GilaA.01493.a

Hypothetical protein, enzyme with a TIM-barrel fold

CENTER ID: GilaA.01493.a
ORGANISM: Giardia lamblia ATCC 50803
ASSOCIATED DISEASE: Giardiasis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01493.a.A1.GE25751 Full length( GilaA.01493.a ) 1 230
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
GilaA.01493.a.A1.PS00123 Full length( GilaA.01493.a ) 1 230

External Resources

RESOURCE REFERENCE ID
EuPathDB: GiardiaDB:GL50803_15041
RefSeq: XP_001707349.1
UniProt: A8BFV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTVAGALRD IRAGLGHAKL IAVSKTKPVE AILEAYAAGQ RLFGENYVQE LVKKAEAVQG VASDIEWHFI GHLQTNKARD VAFIPNCVVQ TVDSDRLARR LSELRPDELG PLRVMIQINI SGELTKSGCT VEDAIELAQL ISALPRLRLI GLMTIGAPDA SEYSFRALVD ARNVIEQAVK LEEKLELSMG MSSDYQLAVK MGADYVRVGS AIFGERTYSQ QKGQLQNSDE
NT Sequence
ATGTCTACCG TTGCCGGCGC TCTCAGAGAT ATTAGGGCAG GATTAGGACA CGCAAAGCTC ATTGCTGTCT CAAAGACAAA GCCAGTAGAG GCAATTCTCG AGGCGTATGC GGCCGGCCAG CGTCTGTTTG GAGAGAACTA TGTCCAGGAA CTAGTGAAAA AGGCTGAGGC CGTGCAGGGC GTGGCAAGCG ATATTGAGTG GCACTTTATT GGCCACCTAC AAACTAATAA AGCACGAGAC GTCGCCTTCA TTCCAAATTG TGTTGTCCAG ACAGTGGATT CTGATAGGCT TGCCAGGCGA TTGAGCGAAT TGCGACCAGA TGAGCTGGGT CCGTTACGGG TGATGATACA GATTAACATT AGCGGTGAGC TCACAAAAAG CGGGTGCACC GTCGAAGATG CAATTGAATT GGCTCAGTTG ATTAGTGCCC TTCCTCGACT TAGACTTATT GGACTCATGA CCATAGGAGC CCCAGATGCC TCGGAATACT CCTTCAGAGC ACTTGTGGAC GCCCGCAATG TCATCGAGCA AGCCGTCAAG CTTGAGGAGA AGCTTGAGCT CTCCATGGGG ATGAGTTCCG ACTATCAATT GGCGGTGAAG ATGGGCGCTG ACTACGTCCG AGTTGGATCT GCCATCTTTG GCGAGCGCAC TTATTCACAA CAGAAGGGTC AACTTCAAAA TTCGGATGAG
Details for GilaA.01493.a.A1.GE25751
HARVESTED ON: 10/31/2008
SEQUENCED ON: 7/20/2010
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTVAGALR DIRAGLGHAK LIAVSKTKPV EAILEAYAAG QRLFGENYVQ ELVKKAEAVQ GVASDIEWHF IGHLQTNKAR DVAFIPNCVV QTVDSDRLAR RLSELRPDEL GPLRVMIQIN ISGELTKSGC TVEDAIELAQ LISALPRLRL IGLMTIGAPD ASEYSFRALV DARNVIEQAV KLEEKLELSM GMSSDYQLAV KMGADYVRVG SAIFGERTYS QQKGQLQNSD E
Validated NT Sequence
tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctac cgttgccggc gctctcagag atattagggc aggattagga cacgcaaagc tcattgctgt ctcaaagaca aagccagtag aggcaattct cgaggcgtat gcggccggcc agcgtctgtt tggagagaac tatgtccagg aactagtgaa aaaggctgag gccgtgcagg gcgtggcaag cgatattgag tggcacttta ttggccacct acaaactaat aaagcacgag acgtcgcctt cattccaaat tgtgttgtcc agacagtgga ttctgatagg cttgccaggc gattgagcga attgcgacca gatgagctgg gtccgttacg ggtgatgata cagattaaca ttagcggtga gctcacaaaa agcgggtgca ccgtcgaaga tgcaattgaa ttggctcagt tgattagtgc ccttccccga cttagactta ttggactcat gaccatagga gccccagatg cctcggaata ctccttcaga gcacttgtgg acgcccgcaa tgtcatcgag caagccgtca agcttgagga gaagcttgag ctctccatgg ggatgagttc cgactatcaa ttggcggtga agatgggcgc tgactacgtc cgagttggat ctgccatctt tggcgagcgc acttattcac aacagaaggg tcaacttcaa aattcggatg agtaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTVAGALR DIRAGLGHAK LIAVSKTKPV EAILEAYAAG QRLFGENYVQ ELVKKAEAVQ GVASDIEWHF IGHLQTNKAR DVAFIPNCVV QTVDSDRLAR RLSELRPDEL GPLRVMIQIN ISGELTKSGC TVEDAIELAQ LISALPRLRL IGLMTIGAPD ASEYSFRALV DARNVIEQAV KLEEKLELSM GMSSDYQLAV KMGADYVRVG SAIFGERTYS QQKGQLQNSD E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctaccgtt gccggcgctc tcagagatat tagggcagga ttaggacacg caaagctcat tgctgtctca aagacaaagc cagtagaggc aattctcgag gcgtatgcgg ccggccagcg tctgtttgga gagaactatg tccaggaact agtgaaaaag gctgaggccg tgcagggcgt ggcaagcgat attgagtggc actttattgg ccacctacaa actaataaag cacgagacgt cgccttcatt ccaaattgtg ttgtccagac agtggattct gataggcttg ccaggcgatt gagcgaattg cgaccagatg agctgggtcc gttacgggtg atgatacaga ttaacattag cggtgagctc acaaaaagcg ggtgcaccgt cgaagatgca attgaattgg ctcagttgat tagtgccctt cctcgactta gacttattgg actcatgacc ataggagccc cagatgcctc ggaatactcc ttcagagcac ttgtggacgc ccgcaatgtc atcgagcaag ccgtcaagct tgaggagaag cttgagctct ccatggggat gagttccgac tatcaattgg cggtgaagat gggcgctgac tacgtccgag ttggatctgc catctttggc gagcgcactt attcacaaca gaagggtcaa cttcaaaatt cggatgagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for GilaA.01493.a.A1.PS00123
PURIFICATION DATe: 1/17/2009
CONCENTRATION: 12.96mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTVAGALR DIRAGLGHAK LIAVSKTKPV EAILEAYAAG QRLFGENYVQ ELVKKAEAVQ GVASDIEWHF IGHLQTNKAR DVAFIPNCVV QTVDSDRLAR RLSELRPDEL GPLRVMIQIN ISGELTKSGC TVEDAIELAQ LISALPRLRL IGLMTIGAPD ASEYSFRALV DARNVIEQAV KLEEKLELSM GMSSDYQLAV KMGADYVRVG SAIFGERTYS QQKGQLQNSD E
Validated NT Sequence
tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctac cgttgccggc gctctcagag atattagggc aggattagga cacgcaaagc tcattgctgt ctcaaagaca aagccagtag aggcaattct cgaggcgtat gcggccggcc agcgtctgtt tggagagaac tatgtccagg aactagtgaa aaaggctgag gccgtgcagg gcgtggcaag cgatattgag tggcacttta ttggccacct acaaactaat aaagcacgag acgtcgcctt cattccaaat tgtgttgtcc agacagtgga ttctgatagg cttgccaggc gattgagcga attgcgacca gatgagctgg gtccgttacg ggtgatgata cagattaaca ttagcggtga gctcacaaaa agcgggtgca ccgtcgaaga tgcaattgaa ttggctcagt tgattagtgc ccttccccga cttagactta ttggactcat gaccatagga gccccagatg cctcggaata ctccttcaga gcacttgtgg acgcccgcaa tgtcatcgag caagccgtca agcttgagga gaagcttgag ctctccatgg ggatgagttc cgactatcaa ttggcggtga agatgggcgc tgactacgtc cgagttggat ctgccatctt tggcgagcgc acttattcac aacagaaggg tcaacttcaa aattcggatg agtaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTVAGALR DIRAGLGHAK LIAVSKTKPV EAILEAYAAG QRLFGENYVQ ELVKKAEAVQ GVASDIEWHF IGHLQTNKAR DVAFIPNCVV QTVDSDRLAR RLSELRPDEL GPLRVMIQIN ISGELTKSGC TVEDAIELAQ LISALPRLRL IGLMTIGAPD ASEYSFRALV DARNVIEQAV KLEEKLELSM GMSSDYQLAV KMGADYVRVG SAIFGERTYS QQKGQLQNSD E
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTACCGTT GCCGGCGCTC TCAGAGATAT TAGGGCAGGA TTAGGACACG CAAAGCTCAT TGCTGTCTCA AAGACAAAGC CAGTAGAGGC AATTCTCGAG GCGTATGCGG CCGGCCAGCG TCTGTTTGGA GAGAACTATG TCCAGGAACT AGTGAAAAAG GCTGAGGCCG TGCAGGGCGT GGCAAGCGAT ATTGAGTGGC ACTTTATTGG CCACCTACAA ACTAATAAAG CACGAGACGT CGCCTTCATT CCAAATTGTG TTGTCCAGAC AGTGGATTCT GATAGGCTTG CCAGGCGATT GAGCGAATTG CGACCAGATG AGCTGGGTCC GTTACGGGTG ATGATACAGA TTAACATTAG CGGTGAGCTC ACAAAAAGCG GGTGCACCGT CGAAGATGCA ATTGAATTGG CTCAGTTGAT TAGTGCCCTT CCTCGACTTA GACTTATTGG ACTCATGACC ATAGGAGCCC CAGATGCCTC GGAATACTCC TTCAGAGCAC TTGTGGACGC CCGCAATGTC ATCGAGCAAG CCGTCAAGCT TGAGGAGAAG CTTGAGCTCT CCATGGGGAT GAGTTCCGAC TATCAATTGG CGGTGAAGAT GGGCGCTGAC TACGTCCGAG TTGGATCTGC CATCTTTGGC GAGCGCACTT ATTCACAACA GAAGGGTCAA CTTCAAAATT CGGATGAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA