GilaA.10478.a
Branched-chain amino acid aminotransferase lateral transfer candidate
| CENTER ID: | GilaA.10478.a |
| ORGANISM: | Giardia lamblia ATCC 50803 |
| ASSOCIATED DISEASE: | Giardiasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.10478.a.A1.GE27706 | Full length( GilaA.10478.a ) | 1 | 355 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.10478.a.A1.PW27569 | Full length( GilaA.10478.a ) | 1 | 355 |
Structures
7LV7
DEPOSITED: 2/24/2021
DETERMINATION: XRay
CLONE: GilaA.10478.a.A1.GE27706
PROTEIN: GilaA.10478.a.A1.PW27569
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | GiardiaDB:GL50803_6184 |
| RefSeq: | XP_001706262.1 |
| UniProt: | Q86QZ8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAVDPSTIDW SALKFSWLQT RSHVRSVWRN GEWSPLELVN EPTFNISIAA SALHYGQAVF EGLKVFRTVD GRVAAFRPVE NARRLISSCD GLCMESPSEQ LFLNALAMVV RDNVDYIPPY GTGGSLYVRP LVIGTGAQLG VAPSSEYMFL MMVAPVGPYY RGGLKSVNAI VMDEFDRAAP YGVGSKKCAG NYAASLKAQS VALKKSFPIQ LYLDAATHTF VEEFSTSNFF GIKDIQRDGA GKIVSCTYVT PKSPSILPSI TNKTLRELIS QYFGWKVDVR EVPFTEVKTF QECGATGTAV VVTPIASITR GSTVIDFLQS DDQVGEVTKL LYETVQGIQY GVIPDRFNWN HYIDV
NT Sequence
ATGGCTGTCG ATCCCTCCAC GATAGACTGG TCTGCGCTGA AGTTTTCGTG GCTGCAAACG CGCTCCCACG TTCGCAGTGT CTGGAGAAAC GGCGAGTGGA GCCCTCTTGA GCTGGTAAAC GAGCCGACAT TCAATATTTC GATTGCGGCC TCTGCGCTTC ACTACGGCCA GGCAGTCTTC GAGGGTCTCA AGGTCTTCCG CACTGTTGAC GGCAGAGTCG CTGCCTTCAG ACCAGTTGAG AATGCCCGCA GGCTCATCAG CTCGTGCGAC GGTCTCTGCA TGGAGTCCCC GTCAGAGCAG CTCTTCCTCA ATGCTCTTGC GATGGTTGTC AGGGATAACG TTGACTATAT CCCGCCATAT GGTACAGGGG GCTCCCTCTA TGTCCGTCCC CTGGTTATCG GTACCGGAGC CCAGCTTGGA GTTGCACCGT CGTCCGAATA CATGTTCCTC ATGATGGTCG CGCCTGTTGG TCCTTATTAC CGGGGAGGCC TCAAGAGCGT GAACGCAATC GTTATGGACG AATTCGACAG GGCAGCACCC TATGGCGTCG GCAGCAAGAA GTGTGCCGGA AACTACGCCG CATCTCTTAA GGCCCAGAGC GTTGCTCTGA AAAAGAGCTT CCCCATTCAG CTGTACCTCG ACGCTGCTAC GCACACGTTT GTTGAGGAGT TCTCCACCAG CAACTTCTTT GGGATTAAGG ACATCCAGAG AGATGGGGCG GGCAAGATCG TTTCGTGCAC TTACGTTACT CCTAAGTCTC CTTCTATTTT GCCCTCGATC ACGAACAAGA CTCTCCGCGA GCTTATTTCC CAGTACTTCG GCTGGAAGGT TGATGTGCGC GAGGTCCCGT TCACAGAGGT TAAGACATTC CAAGAATGCG GAGCCACAGG CACGGCCGTC GTTGTGACGC CCATTGCTTC CATCACCCGC GGTTCCACTG TTATCGACTT CCTGCAATCT GACGATCAGG TTGGCGAAGT TACCAAGCTA CTATATGAAA CTGTCCAGGG CATACAGTAC GGGGTGATCC CAGACAGATT CAACTGGAAC CACTACATTG ACGTG
Details for GilaA.10478.a.A1.GE27706
| HARVESTED ON: | 10/13/2009 |
| SEQUENCED ON: | 10/21/2009 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAVDPSTID WSALKFSWLQ TRSHVRSVWR NGEWSPLELV NEPTFNISIA ASALHYGQAV FEGLKVFRTV DGRVAAFRPV ENARRLISSC DGLCMESPSE QLFLNALAMV VRDNVDYIPP YGTGGSLYVR PLVIGTGAQL GVAPSSEYMF LMMVAPVGPY YRGGLKSVNA IVMDEFDRAA PYGVGSKKCA GNYAASLKAQ SVALKKSFPI QLYLDAATHT FVEEFSTSNF FGIKDIQRDG AGKIVSCTYV TPKSPSILPS ITNKTLRELI SQYFGWKVDV REVPFTEVKT FQECGATGTA VVVTPIASIT RGSTVIDFLQ SDDQVGEVTK LLYETVQGIQ YGVIPDRFNW NHYI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg tcgatccctc cacgatagac tggtctgcgc tgaagttttc gtggctgcaa acgcgctccc acgttcgcag tgtctggaga aacggcgagt ggagccctct tgagctggta aacgagccga cattcaatat ttcgattgcg gcctctgcgc ttcactacgg ccaggcagtc ttcgagggtc tcaaggtctt ccgcactgtt gacggcagag tcgctgcctt cagaccagtt gagaatgccc gcaggctcat cagctcgtgc gacggtctct gcatggagtc cccgtcagag cagctcttcc tcaatgctct tgcgatggtt gtcagggata acgttgacta tatcccgcca tatggtacag ggggctccct ctatgtccgt cccctggtta tcggtaccgg agcccagctt ggagttgcac cgtcgtccga atacatgttc ctcatgatgg tcgcgcctgt tggtccttat taccggggag gcctcaagag cgtgaacgca atcgttatgg acgaattcga cagggcagca ccctatggcg tcggcagcaa gaagtgtgcc ggaaactacg ccgcgtctct taaggcccag agcgttgctc tgaaaaagag cttccccatt cagctgtacc tcgacgctgc tacgcacacg tttgttgagg agttctccac cagcaacttc tttgggatta aggacatcca gagagatggg gcgggcaaga tcgtttcgtg cacttacgtt actcctaagt ctccttctat tttgccctcg atcacgaaca agactctccg cgagcttatt tcccagtact tcggctggaa ggttgatgtg cgcgaggtcc cgttcacaga ggttaagaca ttccaagaat gcggagccac aggcacggcc gtcgttgtga cgcccattgc ttccatcacc cgcggttcca ctgttatcga cttcctgcaa tctgacgatc aggttggcga agttaccaag ctactatatg aaactgtcca gggcatacag tacggggtga tcccagacag attcaactgg aaccactaca ttg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVDPSTID WSALKFSWLQ TRSHVRSVWR NGEWSPLELV NEPTFNISIA ASALHYGQAV FEGLKVFRTV DGRVAAFRPV ENARRLISSC DGLCMESPSE QLFLNALAMV VRDNVDYIPP YGTGGSLYVR PLVIGTGAQL GVAPSSEYMF LMMVAPVGPY YRGGLKSVNA IVMDEFDRAA PYGVGSKKCA GNYAASLKAQ SVALKKSFPI QLYLDAATHT FVEEFSTSNF FGIKDIQRDG AGKIVSCTYV TPKSPSILPS ITNKTLRELI SQYFGWKVDV REVPFTEVKT FQECGATGTA VVVTPIASIT RGSTVIDFLQ SDDQVGEVTK LLYETVQGIQ YGVIPDRFNW NHYIDV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgtcgat ccctccacga tagactggtc tgcgctgaag ttttcgtggc tgcaaacgcg ctcccacgtt cgcagtgtct ggagaaacgg cgagtggagc cctcttgagc tggtaaacga gccgacattc aatatttcga ttgcggcctc tgcgcttcac tacggccagg cagtcttcga gggtctcaag gtcttccgca ctgttgacgg cagagtcgct gccttcagac cagttgagaa tgcccgcagg ctcatcagct cgtgcgacgg tctctgcatg gagtccccgt cagagcagct cttcctcaat gctcttgcga tggttgtcag ggataacgtt gactatatcc cgccatatgg tacagggggc tccctctatg tccgtcccct ggttatcggt accggagccc agcttggagt tgcaccgtcg tccgaataca tgttcctcat gatggtcgcg cctgttggtc cttattaccg gggaggcctc aagagcgtga acgcaatcgt tatggacgaa ttcgacaggg cagcacccta tggcgtcggc agcaagaagt gtgccggaaa ctacgccgca tctcttaagg cccagagcgt tgctctgaaa aagagcttcc ccattcagct gtacctcgac gctgctacgc acacgtttgt tgaggagttc tccaccagca acttctttgg gattaaggac atccagagag atggggcggg caagatcgtt tcgtgcactt acgttactcc taagtctcct tctattttgc cctcgatcac gaacaagact ctccgcgagc ttatttccca gtacttcggc tggaaggttg atgtgcgcga ggtcccgttc acagaggtta agacattcca agaatgcgga gccacaggca cggccgtcgt tgtgacgccc attgcttcca tcacccgcgg ttccactgtt atcgacttcc tgcaatctga cgatcaggtt ggcgaagtta ccaagctact atatgaaact gtccagggca tacagtacgg ggtgatccca gacagattca actggaacca ctacattgac gtgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for GilaA.10478.a.A1.PW27569
| PURIFICATION DATe: | 1/11/2009 |
| CONCENTRATION: | 25.54mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 23 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAVDPSTID WSALKFSWLQ TRSHVRSVWR NGEWSPLELV NEPTFNISIA ASALHYGQAV FEGLKVFRTV DGRVAAFRPV ENARRLISSC DGLCMESPSE QLFLNALAMV VRDNVDYIPP YGTGGSLYVR PLVIGTGAQL GVAPSSEYMF LMMVAPVGPY YRGGLKSVNA IVMDEFDRAA PYGVGSKKCA GNYAASLKAQ SVALKKSFPI QLYLDAATHT FVEEFSTSNF FGIKDIQRDG AGKIVSCTYV TPKSPSILPS ITNKTLRELI SQYFGWKVDV REVPFTEVKT FQECGATGTA VVVTPIASIT RGSTVIDFLQ SDDQVGEVTK LLYETVQGIQ YGVIPDRFNW NHYI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg tcgatccctc cacgatagac tggtctgcgc tgaagttttc gtggctgcaa acgcgctccc acgttcgcag tgtctggaga aacggcgagt ggagccctct tgagctggta aacgagccga cattcaatat ttcgattgcg gcctctgcgc ttcactacgg ccaggcagtc ttcgagggtc tcaaggtctt ccgcactgtt gacggcagag tcgctgcctt cagaccagtt gagaatgccc gcaggctcat cagctcgtgc gacggtctct gcatggagtc cccgtcagag cagctcttcc tcaatgctct tgcgatggtt gtcagggata acgttgacta tatcccgcca tatggtacag ggggctccct ctatgtccgt cccctggtta tcggtaccgg agcccagctt ggagttgcac cgtcgtccga atacatgttc ctcatgatgg tcgcgcctgt tggtccttat taccggggag gcctcaagag cgtgaacgca atcgttatgg acgaattcga cagggcagca ccctatggcg tcggcagcaa gaagtgtgcc ggaaactacg ccgcgtctct taaggcccag agcgttgctc tgaaaaagag cttccccatt cagctgtacc tcgacgctgc tacgcacacg tttgttgagg agttctccac cagcaacttc tttgggatta aggacatcca gagagatggg gcgggcaaga tcgtttcgtg cacttacgtt actcctaagt ctccttctat tttgccctcg atcacgaaca agactctccg cgagcttatt tcccagtact tcggctggaa ggttgatgtg cgcgaggtcc cgttcacaga ggttaagaca ttccaagaat gcggagccac aggcacggcc gtcgttgtga cgcccattgc ttccatcacc cgcggttcca ctgttatcga cttcctgcaa tctgacgatc aggttggcga agttaccaag ctactatatg aaactgtcca gggcatacag tacggggtga tcccagacag attcaactgg aaccactaca ttg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVDPSTID WSALKFSWLQ TRSHVRSVWR NGEWSPLELV NEPTFNISIA ASALHYGQAV FEGLKVFRTV DGRVAAFRPV ENARRLISSC DGLCMESPSE QLFLNALAMV VRDNVDYIPP YGTGGSLYVR PLVIGTGAQL GVAPSSEYMF LMMVAPVGPY YRGGLKSVNA IVMDEFDRAA PYGVGSKKCA GNYAASLKAQ SVALKKSFPI QLYLDAATHT FVEEFSTSNF FGIKDIQRDG AGKIVSCTYV TPKSPSILPS ITNKTLRELI SQYFGWKVDV REVPFTEVKT FQECGATGTA VVVTPIASIT RGSTVIDFLQ SDDQVGEVTK LLYETVQGIQ YGVIPDRFNW NHYIDV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGTCGAT CCCTCCACGA TAGACTGGTC TGCGCTGAAG TTTTCGTGGC TGCAAACGCG CTCCCACGTT CGCAGTGTCT GGAGAAACGG CGAGTGGAGC CCTCTTGAGC TGGTAAACGA GCCGACATTC AATATTTCGA TTGCGGCCTC TGCGCTTCAC TACGGCCAGG CAGTCTTCGA GGGTCTCAAG GTCTTCCGCA CTGTTGACGG CAGAGTCGCT GCCTTCAGAC CAGTTGAGAA TGCCCGCAGG CTCATCAGCT CGTGCGACGG TCTCTGCATG GAGTCCCCGT CAGAGCAGCT CTTCCTCAAT GCTCTTGCGA TGGTTGTCAG GGATAACGTT GACTATATCC CGCCATATGG TACAGGGGGC TCCCTCTATG TCCGTCCCCT GGTTATCGGT ACCGGAGCCC AGCTTGGAGT TGCACCGTCG TCCGAATACA TGTTCCTCAT GATGGTCGCG CCTGTTGGTC CTTATTACCG GGGAGGCCTC AAGAGCGTGA ACGCAATCGT TATGGACGAA TTCGACAGGG CAGCACCCTA TGGCGTCGGC AGCAAGAAGT GTGCCGGAAA CTACGCCGCA TCTCTTAAGG CCCAGAGCGT TGCTCTGAAA AAGAGCTTCC CCATTCAGCT GTACCTCGAC GCTGCTACGC ACACGTTTGT TGAGGAGTTC TCCACCAGCA ACTTCTTTGG GATTAAGGAC ATCCAGAGAG ATGGGGCGGG CAAGATCGTT TCGTGCACTT ACGTTACTCC TAAGTCTCCT TCTATTTTGC CCTCGATCAC GAACAAGACT CTCCGCGAGC TTATTTCCCA GTACTTCGGC TGGAAGGTTG ATGTGCGCGA GGTCCCGTTC ACAGAGGTTA AGACATTCCA AGAATGCGGA GCCACAGGCA CGGCCGTCGT TGTGACGCCC ATTGCTTCCA TCACCCGCGG TTCCACTGTT ATCGACTTCC TGCAATCTGA CGATCAGGTT GGCGAAGTTA CCAAGCTACT ATATGAAACT GTCCAGGGCA TACAGTACGG GGTGATCCCA GACAGATTCA ACTGGAACCA CTACATTGAC GTGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA