GilaA.19525.a

3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.88)

CENTER ID: GilaA.19525.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.19525.a.B1.GE41342 full length 1 751
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_7573
UniProt: A8B8P2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDISLDYELK TGFAVETVMR TLQYPEERIN WLEEHKAINS ELGDELRAFA AVIGLDGPAQ MYGFTNDARV RSASITSPIR SAYAEMRENS GKIKEIVCEN NAKRPSSPSL VVTDPLTNAL QIALAQAKVR SDHEVSKYSR RIQALDTMSE NVIGATMLPL SVVPDVLIDG TMYTVPISTE EPSVVAALAH AAKIFRIGKG VRTLGPINPF ILGQLIFMLI DGQWKHPMNL SELESLLSRS LATHLPNSRN YGKGIVSCYL RKLNDYILLE IGLDPGEAMG ANMINGFMEA IAPDVERYLT KFGHVERLTS ILSNSGAGRE YEAIAKITVE DYAMAKNIPM KKAEDILRRI EALSQLAEMD TNRRITHNKG VLNGIIGVAL ACGQDTRAID VANCQLGHTK PLVTHRFNSK ELFLRLTARI SAPVGVIGGS SKLFWGLSIL GQAQENRISS GSLGRILAAV GLLQNFAALQ ALVTNGIQHG HMKLHNRKLV TRETPTPASA SSSISASLQA VSIEPKTTPP KVTTPFMDSQ DRDKNKLNTA LNESLKRIED IISHINYTPE EPNYEAKMML TNGESDLYRY FSPAKLSPVR NLQQPSLRMQ SPKRQSYALE KLASQTLLDR VRTRSPILSQ SISSDSPFGA ASTHQPTVLS TLAKGLGQSA STYNGGQSAD LFGSGHGKPA SSSTLTRQMS YSPNRLNSFS VVSDRTTSPV KSLEMEHWEG NSGARNELST SKRPYNIVSP SNLKHNSWRN S
NT Sequence
atggacatct cccttgatta cgaactaaaa acaggttttg cggttgaaac cgtaatgcgc actctacaat atccagaaga gcggataaat tggcttgagg agcacaaagc catcaatagt gagttgggag atgagcttcg cgcattcgca gcggttatcg gattggatgg accagcgcaa atgtacggtt ttacgaacga cgcccgcgtt cgtagcgcgt ccataacaag cccaatccgc agtgcatacg cagaaatgcg ggaaaacagt ggcaaaatta aggagatagt ttgcgagaat aatgcaaaac ggccatcaag tccaagcttg gtagtgacag acccattgac aaatgctctt cagatcgcgc ttgcacaagc aaaagtcaga agcgaccacg aagtctccaa gtactcgcgg agaatccagg ctcttgatac aatgagcgaa aacgttattg gagcgacaat gcttccattg tccgtcgttc ccgatgttct gatcgatggc acaatgtata ccgttccgat tagtacagaa gagccgtccg tcgtggcggc cttggcccac gccgctaaga ttttccgtat aggtaaggga gtacggacac ttgggccaat caatccattc atcctcggcc agcttatttt catgctgatc gatgggcagt ggaagcaccc aatgaatctg tccgaactcg aaagcctcct atcacgatcg ctggcaacac accttcccaa ttcacgtaac tacggcaagg gaatcgtctc ttgctaccta cgaaagctca atgactacat attattagag atagggcttg atcccggaga agctatgggt gcaaacatga ttaatggatt catggaggca atagcaccag atgtagagcg ctatctcacg aagtttggtc atgtggagcg gttgacatcc attctttcta actctggagc tggacgggag tacgaagcaa tcgcaaagat aactgtggaa gattacgcca tggcgaagaa tatccccatg aagaaggccg aagacatcct gcgacgtata gaggccctca gccagcttgc agagatggac accaatagaa ggataactca taataagggg gtgctgaacg gtatcattgg ggttgccctc gcatgtggac aagacacacg tgccattgat gttgcgaact gccagctcgg ccacacgaag cccttggtca cccacaggtt caacagcaag gagttgtttc ttcgacttac cgcaagaatc tcggcaccgg tcggggtcat aggaggatca tccaaattat tctggggact ctccattctt ggccaggctc aggagaatcg aattagtagc ggctccctgg gtcgcatttt ggctgccgtc gggcttctgc aaaactttgc tgctttacaa gctctagtga caaatgggat acaacacggc catatgaagc ttcataatag gaaactagtg acacgagaaa cgccaacgcc ggcatcagca tcaagctcta tttcagcctc attgcaggcc gttagcatag aacctaaaac tactccaccc aaggtgacca ctccattcat ggacagtcaa gatagagata aaaacaaatt gaatacagcg ctgaatgagt ccctcaagag gatagaggac attatttctc acatcaacta cacgccagaa gagcctaact acgaagccaa gatgatgtta actaatgggg aatcagatct gtacaggtac ttttctcccg ctaagctgag ccctgtgagg aacctccagc aaccgtcatt gcgaatgcaa agtccgaaac ggcaatcgta tgcattagaa aagttagcct cccagacact gttagatagg gtgcgcaccc ggtccccaat cctttctcaa tctatcagct ctgacagccc atttggcgcc gctagcactc atcaacctac agttctctca acactagcaa aaggattagg tcagtctgcg tccacttata acggtggcca atctgcagac ttgttcggtt caggacacgg taagccggcg tcttcatcta cactcaccag gcagatgtcc tacagcccaa acagactcaa tagctttagt gtagtcagtg accgcacaac gtcgccggta aagagcctgg agatggagca ctgggaaggg aactccgggg cgagaaatga acttagcacc agcaagaggc cctacaatat agtctctccg agtaatttaa agcataattc ttggagaaac tcttag
Details for GilaA.19525.a.B1.GE41342
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 83kDa
OBSERVED MW: 83kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMD ISLDYELKTG FAVETVMRTL QYPEERINWL EEHKAINSEL GDELRAFAAV IGLDGPAQMY GFTNDARVRS ASITSPIRSA YAEMRENSGK IKEIVCENNA KRPSSPSLVV TDPLTNALQI ALAQAKVRSD HEVSKYSRRI QALDTMSENV IGATMLPLSV VPDVLIDGTM YTVPISTEEP SVVAALAHAA KIFRIGKGVR TLGPINPFIL GQLIFMLIDG QWKHPMNLSE LESLLSRSLA THLPNSRNYG KGIVSCYLRK LNDYILLEIG LDPGEAMGAN MINGFMEAIA PDVERYLTKF GHVERLTSIL SNSGAGREYE AIAKITVEDY AMAKNIPMKK AEDILRRIEA LSQLAEMDTN RRITHNKGVL NGIIGVALAC GQDTRAIDVA NCQLGHTKPL VTHRFNSKEL FLRLTARISA PVGVIGGSSK LFWGLSILGQ AQENRISSGS LGRILAAVGL LQNFAALQAL VTNGIQHGHM KLHNRKLVTR ETPTPASASS SISASLQAVS IEPKTTPPKV TTPFMDSQDR DKNKLNTALN ESLKRIEDII SHINYTPEEP NYEAKMMLTN GESDLYRYFS PAKLSPVRNL QQPSLRMQSP KRQSYALEKL ASQTLLDRVR TRSPILSQSI SSDSPFGAAS THQPTVLSTL AKGLGQSAST YNGGQSADLF GSGHGKPASS STLTRQMSYS PNRLNSFSVV SDRTTSPVKS LEMEHWEGNS GARNELSTSK RPYNIVSPSN LKHNSWRNS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca tctcccttga ttacgaacta aaaacaggtt ttgcggttga aaccgtaatg cgcactctac aatatccaga agagcggata aattggcttg aggagcacaa agccatcaat agtgagttgg gagatgagct tcgcgcattc gcagcggtta tcggattgga tggaccagcg caaatgtacg gttttacgaa cgacgcccgc gttcgtagcg cgtccataac aagcccaatc cgcagtgcat acgcagaaat gcgggaaaac agtggcaaaa ttaaggagat agtttgcgag aataatgcaa aacggccatc aagtccaagc ttggtagtga cagacccatt gacaaatgct cttcagatcg cgcttgcaca agcaaaagtc agaagcgacc acgaagtctc caagtactcg cggagaatcc aggctcttga tacaatgagc gaaaacgtta ttggagcgac aatgcttcca ttgtccgtcg ttcccgatgt tctgatcgat ggcacaatgt ataccgttcc gattagtaca gaagagccgt ccgtcgtggc ggccttggcc cacgccgcta agattttccg tataggtaag ggagtacgga cacttgggcc aatcaatcca ttcatcctcg gccagcttat tttcatgctg atcgatgggc agtggaagca cccaatgaat ctgtccgaac tcgaaagcct cctatcacga tcgctggcaa cacaccttcc caattcacgt aactacggca agggaatcgt ctcttgctac ctacgaaagc tcaatgacta catattatta gagatagggc ttgatcccgg agaagctatg ggtgcaaaca tgattaatgg attcatggag gcaatagcac cagatgtaga gcgctatctc acgaagtttg gtcatgtgga gcggttgaca tccattcttt ctaactctgg agctggacgg gagtacgaag caatcgcaaa gataactgtg gaagattacg ccatggcgaa gaatatcccc atgaagaagg ccgaagacat cctgcgacgt atagaggccc tcagccagct tgcagagatg gacaccaata gaaggataac tcataataag ggggtgctga acggtatcat tggggttgcc ctcgcatgtg gacaagacac acgtgccatt gatgttgcga actgccagct cggccacacg aagcccttgg tcacccacag gttcaacagc aaggagttgt ttcttcgact taccgcaaga atctcggcac cggtcggggt cataggagga tcatccaaat tattctgggg actctccatt cttggccagg ctcaggagaa tcgaattagt agcggctccc tgggtcgcat tttggctgcc gtcgggcttc tgcaaaactt tgctgcttta caagctctag tgacaaatgg gatacaacac ggccatatga agcttcataa taggaaacta gtgacacgag aaacgccaac gccggcatca gcatcaagct ctatttcagc ctcattgcag gccgttagca tagaacctaa aactactcca cccaaggtga ccactccatt catggacagt caagatagag ataaaaacaa attgaataca gcgctgaatg agtccctcaa gaggatagag gacattattt ctcacatcaa ctacacgcca gaagagccta actacgaagc caagatgatg ttaactaatg gggaatcaga tctgtacagg tacttttctc ccgctaagct gagccctgtg aggaacctcc agcaaccgtc attgcgaatg caaagtccga aacggcaatc gtatgcatta gaaaagttag cctcccagac actgttagat agggtgcgca cccggtcccc aatcctttct caatctatca gctctgacag cccatttggc gccgctagca ctcatcaacc tacagttctc tcaacactag caaaaggatt aggtcagtct gcgtccactt ataacggtgg ccaatctgca gacttgttcg gttcaggaca cggtaagccg gcgtcttcat ctacactcac caggcagatg tcctacagcc caaacagact caatagcttt agtgtagtca gtgaccgcac aacgtcgccg gtaaagagcc tggagatgga gcactgggaa gggaactccg gggcgagaaa tgaacttagc accagcaaga ggccctacaa tatagtctct ccgagtaatt taaagcataa ttcttggaga aactcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t