GilaA.18706.a

Kinase, ULK

CENTER ID: GilaA.18706.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.18706.a.B2.GE37535 kinase domain 1 263
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_17368
RefSeq: XP_001705384.1
UniProt: A8BQY4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHRYEQIAQI GKGSFGVVYK CRLKQTGQFV AVKQISTYGK SSSELAALNS EISLLKEINH PNTIRFFESF EEDGSVVIVT ELAQSDLHSI FASDGPLNIE TIQKVCFQLL NVLTYLHSRR IAHRDIKSQN VLISQGVCKL CDFGFARQMS QQTLALQSIK GTPLYLAPEI AKGKKYNTIS DMWAFGVMIF ELATGRTPFT ATDFLTLMSI LQDDSNKVPY DKYPIFAREP LFKSFCECML QRNPDKRWAS KQMLDHPFLN EGSAIRLAKH VNDAYKFKDW LALLRTMKQQ SALQSILLTV NTEAVVEVVE QGALTEEYAD NFYDNRLIDA INEAINSAHD LPPIVIALMA LLSINSNQAE AIFTSEVVSS HIHNADSRNL EKDYYRILAY IHSHPGDKEE AFYRVADFMA FLIVRSSSTF LQLIQSALRC ISKTNLAQTD VISIVLLLRV SAVCFGCMFT WNVPISFGQI VSFPYDMYIK AEDVVFPSKA EDKPVLTRRA HIVAAAINTL SATSTIEKLF ESFNTIISKS IGVIETSTES FIIKCLLYCI NVSFCMGTTA QSSVQFLMQE YVLYLPSGNR MIGLEHLLTD DYADADMGSG GDEVKKGSGN DGFKLRTIDV RYDVNIRNSL FGQIDTRDYN KFRFLPEYFK KFTSSEKATV ISTDLFGEDE YSYVGIFLSE NFKAYINRFV DLISGRCSRD YPILLTRLYV IFKRLINFKN GIKLDEGYIT IYNNYTTTCL QTLFQAADAV SQTDPNNAMQ AAIATIIKNL NLPILSADSS SSGVLKYTMP SLGQTIGKTA VGDVLSGQTK INPSTPREGK GASGDGYAHG LRSNFDMLRA GSMTVSLAAT SMAGMNQSIN PHTLTPVSAI YPTVEFALIL LHLRQTLLQN HQAIINMFTV RMPAKMLSTG KLSALYAYYR EPYCFNLEVS KGSNMAGITV ETYHGSTAVE LNNMVSGRSK TEMSILLNSE NTFLIEYLNT FKKQLAMCYC FLPFAYIQSD EKEVFRQLNS SREEFRRIVA EKEFVETQLS KKFHRGSVIE MQNKLQTDAE TVDSIFTSFV RTVLAEIQSL KLNSFRFLKH IFAPSFQYLF PLYTYSRKDP SPLAHHALYL KRRFQQSGIP NGDDINLNDI RDNDIKTLVK LLIQADLPRR GHFIITIPQV CYIFDGMSLP GNPFSIQISS DPFSTFSLYQ TNANLVSTYM ILSECIRMVV EEAFLNGLLV LTTENDVQMY QISNVVVQKY IDIHVIFATS TDGTIPPSDY LYQGLPVCAY VNVKQGEGGT DKILIDKNAQ LDYLDKEDVR VNALLTLYNC AFCSEDYARN LSQITFPISD VITENKDETK LSVITMLAIS CLWLQAHGEE DDLRLSLFGL LLTSNFEYNM NCLQRDSEYL VKLYKPDSMS TIKDLVLLSL VRRVSVFAGS VEESLAEARL IMNGSSYSRV DDVYTKAQDD AVISSNPQDF NILSHGALRV ERIMRELIKA GGIIDYGHSD FDGGFDRYNY PKLTDVHIDF NVRQLRRSYY YLSTGTAIEV PSINNYLELE VPVPVFYACI DAITPILKHC KDISIKQPHN STVYSVLASE SREGVIADCI GISRAIFASL DYVLVEPSFR AFLMPCHNYV GFLDSALNYI NILAQEHIDV LPLFVKLSVY LNLSLYNSIE AAFCAAYAAP HHGAILGEEE STATSVSSTV SLGTPLLSAR PAEMKTPILT PSQSLKLTSR PPSYGAHTPN ANEHDLPEVT TSRSTARGKT PLKLGSYSTK PAVLYEQKSE TGYPIISTYL GPGSTHYNGI LMCSLINNCS DCMADCLGLD TRRYDLGGLE GRNQEISNAN WHWRFLASPQ GLIKYIISVR NTLSLLANKR VSGLTDPPGD VNYNSAGWTA ISSIDEFNQY MSYPLPEVVR TLCAAISPQS LDNIILCPYQ FGGGQRAAET LMRYALLAIY QIVYLSCRPS TEKIGDRMEE LKRTTNTTKK DKHTDTARLV YDPDRYYMTE YVSSVLLEFN FIKHVIDSLY YLRSFHTLYY ALVTMVFFAM HPQDSIVIPW INQFIFSGGV SMNLLSAIFS QKNISQRNNP HSCILEILIL LLRAVQVSQE YAELVSNTIS ASTIIQFLSF ESSEELIVKS CEFVGYLIKA RPTASEQYKT CLDSLIELIK TRRTAIVTKS LFAMMHIAKT VDDAFSLSKC IGCICKTLMN ARVLDDIKTC AAVASAKIIL AHEELATEFV SGEGVISLVD CMSSCDQLSD KMIYAKVLLE LAKFDPVTTA LKSQASLLRM IDRDCDDHAL KSLLENLLIT SKRM
NT Sequence
atgcatcgct acgaacagat tgcccagata gggaagggct catttggcgt tgtatacaag tgtcgcctca agcagacagg gcagtttgta gccgttaagc agatatcaac ctatggcaag tcatcctccg aacttgcagc gctcaactca gagatatcct tactgaagga aatcaatcac cccaatacaa ttcggttttt tgaatccttt gaagaggatg gcagcgttgt catcgtcacc gagttggctc agtccgacct tcacagtatc tttgccagcg acgggccgct taatattgag actattcaaa aggtgtgctt tcagctgctt aatgttttaa catacctcca ttcccgccgg attgcacaca gagacatcaa gtcacagaac gttttgatat ctcagggtgt ctgtaagctt tgtgacttcg gatttgcgcg ccagatgtca cagcagaccc ttgctctcca gtctatcaag gggactcctc tctacttagc ccccgaaatt gccaaaggaa agaagtataa tacaatctct gatatgtggg catttggcgt gatgattttt gagttggcca cgggcaggac cccgttcact gctactgact ttcttactct catgagcatc ctccaggacg actccaacaa ggtgccgtat gacaagtatc ctatctttgc gcgagagcct cttttcaaga gcttttgcga gtgcatgctc cagcgtaacc ccgataagcg ctgggcctcc aagcagatgc tagatcatcc gtttctcaac gagggctctg ctatacgatt ggctaagcat gttaatgacg cttacaagtt taaagattgg ctcgctttgc tccggacaat gaaacagcaa agtgctcttc aatccatcct tcttacggtt aacacagagg ctgtggttga ggttgtcgaa cagggtgccc tcacagaaga gtatgcggat aatttctatg acaataggct catcgatgcg atcaacgagg cgattaattc tgctcatgac cttccaccca ttgtgatagc cttaatggcc cttttatcca tcaattctaa tcaggcagag gccatcttca cgtcagaggt tgtttcctcg cacattcaca acgccgactc acggaatctt gaaaaagact attaccgcat actggcctac attcattcgc accccggaga taaggaagag gcattctacc gtgtggcaga tttcatggca ttccttattg taagatcctc cagcactttc ctccaactga tacagtcagc tctgcgctgt atctccaaga caaatcttgc acaaacagat gtcatttcaa tcgttcttct tttgcgggtt tctgctgtat gcttcggctg tatgttcacg tggaatgttc cgataagctt tggtcaaata gtgtccttcc cttacgacat gtacattaag gcagaggatg ttgtcttccc ctctaaggcg gaggacaagc ctgtgcttac caggagggca cacatagtgg ctgcagccat caacacttta tctgccacaa gcacgattga aaagctattt gagtccttca acacaatcat ctccaaaagc attggtgtta ttgagacgtc aactgagtcg tttataatca agtgccttct ttactgtatc aatgtctctt tttgcatggg gaccactgct caatcaagtg ttcagttctt aatgcaggag tatgttctct atctcccttc gggaaatcgc atgataggtt tagaacacct actcacagat gactatgccg atgctgatat gggctcgggt ggcgacgaag tgaagaaggg gtctgggaac gacgggttca agctcaggac aatagatgtg cgctacgatg ttaatattag aaacagcctc ttcggtcaga tagatacacg tgactacaac aaattcaggt ttttgccgga gtattttaaa aagtttacta gttctgagaa ggccacagtg attagcacag atttatttgg ggaagatgag tatagctatg ttgggatttt tctcagcgaa aactttaagg cgtatattaa tcgattcgtt gatcttattt ccggacgctg ctcccgtgac tatcccatat tgctcactcg tctctacgtt atcttcaagc ggctcatcaa tttcaaaaat ggcatcaagc tggatgaagg ctacatcaca atatataaca attacacaac cacctgttta caaactctct ttcaagcagc agatgcggtt tctcaaacag accccaacaa cgctatgcaa gcagctatag cgacaataat aaaaaacctc aacctcccta ttctttcggc agattcgtca tcgtcaggcg tcctcaagta taccatgccc tctctcggtc agacaattgg gaaaacagcc gtaggagatg tgctgtctgg ccaaaccaag atcaacccct ccaccccgcg cgaaggcaag ggagccagtg gtgacggtta tgcgcatggt ctcagatcca attttgatat gctccgtgcc ggaagtatga cagtgagtct agcagcgacc agcatggctg gaatgaatca gtctataaat ccacatacat tgacgcccgt ctctgctatt tacccaaccg tagaattcgc gctcattctt cttcatttgc gccaaacact tttgcagaac catcaggcta ttattaacat gtttacagtc aggatgcctg caaaaatgct ttctacagga aaactatctg ctctgtatgc ctattacaga gagccgtact gttttaatct ggaggtctcg aaagggtcga acatggccgg aatcactgtg gagacatacc acggctcgac agctgtggag cttaataaca tggtgagtgg gcggagcaag accgagatgt ctatcttgct gaactctgag aacacttttt tgatagagta ccttaacacc tttaagaagc aactagctat gtgctactgc ttccttccct ttgcgtacat acagagtgat gagaaagaag tgtttaggca actgaattcg tctcgggaag agttccgccg gattgtagca gaaaaagagt ttgtagaaac gcagctatcc aagaaatttc atcgcggttc cgtgattgaa atgcaaaaca agctacagac cgatgcagag acggtggact ctatttttac tagctttgta aggactgtct tggcggaaat acagtctctc aaactgaact ccttccggtt cttaaagcat atctttgcgc cgtcctttca atacttgttc ccgctgtaca catattcccg caaggacccg tctcccctgg cccaccatgc tctatactta aagcgacggt tccagcagtc cggtatacct aacggagatg atatcaacct gaacgacata agggataacg atattaagac tttagtgaag ctcctcatcc aggcagacct tcctagaagg ggtcatttta tcatcacaat tcctcaggtg tgctatatct ttgatggtat gagccttcca ggaaacccat tctccatcca gatctctagc gatccctttt ctacgttttc tctctaccag accaatgcca atctcgtttc aacctatatg atcctttctg agtgcatccg aatggtcgtg gaggaggcct tcttgaacgg tcttctggta ctgaccacag agaatgatgt gcagatgtac cagatttcaa acgtagttgt ccagaaatat atcgacatcc acgttatctt tgctacatca acggatggca ccataccccc atcggactat ttatatcagg gccttccggt ttgcgcctat gtgaatgtga agcagggtga aggtggaaca gataaaatcc ttattgataa aaatgcgcaa ctggactatt tagataaaga agatgtgcgg gtcaatgccc ttttgaccct ctataactgt gcgttttgca gtgaagatta cgctcggaac ctcagtcaga taacgtttcc gatctcggac gttatcacgg agaataaaga cgaaacaaag cttagcgtta ttacgatgtt ggcaatatcg tgtctttggc tccaagccca cggtgaggag gacgatcttc gcttgagcct atttggcttg cttctaactt ccaattttga gtataacatg aactgtttgc agagggacag tgaatacctc gtcaagctct ataagccaga tagcatgtcc accattaaag acttggttct gttgtcgcta gtaagacgcg tgagtgtctt cgcaggtagc gttgaggaat cgctggcaga agcgcgactg attatgaacg gaagtagtta cagccgtgtt gatgatgtat acacaaaagc tcaagacgac gccgttatta gctccaatcc tcaagacttt aatatcttgt ctcatggcgc tctccgcgtt gagcgcatca tgagggagtt aattaaggca ggcggaatca tcgactatgg acactcggac tttgatggag gattcgatag atacaactac cccaaactaa ccgatgttca catagacttt aacgtcaggc aattacgccg ctcgtactat tatttatcca ctggaacagc aatagaagtt ccttctatta ataactattt agagttggaa gtccccgttc cagtattcta tgcgtgcatt gatgctatta ccccaatcct caagcattgc aaggacatct caataaaaca gccgcacaat tcaacagttt attccgttct tgcctcggaa tctagggaag gagtaatagc agattgcatt ggtatctcgc gtgccatatt tgctagcctg gattatgtgc ttgtggagcc gagctttcgg gcgtttctaa tgccatgtca taactacgtg gggttcttgg acagcgcact taactacatt aatatattag cgcaggaaca catcgacgtc ttgcctctct ttgtgaagct atcagtttat ttgaacctct ctctatacaa ctctatagaa gctgcctttt gtgccgcata tgctgcgccc caccacggtg caattttggg cgaggaagaa tcgactgcca ccagcgtctc ctctaccgtc tctttgggaa caccgttgtt atctgcacga ccagcagaaa tgaagactcc catcttgact cctagtcaat cattgaagct gacaagtcgt ccgccgagct atggagccca tacacccaat gctaatgaac atgatcttcc ggaggttact actagtcgta gtaccgcacg cggtaagaca ccgctcaagt taggaagcta ctcaacaaaa ccagcagttc tatatgaaca gaagtctgag acaggatacc caatcatatc tacttactta gggcctggat cgacgcatta caatgggatt cttatgtgca gtttaatcaa caactgcagc gattgcatgg cggattgcct tgggttagat acccggaggt atgacttagg agggcttgag ggccgaaatc aggaaatatc caacgctaat tggcactggc ggtttcttgc atccccacag ggcctcatca agtacattat ctcagtacgg aacacgcttt ctttattggc caacaaacgc gtatctggtc ttactgaccc tccaggcgac gtcaactata actcggctgg gtggacggct ataagctcca tcgatgagtt caatcagtac atgtcctacc ccttgccaga agttgtgagg accttgtgtg cagcgatctc tccacaatca ctcgacaaca tcatactctg tccgtatcag tttggaggag ggcaaagagc tgcagagact ctgatgcgat acgcattgtt agccatatac caaatcgtct atttgtcatg ccgtcccagt acagagaaga ttggagacag gatggaggaa ctcaaaagga ctactaacac aactaaaaag gacaagcaca cagacacggc ccgtcttgtc tatgatccag atcgctatta catgacagag tacgtctcat ctgtgttgct ggagttcaac ttcatcaagc acgttataga ctcattgtat taccttagga gcttccacac actgtattat gcgcttgtga cgatggtctt cttcgcaatg cacccccaag actcgatagt gataccgtgg attaaccaat ttatcttcag tggaggcgtt tcaatgaacc ttttgtctgc aatattttct cagaagaaca taagtcagcg gaacaatcct cactcgtgta tccttgaaat tctaattcta cttcttagag ctgtgcaagt ctcacaagag tacgccgagc tagtaagcaa cactatttct gccagcacca tcattcagtt tctttccttt gagtcatcag aagagcttat tgtgaagtcc tgcgaatttg ttggttattt aataaaggcg cgacccacgg cttctgaaca atacaaaaca tgcttagaca gcctaataga gcttattaag acgcggagga cagctatagt tactaagtct ctctttgcaa tgatgcatat tgccaagaca gttgatgatg catttagcct cagtaagtgc attggctgca tctgcaagac tcttatgaac gctcgcgttc tagacgacat caagacctgc gcggccgtag cgtctgccaa gattattcta gcccatgaag agttagctac ggagtttgtt tcaggagaag gcgtcattag ccttgttgac tgcatgtcta gttgcgacca gctctcggac aagatgatat acgctaaagt ccttctggaa ctggcgaagt ttgacccggt aaccacagcc ctcaaatctc aggcgtcact attgcggatg atagaccgcg attgtgatga tcatgccctc aagtctctgc ttgaaaactt gctgatcaca agtaaaagaa tgtaa
Details for GilaA.18706.a.B2.GE37535
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/6/2013
EXPECTED MW: 31kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MHRYEQIAQI GKGSFGVVYK CRLKQTGQFV AVKQISTYGK SSSELAALNS EISLLKEINH PNTIRFFESF EEDGSVVIVT ELAQSDLHSI FASDGPLNIE TIQKVCFQLL NVLTYLHSRR IAHRDIKSQN VLISQGVCKL CDFGFARQMS QQTLALQSIK GTPLYLAPEI AKGKKYNTIS DMWAFGVMIF ELATGRTPFT ATDFLTLMSI LQDDSXKVPY DKYPIFAREP LFKSFCECML QRNPXKRWAS XQMLDHPFLN EGS
Validated NT Sequence
ggctcaccac caccaccacc atatgcatcg ctacgaacag attgcccaga tagggaaggg ctcatttggc gttgtataca agtgtcgcct caagcagaca gggcagtttg tagccgttaa gcagatatca acctatggca agtcatcctc cgaacttgca gcgctcaact cagagatatc cttactgaag gaaatcaatc accccaatac aattcggttt tttgaatcct ttgaagagga tggcagcgtt gtcatcgtca ccgagttggc tcagtccgac cttcacagta tctttgccag cgacgggccg cttaatattg agactattca aaaggtgtgc tttcagctgc ttaatgtttt aacatacctc cattcccgcc ggattgcaca cagagacatc aagtcacaga acgttttgat atctcagggt gtctgtaagc tttgtgactt cggatttgcg cgccagatgt cacagcagac ccttgctctc cagtctatca aggggactcc tctctactta gcccccgaaa ttgccaaagg aaagaagtat aatacaatct ctgatatgtg ggcatttggc gtgatgattt ttgagttggc cacgggcagg accccgttca ctgctactga ctttcttact ctcatgagca tcctccagga cgactccnac aaggtgccgt atgacaagta tcctatcttt gcgcgagagc ctcttttcaa gagcttttgc gagtgcatgc tccagcgtaa ccccnataag cgctgggcct ccnagcagat gctagatcat ccgtttctca acgagggctc ttaagtgagt aaganaggan ccggctgcna ca
Expected Protein Sequence
MAHHHHHHMH RYEQIAQIGK GSFGVVYKCR LKQTGQFVAV KQISTYGKSS SELAALNSEI SLLKEINHPN TIRFFESFEE DGSVVIVTEL AQSDLHSIFA SDGPLNIETI QKVCFQLLNV LTYLHSRRIA HRDIKSQNVL ISQGVCKLCD FGFARQMSQQ TLALQSIKGT PLYLAPEIAK GKKYNTISDM WAFGVMIFEL ATGRTPFTAT DFLTLMSILQ DDSNKVPYDK YPIFAREPLF KSFCECMLQR NPDKRWASKQ MLDHPFLNEG S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatc gctacgaaca gattgcccag atagggaagg gctcatttgg cgttgtatac aagtgtcgcc tcaagcagac agggcagttt gtagccgtta agcagatatc aacctatggc aagtcatcct ccgaacttgc agcgctcaac tcagagatat ccttactgaa ggaaatcaat caccccaata caattcggtt ttttgaatcc tttgaagagg atggcagcgt tgtcatcgtc accgagttgg ctcagtccga ccttcacagt atctttgcca gcgacgggcc gcttaatatt gagactattc aaaaggtgtg ctttcagctg cttaatgttt taacatacct ccattcccgc cggattgcac acagagacat caagtcacag aacgttttga tatctcaggg tgtctgtaag ctttgtgact tcggatttgc gcgccagatg tcacagcaga cccttgctct ccagtctatc aaggggactc ctctctactt agcccccgaa attgccaaag gaaagaagta taatacaatc tctgatatgt gggcatttgg cgtgatgatt tttgagttgg ccacgggcag gaccccgttc actgctactg actttcttac tctcatgagc atcctccagg acgactccaa caaggtgccg tatgacaagt atcctatctt tgcgcgagag cctcttttca agagcttttg cgagtgcatg ctccagcgta accccgataa gcgctgggcc tccaagcaga tgctagatca tccgtttctc aacgagggct cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat