GilaA.17029.a

Metallo-beta-lactamase superfamily protein

CENTER ID:	GilaA.17029.a
ORGANISM:	Giardia lamblia ATCC 50803
ASSOCIATED DISEASE:	Giardiasis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.17029.a.A1.GE27709	Full length( GilaA.17029.a )		1	313

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	GiardiaDB:GL50803_14392
OrthoMCL:	OG5_139229
RefSeq:	XP_001708073.1
UniProt:	A8BBV2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPEDVCTVIP NHVYVVDTKF LRDKMCGVTI IRDGEEAILV EAGSTISVPH TLNALKELGI DCNNVKYMFI THAHLDHSGG TGLILQSLPN CTVVAHPAAA PHIIDPHDKL VPAAIQVYGE QVFNQDYPGI VGMPADRMIS AEDGSVYGAA SSLLKLRVIH AHGHAFHHAV FYSEALKILF AGDGLGFSFS EINFCPLLCT TPTQFDATAW RKTVDIVSKL DVDYLIATHF DCAEKNSLQR RWDCILRQLD DYEALIAASK GRDEVKSKYM GIIQKYLDEY GVKLSSERVY ALIYGDINVD GLWYRVQRNL AKA

NT Sequence

ATGCCGGAGG ACGTATGCAC AGTTATCCCC AACCACGTGT ATGTTGTGGA CACCAAATTC CTTCGCGATA AGATGTGCGG AGTTACTATC ATTCGGGACG GTGAAGAAGC GATTCTGGTC GAGGCGGGGT CGACTATTTC AGTTCCTCAC ACACTCAATG CCCTAAAAGA GCTGGGGATA GACTGCAACA ACGTCAAGTA TATGTTCATT ACTCACGCAC ATCTGGATCA CAGTGGTGGG ACTGGGCTTA TATTGCAGTC CCTGCCCAAC TGTACCGTGG TGGCACATCC AGCGGCTGCC CCCCACATAA TTGATCCTCA TGACAAGTTA GTGCCAGCAG CGATACAGGT ATATGGCGAA CAGGTCTTCA ATCAAGACTA TCCAGGGATC GTTGGAATGC CCGCCGACCG CATGATCAGC GCCGAAGATG GCTCCGTCTA CGGGGCTGCG TCTTCACTAC TCAAGCTTCG CGTCATCCAC GCTCATGGAC ACGCCTTTCA CCACGCTGTC TTCTACTCAG AGGCACTGAA GATCCTCTTT GCGGGTGATG GCCTCGGCTT TTCGTTCAGC GAAATCAACT TCTGCCCACT GCTTTGCACA ACGCCAACGC AGTTTGATGC TACAGCATGG CGCAAGACTG TCGACATCGT CAGCAAGCTT GACGTTGACT ATCTGATTGC TACTCATTTT GATTGTGCCG AGAAGAATTC ACTTCAGCGT CGCTGGGACT GCATACTGCG CCAGCTGGAT GACTATGAAG CCCTTATCGC GGCAAGTAAA GGTCGTGATG AGGTCAAATC CAAGTACATG GGTATCATTC AGAAATATTT AGACGAGTAC GGTGTCAAGC TGTCTTCCGA GCGCGTGTAC GCCCTTATCT ATGGAGACAT CAATGTTGAT GGGTTATGGT ATCGCGTTCA ACGCAATCTA GCCAAAGCG

Details for GilaA.17029.a.A1.GE27709

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMPEDVCT VIPNHVYVVD TKFLRDKMCG VTIIRDGEEA ILVEAGSTIS VPHTLNALKE LGIDCNNVKY MFITHAHLDH SGGTGLILQS LPNCTVVAHP AAAPHIIDPH DKLVPAAIQV YGEQVFNQDY PGIVGMPADR MISAEDGSVY GAASSLLKLR VIHAHGHAFH HAVFYSEALK ILFAGDGLGF SFSEINFCPL LCTTPTQFDA TAWRKTVDIV SKLDVDYLIA THFDCAEKNS LQRRWDCILR QLDDYEALIA ASKGRDEVKS KYMGIIQKYL DEYGVKLSSE RVYALIYGDI NVDGLWYRVQ RNLA

Validated NT Sequence

tggctagatt gcgttgaacg cgataccata acccatcaac attgatgtct ccatagataa gggcgtacac gcgctcggaa gacagcttga caccgtactc gtctaaatat ttctgaatga tacccatgta cttggatttg acctcatcac gacctttact tgccgcgata agggcttcat agtcatccag ctggcgcagt atgcagtccc agcgacgctg aagtgaattc ttctcggcac aatcaaaatg agtagcaatc agatagtcaa cgtcaagctt gctgacgatg tcgacagtct tgcgccatgc tgtagcatca aactgcgttg gcgttgtgca aagcagtggg cagaagttga tttcgctgaa cgaaaagccg aggccatcac ccgcaaagag gatcttcagt gcctctgagt agaagacagc gtggtgaaag gcgtgtccat gagcgtggat gacgcgaagc ttgagtagtg aagacgcagc cccgtagacg gagccatctt cggcgctgat catgcggtcg gcgggcattc caacgatccc tggatagtct tgattgaaga cctgttcgcc atatacctgt atcgctgctg gcactaactt gtcatgagga tcaattatgt ggggggcagc cgctggatgt gccaccacgg tacagttggg cagggactgc aatataagcc cagtcccacc actgtgatcc agatgtgcgt gagtaatgaa catatacttg acgttgttgc agtctatccc cagctctttt agggcattga gtgtgtgagg aactgaaata gtcgaccccg cctcgaccag aatcgcttct tcaccgtccc gaatgatagt aactccgcac atcttatcgc gaaggaattt ggtgtccaca acatacacgt ggttggggat aactgtgcat acgtcctccg gcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPEDVCTVI PNHVYVVDTK FLRDKMCGVT IIRDGEEAIL VEAGSTISVP HTLNALKELG IDCNNVKYMF ITHAHLDHSG GTGLILQSLP NCTVVAHPAA APHIIDPHDK LVPAAIQVYG EQVFNQDYPG IVGMPADRMI SAEDGSVYGA ASSLLKLRVI HAHGHAFHHA VFYSEALKIL FAGDGLGFSF SEINFCPLLC TTPTQFDATA WRKTVDIVSK LDVDYLIATH FDCAEKNSLQ RRWDCILRQL DDYEALIAAS KGRDEVKSKY MGIIQKYLDE YGVKLSSERV YALIYGDINV DGLWYRVQRN LAKA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccggaggac gtatgcacag ttatccccaa ccacgtgtat gttgtggaca ccaaattcct tcgcgataag atgtgcggag ttactatcat tcgggacggt gaagaagcga ttctggtcga ggcggggtcg actatttcag ttcctcacac actcaatgcc ctaaaagagc tggggataga ctgcaacaac gtcaagtata tgttcattac tcacgcacat ctggatcaca gtggtgggac tgggcttata ttgcagtccc tgcccaactg taccgtggtg gcacatccag cggctgcccc ccacataatt gatcctcatg acaagttagt gccagcagcg atacaggtat atggcgaaca ggtcttcaat caagactatc cagggatcgt tggaatgccc gccgaccgca tgatcagcgc cgaagatggc tccgtctacg gggctgcgtc ttcactactc aagcttcgcg tcatccacgc tcatggacac gcctttcacc acgctgtctt ctactcagag gcactgaaga tcctctttgc gggtgatggc ctcggctttt cgttcagcga aatcaacttc tgcccactgc tttgcacaac gccaacgcag tttgatgcta cagcatggcg caagactgtc gacatcgtca gcaagcttga cgttgactat ctgattgcta ctcattttga ttgtgccgag aagaattcac ttcagcgtcg ctgggactgc atactgcgcc agctggatga ctatgaagcc cttatcgcgg caagtaaagg tcgtgatgag gtcaaatcca agtacatggg tatcattcag aaatatttag acgagtacgg tgtcaagctg tcttccgagc gcgtgtacgc ccttatctat ggagacatca atgttgatgg gttatggtat cgcgttcaac gcaatctagc caaagcgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa