GilaA.01471.a
Aspartate aminotransferase, cytoplasmic
| CENTER ID: | GilaA.01471.a |
| ORGANISM: | Giardia lamblia ATCC 50803 |
| ASSOCIATED DISEASE: | Giardiasis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.01471.a.A1.GE25748 | Full length( GilaA.01471.a ) | 1 | 427 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| GilaA.01471.a.A1.PW25810 | Full length( GilaA.01471.a ) | 1 | 427 |
Structures
3MEB
DEPOSITED: 3/31/2010
DETERMINATION: XRay
CLONE: GilaA.01471.a.A1.GE25748
PROTEIN: GilaA.01471.a.A1.PW25810
Publications by SSGCID
Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Abendroth J, Choi R, Clifton MC, Edwards TE, Lorimer DD, Lukacs CM, Myler P, Staker BL, van Voorhis W, Wall A
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 566-71
PMID: 25945710; PMCID: PMC4427166
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | GiardiaDB:GL50803_91056 |
| OrthoMCL: | OG5_126737 |
| RefSeq: | XP_001709849.1 |
| UniProt: | A8B1V5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVFSGFPAS PPDAILNLTV LYNADTNPKK VNLGVGAYRD ESGKPWILPA VKEAEAIISS DLSKYNKEYP PVAGFPLFLE AAQFLMFGKD SKAAQEGRIA SCQSLSGTGS LHIGFEFLHL WMPKAEFYMP STTWPNHYGI YDKVFNKLKV PYKEYTYLRK DGELEIDFSN TKKDIQSAPE KSIFLFHACA HNPSGIDFTE AQWKELLPIM KEKKHIAFFD SAYQGFATGS FEADAFAVRM FVDAGVEVLV AQSFSKNFGL YGERIGCLHV VHAGVEGSVE KNKALSAAMV SGMTLQIRKT WSMSAIHGAY IVQVIVHDKR LLQMFYDNVK EMSARIHRMR SLLHASLAKR KTPGPGSKGT WDHILTAIGM FTFTGLTPEH VDYLKEKWSI YLVKAGGRMS MCGLTESNCD YVAEAIHDAV TKLPFKK
NT Sequence
ATGTCTGTCT TCTCAGGGTT TCCTGCTTCC CCACCAGATG CTATCCTGAA TCTCACGGTA CTCTACAACG CCGATACCAA CCCGAAAAAG GTGAACCTGG GGGTTGGCGC GTATCGTGAC GAGAGCGGTA AACCATGGAT TCTGCCGGCC GTGAAGGAGG CAGAGGCCAT TATATCTTCC GATCTTTCGA AGTATAATAA GGAGTATCCT CCAGTCGCCG GATTTCCTCT GTTTCTGGAG GCCGCCCAGT TCCTGATGTT CGGCAAGGAC AGCAAGGCAG CCCAGGAGGG TCGCATTGCT AGCTGCCAGT CTCTCTCTGG CACCGGCTCT CTCCACATCG GGTTTGAGTT CCTGCACCTC TGGATGCCAA AGGCAGAGTT TTACATGCCG AGCACAACGT GGCCCAACCA CTACGGAATA TACGATAAGG TGTTCAACAA ATTGAAGGTG CCATACAAGG AGTATACGTA TCTTCGTAAA GACGGAGAGC TTGAGATAGA CTTTTCCAAC ACGAAGAAGG ACATCCAGTC GGCTCCAGAG AAAAGCATAT TCCTGTTCCA CGCCTGTGCC CACAACCCGT CTGGGATCGA CTTCACTGAG GCTCAGTGGA AGGAGCTCCT TCCGATCATG AAGGAGAAGA AGCACATTGC CTTTTTCGAT TCAGCATACC AGGGCTTTGC TACGGGCTCC TTTGAGGCCG ACGCCTTCGC TGTGCGCATG TTTGTCGATG CGGGGGTAGA GGTCCTTGTC GCCCAGAGCT TCTCCAAGAA CTTCGGGCTC TATGGAGAGC GTATCGGCTG CCTACATGTT GTCCATGCAG GAGTGGAGGG GTCCGTCGAG AAGAACAAGG CGCTTTCTGC TGCTATGGTG AGTGGAATGA CTCTCCAGAT CCGTAAGACG TGGTCCATGT CTGCGATCCA CGGGGCCTAT ATCGTCCAAG TCATCGTTCA CGACAAGCGC CTTCTGCAGA TGTTTTATGA CAATGTCAAG GAGATGAGTG CTCGCATTCA CCGAATGCGT AGTCTCCTGC ATGCCTCCCT TGCGAAGCGC AAGACTCCAG GCCCCGGTTC CAAAGGGACC TGGGACCATA TACTGACAGC CATCGGCATG TTCACTTTCA CCGGGCTCAC CCCAGAGCAC GTTGATTATC TGAAGGAGAA GTGGAGTATC TATCTTGTGA AGGCAGGGGG ACGCATGAGT ATGTGCGGGC TCACTGAGTC TAATTGTGAC TATGTGGCTG AGGCGATTCA CGACGCCGTC ACGAAGCTCC CGTTCAAGAA A
Details for GilaA.01471.a.A1.GE25748
| HARVESTED ON: | 10/31/2008 |
| SEQUENCED ON: | 7/20/2010 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMSVFSGF PASPPDAILN LTVLYNADTN PKKVNLGVGA YRDESGKPWI LPAVKEAEAI ISSDLSKYNK EYPPVAGFPL FLEAAQFLMF GKDSKAAQEG RIASCQSLSG TGSLHIGFEF LHLWMPKAEF YMPSTTWPNH YGIYDKVFNK LKVPYKEYTY LRKDGELEID FSNTKKDIQS APEKSIFLFH ACAHNPSGID FTEAQWKELL PIMKEKKHIA FFDSAYQGFA TGSFEADAFA VRMFVDAGVE VLVAQSFSKN FGLYGERIGC LHVVHAGVEG SVEKNKALSA AMVSGMTLQI RKTWSMSAIH GAYIVQVIVH DKRLLQMFYD NVKEMSARIH RMRSLLHASL AKRKTPGPGS KGTWDHILTA IGMFTFTGLT PEHVDYLKEK WSIYLVKAGG RMSMCGLTES NCDYVAEAIH DAVTKLPF
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctgt cttctcaggg tttcctgctt ccccaccaga tgctatcctg aatctcacgg tactctacaa cgccgatacc aacccgaaaa aggtgaacct gggggttggc gcgtatcgtg acgagagcgg taaaccatgg attctgccgg ccgtgaagga ggcagaggcc attatatctt ccgatctttc gaagtataat aaggagtatc ctccagtcgc cggatttcct ctgtttctgg aggccgccca gttcctgatg ttcggcaagg acagcaaggc agcccaggag ggtcgcattg ctagctgcca gtctctctct ggcaccggct ctctccacat cgggtttgag ttcctgcacc tctggatgcc aaaggcagag ttttacatgc cgagcacaac gtggcccaac cactacggaa tatacgataa ggtgttcaac aaattgaagg tgccatacaa ggagtatacg tatcttcgta aagacggaga gcttgagata gacttttcca acacgaagaa ggacatccag tcggctccag agaaaagcat attcctgttc cacgcctgtg cccacaaccc gtctgggatc gacttcactg aggctcagtg gaaggagctc cttccgatca tgaaggagaa gaagcacatt gcctttttcg attcagcata ccagggcttt gctacgggct cctttgaggc ngacgccttc gctgtgcgca tgtttgtcga tgcgggggta gaggtccttg tcgcccagag cttctccaag aacttcgggc tctatggaga gcgtatcggc tgcctacatg ttgtccatgc aggagtggag gggtccgtcg agaagaacaa ggcgctttct gctgctatgg tgagtggaat gactctccag atccgtaaga cgtggtccat gtctgcgatc cacggggcct atatcgtcca agtcatcgtt cacgacaagc gccttctgca gatgttttat gacaatgtca aggagatgag tgctcgcatt caccgaatgc gtagtctcct gcatgcctcc cttgcgaagc gcaagactcc aggccccggt tccaaaggga cctgggacca tatactgaca gccatcggca tgttcacttt caccgggctc accccagagc acgttgatta tctgaaggag aagtggagta tctatcttgt gaaggcaggg ggacgcatga gtatgtgcgg gctcactgag tctaattgtg actatgtggc tgaggcgatt cacgacgccg tcacgaagct cccgttca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVFSGFPA SPPDAILNLT VLYNADTNPK KVNLGVGAYR DESGKPWILP AVKEAEAIIS SDLSKYNKEY PPVAGFPLFL EAAQFLMFGK DSKAAQEGRI ASCQSLSGTG SLHIGFEFLH LWMPKAEFYM PSTTWPNHYG IYDKVFNKLK VPYKEYTYLR KDGELEIDFS NTKKDIQSAP EKSIFLFHAC AHNPSGIDFT EAQWKELLPI MKEKKHIAFF DSAYQGFATG SFEADAFAVR MFVDAGVEVL VAQSFSKNFG LYGERIGCLH VVHAGVEGSV EKNKALSAAM VSGMTLQIRK TWSMSAIHGA YIVQVIVHDK RLLQMFYDNV KEMSARIHRM RSLLHASLAK RKTPGPGSKG TWDHILTAIG MFTFTGLTPE HVDYLKEKWS IYLVKAGGRM SMCGLTESNC DYVAEAIHDA VTKLPFKK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgtcttc tcagggtttc ctgcttcccc accagatgct atcctgaatc tcacggtact ctacaacgcc gataccaacc cgaaaaaggt gaacctgggg gttggcgcgt atcgtgacga gagcggtaaa ccatggattc tgccggccgt gaaggaggca gaggccatta tatcttccga tctttcgaag tataataagg agtatcctcc agtcgccgga tttcctctgt ttctggaggc cgcccagttc ctgatgttcg gcaaggacag caaggcagcc caggagggtc gcattgctag ctgccagtct ctctctggca ccggctctct ccacatcggg tttgagttcc tgcacctctg gatgccaaag gcagagtttt acatgccgag cacaacgtgg cccaaccact acggaatata cgataaggtg ttcaacaaat tgaaggtgcc atacaaggag tatacgtatc ttcgtaaaga cggagagctt gagatagact tttccaacac gaagaaggac atccagtcgg ctccagagaa aagcatattc ctgttccacg cctgtgccca caacccgtct gggatcgact tcactgaggc tcagtggaag gagctccttc cgatcatgaa ggagaagaag cacattgcct ttttcgattc agcataccag ggctttgcta cgggctcctt tgaggccgac gccttcgctg tgcgcatgtt tgtcgatgcg ggggtagagg tccttgtcgc ccagagcttc tccaagaact tcgggctcta tggagagcgt atcggctgcc tacatgttgt ccatgcagga gtggaggggt ccgtcgagaa gaacaaggcg ctttctgctg ctatggtgag tggaatgact ctccagatcc gtaagacgtg gtccatgtct gcgatccacg gggcctatat cgtccaagtc atcgttcacg acaagcgcct tctgcagatg ttttatgaca atgtcaagga gatgagtgct cgcattcacc gaatgcgtag tctcctgcat gcctcccttg cgaagcgcaa gactccaggc cccggttcca aagggacctg ggaccatata ctgacagcca tcggcatgtt cactttcacc gggctcaccc cagagcacgt tgattatctg aaggagaagt ggagtatcta tcttgtgaag gcagggggac gcatgagtat gtgcgggctc actgagtcta attgtgacta tgtggctgag gcgattcacg acgccgtcac gaagctcccg ttcaagaaaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for GilaA.01471.a.A1.PW25810
| PURIFICATION DATe: | 2/10/2009 |
| CONCENTRATION: | 34.78mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 55 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSVFSGF PASPPDAILN LTVLYNADTN PKKVNLGVGA YRDESGKPWI LPAVKEAEAI ISSDLSKYNK EYPPVAGFPL FLEAAQFLMF GKDSKAAQEG RIASCQSLSG TGSLHIGFEF LHLWMPKAEF YMPSTTWPNH YGIYDKVFNK LKVPYKEYTY LRKDGELEID FSNTKKDIQS APEKSIFLFH ACAHNPSGID FTEAQWKELL PIMKEKKHIA FFDSAYQGFA TGSFEADAFA VRMFVDAGVE VLVAQSFSKN FGLYGERIGC LHVVHAGVEG SVEKNKALSA AMVSGMTLQI RKTWSMSAIH GAYIVQVIVH DKRLLQMFYD NVKEMSARIH RMRSLLHASL AKRKTPGPGS KGTWDHILTA IGMFTFTGLT PEHVDYLKEK WSIYLVKAGG RMSMCGLTES NCDYVAEAIH DAVTKLPF
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctgt cttctcaggg tttcctgctt ccccaccaga tgctatcctg aatctcacgg tactctacaa cgccgatacc aacccgaaaa aggtgaacct gggggttggc gcgtatcgtg acgagagcgg taaaccatgg attctgccgg ccgtgaagga ggcagaggcc attatatctt ccgatctttc gaagtataat aaggagtatc ctccagtcgc cggatttcct ctgtttctgg aggccgccca gttcctgatg ttcggcaagg acagcaaggc agcccaggag ggtcgcattg ctagctgcca gtctctctct ggcaccggct ctctccacat cgggtttgag ttcctgcacc tctggatgcc aaaggcagag ttttacatgc cgagcacaac gtggcccaac cactacggaa tatacgataa ggtgttcaac aaattgaagg tgccatacaa ggagtatacg tatcttcgta aagacggaga gcttgagata gacttttcca acacgaagaa ggacatccag tcggctccag agaaaagcat attcctgttc cacgcctgtg cccacaaccc gtctgggatc gacttcactg aggctcagtg gaaggagctc cttccgatca tgaaggagaa gaagcacatt gcctttttcg attcagcata ccagggcttt gctacgggct cctttgaggc ngacgccttc gctgtgcgca tgtttgtcga tgcgggggta gaggtccttg tcgcccagag cttctccaag aacttcgggc tctatggaga gcgtatcggc tgcctacatg ttgtccatgc aggagtggag gggtccgtcg agaagaacaa ggcgctttct gctgctatgg tgagtggaat gactctccag atccgtaaga cgtggtccat gtctgcgatc cacggggcct atatcgtcca agtcatcgtt cacgacaagc gccttctgca gatgttttat gacaatgtca aggagatgag tgctcgcatt caccgaatgc gtagtctcct gcatgcctcc cttgcgaagc gcaagactcc aggccccggt tccaaaggga cctgggacca tatactgaca gccatcggca tgttcacttt caccgggctc accccagagc acgttgatta tctgaaggag aagtggagta tctatcttgt gaaggcaggg ggacgcatga gtatgtgcgg gctcactgag tctaattgtg actatgtggc tgaggcgatt cacgacgccg tcacgaagct cccgttca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVFSGFPA SPPDAILNLT VLYNADTNPK KVNLGVGAYR DESGKPWILP AVKEAEAIIS SDLSKYNKEY PPVAGFPLFL EAAQFLMFGK DSKAAQEGRI ASCQSLSGTG SLHIGFEFLH LWMPKAEFYM PSTTWPNHYG IYDKVFNKLK VPYKEYTYLR KDGELEIDFS NTKKDIQSAP EKSIFLFHAC AHNPSGIDFT EAQWKELLPI MKEKKHIAFF DSAYQGFATG SFEADAFAVR MFVDAGVEVL VAQSFSKNFG LYGERIGCLH VVHAGVEGSV EKNKALSAAM VSGMTLQIRK TWSMSAIHGA YIVQVIVHDK RLLQMFYDNV KEMSARIHRM RSLLHASLAK RKTPGPGSKG TWDHILTAIG MFTFTGLTPE HVDYLKEKWS IYLVKAGGRM SMCGLTESNC DYVAEAIHDA VTKLPFKK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTGTCTTC TCAGGGTTTC CTGCTTCCCC ACCAGATGCT ATCCTGAATC TCACGGTACT CTACAACGCC GATACCAACC CGAAAAAGGT GAACCTGGGG GTTGGCGCGT ATCGTGACGA GAGCGGTAAA CCATGGATTC TGCCGGCCGT GAAGGAGGCA GAGGCCATTA TATCTTCCGA TCTTTCGAAG TATAATAAGG AGTATCCTCC AGTCGCCGGA TTTCCTCTGT TTCTGGAGGC CGCCCAGTTC CTGATGTTCG GCAAGGACAG CAAGGCAGCC CAGGAGGGTC GCATTGCTAG CTGCCAGTCT CTCTCTGGCA CCGGCTCTCT CCACATCGGG TTTGAGTTCC TGCACCTCTG GATGCCAAAG GCAGAGTTTT ACATGCCGAG CACAACGTGG CCCAACCACT ACGGAATATA CGATAAGGTG TTCAACAAAT TGAAGGTGCC ATACAAGGAG TATACGTATC TTCGTAAAGA CGGAGAGCTT GAGATAGACT TTTCCAACAC GAAGAAGGAC ATCCAGTCGG CTCCAGAGAA AAGCATATTC CTGTTCCACG CCTGTGCCCA CAACCCGTCT GGGATCGACT TCACTGAGGC TCAGTGGAAG GAGCTCCTTC CGATCATGAA GGAGAAGAAG CACATTGCCT TTTTCGATTC AGCATACCAG GGCTTTGCTA CGGGCTCCTT TGAGGCCGAC GCCTTCGCTG TGCGCATGTT TGTCGATGCG GGGGTAGAGG TCCTTGTCGC CCAGAGCTTC TCCAAGAACT TCGGGCTCTA TGGAGAGCGT ATCGGCTGCC TACATGTTGT CCATGCAGGA GTGGAGGGGT CCGTCGAGAA GAACAAGGCG CTTTCTGCTG CTATGGTGAG TGGAATGACT CTCCAGATCC GTAAGACGTG GTCCATGTCT GCGATCCACG GGGCCTATAT CGTCCAAGTC ATCGTTCACG ACAAGCGCCT TCTGCAGATG TTTTATGACA ATGTCAAGGA GATGAGTGCT CGCATTCACC GAATGCGTAG TCTCCTGCAT GCCTCCCTTG CGAAGCGCAA GACTCCAGGC CCCGGTTCCA AAGGGACCTG GGACCATATA CTGACAGCCA TCGGCATGTT CACTTTCACC GGGCTCACCC CAGAGCACGT TGATTATCTG AAGGAGAAGT GGAGTATCTA TCTTGTGAAG GCAGGGGGAC GCATGAGTAT GTGCGGGCTC ACTGAGTCTA ATTGTGACTA TGTGGCTGAG GCGATTCACG ACGCCGTCAC GAAGCTCCCG TTCAAGAAAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A