SSGCID: Target Page

GilaA.01480.a

Kinase, AGC AKT

CENTER ID:	GilaA.01480.a
ORGANISM:	Giardia lamblia ATCC 50803
ASSOCIATED DISEASE:	Giardiasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.01480.a.B2.GE37531	kinase domain		207	586
GilaA.01480.a.B3.GE37532	kinase core		207	490

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
GilaA.01480.a.B2.PW37443	kinase domain		207	586

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	GiardiaDB:GL50803_11364
RefSeq:	XP_001707242.1
UniProt:	E2RTY0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDTDVRIKY AVTKTSSRGK KQPRSLVLTS KSVQNVMGNS IQWERPVQEI YGIHRSTKEP KAFVISFLQT YRFDVESADQ VSSIVSAFQR LNLGEVFVDD KIGAYGLQTD KGKGYKKGHV NVSSDEDGDI GATGAVSQAA DAPASSGQKD RPRTTILEDD LDENIEYVED TRVLAHQIET LPETEVLETF CNPVAAYKFV RPPIFPQVPP SHAKVMRPQN FELVSVVGKG SFGKVFLVRR VEDNRFFAMK VLKKRNVLDR RQLEHLKAEH NILQSFNHPN MIKLYHSFQT RTRLFLVTTF ATGGELFYHL KKIGRFPEPL AVFYLAQIVL VIEYLHKHDI VFRDMKPENL LLDNHGNILL TDFGLSKTGI SADNSGGAGV CSGSFCGTPD YLSGEIISGV PHGKMVDIWA LGALLFEMLV GSAPFSYASS TSTHGGNARS ELYRRILKNI IVFPVFISAE CKSFIAKCLS RRPKDRPNFD EIKKHEVFRK NKIDFDKLYR KEIQPPFKPK LEQSCILAVD GKLLKNPLDN IYRYIAPEAL VVNFDAKFTS ESPILPPSTQ TDSVTMQEDK NFKDFAWTAQ DAFDKL

NT Sequence

atgagcgaca cagacgtgcg tatcaaatat gctgtcacca aaacatctag ccgcgggaaa aagcaacctc gctcactggt gttgacatcg aagtccgtgc agaatgtgat gggtaactcc attcagtggg aaagacccgt tcaggagata tatggaatcc accgctcaac aaaggagccg aaggcctttg tgatctcctt tttgcagacc taccgcttcg atgtcgagtc tgccgaccag gtctcctcga tagtctctgc atttcaaaga ctaaatcttg gcgaggtctt tgttgatgac aagatcggtg cttatggcct ccaaacagac aaaggtaagg gctacaagaa gggtcatgtt aatgtctcat cggatgaaga cggagacatt ggggctactg gagctgtttc tcaggcggca gatgcacctg cttcttcagg tcagaaggat agacctcgca ctacgatact ggaggatgac ctggacgaga atatagagta cgttgaggac acacgtgttc tggcgcacca gatagaaacc cttccagaga cagaagtcct tgaaactttc tgcaaccctg ttgccgccta taaattcgtg cggcccccca tctttccgca ggttcctccc tcgcatgcca aggtgatgag gcctcagaac tttgagcttg tctctgtcgt aggcaagggt tcgttcggta aggtctttct ggttcgtcgc gttgaggaca accgcttctt cgccatgaag gtgcttaaga agcgcaatgt tctggatcgc cgacagctcg agcacctcaa ggccgagcac aacattctgc agagcttcaa ccacccgaac atgatcaagc tctatcactc cttccaaacg cgaacgcgcc tgtttctcgt gacaacgttt gcaactggtg gagagctctt ctatcacctg aagaaaatag ggagatttcc agaacccctt gctgtcttct acctggctca gatagttctc gtgatcgagt acttgcacaa gcacgatatc gtcttcaggg acatgaagcc ggagaacctc cttttagata accatgggaa catcttgctc actgacttcg gtctttccaa gactggcatt tcagctgaca attctggggg cgcaggggtc tgctcgggaa gcttctgcgg cacccctgac tacctctctg gggagattat ctctggcgta ccgcatggca aaatggtaga tatctgggcc ttgggcgccc tgctctttga gatgcttgtt gggtctgcac cgttctccta cgccagttcg acgagtaccc acggaggcaa cgcccgctct gaactatata gacgcatcct taagaatatc atcgtgttcc cggtcttcat cagtgcagag tgtaagagct ttatagctaa atgcttaagc aggcgtccca aggataggcc aaacttcgat gaaatcaaga agcacgaggt cttccgaaag aataagattg atttcgataa gctatacagg aaagagatcc aacctccgtt taagcccaaa cttgagcagt cgtgtatcct tgcggtggac gggaagctac tgaagaatcc tctagacaat atatatcgct acatagcccc ggaagcgctt gtcgtcaact ttgatgctaa gttcacctct gagtctccta ttctgcctcc ttccacacag accgatagcg tcacgatgca agaggataag aatttcaagg actttgcgtg gactgcacag gacgcatttg ataaactata g

Details for GilaA.01480.a.B2.GE37531

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MRPQNFELVS VVGKGSFGKV FLVRRVEDNR FFAMKVLKKR NVLDRRQLEH LKAEHNILQS FNHPNMIKLY HSFQTRTRLF LVTTFATGGE LFYHLKKIGR FPEPLAVFYL AQIVLVIEYL HKHDIVFRDM KPENLLLDNH GNILLTDFGL SKTGISADNS GGAGVCSGSF CGTPDYLSGE IISGVPHGKM VDIWALGALL FEMLVGSAPF SYASSTSTHG GNARSELYRR ILKNIIVFPV FISAECKSFI AKCLSRRPKD RPNFDEIKKH EVFRKNKIDF DKLYRKEIQP PFKPKLEQSC ILAVDGKLLK NPLDNIYRYI APEALVVNFD AKFTSESPIL PPSTQTDSVT MQEDKNFKDF A

Validated NT Sequence

acgcaaagtc cttgaaattc ttatcctctt gcatcgtgac gctatcggtc tgtgtggaag gaggcagaat aggagactca gaggtgaact tagcatcaaa gttgacgaca agcgcttccg gggctatgta gcgatatata ttgtctagag gattcttcag tagcttcccg tccaccgcaa ggatacacga ctgctcaagt ttgggcttaa acggaggttg gatctctttc ctgtatagct tatcgaaatc aatcttattc tttcggaaga cctcgtgctt cttgatttca tcgaagtttg gcctatcctt gggacgcctg cttaagcatt tagctataaa gctcttacac tctgcactga tgaagaccgg gaacacgatg atattcttaa ggatgcgtct atatagttca gagcgggcgt tgcctccgtg ggtactcgtc gaactggcgt aggagaacgg tgcagaccca acaagcatct caaagagcag ggcgcccaag gcccagatat ctaccatttt gccatgcggt acgccagaga taatctcccc agagaggtag tcaggggtgc cgcagaagct tcccgagcag acccctgcgc ccccagaatt gtcagctgaa atgccagtct tggaaagacc gaagtcagtg agcaagatgt tcccatggtt atctaaaagg aggttctccg gcttcatgtc cctgaagacg atatcgtgct tgtgcaagta ctcgatcacg agaactatct gagccaggta gaagacagca aggggttctg gaaatctccc tattttcttc aggtgataga agagctctcc accagttgca aacgttgtca cgagaaacag gcgcgttcgc gtttggaagg agtgatagag cttgatcatg ttcgggtggt tgaagctctg cagaatgttg tgctcggcct tgaggtgctc gagctgtcgg cgatccagaa cattgcgctt cttaagcacc ttcatggcga agaagcggtt gtcctcaacg cgacgaacca gaaagacctt accgaacgaa cccttgccta cgacagagac aagctcaaag ttctgaggcc tcatcacctt ggcatgcgag gga

Expected Protein Sequence

MAHHHHHHQV PPSHAKVMRP QNFELVSVVG KGSFGKVFLV RRVEDNRFFA MKVLKKRNVL DRRQLEHLKA EHNILQSFNH PNMIKLYHSF QTRTRLFLVT TFATGGELFY HLKKIGRFPE PLAVFYLAQI VLVIEYLHKH DIVFRDMKPE NLLLDNHGNI LLTDFGLSKT GISADNSGGA GVCSGSFCGT PDYLSGEIIS GVPHGKMVDI WALGALLFEM LVGSAPFSYA SSTSTHGGNA RSELYRRILK NIIVFPVFIS AECKSFIAKC LSRRPKDRPN FDEIKKHEVF RKNKIDFDKL YRKEIQPPFK PKLEQSCILA VDGKLLKNPL DNIYRYIAPE ALVVNFDAKF TSESPILPPS TQTDSVTMQE DKNFKDFAWT AQDAFDKL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaggttc ctccctcgca tgccaaggtg atgaggcctc agaactttga gcttgtctct gtcgtaggca agggttcgtt cggtaaggtc tttctggttc gtcgcgttga ggacaaccgc ttcttcgcca tgaaggtgct taagaagcgc aatgttctgg atcgccgaca gctcgagcac ctcaaggccg agcacaacat tctgcagagc ttcaaccacc cgaacatgat caagctctat cactccttcc aaacgcgaac gcgcctgttt ctcgtgacaa cgtttgcaac tggtggagag ctcttctatc acctgaagaa aatagggaga tttccagaac cccttgctgt cttctacctg gctcagatag ttctcgtgat cgagtacttg cacaagcacg atatcgtctt cagggacatg aagccggaga acctcctttt agataaccat gggaacatct tgctcactga cttcggtctt tccaagactg gcatttcagc tgacaattct gggggcgcag gggtctgctc gggaagcttc tgcggcaccc ctgactacct ctctggggag attatctctg gcgtaccgca tggcaaaatg gtagatatct gggccttggg cgccctgctc tttgagatgc ttgttgggtc tgcaccgttc tcctacgcca gttcgacgag tacccacgga ggcaacgccc gctctgaact atatagacgc atccttaaga atatcatcgt gttcccggtc ttcatcagtg cagagtgtaa gagctttata gctaaatgct taagcaggcg tcccaaggat aggccaaact tcgatgaaat caagaagcac gaggtcttcc gaaagaataa gattgatttc gataagctat acaggaaaga gatccaacct ccgtttaagc ccaaacttga gcagtcgtgt atccttgcgg tggacgggaa gctactgaag aatcctctag acaatatata tcgctacata gccccggaag cgcttgtcgt caactttgat gctaagttca cctctgagtc tcctattctg cctccttcca cacagaccga tagcgtcacg atgcaagagg ataagaattt caaggacttt gcgtggactg cacaggacgc atttgataaa ctatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for GilaA.01480.a.B3.GE37532

HARVESTED ON:	11/25/2013
SEQUENCED ON:	12/4/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMQ VPPSHAKVMR PQNFELVSVV GKGSFGKVFL VRRVEDNRFF AMKVLKKRNV LDRRQLEHLK AEHNILQSFN HPNMIKLYHS FQTRTRLFLV TTFATGGELF YHLKKIGRFP EPLAVFYLAQ IVLVIEYLHK HDIVFRDMKP ENLLLDNHGN ILLTDFGLSK TGISADNSGG AGVCSGSFCG TPDYLSGEII SGVPHGKMVD IWALGALLFE MLVGSAPFSY ASSTSTHGGN ARSELYRRIL KNIIVFPVFI SAECKSFIAK CLSRRPKDRP NFDEIKKHEV FRK

Validated NT Sequence

tntnnntntt taactttaag aaggagatat accatggctc accaccacca ccaccatatg caggttcctc cctcgcatgc caaggtgatg aggcctcaga actttgagct tgtctctgtc gtaggcaagg gttcgttcgg taaggtcttt ctggttcgtc gcgttgagga caaccgcttc ttcgccatga aggtgcttaa gaagcgcaat gttctggatc gccgacagct cgagcacctc aaggccgagc acaacattct gcagagcttc aaccacccga acatgatcaa gctctatcac tccttccaaa cgcgaacgcg cctgtttctc gtgacaacgt ttgcaactgg tggagagctc ttctatcacc tgaagaaaat agggagattt ccagaacccc ttgctgtctt ctacctggct cagatagttc tcgtgatcga gtacttgcac aagcacgata tcgtcttcag ggacatgaag ccggagaacc tccttttaga taaccatggg aacatcttgc tcactgactt cggtctttcc aagactggca tttcagctga caattctggg ggcgcagggg tctgctcggg aagcttctgc ggcacccctg actacctctc tggggagatt atctctggcg taccgcatgg caaaatggta gatatctggg ccttgggcgc cctgctcttt gagatgcttg ttgggtctgc accgttctcc tacgccagtt cgacgagtac ccacggaggc aacgcccgct ctgaactata tagacgcatc cttaagaata tcatcgtgtt cccggtcttc atcagtgcag agtgtaagag ctttatagct aaatgcttaa gcaggcgtcc caaggatagg ccaaacttcg atgaaatcaa gaagcacgag gtcttccgaa agtaagtgag taagatagga tccnngnnnn cccccccccn nnncgnnggn nntnannnna aaaaaaaang tcgncgtgnn taacggnccc tcctgaanaa ccctctggca aaaattngcg cnnctaccgc gcaactcccn tgccncaacg tnatgacncg gtctctctna ctntggcnat cctnncncnc taccnactan tcgattnggg nncnnacgaa atagganann tacnggncnc agccnnnncc gnnnnnnacn ntntgannac nntanngagt anaatgtcnn nnnnnngngt cccnacnnnn nctnnnctcc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaggttc ctccctcgca tgccaaggtg atgaggcctc agaactttga gcttgtctct gtcgtaggca agggttcgtt cggtaaggtc tttctggttc gtcgcgttga ggacaaccgc ttcttcgcca tgaaggtgct taagaagcgc aatgttctgg atcgccgaca gctcgagcac ctcaaggccg agcacaacat tctgcagagc ttcaaccacc cgaacatgat caagctctat cactccttcc aaacgcgaac gcgcctgttt ctcgtgacaa cgtttgcaac tggtggagag ctcttctatc acctgaagaa aatagggaga tttccagaac cccttgctgt cttctacctg gctcagatag ttctcgtgat cgagtacttg cacaagcacg atatcgtctt cagggacatg aagccggaga acctcctttt agataaccat gggaacatct tgctcactga cttcggtctt tccaagactg gcatttcagc tgacaattct gggggcgcag gggtctgctc gggaagcttc tgcggcaccc ctgactacct ctctggggag attatctctg gcgtaccgca tggcaaaatg gtagatatct gggccttggg cgccctgctc tttgagatgc ttgttgggtc tgcaccgttc tcctacgcca gttcgacgag tacccacgga ggcaacgccc gctctgaact atatagacgc atccttaaga atatcatcgt gttcccggtc ttcatcagtg cagagtgtaa gagctttata gctaaatgct taagcaggcg tcccaaggat aggccaaact tcgatgaaat caagaagcac gaggtcttcc gaaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for GilaA.01480.a.B2.PW37443

PURIFICATION DATe:	2/10/2014
CONCENTRATION:	16.2mg/ml
OBSERVED MW:	42kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAGGTTC CTCCCTCGCA TGCCAAGGTG ATGAGGCCTC AGAACTTTGA GCTTGTCTCT GTCGTAGGCA AGGGTTCGTT CGGTAAGGTC TTTCTGGTTC GTCGCGTTGA GGACAACCGC TTCTTCGCCA TGAAGGTGCT TAAGAAGCGC AATGTTCTGG ATCGCCGACA GCTCGAGCAC CTCAAGGCCG AGCACAACAT TCTGCAGAGC TTCAACCACC CGAACATGAT CAAGCTCTAT CACTCCTTCC AAACGCGAAC GCGCCTGTTT CTCGTGACAA CGTTTGCAAC TGGTGGAGAG CTCTTCTATC ACCTGAAGAA AATAGGGAGA TTTCCAGAAC CCCTTGCTGT CTTCTACCTG GCTCAGATAG TTCTCGTGAT CGAGTACTTG CACAAGCACG ATATCGTCTT CAGGGACATG AAGCCGGAGA ACCTCCTTTT AGATAACCAT GGGAACATCT TGCTCACTGA CTTCGGTCTT TCCAAGACTG GCATTTCAGC TGACAATTCT GGGGGCGCAG GGGTCTGCTC GGGAAGCTTC TGCGGCACCC CTGACTACCT CTCTGGGGAG ATTATCTCTG GCGTACCGCA TGGCAAAATG GTAGATATCT GGGCCTTGGG CGCCCTGCTC TTTGAGATGC TTGTTGGGTC TGCACCGTTC TCCTACGCCA GTTCGACGAG TACCCACGGA GGCAACGCCC GCTCTGAACT ATATAGACGC ATCCTTAAGA ATATCATCGT GTTCCCGGTC TTCATCAGTG CAGAGTGTAA GAGCTTTATA GCTAAATGCT TAAGCAGGCG TCCCAAGGAT AGGCCAAACT TCGATGAAAT CAAGAAGCAC GAGGTCTTCC GAAAGAATAA GATTGATTTC GATAAGCTAT ACAGGAAAGA GATCCAACCT CCGTTTAAGC CCAAACTTGA GCAGTCGTGT ATCCTTGCGG TGGACGGGAA GCTACTGAAG AATCCTCTAG ACAATATATA TCGCTACATA GCCCCGGAAG CGCTTGTCGT CAACTTTGAT GCTAAGTTCA CCTCTGAGTC TCCTATTCTG CCTCCTTCCA CACAGACCGA TAGCGTCACG ATGCAAGAGG ATAAGAATTT CAAGGACTTT GCGTGGACTG CACAGGACGC ATTTGATAAA CTATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT