HHLNAIRQRR RRAEIYF

NT Sequence

atgacccgac gccaccaggc cgacacggga accgcggact acacgcagta cagacaggaa ttcccagaga ttccattcga caggtacaag ataacgcagc acgtagggaa gggcgcgttc tcaaaagttt tcttggccta tacaccagag ttccaaaggc gtgccatcaa gcgcatcaga ttgacagcca aaccgcacca gctcctcaag gaaatagagt ttctccgtcg attggagggg aagtcgcata tcgtgcaaat caaagaaatt cgcttctctg acgccacgct gcaaacaacc gtcatcactt cctactcaga cacggatgat ttcaagtctg ttatactgga cttcacccca agagaaatcc aggcctatat gcgctccatt ttgactgccc tctctcatct agagaaattg aaaataatac atcgtgatat aaagcctggc aatttcctct ttgagcgaaa aacaggaaaa gggctgctta ttgattttgg cctcgcccaa acgctacgcg aggtccgaga gtttccgctt ctccgaggcc agtcgaagtc gcgcaagggt gtcatacaca ttaacttaga ccgtcttatg cgtcaaagca atcagtatct gaccgacctg acagcccagg gagaggagac atactacact gcgcgcatgt ctctcaaaga aggacagaca gtaccggctg cacatgtcac aggctcgaat gctgccgcat caacgaatgt tgttcgaccc aatgacgaca cggctgagcg acttcccata gcgggctaca taaacaagga tcattcaacc ttccgcccgc ttccaccatc ggcacatagt ggaactcacg ggtacagagc gcctgagatc cttgttccag ttcgaaacca ggattgtaag attgatagct ggagtgtcgg tgttattctt atacagatcc tgacggggaa aacgtcgctt ttccgtggca atccgactgt ggatatttat gagatcctgg ctattcgctc gataattgga acagacaggt tcattcaggg catgtccagg ctcaaggctg actttagtct cacagctggt gctccgatgc cgcaagctat cgccctggaa gagcttgctg aatggtataa tccggatctc ctggcagtac tgcctgcttc ctgctttgat ctgtgcagga agctgcttga gtttgatcct gaaagccgac taagtgcaac agaggctctt tcacacccct tcctcgccat ggacgaaaac ctacttccta attaccgctt ccaggatggc gcccacagca agagcctttt aagttttaca ggatatgcca atgcaacgaa cagtcaggac agcatgaacc gtgaggagga gactctgcag gctcttcgta aacgcggaag tgagatcgct gagcgactcg gtctttgccg cgtgacttcc gagaccttag ccgatgaata ttccaccatg tcgccacagt ctatgatccc tctaccccgt gacttgagaa agcaagctat gaaatcagtc caaaagtttg ccttggataa gacagtacag gggccgtctg ggattattgt agaacgctca aagacaagcg actacagctt cccgagcaat tcttcggtgc aagacctttc ctcgtgcggg ggcgtgacat tgagccgtcc gcgtcttgcc gcagcccctg gtagccatgc aagacactgt gcaattgatg cagcgatgcg gcagaagata gcatattaca aggagaaggg catcagggtt accctggatg atctacatcg agaggtgctt gaccaacaat gggagatagc aaacaaggac aaggagacct tcgaacgcgc tgtgcgtgct acagcagtgc gacgaagact ggatccgtgg gttcagcctc ttaaagcctt agataaagag ctcaggatct gccagaagga tgtgcggaag gctgctgaag aatatcgacg aaccgttctc tctcttggta ttatagatct ggaaaccaag accgccgatg aaggccaccg ccacaaagtt tctgccggac tggacggcga caaggacaag ctagatgcaa agaacgagca aggacgcact caactttctg gtgcaactgg aattactgtt actgggcagg gggcaggagc tttcacaaac tatcacagtt atttgtcggc tcttattccg tccccgaatg ctcttaagac agctgtgaat ataaagctcg catcagatgc caagattcaa gttgcgaaga ctatggacgt aaaaacagtg gagtctttct attatccgaa gtactatgcc atcaataaga ttagaacgca tcgcctgctt aattttatcg tcaagtctga gctggcgcag actgcagccc ttgacactgg gaatagtgtt ggagttgcag gcgtgaccac ctgcacaaac catctgacta agacccttgc aactgtgata gctacgaaag catgggaagt tgttcactcc aattcttcca agaatatgat cactaaggag aagtcccggt cagtatacga cttgacaatg aaggctgcca agatcgccag tgtttctcat cgccttgcac tatctggccc tgatgcatgg gaagcaaagg gagagtcctt cttcttgtca cctaattctc gattagggat gatagagatc aaagctgatg agtgcaagtt tcgtgtccac gactttaaca caaagatcct tcaacatcag catcacgctt cccagtgcca atttatcatt caggagcgca caggtttcct cagtaagata gcacagcttg aagagtccat aaggagcaaa atgaacgcaa atacagaaca gattaagatt ttgcaaggta ctctgaagtc gtctgccaat aagaacgaca caattcagga ggagataaac tatcttacgg ctcagaaaga acagcaggag gcggccatct ccaagtttgt agcggtcgtt gctgacaata aaacgctcat tgctcaacat aaggaaagct acgcaaatct tacttccgaa ctttcagtaa tgattgcaaa caaaaatgaa tgggtgaagg aatacaagca aatatatgag cgggatataa aggatgctga ctactccttt ggggaggaac atgagcacct tcttgagaac ttttcagatt gctggagcga gaccatttct gtgagtgacg agggagttga ggcacgacct gcattcaagc catttggagt gtataatcct tcggagctcc aagaaccata cttcacctcc atggacgaga tgaagacagc tctaaggcag caggatgccg caatggaccg acgaaagagc agaagagagc agcgtcgtcg gctagagtgt ctgatagcaa aaagaagaga ccctatttta gagcagcttc gtcaaatgaa cgacgatatc cagactgctc gcgcagaact tctggactca atgatcactg gagaaggaat ttctcccgcg ccaacagaca aaacggacaa gactaagcac aattctttga tgccaaagca ggcgctgtct gtgtcgccgt ccatgctcgc acaagctgtt gcaataaagt ctcttgctgc caaatacgct actgtcatgg gcttcttcac taaacacaca cttagtgggg catactgtgt tacctccact tttgagcctg ggcgtactcg acaacctgca gaggtaggat tgctaagttt ccgaaacgga aacatcactg gagagatgca ccagactgat ggaggcgcta agcgtgcatc gtctaccggt cctaattcac gcggcaatac aactgtcgcc ggaatgtcta caaatgcatc cattaatccc aatgcaaata gagggtctgc aggaggttta tccaatgata ataagcagct tcatgctctt aaaccttatg ctcggaatgc ccttgcttac atttcacaac aactggaggt tgagcgaaag gcgcatgcct tacttacgct gatatcatct ccctatggac gttatctaat gcctttccat aaaatactat cggacggggc accgttcccc agtggagctc ggtcgaactc cttttatatt atgtgttcaa agagtgatct atttattcca gcagagctcc aggaagccat cttaacgctg cagaatcttt cggaacgcta tttccgcaca tctattggtc gtaaagacct ttatctaatt gcagcagctt gtcagactga actggatctg cgagcccaaa atgcgaccaa cactaataat cccaataatg ccaataatga acttcagcac cttggattga gcaggaggcc tccaaacttg gattacatgc ttcttttgca agggaagcta aacaacttga tcccttattt tgaggacacc cgctctacgt ctgccatctc cggtcttaat aaaagcgcca cgcaccgatc tcttgctgac cccggcagga cgtcgcagcg ctacccccag acctgcaagt acatccagac catcctggag caggtgatgc gccgctttgt ggcgatgccc ggggtccttt cgggccttta tgaggagtgc attgagggca tcagcaacat caagggccgc atcctggagg ccgtcgtcat agagctgatc aatatcctgc agaccgggtc cactgaaaat gtcgataggg ttcgcggcgg ctgctccttc tacgaaaata acgtgatgct cctgcgctgc ctccgcctgc tccagaccgc cataaacacg ctgggtgccg ggacagtctt tgagatactg cgcggccttg tctaccccgt gactcttttg cgcggcagcg gcttcatcaa tggggtggag aagactcaga agccggtggc tgagtctgac agcccagaca agaagcctcg gaggttcaac tacctgacag aagaggatgt caagagcgtc gccaagatgg tgtccaagcc agagaacatt accgggagct tcgacgcatc gctgctgcag aagatagacg atagcaatgc gcgcaggcgt ctgcttctac agacacagta cggcggccgc catcacctca atgcaataag acagcgcagg cgtcgtgccg agatatactt ctaa

Details for GilaA.19931.a.B2.GE42512

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	48kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHDT GTADYTQYRQ EFPEIPFDRY KITQHVGKGA FSKVFLAYTP EFQRRAIKRI RLTAKPHQLL KEIEFLRRLE GKSHIVQIKE IRFSDATLQT TVITSYSDTD DFKSVILDFT PREIQAYMRS ILTALSHLEK LKIIHRDIKP GNFLFERKTG KGLLIDFGLA QTLREVREFP LLRGQSKSRK GVIHINLDRL MRQSNQYLTD LTAQGEETYY TARMSLKEGQ TVPAAHVTGS NAAASTNVVR PNDDTAERLP IAGYINKDHS TFRPLPPSAH SGTHGYRAPE ILVPVRNQDC KIDSWSVGVI LIQILTGKTS LFRGNPTVDI YEILAIRSII GTDRFIQGMS RLKADFSLTA GAPMPQAIAL EELAEWYNPD LLAVLPASCF DLCRKLLEFD PESRLSATEA LSHPFLAMDE N

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacacgg gaaccgcgga ctacacgcag tacagacagg aattcccaga gattccattc gacaggtaca agataacgca gcacgtaggg aagggcgcgt tctcaaaagt tttcttggcc tatacaccag agttccaaag gcgtgccatc aagcgcatca gattgacagc caaaccgcac cagctcctca aggaaataga gtttctccgt cgattggagg ggaagtcgca tatcgtgcaa atcaaagaaa ttcgcttctc tgacgccacg ctgcaaacaa ccgtcatcac ttcctactca gacacggatg atttcaagtc tgttatactg gacttcaccc caagagaaat ccaggcctat atgcgctcca ttttgactgc cctctctcat ctagagaaat tgaaaataat acatcgtgat ataaagcctg gcaatttcct ctttgagcga aaaacaggaa aagggctgct tattgatttt ggcctcgccc aaacgctacg cgaggtccga gagtttccgc ttctccgagg ccagtcgaag tcgcgcaagg gtgtcataca cattaactta gaccgtctta tgcgtcaaag caatcagtat ctgaccgacc tgacagccca gggagaggag acatactaca ctgcgcgcat gtctctcaaa gaaggacaga cagtaccggc tgcacatgtc acaggctcga atgctgccgc atcaacgaat gttgttcgac ccaatgacga cacggctgag cgacttccca tagcgggcta cataaacaag gatcattcaa ccttccgccc gcttccacca tcggcacata gtggaactca cgggtacaga gcgcctgaga tccttgttcc agttcgaaac caggattgta agattgatag ctggagtgtc ggtgttattc ttatacagat cctgacgggg aaaacgtcgc ttttccgtgg caatccgact gtggatattt atgagatcct ggctattcgc tcgataattg gaacagacag gttcattcag ggcatgtcca ggctcaaggc tgactttagt ctcacagctg gtgctccgat gccgcaagct atcgccctgg aagagcttgc tgaatggtat aatccggatc tcctggcagt actgcctgct tcctgctttg atctgtgcag gaagctgctt gagtttgatc ctgaaagccg actaagtgca acagaggctc tttcacaccc cttcctcgcc atggacgaaa actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for GilaA.19931.a.B3.GE42513

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	47kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	68

Validated AA Sequence

MXXXXXXXXX XEKLKIIHRD IKPGNFLFER KTGKGLLIDF GLAQTLREVR EFPLLRGQSK SRKGVIHINL DRLMRQSNQY LTDLTAQGEE TYYTARMSLK EGQTVPAAHV TGSNAAASTN VVRPNDDTAE RLPIAGYINK DHSTFRPLPP SAHSGTHGYR APEILVPVRN QDCKIDSWSV GVILIQILTG KTSLFRGNPT VDIYEILAIR SIIGTDRFIQ GMSRLKADFS LTAGAPMPQA IALEELAEWY NPDLLAVLPA SCFDLCRKLL EFDPESRLSA TEALSH

Validated NT Sequence

ganntnnnnn nnnnnatgcn nnccatnnnn nctgnnnnnn nnnnnntaga gaaattgaaa ataatacatc gtgatataaa gcctggcaat ttcctctttg agcgaaaaac aggaaaaggg ctgcttattg attttggcct cgcccaaacg ctacgcgagg tccgagagtt tccgcttctc cgaggccagt cgaagtcgcg caagggtgtc atacacatta acttagaccg tcttatgcgt caaagcaatc agtatctgac cgacctgaca gcccagggag aggagacata ctacactgcg cgcatgtctc tcaaagaagg acagacagta ccggctgcac atgtcacagg ctcgaatgct gccgcatcaa cgaatgttgt tcgacccaat gacgacacgg ctgagcgact tcccatagcg ggctacataa acaaggatca ttcaaccttc cgcccgcttc caccatcggc acatagtgga actcacgggt acagagcgcc tgagatcctt gttccagttc gaaaccagga ttgtaagatt gatagctgga gtgtcggtgt tattcttata cagatcctga cggggaaaac gtcgcttttc cgtggcaatc cgactgtgga tatttatgag atcctggcta ttcgctcgat aattggaaca gacaggttca ttcagggcat gtccaggctc aaggctgact ttagtctcac agctggtgct ccgatgccgc aagctatcgc cctggaagag cttgctgaat ggtataatcc ggatctcctg gcagtactgc ctgcttcctg ctttgatctg tgcaggaagc tgcttgagtt tgatcctgaa agccgactaa gtgcaacaga ggctctttca cacc

Expected Protein Sequence

MAHHHHHHYT QYRQEFPEIP FDRYKITQHV GKGAFSKVFL AYTPEFQRRA IKRIRLTAKP HQLLKEIEFL RRLEGKSHIV QIKEIRFSDA TLQTTVITSY SDTDDFKSVI LDFTPREIQA YMRSILTALS HLEKLKIIHR DIKPGNFLFE RKTGKGLLID FGLAQTLREV REFPLLRGQS KSRKGVIHIN LDRLMRQSNQ YLTDLTAQGE ETYYTARMSL KEGQTVPAAH VTGSNAAAST NVVRPNDDTA ERLPIAGYIN KDHSTFRPLP PSAHSGTHGY RAPEILVPVR NQDCKIDSWS VGVILIQILT GKTSLFRGNP TVDIYEILAI RSIIGTDRFI QGMSRLKADF SLTAGAPMPQ AIALEELAEW YNPDLLAVLP ASCFDLCRKL LEFDPESRLS ATEALSHPFL AMDEN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattacacgc agtacagaca ggaattccca gagattccat tcgacaggta caagataacg cagcacgtag ggaagggcgc gttctcaaaa gttttcttgg cctatacacc agagttccaa aggcgtgcca tcaagcgcat cagattgaca gccaaaccgc accagctcct caaggaaata gagtttctcc gtcgattgga ggggaagtcg catatcgtgc aaatcaaaga aattcgcttc tctgacgcca cgctgcaaac aaccgtcatc acttcctact cagacacgga tgatttcaag tctgttatac tggacttcac cccaagagaa atccaggcct atatgcgctc cattttgact gccctctctc atctagagaa attgaaaata atacatcgtg atataaagcc tggcaatttc ctctttgagc gaaaaacagg aaaagggctg cttattgatt ttggcctcgc ccaaacgcta cgcgaggtcc gagagtttcc gcttctccga ggccagtcga agtcgcgcaa gggtgtcata cacattaact tagaccgtct tatgcgtcaa agcaatcagt atctgaccga cctgacagcc cagggagagg agacatacta cactgcgcgc atgtctctca aagaaggaca gacagtaccg gctgcacatg tcacaggctc gaatgctgcc gcatcaacga atgttgttcg acccaatgac gacacggctg agcgacttcc catagcgggc tacataaaca aggatcattc aaccttccgc ccgcttccac catcggcaca tagtggaact cacgggtaca gagcgcctga gatccttgtt ccagttcgaa accaggattg taagattgat agctggagtg tcggtgttat tcttatacag atcctgacgg ggaaaacgtc gcttttccgt ggcaatccga ctgtggatat ttatgagatc ctggctattc gctcgataat tggaacagac aggttcattc agggcatgtc caggctcaag gctgacttta gtctcacagc tggtgctccg atgccgcaag ctatcgccct ggaagagctt gctgaatggt ataatccgga tctcctggca gtactgcctg cttcctgctt tgatctgtgc aggaagctgc ttgagtttga tcctgaaagc cgactaagtg caacagaggc tctttcacac cccttcctcg ccatggacga aaactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat