GilaA.18664.a

Kinase, NEK

CENTER ID: GilaA.18664.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.18664.a.B2.GE37533 kinase domain 1 312
GilaA.18664.a.B4.GE37534 soluble domain 1 1 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_13215
RefSeq: XP_001710065.1
UniProt: A8B2W4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLYQYQKIAW KKRPTLRRNL KKSKRLRPEH NLKVNIIIKM VAPGVPAGYE QLAVLGNGAF GKVYKVRRQS DGVIMALKII DLSALSSNAQ EVLRNETELF SLLEHRNLVK CYETFQNAAA GEQYVAMELC ERSLKDLILE CRRSQKHLPE KTIWLYAAQI LQGVSYLHSR YKPNDTRLKC IIHRDLKPDN ILLTGDSMIK ICDFGISRPL GVVSKAVTLA GTYHYMAPEI IKGQKYTEKV DVWSIGCVVY ELCTLKVPTF DTLFSSGQPS MEFISFTLPD MYSTELKKFI EWCLIIRQDD RLSVYELLSY PPIKAIIAES AIAPPTKPAV NAPVENKHKM FETDEVNAPS KPGNAAKDPG PARSPAAPAP APAKNASSTP VLLSGFTEEQ PRAITRQVKK ECYFDKNNNT NLMLMVLSGA SVETIRANLH LCKFINKDGE TALHMAVDKQ ARAYVELLAP REATIRLREA VFSSVRNFPI KSKKLYNVSA MMLAALYGMS DIVDVLRPYE SGLQSAEGYT ALMLAILAGS TTSATLLLHE ATRQTQRGET ALLFAAKVGN KDLVDRLIPY EAKVVTNYGS TALMEACRAG NMSIVKQLLP HEGRLVVYDM NVNLPHITKG FANKQEIKQL LIRLG
NT Sequence
atgttgtacc agtatcagaa gatagcctgg aagaaaagac caactctaag aagaaatcta aaaaagtcaa aaagattaag acctgagcac aatttaaaag taaacatcat cataaaaatg gtcgccccgg gcgtccccgc tggctatgag cagctggccg tgctgggtaa tggggcattc gggaaggtgt acaaggtaag gaggcagtct gacggggtta taatggctct taaaataatc gacctgagcg cactgtcgtc aaatgcgcaa gaggttttgc gcaatgagac agagcttttc tcactattag agcatcgcaa cttggtcaag tgttacgaga ccttccaaaa cgcagcggct ggtgaacagt acgtagccat ggagctttgt gagaggtctc tgaaggacct catactggag tgtcggcgct cacagaaaca ccttccagag aagactatct ggctctacgc agcacagatc ctccagggtg tctcttacct ccactccagg tacaagccga atgacacaag actcaagtgc atcattcatc gtgatttgaa gcctgacaac attctgctca ccggcgactc catgataaag atatgcgact ttgggatttc gaggccactg ggagttgttt ctaaggcagt gacactagcg ggcacctacc attacatggc gcccgaaata atcaagggcc aaaagtacac ggagaaggtg gatgtctggt caattggatg cgtggtttat gagctctgca cactcaaggt gcctactttt gacacccttt tttcttctgg gcagccatca atggagttca tctcttttac cttgcctgac atgtacagta ctgagctgaa gaaattcatt gagtggtgcc tcataatacg gcaagacgat agactttctg tgtatgagtt acttagctac ccgccgataa aggccattat cgccgaaagt gcgattgctc ctccgaccaa gcctgccgta aatgctcctg tagagaataa gcacaagatg tttgagaccg atgaggtcaa tgcaccctct aaaccgggga atgctgcgaa ggaccctggc cctgcccgct ctccggctgc ccctgctccc gctcctgcga aaaacgcctc atccactcct gttctcctct cgggattcac cgaagaacag cccagggcta ttacacgaca ggttaagaag gagtgctatt ttgataagaa caataacacg aatctaatgc tgatggtgct atctggtgca tccgttgaga ctatcagggc caatttgcac ctatgcaagt ttattaataa agatggcgaa acggcgctgc acatggctgt tgataaacaa gcgcgtgcat acgttgagct actggcccca agggaggcca ctatacgact gcgagaagct gtcttctcgt ctgttcgcaa ctttccaatc aagtcgaaga agctgtacaa tgtgtcggct atgatgctcg ctgctctgta cggaatgtca gacatagttg atgtgctccg gccatacgag tctgggcttc aaagtgctga aggatatacc gctctgatgt tggctatcct tgctgggtca acgacttcag cgactctcct cctccatgag gccactcgcc aaacgcagcg cggtgaaaca gccttacttt tcgccgccaa agtgggaaac aaagaccttg tagataggct aataccctat gaggctaaag tagtgactaa ctatgggtcc actgctttga tggaagcgtg tagagcaggg aacatgtcca ttgtcaagca gctccttcct cacgaaggcc gtctagttgt atatgatatg aacgtcaatc ttccacacat caccaagggt ttcgccaaca agcaggaaat caagcagtta cttatccggc tcggctaa
Details for GilaA.18664.a.B2.GE37533
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MLYQYQKIAW KKRPTLRRNL KKSKRLRPEH NLKVNIIIKM VAPGVPAGYE QLAVLGNGAF GKVYKVRRQS DGVIMALKII DLSALSSNAQ EVLRNETELF SLLEHRNLVK CYETFQNAAA GEQYVAMELC ERSLKDLILE CRRSQKHLPE KTIWLYAAQI LQGVSYLHSR YKPNDTRLKC IIHRDLKPDN ILLTGDSMIK ICDFGISRPL GVVSKAVTLA GTYHYMAPEI IKGQKYTEXV DVWSIGCVVY ELCTLKVPTF DTLFSSGQPS MEFISFTLPD MYSTELKKFI EWCLIIRQDD RLSVYELLSY PP
Validated NT Sequence
ccnnnnnnnn ananttnnnn ttantttaan aaaggaatat ataccagggc tcaccaccac caccaccata tgttgtacca gtatcagaag atagcctgga agaaaagacc aactctaaga agaaatctaa aaaagtcaaa aagattaaga cctgagcaca atttaaaagt aaacatcatc ataaaaatgg tcgccccggg cgtccccgct ggctatgagc agctggccgt gctgggtaat ggggcattcg ggaaggtgta caaggtaagg aggcagtctg acggggttat aatggctctt aaaataatcg acctgagcgc actgtcgtca aatgcgcaag aggttttgcg caatgagaca gagcttttct cactattaga gcatcgcaac ttggtcaagt gttacgagac cttccaaaac gcagcggctg gtgaacagta cgtagccatg gagctttgtg agaggtctct gaaggacctc atactggagt gtcggcgctc acagaaacac cttccagaga agactatctg gctctacgca gcacagatcc tccagggtgt ctcttacctc cactccaggt acaagccgaa tgacacaaga ctcaagtgca tcattcatcg tgatttgaag cctgacaaca ttctgctcac cggcgactcc atgataaaga tatgcgactt tgggatttcg aggccactgg gagttgtttc taaggcagtg acactagcgg gcacctacca ttacatggcg cccgaaataa tcaagggcca aaagtacacg gagaangtgg atgtctggtc aattggatgc gtggtttatg agctctgcac actcaaggtg cctacttttg acaccctttt ttcttctggg cagccatcaa tggagttcat ctcttttacc ttgcctgaca tgtacagtac tgagctgaag aaattcattg agtggtgcct cataatacgg caagacgata gactttctgt gtatgagtta cttagctacc cgccgtaagt gagtanatag gatcngctnn nnnaannccc ccnanannnn nnnnnnnnnn nnnnnnaann nnnnntnnng nnntnnnaaa nnnnnnntnn nnacgaacan nnncnanatn ggnnctntga actngtccgn nntcnnntnc nnacaactg
Expected Protein Sequence
MAHHHHHHML YQYQKIAWKK RPTLRRNLKK SKRLRPEHNL KVNIIIKMVA PGVPAGYEQL AVLGNGAFGK VYKVRRQSDG VIMALKIIDL SALSSNAQEV LRNETELFSL LEHRNLVKCY ETFQNAAAGE QYVAMELCER SLKDLILECR RSQKHLPEKT IWLYAAQILQ GVSYLHSRYK PNDTRLKCII HRDLKPDNIL LTGDSMIKIC DFGISRPLGV VSKAVTLAGT YHYMAPEIIK GQKYTEKVDV WSIGCVVYEL CTLKVPTFDT LFSSGQPSME FISFTLPDMY STELKKFIEW CLIIRQDDRL SVYELLSYPP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgt accagtatca gaagatagcc tggaagaaaa gaccaactct aagaagaaat ctaaaaaagt caaaaagatt aagacctgag cacaatttaa aagtaaacat catcataaaa atggtcgccc cgggcgtccc cgctggctat gagcagctgg ccgtgctggg taatggggca ttcgggaagg tgtacaaggt aaggaggcag tctgacgggg ttataatggc tcttaaaata atcgacctga gcgcactgtc gtcaaatgcg caagaggttt tgcgcaatga gacagagctt ttctcactat tagagcatcg caacttggtc aagtgttacg agaccttcca aaacgcagcg gctggtgaac agtacgtagc catggagctt tgtgagaggt ctctgaagga cctcatactg gagtgtcggc gctcacagaa acaccttcca gagaagacta tctggctcta cgcagcacag atcctccagg gtgtctctta cctccactcc aggtacaagc cgaatgacac aagactcaag tgcatcattc atcgtgattt gaagcctgac aacattctgc tcaccggcga ctccatgata aagatatgcg actttgggat ttcgaggcca ctgggagttg tttctaaggc agtgacacta gcgggcacct accattacat ggcgcccgaa ataatcaagg gccaaaagta cacggagaag gtggatgtct ggtcaattgg atgcgtggtt tatgagctct gcacactcaa ggtgcctact tttgacaccc ttttttcttc tgggcagcca tcaatggagt tcatctcttt taccttgcct gacatgtaca gtactgagct gaagaaattc attgagtggt gcctcataat acggcaagac gatagacttt ctgtgtatga gttacttagc tacccgccgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for GilaA.18664.a.B4.GE37534
HARVESTED ON: data unavailable
SEQUENCED ON: 12/6/2013
EXPECTED MW: 56kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML YQYQKIAWKK RPTLRRNLKK SKRLRPEHNL KVNIIIKMVA PGVPAGYEQL AVLGNGAFGK VYKVRRQSDG VIMALKIIDL SALSSNAQEV LRNETELFSL LEHRNLVKCY ETFQNAAAGE QYVAMELCER SLKDLILECR RSQKHLPEKT IWLYAAQILQ GVSYLHSRYK PNDTRLKCII HRDLKPDNIL LTGDSMIKIC DFGISRPLGV VSKAVTLAGT YHYMAPEIIK GQKYTEKVDV WSIGCVVYEL CTLKVPTFDT LFSSGQPSME FISFTLPDMY STELKKFIEW CLIIRQDDRL SVYELLSYPP IKAIIAESAI APPTKPAVNA PVENKHKMFE TDEVNAPSKP GNAAKDPGPA RSPAAPAPAP AKNASSTPVL LSGFTEEQPR AITRQVKKEC YFDKNNNTNL MLMVLSGASV ETIRANLHLC KFINKDGETA LHMAVDKQAR AYVELLAPRE ATIRLREAVF SSVRNFPIKS KKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgt accagtatca gaagatagcc tggaagaaaa gaccaactct aagaagaaat ctaaaaaagt caaaaagatt aagacctgag cacaatttaa aagtaaacat catcataaaa atggtcgccc cgggcgtccc cgctggctat gagcagctgg ccgtgctggg taatggggca ttcgggaagg tgtacaaggt aaggaggcag tctgacgggg ttataatggc tcttaaaata atcgacctga gcgcactgtc gtcaaatgcg caagaggttt tgcgcaatga gacagagctt ttctcactat tagagcatcg caacttggtc aagtgttacg agaccttcca aaacgcagcg gctggtgaac agtacgtagc catggagctt tgtgagaggt ctctgaagga cctcatactg gagtgtcggc gctcacagaa acaccttcca gagaagacta tctggctcta cgcagcacag atcctccagg gtgtctctta cctccactcc aggtacaagc cgaatgacac aagactcaag tgcatcattc atcgtgattt gaagcctgac aacattctgc tcaccggcga ctccatgata aagatatgcg actttgggat ttcgaggcca ctgggagttg tttctaaggc agtgacacta gcgggcacct accattacat ggcgcccgaa ataatcaagg gccaaaagta cacggagaag gtggatgtct ggtcaattgg atgcgtggtt tatgagctct gcacactcaa ggtgcctact tttgacaccc ttttttcttc tgggcagcca tcaatggagt tcatctcttt taccttgcct gacatgtaca gtactgagct gaagaaattc attgagtggt gcctcataat acggcaagac gatagacttt ctgtgtatga gttacttagc tacccgccga taaaggccat tatcgccgaa agtgcgattg ctcctccgac caagcctgcc gtaaatgctc ctgtagagaa taagcacaag atgtttgaga ccgatgaggt caatgcaccc tctaaaccgg ggaatgctgc gaaggaccct ggccctgccc gctctccggc tgcccctgct cccgctcctg cgaaaaacgc ctcatccact cctgttctcc tctcgggatt caccgaagaa cagcccaggg ctattacacg acaggttaag aaggagtgct attttgataa gaacaataac acgaatctaa tgctgatggt gctatctggt gcatccgttg agactatcag ggccaatttg cacctatgca agtttattaa taaagatggc gaaacggcgc tgcacatggc tgttgataaa caagcgcgtg catacgttga gctactggcc ccaagggagg ccactatacg actgcgagaa gctgtcttct cgtctgttcg caactttcca atcaagtcga agaagctgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat