GilaA.10201.a

Methionyl-tRNA synthetase (MetRS)

CENTER ID: GilaA.10201.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
GilaA.10201.a.B1.GE43489 full length 1 582
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_22204
UniProt: A8BY75

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEARKVYLTT PIYYVNGKPH IGHVFTTLLA DTLKRFRKLQ GFETYLMTGT DEHGQKVQQS AEKMGLQPQQ WCDNMAKTFE TYFLENFGLC PDVFMRTTMP KHVEMATKLW EKLRTAGLIY KDKYEGWYCV ADEAFVTETN LKDGVDAQGK PCKVSAESGQ PCVFLSEENY KFRLSSFQEP LLKFYQDNPE IIIPAFRQAE IVSFIHSGLH DISISRSKDK ISWGIPVPGD SDHVMYVWID ALAIYLTAAD STINPDGTFS SRLWPADVQF LGKDIARFHC VYWLAFLMGA SIPCPRRFFI HGWWTINHQK IGKSLGNALD PMSLVEAYGL DGVKYFMLRE GSPANDPGIT EELISMRTES DLANVLGNLT MRCTADSLLP DRAWTDPSKL FEGVSFAQIR SLWMKSRRSS IAELLPADAG ERENISSIIK INDISDLLDK GLAICEEVTN LMDNIDIPGA LSKIFEILVG INNYIQLTMP WVLIKKAETK NGHEFVMYCA NELLRVCATL LLPFIPQKAP LILDQLGVSS EDRVIKREVL TIGYIAPGLT LGEKLPPLFP KQSSKKQGTA PAQGKAKNGK KK
NT Sequence
atggaggcaa gaaaggtata tcttaccacg cctatttact atgttaatgg aaagcctcac atcggtcatg tcttcacaac gctgcttgca gacactttga agcgctttag aaaactccaa ggttttgaga cttacctcat gactggaacc gatgagcacg gccagaaagt ccagcagtct gccgagaaaa tgggactgca accgcagcaa tggtgtgata acatggctaa gacatttgaa acgtactttc tggagaactt tggtctgtgc ccggatgtct ttatgcgcac gacgatgcct aagcatgttg agatggctac caaactttgg gagaagctgc gtactgctgg actgatctat aaagataagt atgaaggttg gtattgtgtt gccgacgaag cttttgtaac agagacaaat ctcaaggatg gtgttgatgc tcaagggaag ccatgcaaag tgtctgcaga gtctggccaa ccttgtgtgt ttttgagcga ggaaaactat aagttcagac tctcgtcatt tcaggagccc ttgctcaaat tttaccagga caatcctgag atcatcatac ccgcctttcg acaggccgaa atagtctcct tcattcactc ggggcttcac gatatctcca tttcccgttc caaagacaag atctcatggg ggatccctgt gccaggggac agtgaccacg ttatgtacgt ctggattgac gcactagcta tatacctaac cgctgctgat tctacaatca atccagatgg aacatttagc agtaggttgt ggcctgccga tgtccagttt ttggggaaag atattgccag attccactgt gtctattggc ttgcgtttct aatgggcgca agcataccgt gtcctcgacg ctttttcatc catgggtggt ggacaataaa tcaccagaaa atcgggaaaa gtctcggtaa tgccctcgat ccaatgtccc tggtggaggc atatggcttg gacggcgtta aatacttcat gttgcgtgaa ggttctccag caaatgaccc cggtattacg gaggaactta tctcgatgcg tacagaatca gacttagcga atgttctagg caacctgacc atgcgttgca ccgcagattc gttactaccg gacagagcct ggacagatcc gtcgaagcta tttgaaggcg taagctttgc acagatccgg tctctttgga tgaaaagtag aaggagctct atagcagaac tattgccagc tgacgcgggt gagcgtgaaa atatttcttc tataataaaa atcaacgata tctctgatct tctggataaa ggacttgcga tttgtgagga agtgaccaac cttatggaca atatcgacat tccaggagct ctttccaaga tatttgagat tttagttggg attaataatt atattcaact gacgatgccg tgggtcctga tcaagaaggc ggaaaccaag aatggccatg aattcgtcat gtactgcgct aatgaacttt tacgtgtctg tgcaaccctc ttgcttccgt ttataccaca gaaggccccg ctcatcttag accaacttgg agtttcgagt gaagaccgag ttattaaaag ggaagtcttg accattggct acattgcgcc aggattgacg ctgggtgaga aattgccgcc cctgtttcca aaacagagct cgaaaaagca aggcactgca ccagctcaag gcaaagctaa gaacggaaag aagaagtaa
Details for GilaA.10201.a.B1.GE43489
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME ARKVYLTTPI YYVNGKPHIG HVFTTLLADT LKRFRKLQGF ETYLMTGTDE HGQKVQQSAE KMGLQPQQWC DNMAKTFETY FLENFGLCPD VFMRTTMPKH VEMATKLWEK LRTAGLIYKD KYEGWYCVAD EAFVTETNLK DGVDAQGKPC KVSAESGQPC VFLSEENYKF RLSSFQEPLL KFYQDNPEII IPAFRQAEIV SFIHSGLHDI SISRSKDKIS WGIPVPGDSD HVMYVWIDAL XIYLTAAXST IXPXGTFXSX XWXADVQFLG KDIARFHCVY WXAXXMGASI PXPRRFFXHG WXXIXXQKIG KSLXNALDXM SLVEAYXLDG VKYFMLREXX PANDPGITEE LISMRTESDL ANVLGNLTMR CTADSLLPDR AWTDPSKLFE GVSFAQIRSL WMKSRRSSIA ELLPADAGER ENISSIIKIN DISDLLDKGL AICEEVTNLM DNIDIPGALS KIFEILVGIN NYIQLTMPWV LIKKAETKNG HEFVMYCANE LLRVCATLLL PFIPQKAPLI LDQLGVSSED RVIKREVLTI GYIAPGLTLG EKLPPLFPKQ SSKKQGTAPA QGK
Validated NT Sequence
agctttgcct tgagctggtg cagtgccttg ctttttcgag ctctgttttg gaaacagggg cggcaatttc tcacccagcg tcaatcctgg cgcaatgtag ccaatggtca agacttccct tttaataact cggtcttcac tcgaaactcc aagttggtct aagatgagcg gggccttctg tggtataaac ggaagcaaga gggttgcaca gacacgtaaa agttcattag cgcagtacat gacgaattca tggccattct tggtttccgc cttcttgatc aggacccacg gcatcgtcag ttgaatataa ttattaatcc caactaaaat ctcaaatatc ttggaaagag ctcctggaat gtcgatattg tccataaggt tggtcacttc ctcacaaatc gcaagtcctt tatccagaag atcagagata tcgttgattt ttattataga agaaatattt tcacgctcac ccgcgtcagc tggcaatagt tctgctatag agctccttct acttttcatc caaagagacc ggatctgtgc aaagcttacg ccttcaaata gcttcgacgg atctgtccag gctctgtccg gtagtaacga atctgcggtg caacgcatgg tcaggttgcc tagaacattc gctaagtctg attctgtacg catcgagata agttcctccg taataccggg gtcatttgct gganannctt cacgcaacat gaagtattta acgccgtcca agncatatgc ctccaccagg gacattgnat cgagggcatt ancgagactt ttcccgattt tctggngant tattgnccnc cacccatgga ngaaaaagcg tcgaggacnc ggtatgcttg cgcccattan naacgcaanc caatagacac agtggaatct ggcaatatct ttccccaaaa actggacatc ggcagnccac annnnactgn taaatgttcc ntntggatng attgtagant cagcagcggt taggtatata gntagtgcgt caatccagac gtacataacg tggtcactgt cccctggcac agggatcccc catgagatct tgtctttgga acgggaaatg gagatatcgt gaagccccga gtgaatgaag gagactattt cggcctgtcg aaaggcgggt atgatgatct caggattgtc ctggtaaaat ttgagcaagg gctcctgaaa tgacgagagt ctgaacttat agttttcctc gctcaaaaac acacaaggtt ggccagactc tgcagacact ttgcatggct tcccttgagc atcaacacca tccttgagat ttgtctctgt tacaaaagct tcgtcggcaa cacaatacca accttcatac ttatctttat agatcagtcc agcagtacgc agcttctccc aaagtttggt agccatctca acatgcttag gcatcgtcgt gcgcataaag acatccgggc acagaccaaa gttctccaga aagtacgttt caaatgtctt agccatgtta tcacaccatt gctgcggttg cagtcccatt ttctcggcag actgctggac tttctggccg tgctcatcgg ttccagtcat gaggtaagtc tcaaaacctt ggagttttct aaagcgcttc aaagtgtctg caagcagcgt tgtgaagaca tgaccgatgt gaggctttcc attaacatag taaataggcg tggtaagata tacctttctt gcctccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHME ARKVYLTTPI YYVNGKPHIG HVFTTLLADT LKRFRKLQGF ETYLMTGTDE HGQKVQQSAE KMGLQPQQWC DNMAKTFETY FLENFGLCPD VFMRTTMPKH VEMATKLWEK LRTAGLIYKD KYEGWYCVAD EAFVTETNLK DGVDAQGKPC KVSAESGQPC VFLSEENYKF RLSSFQEPLL KFYQDNPEII IPAFRQAEIV SFIHSGLHDI SISRSKDKIS WGIPVPGDSD HVMYVWIDAL AIYLTAADST INPDGTFSSR LWPADVQFLG KDIARFHCVY WLAFLMGASI PCPRRFFIHG WWTINHQKIG KSLGNALDPM SLVEAYGLDG VKYFMLREGS PANDPGITEE LISMRTESDL ANVLGNLTMR CTADSLLPDR AWTDPSKLFE GVSFAQIRSL WMKSRRSSIA ELLPADAGER ENISSIIKIN DISDLLDKGL AICEEVTNLM DNIDIPGALS KIFEILVGIN NYIQLTMPWV LIKKAETKNG HEFVMYCANE LLRVCATLLL PFIPQKAPLI LDQLGVSSED RVIKREVLTI GYIAPGLTLG EKLPPLFPKQ SSKKQGTAPA QGKAKNGKKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagg caagaaaggt atatcttacc acgcctattt actatgttaa tggaaagcct cacatcggtc atgtcttcac aacgctgctt gcagacactt tgaagcgctt tagaaaactc caaggttttg agacttacct catgactgga accgatgagc acggccagaa agtccagcag tctgccgaga aaatgggact gcaaccgcag caatggtgtg ataacatggc taagacattt gaaacgtact ttctggagaa ctttggtctg tgcccggatg tctttatgcg cacgacgatg cctaagcatg ttgagatggc taccaaactt tgggagaagc tgcgtactgc tggactgatc tataaagata agtatgaagg ttggtattgt gttgccgacg aagcttttgt aacagagaca aatctcaagg atggtgttga tgctcaaggg aagccatgca aagtgtctgc agagtctggc caaccttgtg tgtttttgag cgaggaaaac tataagttca gactctcgtc atttcaggag cccttgctca aattttacca ggacaatcct gagatcatca tacccgcctt tcgacaggcc gaaatagtct ccttcattca ctcggggctt cacgatatct ccatttcccg ttccaaagac aagatctcat gggggatccc tgtgccaggg gacagtgacc acgttatgta cgtctggatt gacgcactag ctatatacct aaccgctgct gattctacaa tcaatccaga tggaacattt agcagtaggt tgtggcctgc cgatgtccag tttttgggga aagatattgc cagattccac tgtgtctatt ggcttgcgtt tctaatgggc gcaagcatac cgtgtcctcg acgctttttc atccatgggt ggtggacaat aaatcaccag aaaatcggga aaagtctcgg taatgccctc gatccaatgt ccctggtgga ggcatatggc ttggacggcg ttaaatactt catgttgcgt gaaggttctc cagcaaatga ccccggtatt acggaggaac ttatctcgat gcgtacagaa tcagacttag cgaatgttct aggcaacctg accatgcgtt gcaccgcaga ttcgttacta ccggacagag cctggacaga tccgtcgaag ctatttgaag gcgtaagctt tgcacagatc cggtctcttt ggatgaaaag tagaaggagc tctatagcag aactattgcc agctgacgcg ggtgagcgtg aaaatatttc ttctataata aaaatcaacg atatctctga tcttctggat aaaggacttg cgatttgtga ggaagtgacc aaccttatgg acaatatcga cattccagga gctctttcca agatatttga gattttagtt gggattaata attatattca actgacgatg ccgtgggtcc tgatcaagaa ggcggaaacc aagaatggcc atgaattcgt catgtactgc gctaatgaac ttttacgtgt ctgtgcaacc ctcttgcttc cgtttatacc acagaaggcc ccgctcatct tagaccaact tggagtttcg agtgaagacc gagttattaa aagggaagtc ttgaccattg gctacattgc gccaggattg acgctgggtg agaaattgcc gcccctgttt ccaaaacaga gctcgaaaaa gcaaggcact gcaccagctc aaggcaaagc taagaacgga aagaagaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat