GilaA.18219.a

Glycylpeptide N-tetradecanoyltransferase

CENTER ID: GilaA.18219.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
GilaA.18219.a.A3.GE44102 truncation XtalPred disorder 30 409
GilaA.18219.a.A6.GE44108 truncation XtalPred disorder 39 409
GilaA.18219.a.AE4.GE44105 truncation XtalPred disorder 33 409
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_5772
UniProt: A8B394

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDHAFWNTQ PVVQELVEAL EDGDMPQDLI SVDPAQHPDT SIPLPEKFVW TAIDPHDDGH MADLYKLLHA YYVEDTSNMF RFAYSKDLLR WWLTSPGCKP EYSLGIRIDD PDSEGHGRLI GYISGVVSDY AYSNVDGGEV FSRPMQSIVF LCLDKKYRSL KLAPLLIQEI TRRSYKNGVF HAIYTSGTSL TAPIQVATYY HRILNPLKLA RIGFCTKPKA ISDKEFAFFY KLPFLPTALS SCFHVCTEAD IPVLYDLYLE YTRRKYKFRQ IYSKEMFTHV VTHRKDSIHT LVYREKEGGQ PDAFITYYII DTAVLAKDIR KQYPNMRNGY IYLYSLRDDC NLSIGQLVLA LMHDMQNNNI DVCTALNVGL NPSFFSTMKF AEGSGKLNYY LFNLKWPKMA LKDIGVVLI
NT Sequence
atgcctgatc acgcattctg gaacactcag ccagtcgtgc aggagttggt tgaggctcta gaggatggcg acatgcccca agatcttatt tctgtggatc ctgcccagca tcccgacact tccatcccac tcccggaaaa gttcgtctgg acggctattg atccccacga tgacggccat atggccgacc tctacaagct tttacacgcg tattacgtcg aagacaccag taacatgttt cgcttcgcgt attctaagga tcttttaaga tggtggttga caagtcctgg ttgcaaacca gagtactctc taggaatacg catagacgac ccagatagcg aaggccacgg aaggctcatt ggttatattt ctggggtcgt gagtgactac gcctactcta acgtggacgg aggcgaggtc ttttctaggc ccatgcagag tattgtattt ctgtgcctcg ataagaagta tcgtagtcta aagttggccc cgctgttaat tcaagagatc acacgccgct cgtacaagaa cggagtcttc catgccatat acacgtctgg aacgtctctg accgctccca ttcaggttgc aacctactac caccgcattc tcaatcccct gaagctggca agaattgggt tttgcacgaa gcctaaagca atcagcgaca aggagtttgc ctttttctac aagctgccct ttcttcccac tgctttgtca tcgtgctttc atgtttgcac tgaagctgat atccctgttc tctatgatct ctatctagag tatactcgca gaaagtacaa gttcaggcag atctactcca aagaaatgtt cacacatgtg gtgacgcaca gaaaggatag catccacaca ctggtgtaca gagagaaaga gggagggcag ccagatgcgt ttatcaccta ctacatcatc gacacggcag tcctggccaa agacatcaga aagcaatatc caaacatgcg taacgggtac atttaccttt attctctgcg cgatgactgc aacctgtcta taggccagct agtcctcgcc cttatgcacg atatgcagaa caacaatatc gatgtctgca ctgctcttaa cgtagggctt aaccccagct tcttctccac catgaagttc gcagaaggga gcggaaagct gaattactat ctgtttaatt tgaaatggcc gaagatggcc ttgaaggaca taggggtcgt gttgatatga
Details for GilaA.18219.a.A3.GE44102
HARVESTED ON: 3/2/2021
SEQUENCED ON: 3/10/2021
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISVDPAQH PDTSIPLPEK FVWTAIDPHD DGHMADLYKL LHAYYVEDTS NMFRFAYSKD LLRWWLTSPG CKPEYSLGIR IDDPDSEGHG RLIGYISGVV SDYAYSNVDG GEVFSRPMQS IVFLCLDKKY RSLKLAPLLI QEITRRSYKN GVFHAIYTSG TSLTAPIQVA TYYHRILNPL KLARIGFCTK PKAISDKEFA FFYKLPFLPT ALSSCFHVCT EADIPVLYDL YLEYTRRKYK XRQIYSKEMF THVVTHRKDS IHXLXYXEKX GXQPDAFITY YXIDTAVLAK XIRKXXSKHA YGTFTXILXX DXXQPXYXAS XVXRPFXX
Validated NT Sequence
atacgactca ctatagggag accacaacgg tttccctcta gaaataattt tgtttaactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgatttctg tggatcctgc ccagcatccc gacacttcca tcccactccc ggaaaagttc gtctggacgg ctattgatcc ccacgatgac ggccatatgg ccgacctcta caagctttta cacgcgtatt acgtcgaaga caccagtaac atgtttcgct tcgcgtattc taaggatctt ttaagatggt ggttgacaag tcctggttgc aaaccagagt actctctagg aatacgcata gacgacccag atagcgaagg ccacggaagg ctcattggtt atatttctgg ggtcgtgagt gactacgcct actctaacgt ggacggaggc gaggtctttt ctaggcccat gcagagtatt gtatttctgt gcctcgataa gaagtatcgt agtctaaagt tggccccgct gttaattcaa gagatcacac gccgctcgta caagaacgga gtcttccatg ccatatacac gtctggaacg tctctgaccg ctcccattca ggttgcaacc tactaccacc gcattctcaa tcccctgaag ctggcaagaa ttgggttttg cacgaagcct aaagcaatca gcgacaagga gtttgccttt ttctacaagc tgccctttct tcccactgct ttgtcatcgt gctttcatgt ttgcactgaa gctgatatcc ctgttctcta tgatctctat ctagagtata ctcgcagaaa gtacaagntc aggcagatct actccaaaga aatgttcaca catgtggtga cgcacagaaa ggatagcatc cacncactgg ngtacngaga gaaagangga nggcagccag atgcgtttat cacctactac ntcatcgaca cngcagtcct ggccaaagan atcagaaagc annnntccaa acatgcgtac ggnacnttta cnnttattct nnnnnncgat gnantgcaac ctngctatnn ggccagcttn gtccnncgcc cctttatngn nnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SISVDPAQHP DTSIPLPEKF VWTAIDPHDD GHMADLYKLL HAYYVEDTSN MFRFAYSKDL LRWWLTSPGC KPEYSLGIRI DDPDSEGHGR LIGYISGVVS DYAYSNVDGG EVFSRPMQSI VFLCLDKKYR SLKLAPLLIQ EITRRSYKNG VFHAIYTSGT SLTAPIQVAT YYHRILNPLK LARIGFCTKP KAISDKEFAF FYKLPFLPTA LSSCFHVCTE ADIPVLYDLY LEYTRRKYKF RQIYSKEMFT HVVTHRKDSI HTLVYREKEG GQPDAFITYY IIDTAVLAKD IRKQYPNMRN GYIYLYSLRD DCNLSIGQLV LALMHDMQNN NIDVCTALNV GLNPSFFSTM KFAEGSGKLN YYLFNLKWPK MALKDIGVVL I
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ttctgtggat cctgcccagc atcccgacac ttccatccca ctcccggaaa agttcgtctg gacggctatt gatccccacg atgacggcca tatggccgac ctctacaagc ttttacacgc gtattacgtc gaagacacca gtaacatgtt tcgcttcgcg tattctaagg atcttttaag atggtggttg acaagtcctg gttgcaaacc agagtactct ctaggaatac gcatagacga cccagatagc gaaggccacg gaaggctcat tggttatatt tctggggtcg tgagtgacta cgcctactct aacgtggacg gaggcgaggt cttttctagg cccatgcaga gtattgtatt tctgtgcctc gataagaagt atcgtagtct aaagttggcc ccgctgttaa ttcaagagat cacacgccgc tcgtacaaga acggagtctt ccatgccata tacacgtctg gaacgtctct gaccgctccc attcaggttg caacctacta ccaccgcatt ctcaatcccc tgaagctggc aagaattggg ttttgcacga agcctaaagc aatcagcgac aaggagtttg cctttttcta caagctgccc tttcttccca ctgctttgtc atcgtgcttt catgtttgca ctgaagctga tatccctgtt ctctatgatc tctatctaga gtatactcgc agaaagtaca agttcaggca gatctactcc aaagaaatgt tcacacatgt ggtgacgcac agaaaggata gcatccacac actggtgtac agagagaaag agggagggca gccagatgcg tttatcacct actacatcat cgacacggca gtcctggcca aagacatcag aaagcaatat ccaaacatgc gtaacgggta catttacctt tattctctgc gcgatgactg caacctgtct ataggccagc tagtcctcgc ccttatgcac gatatgcaga acaacaatat cgatgtctgc actgctctta acgtagggct taaccccagc ttcttctcca ccatgaagtt cgcagaaggg agcggaaagc tgaattacta tctgtttaat ttgaaatggc cgaagatggc cttgaaggac ataggggtcg tgttgatata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for GilaA.18219.a.A6.GE44108
HARVESTED ON: 3/2/2021
SEQUENCED ON: 3/10/2021
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDTSIPLPE KFVWTAIDPH DDGHMADLYK LLHAYYVEDT SNMFRFAYSK DLLRWWLTSP GCKPEYSLGI RIDDPDSEGH GRLIGYISGV VSDYAYSNVD GGEVFSRPMQ SIVFLCLDKK YRSLKLAPLL IQEITRRSYK NGVFHAIYTS GTSLTAPIQV ATYYHRILNP LKLARIGFCT KPKAISDKEF AFFYKLPFLP TALSSCFHVC TEADIPVLYD LYLEYTRRKY KFRQIYSKEM FTHVVTHRKD SIHTLVYREK EGGQPDAFIT YYIIDTAVLA KDIRKQYPNM RNGYIYLYSL RDDCNLSIGQ LVLALMHDMQ NNNIDVCTAL NVGLNPSFFS TMKFAEGSGK LNYYLFNLKX XKMALKDIGV VXXX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca cttccatccc actcccggaa aagttcgtct ggacggctat tgatccccac gatgacggcc atatggccga cctctacaag cttttacacg cgtattacgt cgaagacacc agtaacatgt ttcgcttcgc gtattctaag gatcttttaa gatggtggtt gacaagtcct ggttgcaaac cagagtactc tctaggaata cgcatagacg acccagatag cgaaggccac ggaaggctca ttggttatat ttctggggtc gtgagtgact acgcctactc taacgtggac ggaggcgagg tcttttctag gcccatgcag agtattgtat ttctgtgcct cgataagaag tatcgtagtc taaagttggc cccgctgtta attcaagaga tcacacgccg ctcgtacaag aacggagtct tccatgccat atacacgtct ggaacgtctc tgaccgctcc cattcaggtt gcaacctact accaccgcat tctcaatccc ctgaagctgg caagaattgg gttttgcacg aagcctaaag caatcagcga caaggagttt gcctttttct acaagctgcc ctttcttccc actgctttgt catcgtgctt tcatgtttgc actgaagctg atatccctgt tctctatgat ctctatctag agtatactcg cagaaagtac aagttcaggc agatctactc caaagaaatg ttcacacatg tggtgacgca cagaaaggat agcatccaca cactggtgta cagagagaaa gagggagggc agccagatgc gtttatcacc tactacatca tcgacacggc agtcctggcc aaagacatca gaaagcaata tccaaacatg cgtaacgggt acatttacct ttattctctg cgcgatgact gcaacctgtc tataggccag ctagtcctcg cccttatgca cgatatgcag aacaacaata tcgatgtctg cactgctctt aacgtagggc ttaaccccag cttcttctcc accatgaagt tcgcagaagg gagcggaaag ctgaattact atctgtttaa tttgaaatgn cngaagatgg ccttgaagga cataggggtc gtgttnnnnn nnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SDTSIPLPEK FVWTAIDPHD DGHMADLYKL LHAYYVEDTS NMFRFAYSKD LLRWWLTSPG CKPEYSLGIR IDDPDSEGHG RLIGYISGVV SDYAYSNVDG GEVFSRPMQS IVFLCLDKKY RSLKLAPLLI QEITRRSYKN GVFHAIYTSG TSLTAPIQVA TYYHRILNPL KLARIGFCTK PKAISDKEFA FFYKLPFLPT ALSSCFHVCT EADIPVLYDL YLEYTRRKYK FRQIYSKEMF THVVTHRKDS IHTLVYREKE GGQPDAFITY YIIDTAVLAK DIRKQYPNMR NGYIYLYSLR DDCNLSIGQL VLALMHDMQN NNIDVCTALN VGLNPSFFST MKFAEGSGKL NYYLFNLKWP KMALKDIGVV LI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgga cacttccatc ccactcccgg aaaagttcgt ctggacggct attgatcccc acgatgacgg ccatatggcc gacctctaca agcttttaca cgcgtattac gtcgaagaca ccagtaacat gtttcgcttc gcgtattcta aggatctttt aagatggtgg ttgacaagtc ctggttgcaa accagagtac tctctaggaa tacgcataga cgacccagat agcgaaggcc acggaaggct cattggttat atttctgggg tcgtgagtga ctacgcctac tctaacgtgg acggaggcga ggtcttttct aggcccatgc agagtattgt atttctgtgc ctcgataaga agtatcgtag tctaaagttg gccccgctgt taattcaaga gatcacacgc cgctcgtaca agaacggagt cttccatgcc atatacacgt ctggaacgtc tctgaccgct cccattcagg ttgcaaccta ctaccaccgc attctcaatc ccctgaagct ggcaagaatt gggttttgca cgaagcctaa agcaatcagc gacaaggagt ttgccttttt ctacaagctg ccctttcttc ccactgcttt gtcatcgtgc tttcatgttt gcactgaagc tgatatccct gttctctatg atctctatct agagtatact cgcagaaagt acaagttcag gcagatctac tccaaagaaa tgttcacaca tgtggtgacg cacagaaagg atagcatcca cacactggtg tacagagaga aagagggagg gcagccagat gcgtttatca cctactacat catcgacacg gcagtcctgg ccaaagacat cagaaagcaa tatccaaaca tgcgtaacgg gtacatttac ctttattctc tgcgcgatga ctgcaacctg tctataggcc agctagtcct cgcccttatg cacgatatgc agaacaacaa tatcgatgtc tgcactgctc ttaacgtagg gcttaacccc agcttcttct ccaccatgaa gttcgcagaa gggagcggaa agctgaatta ctatctgttt aatttgaaat ggccgaagat ggccttgaag gacatagggg tcgtgttgat ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for GilaA.18219.a.AE4.GE44105
HARVESTED ON: 3/2/2021
SEQUENCED ON: 3/10/2021
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 85
PERCENT COVERAGE: 87
Validated AA Sequence
MDPAQHPDTS IPLPEKFVWT AIDPHDDGHM ADLYKLLHAY YVEDTSNMFR FAYSKDLLRW WLTSPGCKPE YSLGIRIDDP DSEGHGRLIG YISGVVSDYA YSNVDGGEVF SRPMQSIVFL CLDKKYRSLK LAPLLIQEIT RRSYKNGVFH AIYTSGTSLT APIQVATYYH RILNPLKLAR IGFCTKPKAI SDKEFAFFYK LPFLPTALSS CFHVCTEADI PVLYDLYLEY TRRKYKFRQI YSKEMXTHVV THRKDSIHTL VYXEKEGGQP DAFITYYIID XGSPGXRHQK AXXQTCVTGT FYLYSXAXMT AXXLXGXXVL XPYAXXXAXT TIXXXXX
Validated NT Sequence
cnnnnnnata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga aggagtctct cccatggatc ctgcccagca tcccgacact tccatcccac tcccggaaaa gttcgtctgg acggctattg atccccacga tgacggccat atggccgacc tctacaagct tttacacgcg tattacgtcg aagacaccag taacatgttt cgcttcgcgt attctaagga tcttttaaga tggtggttga caagtcctgg ttgcaaacca gagtactctc taggaatacg catagacgac ccagatagcg aaggccacgg aaggctcatt ggttatattt ctggggtcgt gagtgactac gcctactcta acgtggacgg aggcgaggtc ttttctaggc ccatgcagag tattgtattt ctgtgcctcg ataagaagta tcgtagtcta aagttggccc cgctgttaat tcaagagatc acacgccgct cgtacaagaa cggagtcttc catgccatat acacgtctgg aacgtctctg accgctccca ttcaggttgc aacctactac caccgcattc tcaatcccct gaagctggca agaattgggt tttgcacgaa gcctaaagca atcagcgaca aggagtttgc ctttttctac aagctgccct ttcttcccac tgctttgtca tcgtgctttc atgtttgcac tgaagctgat atccctgttc tctatgatct ctatctagag tatactcgca gaaagtacaa gttcaggcag atctactcca aagaaatgnt cacacatgtg gtgacgcaca gaaaggatag catccacaca ctggtgtacn nagagaaaga gggagggcag ccagatgcgt ttatcaccta ctacatcatc gacancggca gtcctggnnn aagacatcag aaagcaatnn tccaaacntg cgtaacgggt acattttacc tttattcnnc tgcgcnnatg actgcancct gnctatnagg ccanntagtc ctcnnccctt atgcnncnaa nnntgcanaa acnacnatcg annnntnnnn nncnng
Expected Protein Sequence
DPAQHPDTSI PLPEKFVWTA IDPHDDGHMA DLYKLLHAYY VEDTSNMFRF AYSKDLLRWW LTSPGCKPEY SLGIRIDDPD SEGHGRLIGY ISGVVSDYAY SNVDGGEVFS RPMQSIVFLC LDKKYRSLKL APLLIQEITR RSYKNGVFHA IYTSGTSLTA PIQVATYYHR ILNPLKLARI GFCTKPKAIS DKEFAFFYKL PFLPTALSSC FHVCTEADIP VLYDLYLEYT RRKYKFRQIY SKEMFTHVVT HRKDSIHTLV YREKEGGQPD AFITYYIIDT AVLAKDIRKQ YPNMRNGYIY LYSLRDDCNL SIGQLVLALM HDMQNNNIDV CTALNVGLNP SFFSTMKFAE GSGKLNYYLF NLKWPKMALK DIGVVLIGHH HHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccga tcctgcccag catcccgaca cttccatccc actcccggaa aagttcgtct ggacggctat tgatccccac gatgacggcc atatggccga cctctacaag cttttacacg cgtattacgt cgaagacacc agtaacatgt ttcgcttcgc gtattctaag gatcttttaa gatggtggtt gacaagtcct ggttgcaaac cagagtactc tctaggaata cgcatagacg acccagatag cgaaggccac ggaaggctca ttggttatat ttctggggtc gtgagtgact acgcctactc taacgtggac ggaggcgagg tcttttctag gcccatgcag agtattgtat ttctgtgcct cgataagaag tatcgtagtc taaagttggc cccgctgtta attcaagaga tcacacgccg ctcgtacaag aacggagtct tccatgccat atacacgtct ggaacgtctc tgaccgctcc cattcaggtt gcaacctact accaccgcat tctcaatccc ctgaagctgg caagaattgg gttttgcacg aagcctaaag caatcagcga caaggagttt gcctttttct acaagctgcc ctttcttccc actgctttgt catcgtgctt tcatgtttgc actgaagctg atatccctgt tctctatgat ctctatctag agtatactcg cagaaagtac aagttcaggc agatctactc caaagaaatg ttcacacatg tggtgacgca cagaaaggat agcatccaca cactggtgta cagagagaaa gagggagggc agccagatgc gtttatcacc tactacatca tcgacacggc agtcctggcc aaagacatca gaaagcaata tccaaacatg cgtaacgggt acatttacct ttattctctg cgcgatgact gcaacctgtc tataggccag ctagtcctcg cccttatgca cgatatgcag aacaacaata tcgatgtctg cactgctctt aacgtagggc ttaaccccag cttcttctcc accatgaagt tcgcagaagg gagcggaaag ctgaattact atctgtttaa tttgaaatgg ccgaagatgg ccttgaagga cataggggtc gtgttgatag ggcaccacca tcatcatcat taacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat